Identification of phenolic compounds in the buds of Algerian Pistacia atlantica desf. Subsp. atlantica by antioxidant activity guided fractionation

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Fethi Toul ◽  
Sonia Moussouni ◽  
Nacera Ghembaza ◽  
Amel Zitouni ◽  
Amina Djendar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Spectroscopic Techniques ◽  
Active Compounds ◽  
Pistacia Atlantica ◽  
Tlc Plates ◽  
Dpph Scavenging

Abstract Objectives A bio-guided selection and identification of the most active compounds obtained from Algerian Pistacia atlantica desf. Subsp. atlantica. Methods An antioxidant activity guided fractionation was performed on buds’ extract using extensive chromatographic and spectroscopic techniques. The antioxidant potentials of isolated compounds and other unpurified fractions were evaluated against DPPH radicals using TLC plates and test tubes. Results The results showed that all isolated compounds and fractions exhibited eminent DPPH scavenging potential. Two coumarins (7-ethoxycoumarin and 7-hydroxy-5-methoxycoumarin) and two flavonoids (3′,5,7-Trihydroxy-4′-methoxyflavanone and 5,6,7,4′-tetrahydroxyflavonol-3-O-rutinoside) were isolated for the first times from the titled subspecies. Conclusions These results confirm that the species of P. atlantica is far from being exhausted of active compounds, especially polyphenols.

scholarly journals Biologically Active Compounds and Antioxidant Activity of the Plants from CSBG SB RAS Collection of the Asteraceae Family

2021 ◽  
Vol 38 ◽  
pp. 00055
Author(s):  
Elena Khramova ◽  
Tatyana Kukushkina ◽  
Tatyana Shaldaeva ◽  
Yuliya Pshenichkina
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Achillea Millefolium ◽  
Artemisia Absinthium ◽  
Tanacetum Vulgare ◽  
Active Compounds ◽  
Artemisia Dracunculus ◽  
Pectin Substances

The article presents Data on the content of biologically active compounds (BAC) and the total phenolic antioxidants activity (TPA) evaluated for leaves and inflorescences of Achillea millefolium, Anthemis tinctoria, Artemisia absinthium, A. dracunculus, Leucanthemum vulgare, Pyrethrum balsamita, P. macrophyllum, Tanacetum vulgare. The leaves and inflorescences of the plants contain flavonols, catechins, tannins, carotenoids, pectin substances. High content of phenolic compounds was found in Artemisia dracunculus and Achillea millefolium (352.34 – 398.99 mg/g of DW). The highest level of catechins was registered in inflorescences (8.01 mg/g) and leaves (4.58 mg/g) of Artemisia absinthium. The content of pectin substances in the studied plants was quite high, with the biggest share of protopectins (50.6 – 95.9 mg/g). The maximum quantity of pectins was found in the leaves and inflorescences of Leucanthemum vulgare (11.2-12.7 mg/g), protopectins – in the leaves of Tanacetum vulgare (95.9 mg/g). The highest content of carotenoids was found in the leaves of plants, except for Leucanthemum vulgare. The highest antioxidant activity was recorded for water-ethanol extracts from leaves of Achillea millefolium (up to 1.09 mg/g) and inflorescences of Artemisia dracunculus (0.94 mg/g) plants due to the increased content of phenolic compounds, in particular flavonols and tannins, compared to the other species.

Phytochemical Analysis of Couropita guianesis Aubl of Leaves

2021 ◽  
pp. 4161-4164
Author(s):  
Shweta R. Patil ◽  
Savita S. Satpute ◽  
Shitalkumar S. Patil
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Anticancer Activity ◽  
Antiulcer Activity ◽  
Phytochemical Analysis ◽  
Pharmacological Effects ◽  
Active Compounds ◽  
Anthelminthic Activity ◽  
Couroupita Guianensis ◽  
Phenolic Substances

Couroupita guianensis Aubl is also called as “Cannon ball tree”. The tree has enormous medicinal values since most of the parts like leaves; flower, fruits and stem are used as medicine to cure various diseases. During the last few decades it has been observed that there are numerous reports on anti-inflammatory activity, antiulcer activity, anticancer activity, antioxidant activity, anthelminthic activity of this plant. It is enriched with a number of compounds such as oils, ketosteroids, glycosides, courouptine, indirubin, isatin and phenolic substances. Phytochemical analysis of the extract showed the presence of major classes of phytochemicals alkaloids, phenolic compounds such as flavonoids, tannins and saponins. Studies reveal that C. guianensis. Aubl. Is a source of medicinally active compounds and have various pharmacological effects.

