Synthesis of CeO2 as promising adsorbent for the management of free-DNA harboring antibiotic resistance genes from tap-water

2020 ◽  
Vol 401 ◽  
pp. 125562 ◽  
Author(s):  
Eric Tobechukwu Anthony ◽  
Mike O. Ojemaye ◽  
Anthony I. Okoh ◽  
Omobola O. Okoh
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Tap Water ◽  
Antibiotic Resistance Genes ◽  
Free Dna

scholarly journals Environmental Spread of New Delhi Metallo-β-Lactamase-1-Producing Multidrug-Resistant Bacteria in Dhaka, Bangladesh

2017 ◽  
Vol 83 (15) ◽  
Author(s):  
Mohammad Aminul Islam ◽  
Moydul Islam ◽  
Rashedul Hasan ◽  
M. Iqbal Hossain ◽  
Ashikun Nabi ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Tap Water ◽  
Antibiotic Resistance Genes ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
New Delhi ◽  
Multidrug Resistant Bacteria ◽  
Content Type ◽  
Wastewater Samples

ABSTRACT Resistance to carbapenem antibiotics through the production of New Delhi metallo-β-lactamase-1 (NDM-1) constitutes an emerging challenge in the treatment of bacterial infections. To monitor the possible source of the spread of these organisms in Dhaka, Bangladesh, we conducted a comparative analysis of wastewater samples from hospital-adjacent areas (HAR) and from community areas (COM), as well as public tap water samples, for the occurrence and characteristics of NDM-1-producing bacteria. Of 72 HAR samples tested, 51 (71%) samples were positive for NDM-1-producing bacteria, as evidenced by phenotypic tests and the presence of the bla NDM-1 gene, compared to 5 of 41 (12.1%) samples from COM samples (P < 0.001). All tap water samples were negative for NDM-1-producing bacteria. Klebsiella pneumoniae (44%) was the predominant bacterial species among bla NDM-1-positive isolates, followed by Escherichia coli (29%), Acinetobacter spp. (15%), and Enterobacter spp. (9%). These bacteria were also positive for one or more other antibiotic resistance genes, including bla CTX-M-1 (80%), bla CTX-M-15 (63%), bla TEM (76%), bla SHV (33%), bla CMY-2 (16%), bla OXA-48-like (2%), bla OXA-1 (53%), and bla OXA-47-like (60%) genes. Around 40% of the isolates contained a qnr gene, while 50% had 16S rRNA methylase genes. The majority of isolates hosted multiple plasmids, and plasmids of 30 to 50 MDa carrying bla NDM-1 were self-transmissible. Our results highlight a number of issues related to the characteristics and source of spread of multidrug-resistant bacteria as a potential public health threat. In view of the existing practice of discharging untreated liquid waste into the environment, hospitals in Dhaka city contribute to the potential dissemination of NDM-1-producing bacteria into the community. IMPORTANCE Infections caused by carbapenemase-producing Enterobacteriaceae are extremely difficult to manage due to their marked resistance to a wide range of antibiotics. NDM-1 is the most recently described carbapenemase, and the bla NDM-1 gene, which encodes NDM-1, is located on self-transmissible plasmids that also carry a considerable number of other antibiotic resistance genes. The present study shows a high prevalence of NDM-1-producing organisms in the wastewater samples from hospital-adjacent areas as a potential source for the spread of these organisms to community areas in Dhaka, Bangladesh. The study also examines the characteristics of the isolates and their potential to horizontally transmit the resistance determinants. The significance of our research is in identifying the mode of spread of multiple-antibiotic-resistant organisms, which will allow the development of containment measures, leading to broader impacts in reducing their spread to the community.

scholarly journals The occurrence of antibiotic resistance genes in tap water – a review

