Bioactive potential of endophytic fungi isolated from Phyllanthus niruri L.

2020 ◽  
Vol 55 (4) ◽  
pp. 311-318
Author(s):  
SU Hannana ◽  
F Afroz ◽  
MN Begum ◽  
S Sharmin ◽  
F Moni ◽  
...  
Keyword(s):  
Endophytic Fungi ◽  
Morphological Identification ◽  
Cytotoxic Activities ◽  
Crystal Formation ◽  
White Crystal ◽  
Phyllanthus Niruri ◽  
Chemical Screening ◽  
Activity Screening ◽  
Bioactive Potential ◽  
Promising Source

Present work describes the isolation and morphological identification of endophytic fungi from Phyllanthus niruri L. including their chemical and bioactivity screening. Two isolated and purified endophytic fungi with the internal strain numbers PNRE and PNLE were taxonomically identified as Colletotrichum sp. and Fusarium sp., respectively. Preliminary chemical screening of the fungal extracts through thin layer chromatography (TLC) revealed the presence of various components. PNLE showed significant antifungal activity against the fungi Aspergillus niger (24 mm) and Aspergillus flavus (20 mm). Extracts of PNRE and PNLE gave significant cytotoxic activity with LC50 values of 1.08 μg mL-1 and 1.05 μg mL-1, respectively in the brine shrimp lethality bioassay. PNRE extract showed mild scavenging of DPPH with IC50 value of 96.06 μg mL-1 during antioxidant activity screening. Crystal formation was observed in the crude PNRE fungal extract which was purified using methanol/dichloromethane (0-20%) to obtain pure white crystal and the structure was elucidated by NMR (1H NMR and 13C NMR) spectroscopic analysis to be confirmed as ergosterol. Since isolated fungi showed promising antifungal and cytotoxic activities, the crude extract of endophytic fungi of P. niruri could be considered as a promising source for isolation of potential bioactive compounds. Bangladesh J. Sci. Ind. Res.55(4), 311-318, 2020

Cytotoxic Activities of Endophytic Fungi Isolated from the Endangered, Chinese Endemic Species Dysosma pleiantha

2009 ◽  
Vol 64 (7-8) ◽  
pp. 518-520 ◽  
Author(s):  
Yin Lu ◽  
Shaoyuan Chen ◽  
Ben Wang
Keyword(s):  
Medicinal Plant ◽  
Cytotoxic Activity ◽  
Endophytic Fungi ◽  
Endemic Species ◽  
Brine Shrimp ◽  
Cytotoxic Activities ◽  
Antitumour Agents ◽  
Endemic Medicinal Plant ◽  
Trichoderma Citrinoviride ◽  
Promising Source

Eleven strains of endophytic fungi which habitat in an endangered, Chinese endemic medicinal plant, Dysosma pleiantha (Hance) Woodson, were isolated and tested for their cytotoxic activity using the brine shrimp lethality bioassay. Six isolates were found to exhibit some cytotoxic activity. Extracts of F1273, F1276, and F1280, which were identified as Trichoderma citrinoviride, Chaetomium globosum and Ascomycete sp., in particular, showed most potent activity with LC50 values of 4.86, 7.71, and 14.88 μg/ml, respectively. These results indicate that endophytic fungi of Dysosma pleiantha could be a promising source for antitumour agents.

scholarly journals Screening Fungal Endophytes Derived from Under-Explored Egyptian Marine Habitats for Antimicrobial and Antioxidant Properties in Factionalised Textiles

2020 ◽  
Vol 8 (10) ◽  
pp. 1617
Author(s):  
Ahmed A. Hamed ◽  
Sylvia Soldatou ◽  
M. Mallique Qader ◽  
Subha Arjunan ◽  
Kevin Jace Miranda ◽  
...  
Keyword(s):  
Endophytic Fungi ◽  
Pathogenic Bacteria ◽  
Antioxidant Activities ◽  
Human Fibroblasts ◽  
Fungal Endophytes ◽  
Antioxidant Properties ◽  
Chemical Screening ◽  
Marine Habitats ◽  
Bacteria And Fungi ◽  
Promising Source

