Ion regulation in the antennal glands differs among Ocypodoidea and Grapsoidea crab species

2020 ◽  
Vol 248 ◽  
pp. 110753
Author(s):  
Kuang-Yu Tseng ◽  
Jyuan-Ru Tsai ◽  
Hui-Chen Lin
Keyword(s):  
Crab Species ◽  
Ion Regulation ◽  
Antennal Glands

Microanatomy of the antennal glands and their Na+/K+-ATPase activity in three true crab species (Brachyura), Portunus pelagicus (Linnaeus, 1758) (Portunidae), Macrophthalmus dentipes Lucas in Guérin, 1836 (Macrophthalmidae) and Eriocheir hepuensis Dai, 1991 (Varunidae)

Crustaceana ◽  
2021 ◽  
Vol 94 (11-12) ◽  
pp. 1359-1376
Author(s):  
Khadijeh Khalifi ◽  
Negin Salamat ◽  
Abdolali Movahedinia
Keyword(s):  
Posterior Part ◽  
Tissue Structure ◽  
Freshwater Species ◽  
Crab Species ◽  
Anterior Portion ◽  
Portunus Pelagicus ◽  
Posterior Portion ◽  
Seawater Adaptation ◽  
Pump Activity ◽  
Antennal Glands

Abstract There are differences between various crab species in the function and structure of organs involved in ionic and osmotic regulation processes. The antennal glands together constitute one of the most important organs involved in the osmoregulation in crabs. The present investigation aimed to study the tissue structure of the antennal glands and their Na+/K+-ATPase (NKA) pump activity in three true crab species from three different habitats, including the marine (Portunus pelagicus (Linnaeus, 1758)), the estuarine (Macrophthalmus dentipes Lucas in Guérin, 1836) and the freshwater habitat (Eriocheir hepuensis Dai, 1991). In this regard, the tissue structure of the antennal glands and the activity of the Na+/K+-ATPase (NKA) pump were assessed in these three selected species. The results showed that the antennal glands in all studied species consisted of two anterior parts and a posterior part. The anterior parts are composed of the proximal tubular region (PT) and the distal tubular region (DT). The PT and DT parts comprised the coelomosac and labyrinths in the anterior portion, and the bladder located in the posterior portion. However, despite the similarity in the general tissue structure of the antennal gland in marine, estuarine and freshwater crab species, some structural differences were observed between those species. Labyrinth cells, coelomosac podocytes, and bladder cells in the estuarine crab M. dentipes contained large vacuoles especially on the top (i.e., near the lumen) of the cells. The highest amount of NKA pump activity was measured in the antennal glands of M. dentipes (). The NKA pump plays a more important role in the estuary and seawater adaptation of crabs, but freshwater species are not highly dependent on the NKA enzyme for osmoregulation.

Metabolism, homeostasis and growth

2017 ◽  
Author(s):  
Derek Burton ◽  
Margaret Burton
Keyword(s):  
Energy Storage ◽  
White Muscle ◽  
External Environment ◽  
Life Cycles ◽  
Fish Growth ◽  
Chloride Cells ◽  
Internal Environment ◽  
Ion Regulation ◽  
Yolk Proteins ◽  
Active Substances

Metabolism consists of the sum of anabolism (construction) and catabolism (destruction) with the release of energy, and achieving a fairly constant internal environment (homeostasis). The aquatic external environment favours differences from mammalian pathways of excretion and requires osmoregulatory adjustments for fresh water and seawater though some taxa, notably marine elasmobranchs, avoid osmoregulatory problems by retaining osmotically active substances such as urea, and molecules protecting tissues from urea damage. Ion regulation may occur through chloride cells of the gills. Most fish are not temperature regulators but a few are regional heterotherms, conserving heat internally. The liver has many roles in metabolism, including in some fish the synthesis of antifreeze seasonally. Maturing females synthesize yolk proteins in the liver. Energy storage may include the liver and, surprisingly, white muscle. Fish growth can be indeterminate and highly variable, with very short (annual) life cycles or extremely long cycles with late and/or intermittent reproduction.

