The impact of short-term refrigeration of human lipoaspirate on adipose-derived stem cells and adipocytes

2015 ◽  
Vol 68 (1) ◽  
pp. 137-139 ◽  
Author(s):  
Wei Z. Wang ◽  
Xin-Hua Fang ◽  
Shelley J. Williams ◽  
Linda L. Stephenson ◽  
Richard C. Baynosa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Short Term ◽  
The Impact

scholarly journals In Vitro Studies on Nanoporous, Nanotubular and Nanosponge-Like Titania Coatings, with the Use of Adipose-Derived Stem Cells

Materials ◽  
2020 ◽  
Vol 13 (7) ◽  
pp. 1574 ◽  
Author(s):  
Michalina Ehlert ◽  
Aleksandra Radtke ◽  
Tomasz Jędrzejewski ◽  
Katarzyna Roszek ◽  
Michał Bartmański ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Line ◽  
Osteogenic Potential ◽  
Biological Research ◽  
Adipose Derived Stem Cells ◽  
Differentiation Potential ◽  
Nanoporous Surface ◽  
Titania Coatings ◽  
The Impact

In vitro biological research on a group of amorphous titania coatings of different nanoarchitectures (nanoporous, nanotubular, and nanosponge-like) produced on the surface of Ti6Al4V alloy samples have been carried out, aimed at assessing their ability to interact with adipose-derived mesenchymal stem cells (ADSCs) and affect their activity. The attention has been drawn to the influence of surface coating architecture and its physicochemical properties on the ADSCs proliferation. Moreover, in vitro co-cultures: (1) fibroblasts cell line L929/ADSCs and (2) osteoblasts cell line MG-63/ADSCs on nanoporous, nanotubular and nanosponge-like TiO2 coatings have been studied. This allowed for evaluating the impact of the surface properties, especially roughness and wettability, on the creation of the beneficial microenvironment for co-cultures and/or enhancing differentiation potential of stem cells. Obtained results showed that the nanoporous surface is favorable for ADSCs, has great biointegrative properties, and supports the growth of co-cultures with MG-63 osteoblasts and L929 fibroblasts. Additionally, the number of osteoblasts seeded and cultured with ADSCs on TNT5 surface raised after 72-h culture almost twice when compared with the unmodified scaffold and by 30% when compared with MG-63 cells growing alone. The alkaline phosphatase activity of MG-63 osteoblasts co-cultured with ADSCs increased, that indirectly confirmed our assumptions that TNT-modified scaffolds create the osteogenic niche and enhance osteogenic potential of ADSCs.

scholarly journals The Impact of Lidocaine on Adipose-Derived Stem Cells in Human Adipose Tissue Harvested by Liposuction and Used for Lipotransfer

2020 ◽  
Vol 21 (8) ◽  
pp. 2869
Author(s):  
Felix Grambow ◽  
Rico Rutkowski ◽  
Fred Podmelle ◽  
Katrin Schmoeckel ◽  
Florian Siegerist ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Small Sample Size ◽  
Subcutaneous Fat ◽  
Stromal Vascular Fraction ◽  
Small Sample ◽  
Relative Distribution ◽  
Adipose Derived Stem Cells ◽  
Female Patients ◽  
The Impact

The local anesthetic lidocaine, which has been used extensively during liposuction, has been reported to have cytotoxic effects and therefore would be unsuitable for use in autologous lipotransfer. We evaluated the effect of lidocaine on the distribution, number, and viability of adipose-derived stem cells (ASCs), preadipocytes, mature adipocytes, and leukocytes in the fatty and fluid portion of the lipoaspirate using antibody staining and flow cytometry analyses. Adipose tissue was harvested from 11 female patients who underwent liposuction. Abdominal subcutaneous fat tissue was infiltrated with tumescent local anesthesia, containing lidocaine on the left and lacking lidocaine on the right side of the abdomen, and harvested subsequently. Lidocaine had no influence on the relative distribution, cell number, or viability of ASCs, preadipocytes, mature adipocytes, or leukocytes in the stromal-vascular fraction. Assessing the fatty and fluid portions of the lipoaspirate, the fatty portions contained significantly more ASCs (p < 0.05), stem cells expressing the preadipocyte marker Pref-1 (p < 0.01 w/lidocaine, p < 0.05 w/o lidocaine), and mature adipocytes (p < 0.05 w/lidocaine, p < 0.01 w/o lidocaine) than the fluid portions. Only the fatty portion should be used for transplantation. This study found no evidence that would contraindicate the use of lidocaine in lipotransfer. Limitations of the study include the small sample size and the inclusion of only female patients.