2018 ◽  
Vol 30 ◽  
pp. 01012 ◽  
Author(s):  
Agata Siedlecka
Keyword(s):  
Drinking Water ◽  
Antibiotic Resistance ◽  
Dna Extraction ◽  
Resistance Genes ◽  
Tap Water ◽  
Antibiotic Resistance Genes ◽  
Isolation Procedure ◽  
Genetic Determinants ◽  
Treated Drinking Water ◽  
Final Amount

The study presents a review of the occurrence of genetic determinants of antibiotic resistance in tap water. The aim of this study was also to compare the applied methods for antibiotic resistance genes (ARGs) investigations in tap water. As the concentration of ARGs in treated, drinking water is expected to be very low and may cause problems in a standard isolation procedure, the special emphasis is placed on the applied procedures of DNA extraction and their efficiency. The study presents the first attempts to obtain DNA directly from tap water. Further efforts must be put to determine the final amount of obtained DNA and the presence of chosen ARGs among the molecules.

Cell-free DNA: A Neglected Source for Antibiotic Resistance Genes Spreading from WWTPs

2017 ◽  
Vol 52 (1) ◽  
pp. 248-257 ◽  
Author(s):  
Yan Zhang ◽  
Aolin Li ◽  
Tianjiao Dai ◽  
Feifei Li ◽  
Hui Xie ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Cell Free Dna ◽  
Free Dna

scholarly journals Tracing Antibiotic Resistance Genes along the Irrigation Water Chain to Chive: Does Tap or Surface Water Make a Difference?

Antibiotics ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1100
Author(s):  
Maria-Theresia Gekenidis ◽  
Fiona Walsh ◽  
David Drissner
Keyword(s):  
Water Quality ◽  
Antibiotic Resistance ◽  
Surface Water ◽  
Resistance Genes ◽  
Irrigation Water ◽  
Tap Water ◽  
High Capacity ◽  
Antibiotic Resistance Genes ◽  
Resistance Determinants ◽  
Potential Source

Irrigation water is well known as potential source of pathogens in fresh produce. However, its role in transferring antibiotic resistance determinants is less well investigated. Therefore, we analyzed the contribution of surface and tap water to the resistome of overhead-irrigated chive plants. Field-grown chive was irrigated with either surface water (R-system) or tap water (D-system), from planting to harvest. Water along the two irrigation chains as well as the respective plants were repeatedly sampled and screened for 264 antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs), using high-capacity qPCR. Differentially abundant (DA) ARGs were determined by comparing the two systems. On R-chive, β-lactam ARGs, multidrug-resistance (MDR) determinants, and MGEs were most abundant, while D-chive featured DA ARGs from the vancomycin class. Diversity and number of DA ARGs was the highest on young chives, strongly diminished at harvest, and increased again at the end of shelf life. Most ARGs highly enriched on R- compared to D-chive were also enriched in R- compared to D-sprinkler water, indicating that water played a major role in ARG enrichment. Of note, blaKPC was detected at high levels in surface water and chive. We conclude that water quality significantly affects the resistome of the irrigated produce.

scholarly journals Degradation Sulfamethoxazole by Chlorination in Water Distribution Systems: Kinetics, Toxicity, and Antibiotic Resistance Genes

2021 ◽  
Author(s):  
Luo Xu ◽  
cong li ◽  
Guozijian Wei ◽  
Jie Ji ◽  
Eric Lichtfouse ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Distribution Systems ◽  
Water Distribution ◽  
Degradation Rate ◽  
Tap Water ◽  
Water Distribution Systems ◽  
Antibiotic Resistance Genes ◽  
Feed Additives ◽  
Batch Reactors

