Ovicidal, larvicidal and pupicidal efficacy of silver nanoparticles synthesized by Bacillus marisflavi against the chosen mosquito species

Microbial synthesis of silver nanoparticles is more advantageous and is eco-friendly to combat the various vectors that cause diseases in humans. Hence, in the present study a Bacillus strain is isolated from marine habitat and is evaluated for its ability to synthesize silver nanoparticles (AgNPs) and its efficacy evaluated against the immature stages of selected mosquito species. The effective candidate was confirmed to be Bacillus marisflavi after 16S rRNA sequencing. The synthesis of AgNPs was confirmed by UV-Vis spectrophotometer. Atomic Force Microscopic (AFM) analysis showed spherical nanoparticles. Size analysis using Scanning Electron Microscope (SEM) showed particles of nano size averaging 78.77 nm. The diameter of the particles analyzed by Dynamic Light Scattering (DLS) showed 101.6 nm with a poly-dispersive index of 0.3. Finally the elemental nature of the nanoparticles was identified by Fourier-transform infrared spectroscopy (FTIR). LC50 and LC90 values for the ovicidal, larvicidal and pupicidal efficacy of the AgNPs against the egg, larvae and pupae of Aedes aegypti, Culex quinquefasciatus and Anopheles stephensi respectively were evaluated. The present study revealed that the nanoparticles have an excellent toxic effect against the disease transmitting vector mosquitoes. Hence, the rapid synthesis of AgNPs would be an appropriate eco-friendly tool for biocontrol of vector mosquitoes.

Status, Threats and Conservation of the Wetland Ecosystem Dominated with Mangroves in India

Mangroves are one of the most undermined biological systems. They have immense ecological and economic advantages. Consistent, increment in natural and coastal development like a change of wetlands for hydroponics, construction for tourism, the tsunami has led to the demolishment of mangrove environments in India. The inhabitants of minor zones are at risk of losing their livelihood and there is a threat to the existence of the mangrove ecosystem. It has been noted that from a decade ago, about 40% of the Indian mangrove territories have been lost. At present, the environmental changes and increased anthropogenic activities have become a significant concern for the conservation and sustaining of the mangroves. Conservation of mangrove environment can be accomplished by increasing awareness about their significance among various stakeholders and safeguarding them through mandatory national policy and regulations. A specific policy like Coastal Zone Regulation has been formulated to ensure mangrove conservation, sustainable existence of local community habitat along the coastal area, fishing community, and coastal stretches for marine habitat have been protected.

Sesquiterpenoids Specially Produced by Fungi: Structures, Biological Activities, Chemical and Biosynthesis (2015–2020)

Fungi are widely distributed in the terrestrial environment, freshwater, and marine habitat. Only approximately 100,000 of these have been classified although there are about 5.1 million characteristic fungi all over the world. These eukaryotic microbes produce specialized metabolites and participate in a variety of ecological functions, such as quorum detection, chemical defense, allelopathy, and maintenance of symbiosis. Fungi therefore remain an important resource for the screening and discovery of biologically active natural products. Sesquiterpenoids are arguably the richest natural products from plants and micro-organisms. The rearrangement of the 15 high-ductility carbons gave rise to a large number of different skeletons. At the same time, abundant structural variations lead to a diversification of biological activity. This review examines the isolation, structural determination, bioactivities, and synthesis of sesquiterpenoids that were specially produced by fungi over the past five years (2015‒2020).

