Novel resin tissue array system reduces sample preparation time, labour and reagent costs in bone tissue histology

Bone ◽  
2021 ◽  
Vol 153 ◽  
pp. 116155
Author(s):  
Jiongyu Ren ◽  
Naomi C. Paxton ◽  
Joshua Hammond ◽  
Siamak Saifzadeh ◽  
Roland Steck ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Bone Tissue ◽  
Tissue Array ◽  
Preparation Time ◽  
Tissue Histology

Doping and Alloying Trends in New Thermoelectric Materials

2001 ◽  
Vol 691 ◽  
Author(s):  
Sim Loo ◽  
Sangeeta Lal ◽  
Theodora Kyratsi ◽  
Duck-Young Chung ◽  
Kuei-Fang Hsu ◽  
...  
Keyword(s):  
Thermal Conductivity ◽  
Electrical Conductivity ◽  
Sample Preparation ◽  
Measurement System ◽  
Thermoelectric Materials ◽  
Bulk Materials ◽  
Preparation Time ◽  
Short Sample ◽  
Hall Effect Measurements ◽  
Computer Controlled

ABSTRACTNew thermoelectric bulk materials such as CsBi4Te6 have shown superior properties to traditional materials, however, optimal performance requires continuing investigations of doping and alloying trends. A recently modified high throughput measurement system is presented for doping and alloying investigations in several new thermoelectric materials. The modification includes a four-probe configuration for more accurate measurements while maintaining a relatively short sample preparation time. The system is fully computer controlled and provides flexible contacts to accommodate various sample dimensions. Optimal compositions are then identified for further investigations in thermoelectric prototype modules. The most promising materials will be further characterized for electrical conductivity, thermoelectric power, thermal conductivity, and Hall effect measurements as a function of temperature.

Semi-automated liquid–liquid back-extraction in a 96-well format to decrease sample preparation time for the determination of dextromethorphan and dextrorphan in human plasma

2002 ◽  
Vol 772 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Renee D. Bolden ◽  
Steven H. Hoke II ◽  
Thomas H. Eichhold ◽  
David L. McCauley-Myers ◽  
Kenneth R. Wehmeyer
Keyword(s):  
Sample Preparation ◽  
Human Plasma ◽  
Back Extraction ◽  
Preparation Time

A Technique for Preparing “Two in One” Cross-Sectional TEM Specimens

1990 ◽  
Vol 199 ◽  
Author(s):  
Guang-Hwa M. Ma ◽  
Sopa Chevacharoenkul
Keyword(s):  
Sample Preparation ◽  
The Other ◽  
Specimen Preparation ◽  
Preparation Technique ◽  
Preparation Time ◽  
Cross Sectional ◽  
Sample Preparation Technique ◽  
Two Samples ◽  
Junction Formation ◽  
Suitable Marker

ABSTRACTA modified “two-in-one” cross-sectional TEM sample preparation technique is described. By coating a thin layer of “marker” to distinguish one sample from the other, two samples could be simultaneously prepared in one TEM cross-sectional specimen. Therefore, the specimen preparation time is reduced by nearly one half. The coating can be done in an existing ion-mill. Criteria for choosing a suitable marker as well as tips on getting good quality specimens are described. An example of applying this technique to a processing-microstructure study of an ultra-shallow junction formation in silicon is demonstrated.

Rapid Determination of Aflatoxins in Raw Peanuts by Liquid Chromatography with Postcolumn Iodination and Modified Minicolumn Cleanup

1988 ◽  
Vol 71 (1) ◽  
pp. 43-47 ◽  
Author(s):  
Joe W Dorner ◽  
Richard J Cole
Keyword(s):  
Sample Preparation ◽  
Rapid Determination ◽  
Peanut Meal ◽  
Preparation Time ◽  
Coefficients Of Variation ◽  
Phase Liquid ◽  
Water Saturated ◽  
Liquid Chromatographic ◽  
Postcolumn Derivatization

Abstract A method is described for rapid cleanup followed by reverse-phase liquid chromatographic (LC) quantitation of aflatoxins in raw peanuts. A modified minicolumn cleanup is used for sample preparation, and a preliminary estimation of aflatoxin content by minicolumn can be made so that highly contaminated samples can be diluted before LC analysis. The use of the simple, quick minicolumn cleanup eliminates the need for further column or cartridge cleanup, thus greatly reducing sample preparation time. Sensitive quantitation is achieved using a phenyl column, a mobile phase of water-tetrahydrofuran (80 + 20, v/v), and postcolumn derivatization with water-saturated iodine followed by fluorescence detection. The recoveries of aflatoxins B1, B2, G1, and G2 from peanut meal spiked at 3 levels ranged from 71.7 to 88.3% (average 80%) with coefficients of variation from 2.7 to 10.4%.

scholarly journals Automation of Bone Tissue Histology

2019 ◽  
Vol 7 ◽  
Author(s):  
Viktoriya V. Yaikova ◽  
Oleg V. Gerasimov ◽  
Artur O. Fedyanin ◽  
Mikhail A. Zaytsev ◽  
Maxim E. Baltin ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Histology

A Modified Babcock Procedure for Rapid Determination of Fat in Meats Using Dimethyl Sulfoxide

1966 ◽  
Vol 49 (6) ◽  
pp. 1225-1229
Author(s):  
Francis Whalen
Keyword(s):  
Phase Separation ◽  
Sample Preparation ◽  
Dimethyl Sulfoxide ◽  
Rapid Method ◽  
Process Monitoring ◽  
Rapid Determination ◽  
Meat Products ◽  
Special Equipment ◽  
Preparation Time