Toxicity of petroleum hydrocarbons to Brachyuran crabs: a review of deleterious effects of oil-related xenobiotics on life stages

2021 ◽  
pp. 1-16
Author(s):  
Qusaie Karam ◽  
Zainab Al-Wazzan
Keyword(s):  
Petroleum Hydrocarbons ◽  
Marine Pollution ◽  
Human Consumption ◽  
Life Stages ◽  
Crab Species ◽  
Research Attention ◽  
Larval Stages ◽  
Effects Of Ph ◽  
Ph Toxicity ◽  
Brachyuran Crabs

Abstract Petroleum hydrocarbons (PH) toxicity and bioaccumulation in aquatic organisms have been investigated for almost 50 years. Continuous oil spillages necessitate a further understanding of the toxicological effects of PH on brachyuran crabs. Crabs can be exposed to PH through various routes such as the water column, sediment and diet. Numerous investigations have been dedicated to evaluating PH toxicity on different life stages of crab species, but the majority of them have focused on the blue crab Callinectes sapidus as it represents an edible and favourable seafood commodity for human consumption. The objective of the review is to critically assess studies related to PH toxicity on different life stages of 41 crab species representing 13 families across the world. Several physiological, biochemical and genetic endpoints of marine crabs were evaluated in addition to the sublethal effects of PH on crab metabolism, behaviour, moulting, growth and survival. A concise summary of PH deleterious effects on different taxonomic species of marine crabs is discussed and provides evidence that crabs can be used as indicator organisms of biomarker significance for marine pollution. Overall, larval stages appeared to be the most sensitive to the deleterious effects of PH compared with juveniles and adults. However, adult stages have received more research attention than other life stages, followed by larval stages, and juvenile stages are the least investigated stages with respect to PH toxicity. Finally, hepatopancreas and gills were the organs where greatest accumulation of PH was recorded.

Osmoregulation in the Lobster Homarus americanus

1970 ◽  
Vol 27 (6) ◽  
pp. 1123-1130 ◽  
Author(s):  
W. Dall
Keyword(s):  
Freezing Point ◽  
Homarus Americanus ◽  
Salinity Range ◽  
Limited Extent ◽  
Salt Loading ◽  
Excess Water ◽  
Salt Excretion ◽  
Rapid Adjustment ◽  
Antennal Glands

Lobsters took 72 hr to adapt to a salinity of 20‰, with urine becoming markedly hypoosmotic to the blood during the first 25 hr, then increasing in concentration over the next 48 hr, though remaining significantly hypoosmotic to the blood. Adaptation to a salinity of 37‰ took 24 hr: the urine became almost isosmotic with the blood: the gastric and rectal fluids became hyperosmotic to the blood. Blood freezing-point determinations showed that over the salinity range 20–37‰ the lobster is able to osmoregulate to a limited extent only towards the lower end of the range, being otherwise an "osmocon-former." Salt-loading experiments indicated that the excess salts were rapidly excreted into the gut. It is concluded that the antennal glands are at least partly responsible for elimination of excess water, but that the gut is the site of salt excretion, and that there is rapid adjustment of salt imbalance.

162 EFFECTS OF LACTACIDOSIS ON ION REGULATION IN RESTING CAT HINDLIMB

1993 ◽  
Vol 25 (Supplement) ◽  
pp. S30
Author(s):  
M. I. Lindinger ◽  
P. D. Watson ◽  
M. T. Hamilton ◽  
D. S. Ward
Keyword(s):  
Ion Regulation

