Monitoring microbial community in a subsurface soil contaminated with hydrocarbons by quinone profile

Chemosphere ◽  
2005 ◽  
Vol 59 (3) ◽  
pp. 305-314 ◽  
Author(s):  
Dejun Song ◽  
Arata Katayama
Keyword(s):  
Microbial Community ◽  
Quinone Profile ◽  
Subsurface Soil

Effects of biodegradable substrates and microbial concentration on the acclimation of microbes to acrylonitrile in aerobic submerged biofilter

1998 ◽  
Vol 38 (7) ◽  
pp. 81-89 ◽  
Author(s):  
Hong-Ying Hu ◽  
Koichi Fujie ◽  
Mamie Nozawa ◽  
Tsuyoshi Makabe ◽  
Kohei Urano
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Microbial Community ◽  
Community Change ◽  
Ecological Studies ◽  
Profile Method ◽  
Upper Limit ◽  
Quinone Profile ◽  
Microbial Film ◽  
Corynebacterium Sp ◽  
Complete Mineralization

The effects of coexistence-of biodegradable substrates and microbial concentration on the acclimation of microbes to acrylonitrile (AN) in an aerobic submerged biofilter were investigated on the basis of kinetic and ecological studies. The experimental results show that the acclimation of microbial film to AN was promoted by a higher microbial concentration in the biofilter and by the coexistence of glucose and peptone in the influent. It was clarified that the upper limit of AN loading to the biofilter for the ultimate degradation, i.e., complete mineralization, of the influent AN was about 2.0-2.2 kg/m3. d. In addition, a new microbial quinone profile method was applied for the analysis of the microbial community change in the biofilter. The change in quinone profiles of the microbial film during the acclimation to AN suggested that Brevibacterium sp., Pseudomonas aeruginosa and Corynebacterium sp. could contribute to the degradation of AN in the aerobic biofilter.

The stability of nitrite nitrification with strong nitrogenous wastewater: effects of organic concentration and microbial diversity

2004 ◽  
Vol 49 (5-6) ◽  
pp. 89-96 ◽  
Author(s):  
Z. Yun ◽  
Y.-H. Jung ◽  
B.-R. Lim ◽  
E. Choi ◽  
K.S. Min
Keyword(s):  
Experimental Study ◽  
Microbial Community ◽  
Profile Analysis ◽  
Control Unit ◽  
Operating Period ◽  
Ammonia Inhibition ◽  
Start Up ◽  
Quinone Profile ◽  
Competition Hypothesis ◽  
The Stability

A stable achievement of nitritation with strong nitrogenous wastewaters is considered as a difficult task in practice, probably due to the fate of interaction between dominating heterotrophs and nitrifier species. An experimental study was carried out to examine the organic effects in lab-scale biofilm nitritation reactors. The control unit without organic addition showed a stable nitritation performance for more than 220 days of operating period. The nitritation activity gradually failed at the reactors with an organic addition, but the nitritation activity eventually recovered with a prolonged aeration. It was not possible to explain the nitritation recovery with neither free ammonia inhibition concept nor DO competition hypothesis in these cases. The results suggest that the nitritation with organic requires a long start-up period for acclimation. In addition, the results of quinone profile analysis were in agreement with nitritation activity in reactors. The diversity of microbial community in the nitritation reactors could be described by the quinone profiles.

scholarly journals Supercritical Fluid Extraction of Quinones from Compost for Microbial Community Analysis

2015 ◽  
Vol 2015 ◽  
pp. 1-7
Author(s):  
Ni Luh Gede Ratna Juliasih ◽  
Lee Chang Yuan ◽  
Yuki Sago ◽  
Yoichi Atsuta ◽  
Hiroyuki Daimon
Keyword(s):  
Microbial Community ◽  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Community Dynamics ◽  
Community Analysis ◽  
Ultra Performance Liquid Chromatography ◽  
Microbial Community Analysis ◽  
Fluid Extraction ◽  
Dissimilarity Index ◽  
Quinone Profile

