Microwave-assisted polyethylene glycol-based aqueous two-phase extraction of gallic acid and ellagic acid from Euonymus alatus:process optimization, quantification analysis and antioxidant activity

An aqueous two-phase system of polyethylene glycol (PEG)/(NH4)2SO4 with the behavior of forming aqueous two-phase and the distribution rules of potassium glycyrrhizinate in the aqueous two-phase system were studied. Effects of the molecular weight of PEG, the mass fraction of PEG and (NH4)2SO4, and pH value were investigated. The result show that when the mass fraction of PEG1000 was 50%, the mass fraction of (NH4)2SO4 was 5.7%, and pH value was 6.5, the highest extraction recovery of potassium glycyrrhizinate could reach 99.7%. This aqueous two-phase extraction may provide a new technique for separation of potassium glycyrrhizinate.

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Separation and Enrichment of Antioxidant Peptides from Whey Protein Isolate Hydrolysate by Aqueous Two-Phase Extraction and Aqueous Two-Phase Flotation

Foods ◽

10.3390/foods8010034 ◽

2019 ◽

Vol 8 (1) ◽

pp. 34 ◽

Cited By ~ 18

Author(s):

Bin Jiang ◽

Jiaxin Na ◽

Lele Wang ◽

Dongmei Li ◽

Chunhong Liu ◽

...

Keyword(s):

Antioxidant Activity ◽

Liquid Chromatography ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Phase Extraction ◽

Scavenging Activity ◽

Antioxidant Peptides ◽

Two Phase ◽

Aqueous Two Phase Extraction

At present, peptides are separated by molecular exclusion chromatography and liquid chromatography. A separation method is needed in any case, which can be scaled up for industrial scale. In this study, aqueous two-phase extraction (ATPE) and aqueous two-phase flotation (ATPF) were applied to separate and enrich antioxidant peptides from trypsin hydrolysates of whey protein isolates (WPI). The best experimental conditions were investigated, and the results were evaluated using the 2,2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) free radical scavenging activity of the peptides-per-unit concentration and the recovery rate (Y) of peptides in the top phase of both ATPE and ATPF. Under optimal conditions, the Y and ABTS free radical scavenging activity per unit concentration in top phase of ATPE could reach 38.75% and 12.94%, respectively, and in ATPF could reach 11.71% and 29.18%, respectively. The purified peptides were characterized by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and reversed-phase high-performance liquid chromatography (RP-HPLC). PeptideCutter and PeptideMass were applied to analyze and calculate the peptide sequencing. KILDKVGINYWLAHK, VGINYWLAHKALCSEK, and TPEVDDEALEKFDKALK sequences having antioxidant activity were detected in the top phase of ATPE, and VGINYWLAHKALCSEK, KILLDKVGINYWLAHK, ILLDKVGINYWLAHK, IIAEKTKIPAVFK, KIIAEKTKIPAVFK, and VYVEELKPTPEGDLEILLQK sequences having antioxidant activity were detected in the top phase of ATPF. In conclusion, antioxidant peptides were successfully separated from the WPI hydrolysate by ATPE and ATPF; compared with ATPE, ATPF has superior specificity in separating antioxidant peptides.

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Biphasic extraction of different polysaccharides from Radix Sophorae Tonkinensis by microwave-assisted aqueous two-phase extraction: Process optimization, structural characterization and mechanism exploration

Separation and Purification Technology ◽

10.1016/j.seppur.2018.06.038 ◽

2018 ◽

Vol 207 ◽

pp. 187-198 ◽

Cited By ~ 10

Author(s):

Yuanxiang Huang ◽

Xuehao Wu ◽

Shiyu Zhou ◽

Yuyang Lin ◽

Wei Zhang ◽

...

Keyword(s):

Process Optimization ◽

Structural Characterization ◽

Extraction Process ◽

Phase Extraction ◽

Two Phase ◽

Microwave Assisted ◽

Aqueous Two Phase Extraction

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A Novel and Sensitive Method for the Determination of VitaminB2(Riboflavin) in Urine and Pharmaceutical Samples Using an Aqueous Two-Phase Extraction

Journal of Chemistry ◽

10.1155/2013/892380 ◽

2013 ◽

Vol 2013 ◽

pp. 1-5 ◽

Cited By ~ 1

Author(s):

Sabah Shiri ◽

Tahere Khezeli ◽

Sami Sajjadifar ◽

Ali Delpisheh ◽

Moayed Avazpour ◽

...

