This study developed an oxygenase system with horizontal-axis wind turbine driving the oxygenation device by belt pulley for aquaculture, and verified the feasibility of the system in conditions of Taiwan’s average wind speed. The experimental system is consisted of a horizontal wind turbine, a reciprocating compressor, and water channels. At the first stage of the experiment, the reciprocating compressor oxygenase system was measured according to the power supply standards in terms of power consumption, air displacement and oxygen production, in case of various rotating speeds and the compliance with aquaculture standards. At the second stage of the experiment, the wind turbine was used to directly drive the reciprocating compressor oxygenase system. According to the experimental results, regarding the test of the compressor oxygenase system, when power supply rotating speed is 406.7 rpm, power consumption is 234.5 W and the oxygen production is 7.48mg/L, which is above the level of amount of dissolved oxygen of aquaculture at 5.5mg/L. In case of driving the oxygenation device by wind power, when wind speed is 5.06 m/s and the wind turbine rotating speed is 140 rpm, the average dissolved oxygen in the water is 5.9 mg/L, which meets the aquaculture standards. Even in case of unstable wind speed, good oxygen production effects can be achieved. Moreover, the system is directly driven by wind power and does not require electric power.