scholarly journals Kajian Pengaruh Pelarut Terhadap Aktivitas Antioksidan Alga Coklat Genus Sargassum dengan Metode Dpph

2021 ◽  
Vol 3 (2) ◽  
pp. 201-213
Author(s):  
Ni Putu Yunika Candra Riskiana ◽  
Rissa Laila Vifta
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Brown Algae ◽  
Secondary Data ◽  
Journal Review ◽  
Active Compounds ◽  
Effect Of Solvent ◽  
Dpph Method

Brown algae of the genus Sargassum contain phenolic, chlorophyll and carotenoid compounds that function as the most effective antioxidants. The withdrawal of active compounds from natural ingredients is influenced by the nature of the polarity of a solvent. This study aims to examine the effect of solvent variations on antioxidant activity and secondary metabolites in the genus sargassum using the DPPH method.  : This research was conducted using a journal review method using secondary data The solvents used were ethanol and methanol (polar), ethyl acetate (semi polar) and n-hexane (non polar). Antioxidant activity Sargassum in ethyl acetate solvent, namely IC50 = 68.89 mg/L, methanol = 69.27 mg/L, ethanol = 239.51 mg/L and n-hexane = 148 ,16 mg/L. The content of secondary metabolites that have antioxidant activity is phenolic compounds of 1,348.18 mg GAE/g, chlorophyll of 2.84 mg/g and carotenoids of 2.69 mol/g. The solvent variation has an influence on the antioxidant activity. ABSTRAK Alga coklat genus Sargassum mengandung senyawa fenolik, klorofil dan karotenoid yang berfungsi sebagai antioksidan yang paling efektif. Penarikan senyawa aktif dari bahan alam dipengaruhi oleh sifat kepolaran suatu pelarut. Pada penelitian ini bertujuan untuk mengkaji pengaruh variasi pelarut terhadap aktivitas antioksidan dan senyawa metabolit sekunder pada genus (Sargassum) dengan metode DPPH. Penelitian ini dilakukan dengan metode review jurnal menggunakan data sekunder. Pelarut yang digunakan yaitu etanol dan metanol (polar), etil asetat (semi polar) dan n-heksan (non polar). Aktivitas antioksidan (Sargassum) pada pelarut etil asetat yaitu IC50 = 68,89 mg/L, methanol = 69,27 mg/L, etanol = 239,51 mg/L dan n-heksan = 148,16 mg/L. Kandungan senyawa metabolit sekunder yang memiliki aktivitas antioksidan adalah senyawa fenolik sebesar 1.348,18 ± 2.57 mg GAE/g, klorofil  sebesar 2,84 mg/g dan karotenoid sebesar 2,69 µmol/g. Variasi pelarut memberikan pengaruh terhadap aktivitas antioksidan.

scholarly journals Leafy Stems of Phagnalon saxatile subsp. saxatile from Algeriaas a Source of Chlorogenic Acids and Flavonoids with Antioxidant Activity: Characterization and Quantification Using UPLC-DAD-ESI-MSn

Metabolites ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 280
Author(s):  
Farah Haddouchi ◽  
Tarik Mohammed Chaouche ◽  
Riadh Ksouri ◽  
Romain Larbat
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Methanolic Extract ◽  
Total Phenolic ◽  
Chlorogenic Acids ◽  
Synthetic Antioxidants ◽  
Polar Solvents ◽  
Phytochemical Content ◽  
Scavenging Capacity ◽  
Dpph Scavenging

Phagnalon saxatile subsp. saxatile is a wild species widespread in Algeria which is utilized for medicinal purposes as analgesic and anticholesterolemic. However, information is still scarce regarding its phytochemical content. The objective of this study was to identify and quantify the phenolic compounds from different extracts of its leafy stems. For this purpose, the effects of four extracting solvents were investigated on the content of phenolic compounds and the antioxidant activity of this plant. The extracts prepared with polar solvents (methanol and water) contained higher amounts of phenolic compounds and showed better antioxidant activity than the extracts with apolar solvents (hexane, dichloromethane). The methanolic extract, richest in total phenolic and total flavonoid, had significant antioxidant activity as regarded by DPPH° scavenging capacity (IC50 of 5.5 µg/mL), ABTS+° scavenging capacity (IC50 of 63.8 µg/mL) and inhibition of oxidation of linoleic acid (IC50 of 22.7 µg/mL), when compared to synthetic antioxidants. Chlorogenic acids and several flavonoids were identified and quantified by UPLC-DAD-MSn. The di-O-caffeoylquinic acids isomers were the most concentrated phenolics (25.4 mg/g DW) in the methanolic extract.