Marine endophytic fungi from under-explored locations are a promising source for the discovery of new bioactivities. Different endophytic fungi were isolated from plants and marine organisms collected from Wadi El-Natrun saline lakes and the Red Sea near Hurghada, Egypt. The isolated strains were grown on three different media, and their ethyl acetate crude extracts were evaluated for their antimicrobial activity against a panel of pathogenic bacteria and fungi as well as their antioxidant properties. Results showed that most of the 32 fungal isolates initially obtained possessed antimicrobial and antioxidant activities. The most potent antimicrobial extracts were applied to three different cellulose containing fabrics to add new multifunctional properties such as ultraviolet protection and antimicrobial functionality. For textile safety, the toxicity profile of the selected fungal extract was evaluated on human fibroblasts. The 21 strains displaying bioactivity were identified on molecular basis and selected for chemical screening and dereplication, which was carried out by analysis of the MS/MS data using the Global Natural Products Social Molecular Networking (GNPS) platform. The obtained molecular network revealed molecular families of compounds commonly produced by fungal strains, and in combination with manual dereplication, further previously reported metabolites were identified as well as potentially new derivatives.

scholarly journals Bioactivity Screening and Identification of Secondary Metabolites from Fungal Endophytes of Carica papaya L. Leaves

2021 ◽  
Vol 20 (4) ◽  
pp. 217
Author(s):  
Israt Farha Lini ◽  
Rabita Zinnurine ◽  
Md. Habibur Rahman ◽  
Mst. Nadira Begum ◽  
Farhana Afroz ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Large Scale ◽  
Carica Papaya ◽  
Fungal Endophytes ◽  
Diffusion Method ◽  
Cytotoxic Activities ◽  
Alternariol Monomethyl Ether ◽  
Chemical Screening

<strong>Objectives:</strong> This work describes the chemical and <em>in-vitro</em> bioactivity studies on endophytic fungi derived from the leaves of <em>Carica papaya</em> L. <strong>Methods:</strong> Column Chromatography was employed for isolation of fungal metabolites. Nuclear Magnetic Resonance (NMR) spectral data was utilized to elucidate the structure of the isolated compounds and physicochemical properties of them were also examined. As a part of<em> in-vitro</em> bioactivity screening disc diffusion method, 1, 1-diphenyl-2- picryl-hydrazyl (DPPH) scavenging assay and brine shrimp lethality bioassay were conducted to evaluate antimicrobial, antioxidant and cytotoxic activities, respectively.<strong> Results:</strong> A total of four fungal isolates were identified as endophytic fungi and purified from the young leaves of <em>Carica papaya</em>. These fungi, encoded as CPLE-1, CPLE-2, CPLE-3 and CPLE-4, were identified up to the genus level on the basis of their macroscopic and microscopic characteristics as <em>Colletotrichum</em> sp., <em>Carvularia</em> sp., and next two as <em>Alternaria</em> sp., respectively. Chemical profiling and preliminary bioactivity screening of the fungal endophytes signified strain CPLE-3 with highest potentiality to produce bioactive compounds which directs its large scale cultivation. Ergosterol and Alternariol monomethyl ether were isolated from the ethyl acetate extract of the fungus, CPLE-3. <strong>Conclusion:</strong> Bioactivity and chemical screening of the endophytic fungal extracts of <em>Carica papaya</em> leaf along with the isolation of fungal secondary metabolites suggested these endophytic fungi to be possible source of bioactive leads for developing new and improved medicines.

scholarly journals Evaluation of (anti)genotoxic activities of Phyllanthus niruri L. in rat bone marrow using the micronucleus test

