Utilization of starch effluent from a textile industry as a fungal growth supplement for enhanced α-amylase production for industrial application

Chemosphere ◽  
2021 ◽  
pp. 130554
Author(s):  
Shweta Kalia ◽  
Arghya Bhattacharya ◽  
Sanjeev Kumar Prajapati ◽  
Anushree Malik

Enzyme technology is extensively touted as the way of the destiny for textile processing industry. Enzymes can be used safely in a wide selection of textile processes such as de-sizing, scouring, bleaching, dyeing, and finishing in textile processing. Recently, amylase enzyme is getting more important in textile industry for removal of starch. Amylase can be successfully applied at early pre-treatment stages, making a sturdy basis for the good finishing of fabrics. The present study was focused to isolate amylase producing bacteria and optimize the growth condition for maximizing amylase production using agro-industrial residues. In addition, immense important for RSM based optimization for the process parameters, pH, temperature, and agitation speed also given. Further, this study concentrated for determination of desizing potency of Denim by the amylase that produced at optimized condition.


2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Shalini Singh ◽  
Sanamdeep Singh ◽  
Vrinda Bali ◽  
Lovleen Sharma ◽  
Jyoti Mangla

The study aimed at isolation and screening of fungal amylase producer, optimization of solid state fermentation conditions for maximum amylase production by the best amylase producer, and characterization of the crude amylases, so produced.Aspergillus fumigatusNTCC1222 showed the highest amylase activity (164.1 U/mL) in secondary screening under SSF conditions and was selected for further studies. The test strain showed maximum amylase production (341.7 U/mL) and supernatant protein concentration (9.7 mg/mL) for incubation period (6 days), temperature (35°C), initial pH (6.0), nutrient salt solution as moistening agent, and beef extract as nitrogen source. Pomegranate peel produced maximum amylase activity, but wheat bran (only slightly lesser amylase activity as compared to that of pomegranate peel) was chosen for further studies, keeping in mind the seasonal availability of pomegranate peel. TLC confirmed the amylase produced to beα-type and 60 kDa was the molecular weight of the partially purified amylase. The enzyme showed maximum enzyme activity at pH 6.0, temperature of 55°C, and incubation time of 60 minutes. UV (616.0 U/mL) and chemical (814.2 U/mL) mutation enhanced amylase activity as compared to wild test strain. The study indicates thatAspergillus fumigatusNTCC1222 can be an important source of amylase and the crude enzyme, hence obtained, can be cost effectively applied in multiple sections of textile wet processing.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Aleksandra Góralczyk-Bińkowska ◽  
Andrzej Długoński ◽  
Przemysław Bernat ◽  
Jerzy Długoński ◽  
Anna Jasińska

AbstractTextile industry effluents and landfill leachate contain chemicals such as dyes, heavy metals and aromatic amines characterized by their mutagenicity, cytotoxicity and carcinogenicity. The aim of the present study was investigation of the ascomycete fungus N. pironii isolated from urban postindustrial textile green space for its ability to grow and retain metabolic activity in the presence of the dye industry waste. Research focused mainly on dyes, heavy metals and aromatic amines, which had been detected in landfill leachate via HPLC–MS/MS analysis. Presence of all tested compounds as well as leachate in the growth medium clearly favored the growth of fungal biomass. Only slight growth limitation was observed in the presence of 50 mg L-1o-tolidine. The fungus eliminated o-tolidine as well as dyes at all tested concentrations. The presence of metals slightly influenced the decolorization of the azo dyes; however, it was still similar to 90%. During fungal growth, o-tolidine was hydroxylated and/or converted to toluidine and its derivatives. Laccase and cytochrome P450 involvement in this process has been revealed. The results presented in the paper provide a valuable background for the development of a fungus-based system for the elimination of toxic pollutants generated by the textile industry.


Author(s):  
H.M. Mazzone ◽  
G. Wray ◽  
R. Zerillo

The fungal pathogen of the Dutch elm disease (DED), Ceratocystis ulmi (Buisman) C. Moreau, has eluded effective control since its introduction in the United States more than sixty years ago. Our studies on DED include establishing biological control agents against C. ulmi. In this report we describe the inhibitory action of the antibiotic polymyxin B on the causal agent of DED.In screening a number of antibiotics against C. ulmi, we observed that filter paper discs containing 300 units (U) of polymyxin B (Difco Laboratories) per disc, produced zones of inhibition to the fungus grown on potato dextrose agar or Sabouraud agar plates (100mm x 15mm), Fig. 1a. Total inhibition of fungal growth on a plate occurred when agar overlays containing fungus and antibiotic (polymyxin B sulfate, ICN Pharmaceuticals, Inc.) were poured on the underlying agar growth medium. The agar overlays consisted of the following: 4.5 ml of 0.7% agar, 0.5 ml of fungus (control plate); 4.0 ml of 0.7% agar, 0.5 ml of fungus, 0.5 ml of polymyxin B sulfate (77,700 U). Fig. 1, b and c, compares a control plate and polymyxin plate after seven days.


1910 ◽  
Vol 103 (19) ◽  
pp. 358-358
Author(s):  
Arthur H. J. Keane
Keyword(s):  

2020 ◽  
Vol 21 (6) ◽  
pp. 610
Author(s):  
Xiaoliang Cheng ◽  
Chunyang Zhao ◽  
Hailong Wang ◽  
Yang Wang ◽  
Zhenlong Wang

Microwave cutting glass and ceramics based on thermal controlled fracture method has gained much attention recently for its advantages in lower energy-consumption and higher efficiency than conventional processing method. However, the irregular crack-propagation is problematic in this procedure, which hinders the industrial application of this advanced technology. In this study, the irregular crack-propagation is summarized as the unstable propagation in the initial stage, the deviated propagation in the middle stage, and the non-penetrating propagation in the end segment based on experimental work. Method for predicting the unstable propagation in the initial stage has been developed by combining analytical models with thermal-fracture simulation. Experimental results show good agreement with the prediction results, and the relative deviation between them can be <5% in cutting of some ceramics. The mechanism of deviated propagation and the non-penetrating propagation have been revealed by simulation and theoretical analysis. Since this study provides effective methods to predict unstable crack-propagation in the initial stage and understand the irregular propagation mechanism in the whole crack-propagation stage in microwave cutting ceramics, it is of great significance to the industrial application of thermal controlled fracture method for cutting ceramic materials using microwave.


2016 ◽  
Vol 6 (2) ◽  
pp. 219-239
Author(s):  
Olgu Karan

This paper proposes a new conceptual framework in understanding the dynamics within the Kurdish and Turkish (KT) owned firms in London by utilising Charles Tilly’s work concerning collective resource mobilisation. Drawing on 60 in-depth interviews with restaurant, off-licence, kebab-shop, coffee-shop, supermarket, wholesaler owners and various community organisations, the paper sheds light upon the questions of why and how the KT communities in London moved into, and are over represented and why Turkish Cypriots are absent in small business ownership. The re-search illustrates that members of the KT communities aligned in their interests to become small business owners after the demise of textile industry in the midst of 1990s in London. The interest alignment in small business ownership required activation of various forms of capital and transposition of social, cultural and economic capital into one another.


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