scholarly journals Antibacterial, antifungal and cytotoxic activities exhibited by endophytic fungi from the Brazilian marine red alga Bostrychia tenella (Ceramiales)

2015 ◽  
Vol 25 (6) ◽  
pp. 641-650 ◽  
Author(s):  
Rafael de Felício ◽  
Gabriel B. Pavão ◽  
Ana Lígia L. de Oliveira ◽  
Cíntia Erbert ◽  
Raphael Conti ◽  
...  
Keyword(s):  
Endophytic Fungi ◽  
Red Alga ◽  
Cytotoxic Activities

Cytotoxic Activities of Endophytic Fungi Isolated from the Endangered, Chinese Endemic Species Dysosma pleiantha

2009 ◽  
Vol 64 (7-8) ◽  
pp. 518-520 ◽  
Author(s):  
Yin Lu ◽  
Shaoyuan Chen ◽  
Ben Wang
Keyword(s):  
Medicinal Plant ◽  
Cytotoxic Activity ◽  
Endophytic Fungi ◽  
Endemic Species ◽  
Brine Shrimp ◽  
Cytotoxic Activities ◽  
Antitumour Agents ◽  
Endemic Medicinal Plant ◽  
Trichoderma Citrinoviride ◽  
Promising Source

Eleven strains of endophytic fungi which habitat in an endangered, Chinese endemic medicinal plant, Dysosma pleiantha (Hance) Woodson, were isolated and tested for their cytotoxic activity using the brine shrimp lethality bioassay. Six isolates were found to exhibit some cytotoxic activity. Extracts of F1273, F1276, and F1280, which were identified as Trichoderma citrinoviride, Chaetomium globosum and Ascomycete sp., in particular, showed most potent activity with LC50 values of 4.86, 7.71, and 14.88 μg/ml, respectively. These results indicate that endophytic fungi of Dysosma pleiantha could be a promising source for antitumour agents.

scholarly journals Antimicrobial and Cytotoxic Activities of Endophytic Fungi Isolated from Artemisia annua L

2014 ◽  
Vol 4 (10) ◽  
pp. 47-50 ◽  
Author(s):  
Puji Astuti ◽  
Wahyono Wahyono ◽  
Titik Nuryastuti ◽  
Indah Purwantini ◽  
Purwanto Purwanto
Keyword(s):  
Endophytic Fungi ◽  
Artemisia Annua ◽  
Cytotoxic Activities ◽  
Artemisia Annua L

Bioactive potential of endophytic fungi isolated from Phyllanthus niruri L.

2020 ◽  
Vol 55 (4) ◽  
pp. 311-318
Author(s):  
SU Hannana ◽  
F Afroz ◽  
MN Begum ◽  
S Sharmin ◽  
F Moni ◽  
...  
Keyword(s):  
Endophytic Fungi ◽  
Morphological Identification ◽  
Cytotoxic Activities ◽  
Crystal Formation ◽  
White Crystal ◽  
Phyllanthus Niruri ◽  
Chemical Screening ◽  
Activity Screening ◽  
Bioactive Potential ◽  
Promising Source

Present work describes the isolation and morphological identification of endophytic fungi from Phyllanthus niruri L. including their chemical and bioactivity screening. Two isolated and purified endophytic fungi with the internal strain numbers PNRE and PNLE were taxonomically identified as Colletotrichum sp. and Fusarium sp., respectively. Preliminary chemical screening of the fungal extracts through thin layer chromatography (TLC) revealed the presence of various components. PNLE showed significant antifungal activity against the fungi Aspergillus niger (24 mm) and Aspergillus flavus (20 mm). Extracts of PNRE and PNLE gave significant cytotoxic activity with LC50 values of 1.08 μg mL-1 and 1.05 μg mL-1, respectively in the brine shrimp lethality bioassay. PNRE extract showed mild scavenging of DPPH with IC50 value of 96.06 μg mL-1 during antioxidant activity screening. Crystal formation was observed in the crude PNRE fungal extract which was purified using methanol/dichloromethane (0-20%) to obtain pure white crystal and the structure was elucidated by NMR (1H NMR and 13C NMR) spectroscopic analysis to be confirmed as ergosterol. Since isolated fungi showed promising antifungal and cytotoxic activities, the crude extract of endophytic fungi of P. niruri could be considered as a promising source for isolation of potential bioactive compounds. Bangladesh J. Sci. Ind. Res.55(4), 311-318, 2020

scholarly journals Bioreduction of fluoroacetophenone derivatives by endophytic fungi isolated from the marine red alga Bostrychia radicans