Estrogen-related receptor γ2 controls NaCl uptake to maintain ionic homeostasis

2021 ◽  
Author(s):  
Shang-Wu Shih ◽  
Jia-Jiun Yan ◽  
Yi-Hsing Wang ◽  
Yi-Ling Tsou ◽  
Ling Chiu ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Body Fluid ◽  
Whole Body ◽  
Ionic Homeostasis ◽  
Ion Regulation ◽  
Expression Levels ◽  
Morpholino Knockdown ◽  
Oryzias Melastigma ◽  
Euryhaline Teleost

Estrogen-related receptors (ERRs) are known to function in mammalian kidney as key regulators of ion transport-related genes; however, a comprehensive understanding of the physiological functions of ERRs in vertebrate body fluid ionic homeostasis is still elusive. Here, we used medaka (Oryzias melastigma), a euryhaline teleost, to investigate how ERRs are involved in ion regulation. After transferring medaka from hypertonic seawater to hypotonic freshwater (FW), the mRNA expression levels of errγ2 were highly upregulated, suggesting that ERRγ2 may play a crucial role in ion uptake. In situ hybridization and immunofluorescence staining showed that errγ2 was specifically expressed in ionocytes, the cells responsible for Na+/Cl- transport. In normal FW, ERRγ2 morpholino knockdown caused reductions in the mRNA expression of Na+/Cl- cotransporter (NCC), the number of NCC ionocytes, Na+/Cl- influxes of ionocytes, and whole-body Na+/Cl- contents. In FW with low Na+ and low Cl-, the expression levels of mRNA for Na+/H+ exchanger 3 (NHE3) and NCC were both decreased in ERRγ2 morphants. Treating embryos with DY131, an agonist of ERRγ, increased the whole-body Na+/Cl- contents and ncc mRNA expression in ERRγ2 morphants. As such, medaka ERRγ2 may control Na+/Cl- uptake by regulating ncc and/or nhe3 mRNA expression and ionocyte number, and these regulatory actions may be subtly adjusted depending on internal and external ion concentrations. These findings not only provide new insights into the underpinning mechanism of actions of ERRs, but also enhance our understanding of their roles in body fluid ionic homeostasis for adaptation to changing environments during vertebrate evolution.

scholarly journals Thermal tolerance of the zoea I stage of four Neotropical crab species (Crustacea: Decapoda)

2018 ◽  
Vol 35 ◽  
pp. 1-5
Author(s):  
Adriana P. Rebolledo ◽  
Rachel Collin
Keyword(s):  
Thermal Tolerance ◽  
Elevated Temperatures ◽  
Marine Invertebrates ◽  
Negative Impact ◽  
Larval Survival ◽  
Crab Species ◽  
Marine Habitat ◽  
Mangrove Species ◽  
Larval Stages ◽  
Carry Over

. Although larval stages are often considered particularly vulnerable to stressors, for many marine invertebrates studies of thermal tolerance have focused on adults. Here we determined the upper thermal limit (LT50) of the zoea I of four Caribbean crab species (Macrocoelomatrispinosum, Aratuspisonii, Armasesricordi, and Minucarapax) and compared their thermal tolerance over time and among species. The zoea from the subtidal species M.trispinosum and tree climbing mangrove species A.pisonii had a lower thermal tolerance, 35 and 38.5 °C respectively, than did the semiterrestrial A.ricordi and M.rapax. In all four species tested, the estimates of thermal tolerance depend on the duration of exposure to elevated temperatures. Longer exposures to thermal stress produce lower estimates of LT50, which decreased by ~1 °C from a two- to a six-hour exposure. Crab embryos develop on the abdomen of the mother until the larvae are ready to hatch. Therefore, the thermal tolerances of the embryos which need to coincide with the environmental conditions experienced by the adult stage, may carry over into the early zoea stage. Our results suggest that semiterrestrial species, in which embryos may need to withstand higher temperatures than embryos of subtidal species also produce larvae with higher thermal tolerances. Over the short term, the larvae of these tropical crab species can withstand significantly higher temperatures than those experienced in their marine habitat. Longer term rearing studies are necessary to determine the temperature at which chronic exposure has a negative impact on embryonic and larval survival.

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