Enabling and Shaping Decommissioning Alternatives in Malaysia

This paper is to demonstrate the significance of structured planning, holistic assessment and synergies, as key value drivers in enabling and shaping decommissioning alternatives leading to sustainable decommissioning and circular economy. PETRONAS as the regulator of Malaysia's Upstream activities manage decommissioning obligations through Production Sharing Contracts, internal guidelines and other relevant procedures and standards. The Decommissioning Options Assessment (DOA) is the process used to land on the most feasible option. Throughout PETRONAS’ 18-year decommissioning journey thus far, decommissioning projects were successfully executed using various alternatives. The valuable learnings gained are applied to further strengthen our decommissioning processes in regulating, enabling and shaping future executions at the lowest cost with safety of life and protection of the environment as our utmost priority. Upon a decision to proceed with decommissioning, a gated technical review process is used as the governing process to ensure safety, protection to the environment and cost efficiency. It is during this gated technical review that DOA is conducted. The output from the DOA is deliberated within the ambit of five (5) key criteria, i.e. Health, Safety & Security, Environment, Society, Technical & Operational, and Economy. Upon completion of execution, lessons learnt coupled with findings from post-decommissioning surveys are analyzed and applied to future projects. Synergies and collaborations are key drivers in shaping sustainable and replicable alternative decommissioning solutions. PETRONAS continuously pursues strategic collaborations with all stakeholders, including but not limited to, government ministries/agencies, academia, and industry players to tap into global decommissioning solutions, scientific researches, technologies, and best practices. This key lever will be discussed in the paper. From actual experiences, supported by studies, it is evident that decommissioning alternatives, including Rigs-to-Reef, add value in terms of marine habitat protection, biodiversity enhancement, fish aggregation, etc. It has also contributed positively to the livelihoods and well-being of society. Re-using platforms for new field development maximizes value by extending the platform's useful life. In addition, PETRONAS also advocates the ‘design for decommissioning’ mindset during a field's development phase in achieving a robust life cycle cost. PETRONAS further believes the values gained from these decommissioning alternatives will contribute to the decommissioning ecosystem in Malaysia. Moving forward, PETRONAS aspires to elevate the sustainable decommissioning model with the mindset that, ‘no single piece of an abandoned structure goes to waste’. There is a need to mature studies, collaborations and supply chain readiness in realizing more options on the 3Rs (Reduce, Re-use and Recycle).

Taxonomically and Functionally Distinct Ciliophora Assemblages Inhabiting Baltic Sea Ice

Ciliophora is a phylum of unicellular eukaryotes that are common and have pivotal roles in aquatic environments. Sea ice is a marine habitat, which is composed of a matrix of solid ice and pockets of saline water in which Ciliophora thrive. Here, we used phylogenetic placement to identify Ciliophora 18S ribosomal RNA reads obtained from wintertime water and sea ice, and assigned functions to the reads based on this taxonomic information. Based on our results, sea-ice Ciliophora assemblages are poorer in taxonomic and functional richness than under-ice water and water-column assemblages. Ciliophora diversity stayed stable throughout the ice-covered season both in sea ice and in water, although the assemblages changed during the course of our sampling. Under-ice water and the water column were distinctly predominated by planktonic orders Choreotrichida and Oligotrichida, which led to significantly lower taxonomic and functional evenness in water than in sea ice. In addition to planktonic Ciliophora, assemblages in sea ice included a set of moderately abundant surface-oriented species. Omnivory (feeding on bacteria and unicellular eukaryotes) was the most common feeding type but was not as predominant in sea ice as in water. Sea ice included cytotrophic (feeding on unicellular eukaryotes), bacterivorous and parasitic Ciliophora in addition to the predominant omnivorous Ciliophora. Potentially mixotrophic Ciliophora predominated the water column and heterotrophic Ciliophora sea ice. Our results highlight sea ice as an environment that creates a set of variable habitats, which may be threatened by the diminishing extent of sea ice due to changing climate.

Strontium isotopes reveal diverse life history variations, migration patterns, and habitat use for Broad Whitefish (Coregonus nasus) in Arctic, Alaska

Conservation of Arctic fish species is challenging partly due to our limited ability to track fish through time and space, which constrains our understanding of life history diversity and lifelong habitat use. Broad Whitefish ( Coregonus nasus ) is an important subsistence species for Alaska’s Arctic Indigenous communities, yet little is known about life history diversity, migration patterns, and freshwater habitat use. Using laser ablation Sr isotope otolith microchemistry, we analyzed Colville River Broad Whitefish 87 Sr/ 86 Sr chronologies (n = 61) to reconstruct movements and habitat use across the lives of individual fish. We found evidence of at least six life history types, including three anadromous types, one semi-anadromous type, and two nonanadromous types. Anadromous life history types comprised a large proportion of individuals sampled (collectively, 59%) and most of these (59%) migrated to sea between ages 0–2 and spent varying durations at sea. The semi-anadromous life history type comprised 28% of samples and entered marine habitat as larvae. Nonanadromous life history types comprised the remainder (collectively, 13%). Otolith 87 Sr/ 86 Sr data from juvenile and adult freshwater stages suggest that habitat use changed in association with age, seasons, and life history strategies. This information on Broad Whitefish life histories and habitat use across time and space will help managers and conservation planners better understand the risks of anthropogenic impacts and help conserve this vital subsistence resource.

Preface: Proceedings of the 4th International Conference on Bioscience and Biotechnology (4th ICBB 2021), 21st-23rd September 2021.