2013 ◽  
Vol 49 (1) ◽  
pp. 135-148 ◽  
Author(s):  
Fernando Márlisson de Queiroz ◽  
Kayo Wanderson de Oliveira Matias ◽  
Mylena Mylana Freire da Cunha ◽  
Aline Schwarz
Keyword(s):  
Bone Marrow ◽  
Body Weight ◽  
Weight Gain ◽  
Micronucleus Test ◽  
Body Weight Gain ◽  
Tap Water ◽  
Control Group ◽  
Cytotoxic Activities ◽  
Phyllanthus Niruri ◽  
Polychromatic Erythrocytes

Phyllanthus niruri L. (Euphorbiaceae), known as "quebra-pedra" (Portuguese for "stonebreaker"), is an herb used for kidney disorders. In light of its frequent use by the population, the present study aimed to investigate the genotoxic, antigenotoxic and cytotoxic activities of a standardized P. niruri extract in bone marrow rats. Three groups of 12 animals were treated daily by gavage over a period of 30 days, with 50, 150 or 250 mg/kg of P. niruri extract aqueous solution. The control group (n = 12) received tap water. At the end of treatment (day 31), groups were divided into two minor subgroups (n=6/group) and received cyclophosphamide (50 mg/kg, i.p.) or saline 0.9% (i.p.). After 24 hours, we evaluated the frequency of micronucleated polychromatic erythrocytes for each animal (MNPCE) at 1000 PCE. Cytotoxicity was evaluated with the PCE/NCE ratio (NEC = normochromatic erythrocytes). General toxicity was assessed during treatment using the parameters of body weight gain, ration and water consumption. The dry extract did not provoke changes in body weight, weight gain, ration and water intake or changes in the frequency of MNPCE or cytotoxicity in bone marrow. We propose that the P. niruri extract used here showed no genotoxic, antigenotoxic and cytotoxic activities under the experimental conditions.

scholarly journals Chemical composition and antioxidant, cytotoxic, and insecticidal potential of Valeriana alliariifolia in Turkey

2019 ◽  
Vol 70 (3) ◽  
pp. 207-218
Author(s):  
Burcu Sen-Utsukarci ◽  
Turgut Taskin ◽  
Fatih Goger ◽  
Nurhayat Tabanca ◽  
Alden S. Estep ◽  
...  
Keyword(s):  
Essential Oil ◽  
Insecticidal Activity ◽  
Folk Medicine ◽  
Added Value ◽  
Cytotoxic Activities ◽  
Dicaffeoylquinic Acid ◽  
The Difference ◽  
Ethanol Extracts ◽  
Dpph Scavenging ◽  
Bioactive Potential

AbstractValeriana is a common plant species used for various healing purposes in folk medicine since antiquity. This study investigates the phytochemical profile, antioxidant, cytotoxic, and insecticidal activity of Valeriana alliariifolia Adams, a species that has traditionally been used in Turkey. For the analyses we prepared four root extracts of V. alliariifolia Adams using hexane (HM1), chloroform (CM1), ethanol (EM1), and water (WM1) for maceration. Additionally, two extracts were also prepared from its roots by maceration separately with ethanol (EM2) and water (WM2). One sample was prepared as a water infusion (WI), according to the procedure used in Turkish traditional medicine. The 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical cation scavenging activity tests showed that ethanol extracts had the strongest antioxidant activity: EM1 (IC50 – DPPH: 17.694 µg/mL; ABTS: 23.8 µg/mL) and EM2 (IC50 – DPPH: 20 µg/mL; ABTS: 21.5 µg/mL). The hexane extract, HM1, was the most cytotoxic (IC50<10 µg/mL against HepG2 and HUVEC) and EM2 strongly cytotoxic (IC50<10 µg/mL against HepG2 and IC50: 11.96 µg/mL against HUVEC). The extracts with demonstrated cytotoxic activities were further examined to check their insecticidal activity against adult female mosquito Aedes aegypti and first instar Ae. aegypti larvae. HM1 was the most effective (90±10 %), which was consistent with its cytotoxic activity. Because of the high antioxidant, cytotoxic, and insecticidal activities, we ran phytochemical analyses of the HM1, EM1, and EM2 extracts with GC-MS (for HM1) and LC-MS/MS (for EM1 and EM2). We also analysed the composition of the essential oil obtained from V. alliariifolia roots by micro-distillation in order to compare its content with HM1, which contains volatile compounds. Phytochemical analyses revealed that the major compound in HM1 was isovaleric acid (16 %) and in the essential oil 1,8-cineole (2.9 %). EM1 and EM2 contained 5-O-caffeoylquinic acid (chlorogenic acid), verbascoside (acteoside), and 3,5-dicaffeoylquinic acid as major components. In the light of our findings and available literature, we can conclude that V. alliariifolia has a good bioactive potential that could be used for different purposes, including the development of new agents for the treatment of various diseases. The difference in the content between the essential oil and HM1 was remarkable. It suggests that the variability observed in the activity of the samples was a result of composition and that, therefore, the aim of treatment should dictate which type of preparation is to be selected. An added value of our study is that it determined verbascoside and methylquercetin rutinoside for the first time in the Valeriana extracts.