Supercritical fluid extraction (SFE) was used to extract quinones from compost to monitor the microbial community dynamics during composting. The 0.3 g of dried compost was extracted using 3 mL min−1of carbon dioxide (90%) and methanol (10%) at 45°C and 25 MPa for a 30 min extraction time. The extracted quinones were analysed using ultra performance liquid chromatography (UPLC) with 0.3 mL min−1of methanol mobile phase for a 50 min chromatographic run time. A comparable detected amount of quinones was obtained using the developed method and an organic solvent extraction method, being 36.06 μmol kg−1and 34.54 μmol kg−1, respectively. Significantly low value of dissimilarity index (D) between the two methods (0.05) indicated that the quinone profile obtained by both methods was considered identical. The developed method was then applied to determine the maturity of the compost by monitoring the change of quinone during composting. The UQ-9 and MK-7 were predominant quinones in the initial stage of composting. The diversity of quinone became more complex during the cooling and maturation stages. This study showed that SFE had successfully extracted quinones from a complex matrix with simplification and rapidity of the analysis that is beneficial for routine analysis.

Change of microbial community structure by respiratory quinone profile at intermittently aerated membrane bioreactor

2004 ◽  
Vol 49 (5-6) ◽  
pp. 459-465 ◽  
Author(s):  
P. Songprasert ◽  
B.-R. Lim ◽  
K.-H. Ahn
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
Membrane Bioreactor ◽  
Domestic Wastewater ◽  
Time Interval ◽  
Respiratory Quinone ◽  
Aerobic Conditions ◽  
Submerged Membrane Bioreactor ◽  
Quinone Profile

In the present study, the microbial community structure in an intermittently aerated submerged membrane bioreactor treating domestic wastewater was observed using the respiratory quinone profiles. The effects of different time interval for the aerobic and anoxic period on microbial community structure were examined with 60/90 min (Step 1) and 90/60 min (Step 2) as anoxic/aerobic periods. There was an observable slight difference in microbial community structure between Step 1 and Step 2 in the submerged membrane bioreactor. The dominant quinone types for Step 1 at both anoxic and aerobic conditions were UQ-8 followed by UQ-10 and MK-6, but those for Step 2 were UQ-8, MK-6 and MK-10(H4). The microbial diversity of Step 1 and Step 2 based on the composition of all quinones was 10.6Ð11.7 and 13.3Ð13.0 for anoxic and aerobic conditions, respectively. The present results suggest that the introduction of intermittent aeration into the submerged membrane bioreactor has little influence on the bacterial community structure.

Microbial community in biofilm on membrane surface of submerged MBR: effect of in-line cleaning chemical agent

2005 ◽  
Vol 51 (6-7) ◽  
pp. 201-207 ◽  
Author(s):  
B.-R. Lim ◽  
K.-H. Ahn ◽  
K.-G. Song ◽  
J.W. Cho
Keyword(s):  
Microbial Community ◽  
Membrane Surface ◽  
Cell Lysis ◽  
Chemical Agent ◽  
Chemical Cleaning ◽  
Organic Degradation ◽  
Community Pattern ◽  
Cleaning Agent ◽  
Quinone Profile

The objective of this study was to investigate the change in microbial community pattern with the effect of cleaning agent using a quinone profile that is used for membrane in-line chemical cleaning in SMBR. The dominant quinone types of biofilm were ubiquinone (UQs)-8, -10, followed by menaquinone (MKs)-8(H4), -7 and UQ-9, but those of suspended microorganisms were UQ-8, UQ-10 followed by MKs-8(H4), -7 and -11. Both UQ and MK contents decreased with increasing NaClO dosage and it seems that there is more resistance from UQ compared to MK. In addition, COD and DOC concentrations increased with increasing NaClO dosage up to 0.05 g-NaClO/g-SS. The organic degradation performance of the microbial community in the presence of NaClO was impaired. The present study suggested that larger added amounts of NaClO caused an inhibition of organic degradation and cell lysis.

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