Identification, expression analysis, and antibacterial activity of Apolipoprotein A-I from amphioxus (Branchiostoma belcheri)

2019 ◽  
Vol 238 ◽  
pp. 110329 ◽  
Author(s):  
Wei Wang ◽  
Qing Qu ◽  
Jianming Chen
Keyword(s):  
Antibacterial Activity ◽  
Expression Analysis ◽  
Apolipoprotein A ◽  
Branchiostoma Belcheri

Abstract 545: Differential mRNA Expression is Influenced by Apolipoprotein A-I in Order to Promote Foam Cell Regression

2016 ◽  
Vol 36 (suppl_1) ◽  
Author(s):  
Elisa C Maruko ◽  
Hao Xu ◽  
Sushma Kaul ◽  
Brian J Capaldo ◽  
Nathalie Pamir ◽  
...  
Keyword(s):  
Gene Expression ◽  
Differential Expression ◽  
Expression Analysis ◽  
Immune Cell ◽  
Foam Cell ◽  
Differential Expression Analysis ◽  
Ldl Receptor ◽  
Gene Set Enrichment Analysis ◽  
Control Group ◽  
Apolipoprotein A

Atherosclerosis is a disease of both lipids and inflammatory immune cells. More specifically, elevated plasma levels of low-density lipoproteins (LDL) leads to migration of circulating monocytes into the artery wall. Lipid loaded monocyte cells subsequently proliferate in the arterial walls becoming macrophage foam cells; a hallmark of atherosclerotic lesions. A proposed mechanism of the protective effects of high-density lipoprotein (HDL) is apolipoprotein A-I (apo A-I) acting as a mediator of cholesterol efflux and subsequent foam cell regression. To better understand the biological changes stimulated by apo A-I treatment, differential expression analysis of microarray data was performed on spleen cells from apo A-I treated mice. LDL receptor null (LDLr -/- ) and LDL receptor and apo A-I null (LDLr -/- , apoA-I -/- ) mice were fed a western diet consisting of 0.2% cholesterol and 42% of calories as fat for 12 weeks. After 6 weeks of diet, a subset of mice for each genotype was subcutaneously injected with 200 micrograms of apo A-I 3 times a week for the remaining 6 weeks. The control group mice were subcutaneously injected with 200 micrograms of saline or BSA. Spleen cell RNA was isolated, purified, and analyzed for differential expression analysis using Illumina BeadArray Microarray Technology Analysis. Individual gene expression analysis for LDLr -/- , apoA-I -/- apo A-I treated mice showed 281 significantly differentially expressed genes compared to BSA treated mice. LDLr -/- A-I treated mice had 1502. Of the significant genes, 189 intersected across both genotypes. LDLr -/- , apoA-I -/- A-I mice showed 73 up-regulated and 116 down-regulated genes. Similarly, LDLr -/- A-I mice had 71 up-regulated and 118 down-regulated. One-directional Gene Set Enrichment Analysis (GSEA) of LDLr -/- , apoA-I -/- A-I mice revealed 49 significant pathways while a total of 63 were found for LDLr -/- . Of these pathways, 21 were up-regulated and 13 were down-regulated in both genotypes. Eight of the top 10 most significant up-regulated pathways in both genotypes were immune cell related. Their functions involve receptor, adhesion, and chemokine signaling. Overall, preliminary analysis suggests A-I treatment induces similar gene expression changes across different genotypes.

Apolipoprotein A-I from striped bass (Morone saxatilis) demonstrates antibacterial activity in vitro

2008 ◽  
Vol 151 (2) ◽  
pp. 167-175 ◽  
Author(s):  
L. Danielle Johnston ◽  
Gwynne Brown ◽  
David Gauthier ◽  
Kimberly Reece ◽  
Howard Kator ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Striped Bass ◽  
Morone Saxatilis ◽  
Apolipoprotein A

scholarly journals Genome-wide gene expression analysis in the amphioxus, Branchiostoma belcheri after poly (I: C) challenge using strand-specific RNA-seq

Oncotarget ◽  
2017 ◽  
Vol 8 (65) ◽  
pp. 108392-108405 ◽  
Author(s):  
Qi-Lin Zhang ◽  
Zheng-Qing Xie ◽  
Ming-Zhong Liang ◽  
Bang Luo ◽  
Xiu-Qiang Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Rna Seq ◽  
Genome Wide ◽  
Branchiostoma Belcheri ◽  
Genome Wide Gene Expression

Identification, expression analysis, and antibacterial activity of NK-lysin from common carp Cyprinus carpio

2018 ◽  
Vol 73 ◽  
pp. 11-21 ◽  
Author(s):  
Gai Ling Wang ◽  
Ming Cheng Wang ◽  
Ying Li Liu ◽  
Qian Zhang ◽  
Chuan Feng Li ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Common Carp ◽  
Expression Analysis ◽  
Cyprinus Carpio ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio

Ultrastructural localization and antibacterial activity of phenoloxidase in amphioxus Branchiostoma belcheri tsingtauense

2001 ◽  
Vol 81 (4) ◽  
pp. 705-706 ◽  
Author(s):  
Guorong Li ◽  
Shicui Zhang ◽  
Hongyan Li
Keyword(s):  
Antibacterial Activity ◽  
Epithelial Cells ◽  
Active Material ◽  
Specific Inhibitor ◽  
Ultrastructural Localization ◽  
The Body ◽  
Reaction Products ◽  
E Coli ◽  
Branchiostoma Belcheri ◽  
Secondary Lysosomes

Phenoloxidase (PO) has been shown to be present in both invertebrates and vertebrates, yet little is known about the PO in Branchiostoma belcheri tsingtauense. The present study demonstrated that the PO activity existed histochemically the epithelial cells of the gill bar and intestine of adult animal, producing brown to black melanin deposits. Ultrastructural examination revealed that PO reaction products were localized in the cytoplasmic granules of the epidermal cells, the epithelial cells of gill bar and intestine and most of them were electron-dense and homogenous. Frequently, the PO reaction products were also observed in the secondary lysosomes.  The mucus from the body surface had little antibacterial activity against Escherichia coli, but it enhanced the antibacterial activity of L-dopa and the enhancement was suppressed by PTU, a PO specific inhibitor. This indicates that the active material inhibiting the E. coli growth in the mucus is PO.

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