scholarly journals In Vitro Antioxidant, Cytotoxic Activities, and Phenolic Profile of Senecio glaucus from Saudi Arabia

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Ali S. Alqahtani ◽  
Rashed N. Herqash ◽  
Omar M. Noman ◽  
Fahd A. Nasr ◽  
Nouf Alyhya ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Side Effects ◽  
Cytotoxic Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Hplc Method ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Chloroform Fraction ◽  
Promising Source

Current treatments for complex diseases have remarkable side effects that negatively impact patients’ quality of life. Thus, natural compounds with fewer side effects represent a promising source for safe drugs. The genus Senecio is widely used in folk medicine due to its various pharmacological properties. In the present study, the total phenolic content of Senecio glaucus, which is grown in Saudi Arabia, was assessed using the Folin-Ciocalteau colorimetric method. Scavenging DPPH and ABTS assays were utilized to determine the antioxidant properties of S. glaucus fractions, and MTT assay was used to screen the cytotoxic activity of S. glaucus against various cancer cells. In addition, HPLC-UV was utilized to detect the presence of two phenolic acids, namely, vanillic acid (VA) and gallic acid (GA). Among all fractions tested, S. glaucus chloroform fraction (SGCF) yielded the highest value (125.3 mg·GA/g) in terms of total phenolic content. SGCF also exhibited the highest scavenging activities (76.7 and 74.1%) on both DPPH and ABTS assays, respectively. Similarly, SGCF also possessed the most potent cytotoxic activity against the MCF-7 cell line, with an IC50 value of 41.8 μg/ml. The validated HPLC method confirmed the presence of VA (4.8 μg/mg DW) and GA (3.9 μg/mg DW) in SGCF. Overall, our data show that S. glaucus had antioxidant and cytotoxic properties. A developed validated HPLC method which could be helpful for quantifying phenolic compounds in S. glaucus was established.

scholarly journals Major Constituents of the Predominant Endophytic Fungi from the Nigerian Plants Bryophyllum Pinnatum, Morinda Lucida and Jathropha Gossypiifolia

2008 ◽  
Vol 3 (8) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Abimbola A. Sowemimo ◽  
RuAngelie Edrada-Ebel ◽  
Rainer Ebel ◽  
Peter Proksch ◽  
Olanrewaju R. Omobuwajo ◽  
...  
Keyword(s):  
Endophytic Fungi ◽  
Cytochalasin D ◽  
Lymphoma Cell Line ◽  
Strong Activity ◽  
Chemical Screening ◽  
Diaporthe Phaseolorum ◽  
Hydroxyphenylacetic Acid ◽  
Jatropha Gossypiifolia ◽  
Mouse Lymphoma ◽  
Bryophyllum Pinnatum

In a chemical screening of fungal strains endophytic to Nigerian plants, Diaporthe phaseolorum, the endophyte of Bryophyllum pinnatum, gave cytochalasin D and (3 R,4 R)-3,4,5-trihydroxy-1-tetralone, Neurospora discreta from Morinda lucida yielded multiforisin I and 4-hydroxyphenylacetic acid, while Aspergillus japonicus from Jatropha gossypiifolia gave (+)-eupenoxide and microsphaerone C, a new 4-pyridone derivative. When tested for cytotoxic activity, cytochalasin D displayed strong activity towards the mouse lymphoma cell line L5178Y, while the remaining compounds, except 4-hydroxyphenylacetic acid, were found to be moderately active.