Abstract Sulfamethoxazole (SMX) is a veterinary drugs and feed additives, which has been frequently detected in surface waters in recent years. This paper investigated the kinetics, evolution of toxicity and antibiotic resistance genes (ARGs) of SMX in reactions with free chlorine (FC) to evaluate the fate of SMX in batch reactors and water distribution systems (WDS). In the range of investigated pH (6.3 – 9.0), the SMX degradation had the fastest rate at close to neutral pH. The chlorination of SMX could be described by the first-order kinetics, with specific second-order rate constants in batch reactors of (2.23 ± 0.07) × 102 M− s and (5.04 ± 0.30) × 101 M− s− for HClO and ClO−, respectively. And in WDS of (1.76 ± 0.07) × 102 M− s− and (4.06 ± 0.62) × 101 M− s−, respectively. The SMX degradation rate was also affected by pipe material, and the rate followed the order: stainless-steel pipe (SS) > ductile iron pipe (DI) > polyethylene pipe (PE). The flow rate from 0.7 to 1.5 m/s led to an increase of SMX degradation rate in DI, but the increase was limited. In addition, SMX could increase the toxicity of water initially, yet the toxicity reduced to the level of tap water after 2 hours chlorination. The relative abundance of ARGs (sul1 and sul2) of tap water samples was significantly increased under different conditions including only use SMX, chlorination products of SMX, or pretreatment with SMX followed by chlorination.

scholarly journals Removal of extracellular free DNA and antibiotic resistance genes from water and wastewater by membranes ranging from microfiltration to reverse osmosis

2019 ◽  
Vol 164 ◽  
pp. 114916 ◽  
Author(s):  
Katarzyna Slipko ◽  
Daniela Reif ◽  
Markus Wögerbauer ◽  
Peter Hufnagl ◽  
Jörg Krampe ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Reverse Osmosis ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Free Dna ◽  
Water And Wastewater

Profiling of intracellular and extracellular antibiotic resistance genes in tap water

2019 ◽  
Vol 365 ◽  
pp. 340-345 ◽  
Author(s):  
Han Hao ◽  
Dan-yang Shi ◽  
Dong Yang ◽  
Zhong-wei Yang ◽  
Zhi-gang Qiu ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Tap Water ◽  
Antibiotic Resistance Genes

scholarly journals Corpse Degradation of Farmed Fish Increases the Abundance of Antibiotic Resistance Genes but Exhibits the Temporal Stability

2021 ◽  
Author(s):  
Wanghong Su ◽  
Tongtong Li ◽  
Qiaoling Yu ◽  
Tianshu Feng ◽  
Jiawei Yang ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Large Volume ◽  
Tap Water ◽  
Quantitative Polymerase Chain Reaction ◽  
Temporal Stability ◽  
Antibiotic Resistance Genes ◽  
Opportunistic Pathogens ◽  
Control Groups ◽  
Farmed Fish

Abstract Serious concerns have been raised regarding resistomes causing by corpse decomposition in the aquatic environment, which pose threats to the water environment and human health. However, antibiotic resistance genes (ARGs) in large-volume tap water and their temporal stability during corpse decay are poorly explored. Here, high-throughput quantitative polymerase chain reaction (HT-qPCR) and amplicon sequencing were applied to profile ARGs and bacterial communities in experimental and control groups containing 50 L of tap water at 7th, 15th and 100th day during corpse decomposition. We found that most of the ARGs in experimental groups had higher abundance compared with the control groups independent of time. And the absolute abundance of some ARGs in the carcass groups was even enriched by 259 to 413,640-folds. The tetracycline and beta-lactamase resistance genes of the experimental groups were obviously enriched compared with control groups, and the ARG profiles were convergent during different decay stages, which indicated the long-term persistence of ARGs. Treatment, dissolved oxygen (DO) and pH were three important factors determining ARG profiles during corpse decomposition. Twelve opportunistic pathogens, especially Burkholderia, Legionella and Halomonas, remarkably increased as decomposition proceeded. Network analysis showed that opportunistic pathogens were significantly associated with ARGs. Our results emphasize that corpse decay increases the abundance and diversity of ARGs in large-volume drinking water independent of time while exhibits temporal persistence of ARGs, thereby uncovering the harmful effects of animal cadavers. It also provides valuable suggestions for the risk assessment and management of source water caused by corpse decay.

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