Biocatalysis ◽  
2016 ◽  
Vol 1 (1) ◽  
Author(s):  
Ana Maria Mouad ◽  
Ana Lígia L. de Oliveira ◽  
Hosana Maria Debonsi ◽  
André Luiz Meleiro Porto
Keyword(s):  
Endophytic Fungi ◽  
Marine Fungi ◽  
Red Alga ◽  
Chiral Alcohols ◽  
Reduction Reactions ◽  
Asymmetric Bioreduction

AbstractFour endophytic fungi isolated from the marine red alga Bostrychia radicans identified as Botryosphaeria sp. CBMAI 1197, Eutypella sp. CBMAI 1196, Hidropisphaera sp. CBMAI 1194 and Xylaria sp. CBMAI 1195 catalyzed the asymmetric bioreduction of fluoroacetophenone derivatives 1-3 to the corresponding fluorophenylalcohols 1a-3a. In the reduction reactions of 2,2,2-trifluoro-1-phenylethanone 1, all the marine fungi produced exclusively the (S)-2,2,2-trifluoro- 1-phenylethanol 1a with > 99% ee. The fungus Botryosphaeria sp. CBMAI 1197 exhibited the best enzymatic potential, leading to the highest conversion values (up to > 99%). The biocatalyst Botryosphaeria sp. CBMAI 1197 also presented active enzymes in reactions with the substrates 1-(2-(trifluoromethyl)phenyl) ethanone (2) and 1-(2,4,5-trifluorophenyl)ethanone (3), producing the respective chiral alcohols S-2a and R-3a with > 99% ee. Additionally, the fungus Hidropisphaera sp. CBMAI 1194 yielded 100% of conversion of the ketone 3 to the corresponding S-alcohol 3a, with 53% ee.

scholarly journals ANTIMICROBIAL AND CYTOTOXIC ACTIVITIES OF ALKALOIDS PRODUCED BY ENDOPHYTIC FUNGI

2018 ◽  
pp. 50-59
Author(s):  
Amarbayasgalan M ◽  
Nomin M ◽  
Enkh-Amgalan J
Keyword(s):  
Liver Cancer ◽  
Endophytic Fungi ◽  
Morphological Characteristics ◽  
Cytotoxic Activities ◽  
Rrna Gene ◽  
28S Rrna ◽  
Fungal Strains ◽  
Liver Cancer Cells ◽  
D2 Domain ◽  
Tlc Analysis

In this study, 55 endophytic fungal strains isolated from alkaloid containing plants in Mongolia were screened for their production of alkaloids. TLC analysis of ethanolic crude extracts revealed the presence of alkaloids in 5 endophytic fungal strains. The alkaloid producing strains were identified based on their morphological characteristics and/or the D1/D2 domain sequences of 28S rRNA gene. The results showed that they all belong to the genus of Fusarium. Further, total alkaloid compounds of the 5 fungal strains were isolated and examined for their antimicrobial and cytotoxic activities. As results, alkaloid compounds of SFG-S1 and 16T6-F3 strains were active against yeast and fungi, and alkaloid compounds of SFG-R3, 16Т6-F3, 14R-1 strains exhibited cytotoxic activity against HepG2 liver cancer cells.

scholarly journals Aromatic compounds produced by endophytic fungi isolated from red alga Asparagopsis taxiformis - Falkenbergia stage

2018 ◽  
Vol 33 (3) ◽  
pp. 443-446 ◽  
Author(s):  
Rebeca P. Medina ◽  
Angela R. Araujo ◽  
Raymond J. Andersen ◽  
Marcos A. Soares ◽  
Fabio de A. Silva ◽  
...  
Keyword(s):  
Endophytic Fungi ◽  
Aromatic Compounds ◽  
Red Alga ◽  
Asparagopsis Taxiformis

Bioactivity Screening of Endophytic Fungus Eutypa linearis isolated from Coleus amboinicus (Lour.)