Antibacterial Activity of Traditional Medicine Scurrula atropurpurea (BL) DANS and their Endophytic Fungi

2020 ◽  
Vol 840 ◽  
pp. 205-213
Author(s):  
Elfita Elfita ◽  
Muharni Muharni ◽  
Mardiyanto Mardiyanto ◽  
Fitrya Fitrya ◽  
Feti Fera ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Bacterial Infections ◽  
Salmonella Typhi ◽  
Spectroscopy Analysis ◽  
Chromatography Method ◽  
Antibacterial Compounds ◽  
Activity Test ◽  
Antibacterial Compound ◽  
Promising Source

Scurrula atropurpurea known as benalu is a medicinal plant that has been used for the treatment of various diseases such as antibacterial. Plants with ethnomedicine history use to cure pathogenic bacterial infections and their endophytic fungi is a promising source of antibacterial compounds. This study aimed to compared the antibacterial activity of S. atropurpurea leaves and their endophytic fungi. The secondary metabolites were isolated from the leaves of S. atropurpurea and their endophytic fungi by the chromatography method. The antibacterial activity test was carried out by Kirby Bauer method against Salmonella typhi (IPCCCB.11.669) and Escherichia coli (ATCC 25922) as Gram (-) and Bacillus subtilis (ATCC 6633) and Staphylococcus aureus (ATCC 25923) as Gram (+). The antibacterial compound from S. atropurpurea was determined by spectroscopy analysis as Quercetin-3-O-α-L-Rhamnopyranoside, while the antibacterial compound from endophytic fungi (strain BB1) as a lactone. Phylogenetic tree of strain BB1 has the highest homology with Neopestalotiopsis surinamensis strain CBS 450.74.

scholarly journals Biodegradation of Polyester Polyurethane by Endophytic Fungi

2011 ◽  
Vol 77 (17) ◽  
pp. 6076-6084 ◽  
Author(s):  
Jonathan R. Russell ◽  
Jeffrey Huang ◽  
Pria Anand ◽  
Kaury Kucera ◽  
Amanda G. Sandoval ◽  
...  
Keyword(s):  
Carbon Source ◽  
Endophytic Fungi ◽  
Sole Carbon Source ◽  
Anaerobic Growth ◽  
Serine Hydrolase ◽  
Microbial World ◽  
Content Type ◽  
Metabolic Properties ◽  
Liquid Suspensions ◽  
Promising Source

ABSTRACTBioremediation is an important approach to waste reduction that relies on biological processes to break down a variety of pollutants. This is made possible by the vast metabolic diversity of the microbial world. To explore this diversity for the breakdown of plastic, we screened several dozen endophytic fungi for their ability to degrade the synthetic polymer polyester polyurethane (PUR). Several organisms demonstrated the ability to efficiently degrade PUR in both solid and liquid suspensions. Particularly robust activity was observed among several isolates in the genusPestalotiopsis, although it was not a universal feature of this genus. TwoPestalotiopsis microsporaisolates were uniquely able to grow on PUR as the sole carbon source under both aerobic and anaerobic conditions. Molecular characterization of this activity suggests that a serine hydrolase is responsible for degradation of PUR. The broad distribution of activity observed and the unprecedented case of anaerobic growth using PUR as the sole carbon source suggest that endophytes are a promising source of biodiversity from which to screen for metabolic properties useful for bioremediation.

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