Keyword(s):

Polyethylene Glycol ◽

Standard Deviation ◽

Limit Of Detection ◽

Vitamin B ◽

Phase Extraction ◽

Two Phase ◽

Pharmaceutical Samples ◽

Relative Standard ◽

Aqueous Two Phase Extraction

A novel, simple, and more sensitive spectrophotometric procedure has been developed for the determination of vitamin B2(riboflavin) by an aqueous two-phase extraction (ATPE). An ATPE is formed mostly by water and does not require an organic solvent. Other ATPE components used in this study were the polymer, polyethylene glycol (PEG), and some salts such as Na2SO4and Na2CO3. The method is based on the interaction between vitamin B2(riboflavin) and sodium sulfate (Na2SO4) in an acidic medium (pH 6.4). The influences of effective parameters such as salt (type and concentration), polyethylene glycol (molecular weight and concentration), temperature, centrifuging time, and pH of the sample solution were studied and optimized. The linear range was 1.3–320 ng/mL (R2=0.9991;n=10) with the relative standard deviation (RSD) for 60 ng/mL 3.68%. The limit of detection (LOD) calculated from three times of standard deviation of blank were 0.2 ng/mL and recoveries from analysis of real samples between 94.82% and 103.98% were obtained for the determination of vitamin B2(riboflavin) in urine and pharmaceutical samples.

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Extraction and characterization of polysaccharides from Semen Cassiae by microwave-assisted aqueous two-phase extraction coupled with spectroscopy and HPLC

Carbohydrate Polymers ◽

10.1016/j.carbpol.2016.02.063 ◽

2016 ◽

Vol 144 ◽

pp. 263-270 ◽

Cited By ~ 42

Author(s):

Zhi Chen ◽

Wei Zhang ◽

Xunyou Tang ◽

Huajun Fan ◽

Xiujuan Xie ◽

...

Keyword(s):

Phase Extraction ◽

Two Phase ◽

Microwave Assisted ◽

Aqueous Two Phase Extraction

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Hyphenation of aqueous two-phase and microwave extraction of solasonine and solamargine from leaves of Solanum mauritianum characterized by UHPLC-qTOF-MS

Biomass Conversion and Biorefinery ◽

10.1007/s13399-021-02136-y ◽

2021 ◽

Author(s):

Tebogo Mphatlalala Mokgehle ◽

Ntakadzeni Edwin Madala ◽

Wilson Mugera Gitari ◽

Nikita Tawanda Tavengwa

Keyword(s):

Phase Extraction ◽

Microwave Assisted Extraction ◽

Two Phase ◽

One Pot ◽

Invasive Weed ◽

Microwave Assisted ◽

Assisted Extraction ◽

Solanum Mauritianum ◽

The One ◽

Aqueous Two Phase Extraction

AbstractThe biomass Solanum mauritianum (S. mauritianum) is an invasive weed specie; however, it is a source of medicinally important metabolites, as reported in literature, such as solasonine and solamargine. The study was directed at the optimization of microwave and aqueous two-phase-based extraction techniques which involved microwave-assisted extraction (MAE), aqueous two-phase extraction followed by microwave-assisted extraction (ATPE + MAE), and the “one-pot” microwave-assisted aqueous two-phase extraction (MA-ATPE) for extraction of solasonine and solamargine from leaves of S. mauritianum which was evaluated. The microwave-assisted extraction of solasonine and solamargine yielded optimums at 5.00 min, microwave power of 270 W, and solid/liquid of 0.1 g L−1 at an ethanol concentration of 60%. Application of a two-stage extraction (MAE + ATPE) in CaO-dried alcohol resulted in decreased amounts of solasonine and solamargine extracted. The best yields of solasonine and solamargine were achieved in the MA-ATPE method. Extraction of solamargine and solasonine using Na2CO3 in CaO-dried ethanol during MA-ATPE was approximately threefold and twofold greater than that of MAE + ATPE, respectively. Furthermore, extraction of solamargine and solasonine using NaCl in CaO-dried ethanol during MA-ATPE was approximately twofold greater than that of MAE + ATPE. The synergy of microwaves and salting-out in the “one-pot” MA-ATPE technique was shown to be a contributing factor for enhanced extraction of solamargine and solasonine from leaves of S. mauritianum. Application of this time- and energy-efficient extraction method could potentially be expanded for enrichment of medicinally important compounds from biomass of other medicinal plants.

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