Eka Sunarwidhi Prasedya, S.Si., M.Sc., Ph.D. Chairman of 4th ICBB 2021 This issue of IOP conference proceedings contains papers presented at the 4th International Conference on Bioscience and Biotechnology (ICBB) 2021. The ICBB is an annual international conference since 2018 that was initiated by Prof Ir H Sunarpi PhD. This year we are lucky to collaborate with Postgraduate-studies (Pascasarjana) University of Mataram thanks to the director Prof. Ir. H Muhamad Sarjan, M.Agr.,C.P. Ph.D and vice director Prof. Ir. Bambang Hari Kusumo, M.Agr.St., Ph.D. This meeting is hosted by Lab of Bioscience and Biotechnology Research, University of Mataram, Indonesia. It is held for three days, from 21 to 23 September 2021 by virtual conference with zoom platform due to COVID-19 pandemic. Interactive online presentations were arranged through Zoom Video Communications for the participants to present their ideas. The conference was divided into two main sessions: Main session for Keynote speakers and Panel Sessions for participants. A time of 30 minutes was given for Keynote speakers to present their fabulous work. For panel session, 10 minutes were given for participants to share their research and findings. A total of 204 participants (180 presenters and 24 non-presenters) from Universities, Research Institutes and also Government Departments joined the conference. All the selected papers were peer reviewed by expert reviewers in a double blind review system as per the review policy given by IOP Conference Series. On the first day of the conference (21st September) invited talks were presented by Prof. Julian Heyes, Ph.D. from Massey University New Zealand on “Deriving value from elite indigenous fruit and vegetable species”, Prof. Bambang Hari Kusumo, M.Agr.St., Ph.D. from University of Mataram on “Rapid measurement of soil carbon using near infrared technology”and Prof. Dr. Endang Semiarti, M.S., M.Sc. from Universitas Gadjah Mada Indonesia on “Biotechnology approach to improve the quality and quantity of orchids as potential agricultural commodities in Indonesia”. The second day of the conference presented talks by Prof. Lim Phaik Eem, Ph.D. from University of Malaya on “Importance of marine habitat conservation for utilization and discovery of new bioresources of seaweeds”, Prof. Mat Vanderklift, Ph.D. from Indian Ocean Marine Research Centre CSIRO Australia on “Opportunities for sustainable use of coastal ecosystems”, Assoc.Prof. Dr. Rapeeporn Ruangchuay from Prince of Songkla University Thailand on “ Seaweed resources in Thailand: cultivation and utilization” and Dr.rer.nat. Andri Frediansyah, M.Sc. from LIPI Indonesia on ”Microbial natural products: a discovery strategy”. The final day of the conference included talks by Prof. Akihiro Hazama, Ph.D. from Fukushima Medical University on “Investigation of cigarette smoke-induced cell hyperplasia mechanism using Induced Pluripotent Stem Cell (IPSCs)”, Prof. Kato Yasuhiro, Ph.D. from Keio University Japan on ”Water circulation of the earth and life” and Eka Sunarwidhi Prasedya, Ph.D. from University of Mataram Indonesia on “Microbiome implications for bioprospecting of seaweeds”. List of Conference Photograph, Conference Organizers are available in this pdf.

Marbled Murrelets prefer stratified waters close to freshwater inputs in Haida Gwaii, British Columbia, Canada

The Marbled Murrelet (Brachyramphus marmoratus) is a small seabird that is currently listed as threatened in Canada. Understanding this species’ marine habitat preferences plays a vital role in our ability to focus conservation planning. We used the longest-running at-sea survey dataset available in British Columbia to examine hotspot persistence and habitat use at Laskeek Bay, Haida Gwaii, BC. The Laskeek Bay Conservation Society has been conducting spring and summer surveys along fixed transect routes in open and shoreline waters from 1997 to 2018. Along with analyzing this long-term dataset, we conducted surveys to measure oceanographic variables (2018–2019) and tested whether Marbled Murrelets in the same area used prey and oceanographic information to select marine habitat in conjunction with physical habitat features. Our hotspot persistence map, defined as areas that repeatedly had counts above a 75% threshold relative to other areas during a given survey, showed that murrelets consistently preferred shoreline transects. Murrelets also preferred shallow marine areas closer to streams, above higher proportions of sandy substrate and closer proximity to abundant nesting habitat. Modeling weather and time variables contributed little additional predictive power. Nonetheless, models that included physical environmental, oceanographic, and prey variables outperformed those with only physical environmental variables. Stratified water was the oceanographic variable most strongly related to higher counts. Our study suggests that stratified waters could work with stream systems to create productive zones for foraging murrelets, and highlights the importance of murrelets having access to marine areas with the preferred physical features.