scholarly journals Quantitative Analysis of Interactive Behavior of Mitochondria and Lysosomes using Structured Illumination Microscopy

2019 ◽  
Vol 116 (3) ◽  
pp. 267a ◽  
Author(s):  
Qixin Chen ◽  
Xintian Shao ◽  
Peixue Ling ◽  
Jiajie Diao
Keyword(s):  
Quantitative Analysis ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Interactive Behavior

scholarly journals Quantitative analysis of interactive behavior of mitochondria and lysosomes using structured illumination microscopy

Biomaterials ◽  
2020 ◽  
Vol 250 ◽  
pp. 120059 ◽  
Author(s):  
Qixin Chen ◽  
Xintian Shao ◽  
Mingang Hao ◽  
Hongbao Fang ◽  
Ruilin Guan ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Interactive Behavior

scholarly journals Quantitative Analysis of Interactive Behavior of Mitochondria and Lysosomes Using Structured Illumination Microscopy

2018 ◽  
Author(s):  
Qixin Chen ◽  
Xintian Shao ◽  
Mingang Hao ◽  
Zhiqi Tian ◽  
Chenran Wang ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Optical Microscopy ◽  
Spatial Resolution ◽  
Cell Biology ◽  
Super Resolution ◽  
Cellular Level ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Interactive Behavior ◽  
Molecular Machinery

ABSTRACTSuper-resolution optical microscopy has extended the spatial resolution of cell biology from the cellular level to the nanoscale, enabling the observation of the interactive behavior of single mitochondria and lysosomes. Quantitative parametrization of interaction between mitochondria and lysosomes under super-resolution optical microscopy, however, is currently unavailable, which has severely limited our understanding of the molecular machinery underlying mitochondrial functionality. Here, we introduce an M-value to quantitatively investigate mitochondria and lysosome contact (MLC) and mitophagy under structured illumination microscopy. We found that the M-value for an MLC is typically less than 0.4, whereas in mitophagy it ranges from 0.5 to 1.0. This system permits further investigation of the detailed molecular mechanism governing the interactive behavior of mitochondria and lysosomes.

Structured illumination microscopy and correlative microscopy to study autophagy

Methods ◽  
2015 ◽  
Vol 75 ◽  
pp. 61-68 ◽  
Author(s):  
Laure-Anne Ligeon ◽  
Nicolas Barois ◽  
Elisabeth Werkmeister ◽  
Antonino Bongiovanni ◽  
Frank Lafont
Keyword(s):  
Structured Illumination ◽  
Correlative Microscopy ◽  
Structured Illumination Microscopy

Cost-Effective Live Cell Structured Illumination Microscopy with Video-Rate Imaging

ACS Photonics ◽  
2021 ◽  
Author(s):  
Alice Sandmeyer ◽  
Mario Lachetta ◽  
Hauke Sandmeyer ◽  
Wolfgang Hübner ◽  
Thomas Huser ◽  
...  
Keyword(s):  
Cost Effective ◽  
Live Cell ◽  
Structured Illumination ◽  
Structured Illumination Microscopy

scholarly journals Growth factor dependent changes in nanoscale architecture of focal adhesions

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Karin Legerstee ◽  
Tsion E. Abraham ◽  
Wiggert A. van Cappellen ◽  
Alex L. Nigg ◽  
Johan A. Slotman ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Intermediate Layer ◽  
Cell Movement ◽  
Focal Adhesions ◽  
Bottom Layer ◽  
Vertical Axis ◽  
Longitudinal Distribution ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Mediate Cell

AbstractFocal adhesions (FAs) are flat elongated structures that mediate cell migration and link the cytoskeleton to the extracellular matrix. Along the vertical axis FAs were shown to be composed of three layers. We used structured illumination microscopy to examine the longitudinal distribution of four hallmark FA proteins, which we also used as markers for these layers. At the FA ends pointing towards the adherent membrane edge (heads), bottom layer protein paxillin protruded, while at the opposite ends (tails) intermediate layer protein vinculin and top layer proteins zyxin and VASP extended further. At the tail tips, only intermediate layer protein vinculin protruded. Importantly, head and tail compositions were altered during HGF-induced scattering with paxillin heads being shorter and zyxin tails longer. Additionally, FAs at protruding or retracting membrane edges had longer paxillin heads than FAs at static edges. These data suggest that redistribution of FA-proteins with respect to each other along FAs is involved in cell movement.

scholarly journals Film Thickness-Profile Measurement Using Iterative Peak Separation Structured Illumination Microscopy

2021 ◽  
Vol 11 (7) ◽  
pp. 3023
Author(s):  
Kejun Yang ◽  
Chenhaolei Han ◽  
Jinhua Feng ◽  
Yan Tang ◽  
Zhongye Xie ◽  
...  
Keyword(s):  
Modulation Depth ◽  
Thickness Distribution ◽  
Detection Threshold ◽  
Profile Measurement ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Separation Algorithm ◽  
Peak Separation ◽  
Lower Detection ◽  
Depth Response

The surface and thickness distribution measurement for transparent film is of interest for electronics and packaging materials. Structured illumination microscopy (SIM) is a prospective technique for measuring film due to its high accuracy and efficiency. However, when the distance between adjacent layers becomes close, the peaks of the modulation depth response (MDR) start to overlap and interfere with the peak extraction, which restricts SIM development in the field of film measurement. In this paper, an iterative peak separation algorithm is creatively applied in the SIM-based technique, providing a precise peak identification even as the MDR peaks overlap and bend into one. Compared with the traditional method, the proposed method has a lower detection threshold for thickness. The experiments and theoretical analysis are elaborated to demonstrate the feasibility of the mentioned method.

scholarly journals Two-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM)

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Liliana Barbieri ◽  
Huw Colin-York ◽  
Kseniya Korobchevskaya ◽  
Di Li ◽  
Deanna L. Wolfson ◽  
...  
Keyword(s):  
Resolution Enhancement ◽  
Traction Force ◽  
Super Resolution ◽  
Traction Force Microscopy ◽  
Two Dimensional ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Force Microscopy ◽  
Health And Disease ◽  
Spatio Temporal

AbstractQuantifying small, rapidly evolving forces generated by cells is a major challenge for the understanding of biomechanics and mechanobiology in health and disease. Traction force microscopy remains one of the most broadly applied force probing technologies but typically restricts itself to slow events over seconds and micron-scale displacements. Here, we improve >2-fold spatially and >10-fold temporally the resolution of planar cellular force probing compared to its related conventional modalities by combining fast two-dimensional total internal reflection fluorescence super-resolution structured illumination microscopy and traction force microscopy. This live-cell 2D TIRF-SIM-TFM methodology offers a combination of spatio-temporal resolution enhancement relevant to forces on the nano- and sub-second scales, opening up new aspects of mechanobiology to analysis.

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