2021 ◽  
pp. 86-95
Author(s):  
Baiq Maylinda Gemantari ◽  
Fitra Romadhonsyah ◽  
Arief Nurrochmad ◽  
Subagus Wahyuono ◽  
Puji Astuti
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Endophytic Fungi ◽  
Endophytic Fungus ◽  
Fermentation Broth ◽  
Cytotoxic Activities ◽  
Dpph Assay ◽  
Nitrobenzyl Alcohol ◽  
Layer Chromatography ◽  
Coleus Amboinicus

Coleus amboinicus (Lour.) is a medicinal plant containing various bioactive compounds. Endophytes are microorganisms living inside intracellular tissue of plants and known as the source of bioactive compounds. In order to explore the potential of endophyte in producing novel bioactive compounds, this study focused on isolating endophytic fungi from the leaves of C.amboinicus, characterisation and screening their metabolite bioactivity during submerged culture fermentation. Isolation of endophytic fungi from the leaves segment of C.amboinicus was conducted on PDA media and fungus identification was carried out by analyzing its morphology and molecular examination. The production of metabolites was examined using thin layer chromatography and gas chromatography. Identification of the endophytic fungus showed 98.84% similarity with Eutypa linearis. This species was fermented submergedly in PDB medium for 14 days under dark and exposed to light and the fermentation broth was extracted using ethyl acetate. The results showed that exposure to the light did not significantly influence metabolite production. The ethyl acetate extract exhibited antioxidant and cytotoxic activities. Antioxidant activity of this extract as examined by DPPH assay showed IC50 of 105.31 ± 2.11 µg/mL. Cytotoxic activity against Hela cell line was known to be the best among other cell lines (IC50 301.53 ± 11.34 µg/mL) although it was found to be non selective (SI<1). The extract contained five major compounds namely Benzenemethanol, 4-nitro-(CAS) p-Nitrobenzyl alcohol; 2-Pentadecanone (CAS) Pentadecan-2-one; (1R*,6S*,10R*)-5,5-Dimethyl-11,12-dioxatricyclo[8.2.1.0(1,6)] tridecan-10-ol; 9,12-Octadecadienoic acid (Z,Z)-, methyl ester (CAS) Methyl linoleate; and 3-Furanacetic acid, 4-hexyl-2,5-dihydro-2,5-dioxo- (CAS) 2-carboxymethyl-3-N-hexyl-maleic anhydride.

scholarly journals Antioxidant and Cytotoxicity Activities of the Fermentation Extract of the Endophytic Fungi from the Marine Biota of Colt Coral

ALCHEMY ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 63-72
Author(s):  
Dede Sukandar ◽  
Nina Artanti ◽  
Ika Restu Purwanti ◽  
Tarso Rudiana ◽  
Fitriyanti Fitriyanti
Keyword(s):  
Endophytic Fungi ◽  
Hydroxyl Group ◽  
Radical Scavenger ◽  
Cytotoxic Activities ◽  
Hydroxyl Groups ◽  
Marine Biota ◽  
Cytotoxic Assay ◽  
Fermentation Time ◽  
Antioxidant Assay ◽  
Dpph Method

Indonesia has the largest and most biodiverse coral reef in the world. Colt coral has not been studied and explored especially endophytic fungi associated with the coral. Endophytic fungi are highly potential for the production of antioxidant and anticancer compounds. This research aimed to study the antioxidant and cytotoxic activities of fermentation extract from endophytic fungi from colt coral. Filtrate and mycelium extracts were obtained from static and shake fermentations of isolate SKF 15. Antioxidant and cytotoxic assays were conducted by free radical scavenger 1,1-diphenyl-2-picrylhydrazyl (DPPH) and AlamarBlue methods, respectively. The result showed that FD extract provided the highest antioxidant activity with inhibition of 49.36% at 200 ppm of DPPH. Variation of fermentation time (3-21 days) demonstrated the highest activity with inhibition of 66.97% for antioxidant assay (7 days) and 81.13% for cytotoxic assay (3 days). FTIR analysis presented the existence of hydroxyl groups O-H (3452.58 cm-1), C=C groups (1668.43 cm-1); C-O hydroxyl group (1230.58 cm -1), and C-H sp3 groups (2941.44 cm-1). Based on LC-MS analysis, FD extract has a mass of m/z 305.63, [M+H]+, predicted as dihydroquercetin (C15H24O7). Keywords: Antioxidant assay, cytotoxic assay, endophytic fungi, colt coral, DPPH method, AlamarBlue method

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