Abstract A rapid method of determining fat in a wide variety of meat products has been developed. The meat is digested with hot HO and the products of the hydrolysis are dissolved in dimethyl sulfoxide; neutral triglycerides are insoluble in this solvent. The solubility of starches, sugars, and the usual curing inorganics in dimethyl sulfoxide and its miscibility with water prevent interference by these substances. Digestions are rapid, and phase separation and demarcation are excellent. One analysis (exclusive of sample preparation time) takes approximately 10 minutes. Only two reagents must be purchased, and no special equipment other than the usual Babcock apparatus is needed. Results by the sulfoxide procedure approximate results by the Mojonnier method. Its speed and simplicity should make it valuable for process monitoring and formulation adjustment in the manufacture of meat products.

Analytical Methodology for Sample Preparation, Detection, Quantitation, and Confirmation of N-Nitrosamines in Foods

1981 ◽  
Vol 64 (5) ◽  
pp. 1037-1054
Author(s):  
Joseph H Hotchkiss
Keyword(s):  
Sample Preparation ◽  
Review Paper ◽  
Complex Mixtures ◽  
Nitroso Compounds ◽  
Preparation Time ◽  
Analytical Methodology ◽  
Chromatographic Techniques ◽  
The Past ◽  
Selective Detectors ◽  
Further Development

Abstract Significant advances have been made over the past decade in methodology for the analysis of foods and other samples for volatile N-nitrosamines. Procedures for the isolation, cleanup, and concentration of N-nitrosamines were developed and applied to a broad range of food types. Several chromatographic techniques and systems have also been developed which are capable of resolving AT-nitrosamines in complex mixtures in a single run, and the use of highly selective detectors has decreased sample preparation time while increasing sensitivity and precision. Unequivocal confirmation of N-nitrosamines in foods can now be achieved by mass spectrometry at low to sub-μg/kg levels. However, further development of methodology for nonvolatile N-nitroso compounds is needed. This review paper discusses these and other topics related to the analysis of foods for N-nitrosamines.

scholarly journals Sample preparation of bone tissue for MALDI-MSI for forensic and (pre)clinical applications

2020 ◽  
Author(s):  
Michiel Vandenbosch ◽  
Sylvia P. Nauta ◽  
Anastasiya Svirkova ◽  
Martijn Poeze ◽  
Ron M.A. Heeren ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Bone Tissue ◽  
Limit Of Detection ◽  
Ionization Mass ◽  
The Past ◽  
Human Bones ◽  
Sectioning Method ◽  
Clinical Investigations ◽  
Insulating Properties ◽  
Positive Ion

AbstractIn the past decades, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been applied to a broad range of biological samples, e.g., forensics and preclinical samples. The use of MALDI-MSI for the analysis of bone tissue has been limited due to the insulating properties of the material but more importantly the absence of a proper sample preparation protocol for undecalcified bone tissue. Undecalcified sections are preferred to retain sample integrity as much as possible or to study the tissue-bone bio interface in particular. Here, we optimized the sample preparation protocol of undecalcified bone samples, aimed at both targeted and untargeted applications for forensic and preclinical applications, respectively. Different concentrations of gelatin and carboxymethyl cellulose (CMC) were tested as embedding materials. The composition of 20% gelatin and 7.5% CMC showed to support the tissue best while sectioning. Bone tissue has to be sectioned with a tungsten carbide knife in a longitudinal fashion, while the sections need to be supported with double-sided tapes to maintain the morphology of the tissue. The developed sectioning method was shown to be applicable on rat and mouse as well as human bone samples. Targeted (methadone and EDDP) as well as untargeted (unknown lipids) detection was demonstrated. DHB proved to be the most suitable matrix for the detection of methadone and EDDP in positive ion mode. The limit of detection (LOD) is estimated to approximately 50 pg/spot on bone tissue. The protocol was successfully applied to detect the presence of methadone and EDDP in a dosed rat femur and a dosed human clavicle. The best matrices for the untargeted detection of unknown lipids in mouse hind legs in positive ion mode were CHCA and DHB based on the number of tissue-specific peaks and signal-to-noise ratios. The developed and optimized sample preparation method, applicable on animal and human bones, opens the door for future forensic and (pre)clinical investigations.

A Novel System for Sedimentary Organic Carbon Isotope Sample Preparation

2013 ◽  
Vol 333-335 ◽  
pp. 1899-1902
Author(s):  
Zheng Zhai ◽  
Qing Liu ◽  
Xing You Xu ◽  
Ju Yuan Li ◽  
Zheng Li ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Sampling Device ◽  
Preparation Time ◽  
Organic Carbon Isotope ◽  
Low Degree ◽  
Sedimentary Organic Carbon ◽  
Standard Operating ◽  
Automatic Sampling ◽  
New Type ◽  
High Degree

A new type of isotope preparation system was designed with excellent accuracy, high degree automation and high security. In view of the existing equipment with automation low degree automation, poor safety, much long preparation time and low precision of the sample preparation problems, a series of improvements were introduced, such as the automatic sampling device, contact valve, vacuum auxiliary system, gas meter and digital dashboards. It was found that the instrument finely designed had the characteristics such as high accuracy, automation and security. Combined with the sample detection device, the preparated sample had excellent detection results. Meanwhile, a set of standard operating procedures had also been formed. Furthermore, the sample preparation process was briefly discussed.

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