ORGANIC ACIDS, SUGARS, PHENOLIC COMPOUNDS AND ANTIOXIDANT ACTIVITY OF Malus floribunda coccinella FRUIT, PEEL AND FLESH

2018 ◽  
Vol 17 (5) ◽  
pp. 47-59
Author(s):  
Hacer Coklar ◽  
Mehmet Akbulut ◽  
Iliasu Alhassan ◽  
Şeyma Kirpitci ◽  
Emine Korkmaz
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Organic Acids ◽  
Fruit Peel ◽  
Malus Floribunda

CARBOHYDRATE PRODUCT FOR THERAPEUTIC AND PROPHYLACTIC PURPOSES

2020 ◽  
pp. 89-94
Author(s):  
Khvorova L.S. ◽  
Byzov V.A.
Keyword(s):  
Antioxidant Activity ◽  
Uric Acid ◽  
Phenolic Compounds ◽  
Mental Stress ◽  
The Body ◽  
Hot Water ◽  
Preventive Effect ◽  
Activity Inhibition ◽  
Medicinal Properties ◽  
Simple Carbohydrates

The article is devoted to the creation of a carbohydrate product with rosehip extract in the form of sweets (glucose Fudge) with a therapeutic and preventive effect aimed at restoring the body of people exposed to intense physical and mental stress and the effects of damaging environmental factors. As carbohydrates in the recipe, simple carbohydrates (glucose) and complex carbohydrates in the form of starch molasses containing (maltose, tri - Tetra - sugars, dextrins) are used. Glucose in the formulation is the main energy ingredient, sweetener and structure-forming component that gives the product a solid consistency due to crystallization. The medicinal effect of the product is provided by rosehip extract. Numerous published studies have established its vitamin, immunostimulating and antioxidant activity, inhibition of uric acid formation and obesity, which are associated with flavonoids, other phenolic compounds, and vitamins. The list of medicinal properties of rosehip extract shows the feasibility of using it in additional nutrition of people in order to prevent diseases. For the formulation of our sweets, the extraction of crushed rosehip fruits was carried out three times with hot water at a temperature of 65-700C, followed by concentration of the extract under vacuum to 25-30% SV. The resulting extract-concentrate was introduced into the formulation in a dosage of 5.5% SV per 100 g of SV glucose Fudge, which provided the necessary dosage for the product when used from 3 (for children) to 10 sweets a day (for adults) for the prevention of colds.

Antioxidant Activity, Inhibition α-Glucosidase of Ethanol Extract of Strychnos nitida G. Don and Identification of Active Compounds

2019 ◽  
Vol 5 (3) ◽  
pp. 11-20
Author(s):  
Stefani Dhale Rale ◽  
Hasim Hasim ◽  
Syamsul Falah
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Inhibition Activity ◽  
Active Compounds ◽  
Activity Inhibition ◽  
Activity Test ◽  
Ic50 Value ◽  
Treatment Of Diabetes

This study aims to find the treatment of diabetes using natural materials by exploring plants in the province of East Nusa Tenggara. his research was conducted out by extracting the Strychnos nitida G.Don stem using a method of maceration by ethanol 70%. Ethanol extract was then fractionated using n-hexane and ethyl acetate. Simplicia from maceration and fractionation results were then tested for antioxidant activity, α-glucosidase inhibition activity and identification of active compounds. The results showed that ethyl acetate fraction had the lowest IC50 value of 86.83 μg / ml. Results of the α-glucosidase activity test showed that ethyl acetate fraction and n-heksan fraction at 900 ppm had the highest percentage of inhibition of 34.23% and 33.89%. Identification using LCMS/MS method showed that ethyl acetate fraction consist of Benzenemethamine, N, N-dioctyl- as an antioxidantcompound and compound 24-methyl-5-cholestone-hexol as an antidiabetic compound. From the results of this study, we concluded that the extract of kayu ular Strychnos nitida G.Don stem has inhibition activity toward α-glucosidase enzyme.

Extraction of Phenolic Compounds with Antioxidant Activity from Beetroot (Beta vulgaris L.)

2018 ◽  
Vol 14 (4) ◽  
pp. 350-357 ◽  
Author(s):  
Raquel P.F. Guine ◽  
Fernando Goncalves ◽  
Clemence Lerat ◽  
Theo El Idrissi ◽  
Eva Rodrigo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Beta Vulgaris
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