scholarly journals The Impact of Short-Term Dietary Restriction on Stem Cell Function

2021 ◽  
Vol 5 (Supplement_1) ◽  
pp. 116-117
Author(s):  
Archana Unnikrishnan
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Dietary Restriction ◽  
Cell Function ◽  
Aging Effects ◽  
Short Term ◽  
Regenerative Capacity ◽  
Short Period ◽  
The Impact ◽  
Old Mice

Abstract Stem cells play a critical role in the maintenance of tissue function and their proliferative/regenerative capacity is essential to this role. Because stem cells persist over the lifespan of an animal, they are susceptible to gradual accumulation of age-associated damage, resulting in the loss of regenerative function that can impair organ function. Understanding the mechanism(s) that regulates stem cell function is essential for retarding the aging process, and stem cells are attractive targets for aging interventions. Dietary restriction (DR), the most robust anti-aging intervention to-date, has been shown to enhance the activity and integrity of stem cells in a variety of tissues (e.g., muscle, bone marrow, and intestine), and it is believed that effect of DR on stem cells plays an important role in the anti-aging action of DR. For example, DR has been shown to preserve and increase the number of intestinal stem cells (ISCs) and enhance their regenerative capacity in young animals. Data from my lab shows that ISCs from old mice have limited proliferation activity and form few if any organoids in vitro (a surrogate for a fully functional crypt) and that ISCs isolated from old mice on life-long DR show an improved ability to form organoids. While it is well accepted that life-long DR increases lifespan and has anti-aging effects an important aspect of DR that has been largely overlooked is that DR implemented only for a short time early in life can increase lifespan of rodents even when rodents are fed ad libitum the remainder of their life. In line with this, we recently found that ISCs from old mice fed DR for only a short-period resulted in a dramatic increase in ability of the ISCs to form organoids. This is the first evidence that short-term DR administrated late in life can rescue the loss in ISC function that occurs with age.

Short-term evaluation of electromagnetic field pretreatment of adipose-derived stem cells to improve bone healing

2012 ◽  
Vol 9 (10) ◽  
pp. 1161-1171 ◽  
Author(s):  
Kyung Shin Kang ◽  
Jung Min Hong ◽  
Young-Joon Seol ◽  
Jong-Won Rhie ◽  
Young Hun Jeong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Electromagnetic Field ◽  
Bone Healing ◽  
Adipose Derived Stem Cells ◽  
Short Term ◽  
Short Term Evaluation ◽  
Term Evaluation

scholarly journals The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin Aging

2020 ◽  
Vol 2020 ◽  
pp. 1-19
Author(s):  
Feng Qin ◽  
Jiuzuo Huang ◽  
Wenchao Zhang ◽  
Mingzi Zhang ◽  
Zhenjiang Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Adipose Derived Stem Cells ◽  
Paracrine Effects ◽  
Skin Photoaging ◽  
Uvb Exposure ◽  
Underlying Mechanisms ◽  
The Impact

Background. Human dermal fibroblasts (HDFs) are the primary cells in skin and are associated with UVB-induced skin photoaging. Adipose-derived stem cells (ASCs) have been proposed as a treatment for skin aging. The goal of this study was to investigate paracrine mechanisms by which ASCs repair HDFs damage from UVB exposure. Methods. ASCs were cocultured with UVB-irradiated and nonirradiated HDFs. We compared HDF senescence, proliferation, migration, oxidative stress, and cytokine expression. In a nude mouse UVB-induced photoaging model, ASCs were injected subcutaneously, and skin samples were collected weekly between postoperative weeks 3 through 7. Histological analysis, PCR, ELISA, and immunohistochemistry were used to analyze the effect of ASCs. Results. Compared with UVB-irradiated HDFs, nonirradiated HDFs showed higher proliferation and migration, reduced apoptosis, and fewer senescent cells when cocultured with ASCs. The expression of extracellular matrix-related cytokines was also regulated by ASCs. In addition, ASCs effectively reversed UVB-induced skin photoaging in vivo. We propose that ASCs more robustly coordinate healthy HDFs than UVB-damaged HDFs to repair aging skin. Conclusions. ASCs improved the function of both UVB-damaged and healthy HDFs through paracrine effects. However, the impact of ASCs on healthy HDFs was greater than UVB-damaged HDFs. These findings help to elucidate the underlying mechanisms of the skin rejuvenation effect of ASCs.

