PKA-dependent activation of PKC, p38 MAPK and IKK in macrophage: implication in the induction of inducible nitric oxide synthase and interleukin-6 by dibutyryl cAMP

2004 ◽  
Vol 16 (5) ◽  
pp. 565-575 ◽  
Author(s):  
C Chio
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
P38 Mapk ◽  
Inducible Nitric Oxide Synthase ◽  
Interleukin 6 ◽  
Dibutyryl Camp ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

Effect of Triterpene Acids of Eriobotrya japonica (Thunb.) Lindl. Leaf and MAPK Signal Transduction Pathway on Inducible Nitric Oxide Synthase Expression in Alveolar Macrophage of Chronic Bronchitis Rats

2009 ◽  
Vol 37 (06) ◽  
pp. 1099-1111 ◽  
Author(s):  
Y. Huang ◽  
J. Li ◽  
X. M. Meng ◽  
G. L. Jiang ◽  
H. Li ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Signal Transduction ◽  
Nitric Oxide Synthase ◽  
Alveolar Macrophage ◽  
P38 Mapk ◽  
Inducible Nitric Oxide Synthase ◽  
Inos Expression ◽  
Eriobotrya Japonica ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

The goal of this study was to investigate the possible therapy mechanism of triterpene acids of Eriobotrya japonica (Thunb.) Lindl. Leaf (TAL) in alveolar macrophage (AM) of chronic bronchitis (CB) rats. CB model was established by injection of bacillus calmette guein (BCG) plus lipopolisacharide (LPS) in rats. TAL significantly inhibited the increased NO concentration, iNOS expression and phosphorylation of p38 MAPK in alveolar macrophages (AMs) of CB rats. Using in vivo test, we found that SB203580, a p38 MAPK inhibitor, (10 μM) significantly inhibited inducible nitric oxide synthase (iNOS) mRNA expression in AM. This data indicate that TAL highly decreases excessive iNOS expression and NO induction, and p38 MAPK signal transduction participates in iNOS expression and NO induction in AM of CB rats. The effect of TAL on iNOS expression in AM may be related to its inhibition of p38 MAPK signal transduction.

A germacranolide sesquiterpene lactone suppressed inducible nitric oxide synthase by downregulating NF-κB activity

2011 ◽  
Vol 89 (3) ◽  
pp. 232-237 ◽  
Author(s):  
Hwa Jin Lee ◽  
Hyeyoun Jung ◽  
Junhye Kwon ◽  
Hua Li ◽  
Da Yeon Lee ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Sesquiterpene Lactone ◽  
P38 Mapk ◽  
Inducible Nitric Oxide Synthase ◽  
Nuclear Translocation ◽  
Inos Mrna ◽  
Oxide Synthase ◽  
Signal Cascades ◽  
Inducible Nitric Oxide

A germacranolide sesquiterpene lactone, 2α,5-epoxy-5,10-dihydroxy-6α-angeloyloxy-9β-(3-methylbutyloxy)-germacran-8α,12-olide (EDAG), isolated from Carpesium triste var. manshuricum , showed inhibitory activity in the production of nitric oxide (NO) and the expression of inducible nitric oxide synthase (iNOS) mRNA and protein in LPS-activated macrophage cells. Molecular analysis reveals that these suppressive effects are correlated with the inhibition of NF-κB activation by EDAG. Immunoblotting showed that EDAG suppressed the LPS-induced degradation of I-κBα and decreased nuclear translocation of p65. Futhermore, EDAG showed reduced phosphorylation of ERK1/2 and p38 MAPK, whereas activation of JNK was not changed. These data suggest, at least in part, that EDAG utilizes the signal cascades of ERK1/2, p38 MAPK, and NF-κB for the suppression of iNOS gene expression.

scholarly journals Dual Specificity Phosphatase 1 Regulates Human Inducible Nitric Oxide Synthase Expression by p38 MAP Kinase

2011 ◽  
Vol 2011 ◽  
pp. 1-15 ◽  
Author(s):  
Tuija Turpeinen ◽  
Riina Nieminen ◽  
Ville Taimi ◽  
Taina Heittola ◽  
Outi Sareila ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
P38 Mapk ◽  
Inducible Nitric Oxide Synthase ◽  
Inos Expression ◽  
Dual Specificity Phosphatase ◽  
Dual Specificity ◽  
J774 Cells ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

The role of dual specificity phosphatase 1 (DUSP1) in inducible nitric oxide synthase (iNOS) expression in A549 human pulmonary epithelial cells, J774 mouse macrophages and primary mouse bone marrow-derived macrophages (BMMs) was investigated. iNOS expression was induced by a cytokine mixture (TNF, IFNγ and IL-1β) in A549 cells and by LPS in J774 cells, and it was inhibited by p38 MAPK inhibitors SB202190 and BIRB 796. Stimulation with cytokine mixture or LPS enhanced also DUSP1 expression. Down-regulation of DUSP1 by siRNA increased p38 MAPK phosphorylation and iNOS expression in A549 and J774 cells. In addition, LPS-induced iNOS expression was enhanced in BMMs from DUSP1(−/−)mice as compared to that in BMMs from wild-type mice. The results indicate that DUSP1 suppresses iNOS expression by limiting p38 MAPK activity in human and mouse cells. Compounds that enhance DUSP1 expression or modulate its function may be beneficial in diseases complicated with increased iNOS-mediated NO production.

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