scholarly journals Canine Adipose Derived Mesenchymal Stem Cells Transcriptome Composition Alterations: A Step towards Standardizing Therapeutic

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Nina Krešić ◽  
Ivana Šimić ◽  
Ivana Lojkić ◽  
Tomislav Bedeković
Keyword(s):  
Stem Cells ◽  
Therapeutic Potential ◽  
Ex Vivo ◽  
Adipose Derived Stem Cells ◽  
Morphological Alterations ◽  
Undifferentiated State ◽  
Passage Number ◽  
The Impact ◽  
First Time ◽  
Morphology Characteristics

Although canine adipose derived stem cells (cASCs) morphology characteristics and differentiation ability are well documented, transcriptome alterations of undifferentiated cASCs during ex vivo cultivation remain unknown. Here we demonstrate, for the first time, the transcriptome composition of isolated cASCs in undifferentiated state originating from six donors. Transcriptome changes were monitored during ex vivo cultivation between passage 3 (P3) and P5, which are mostly used in therapy. Influence of donors’ age in given passage number on transcriptome composition was also investigated. Cultivation from P3 to P5 resulted in 16 differentially expressed genes with cooverexpression of pluripotency and self-renewal transcription factors genes SOX2 and POU5F1 dominant in old donors’ cells. Furthermore, cASCs demonstrated upregulation of IL-6 in young and old donors’ cells. In addition, ex vivo cultivation of cASCs revealed well-known morphological alterations accompanied with decrease in expression of CD90 and CD44 markers in P4 and higher monitored by flow cytometry and successful osteo- and chondrodifferentiation but inefficient adipodifferentiation in P3. Our results revealed the impact of ex vivo cultivation on nature of cells. Correlation of transcriptome changes with secretome composition is needed and its further impact on therapeutic potential of cASCs remains to be evaluated in clinical trials.

scholarly journals Short-term mechanical stretch fails to differentiate human adipose-derived stem cells into cardiovascular cell phenotypes

2014 ◽  
Vol 13 (1) ◽  
pp. 54 ◽  
Author(s):  
Thais Girão-Silva ◽  
Vinicius Bassaneze ◽  
Luciene Cristina Campos ◽  
Valerio Barauna ◽  
Luis Alberto Dallan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mechanical Stretch ◽  
Adipose Derived Stem Cells ◽  
Short Term ◽  
Cell Phenotypes

scholarly journals Effect of Pig-Adipose-Derived Stem Cells’ Conditioned Media on Skin Wound-Healing Characteristics In Vitro

2021 ◽  
Vol 22 (11) ◽  
pp. 5469
Author(s):  
Joanna Wiśniewska ◽  
Magda Słyszewska ◽  
Karolina Stałanowska ◽  
Katarzyna Walendzik ◽  
Marta Kopcewicz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Three Dimensional ◽  
Dermal Fibroblasts ◽  
Conditioned Media ◽  
Adipose Derived Stem Cells ◽  
Skin Wound Healing ◽  
Cell Functions ◽  
The Impact

The primary mechanism by which adipose-derived stem cells (ASCs) exert their reparative or regenerative potential relies predominantly on paracrine action. Secretory abilities of ASCs have been found to be amplified by hypoxia pre-conditioning. This study investigates the impact of hypoxia (1% O2) on the secretome composition of pig ASCs (pASCs) and explores the effect of pASCs’ conditioned media (CM) on skin cell functions in vitro and the expression of markers attributed to wound healing. Exposure of pASCs to hypoxia increased levels of vascular endothelial growth factor (VEGF) in CM-Hyp compared to CM collected from the cells cultured in normoxia (CM-Nor). CM-Hyp promoted the migratory ability of pig keratinocytes (pKERs) and delayed migration of pig dermal fibroblasts (pDFs). Exposure of pKERs to either CM-Nor or CM-Hyp decreased the levels of pro-fibrotic indicators WNT10A and WNT11. Furthermore, CM-Hyp enhanced the expression of KRT14, the marker of the basal epidermis layer. In contrast, CM-Nor showed a stronger effect on pDFs manifested by increases in TGFB1, COL1A1, COL3A1, and FN1 mRNA expression. The formation of three-dimensional endothelial cell networks was improved in the presence of CM-Hyp. Overall, our results demonstrate that the paracrine activity of pASCs affects skin cells, and this property might be used to modulate wound healing.

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