Two-dimensional capillary liquid chromatography: pH Gradient ion exchange and reversed phase chromatography for rapid separation of proteins

2006 ◽  
Vol 1120 (1-2) ◽  
pp. 132-141 ◽  
Author(s):  
Milaim Pepaj ◽  
Steven R. Wilson ◽  
Katerina Novotna ◽  
Elsa Lundanes ◽  
Tyge Greibrokk
Keyword(s):  
Liquid Chromatography ◽  
Ion Exchange ◽  
Reversed Phase ◽  
Capillary Liquid Chromatography ◽  
Ph Gradient ◽  
Two Dimensional ◽  
Rapid Separation ◽  
Reversed Phase Chromatography ◽  
Separation Of Proteins ◽  
Capillary Liquid

Separation of Liquiritin by Two-Dimensional Liquid Chromatography

2012 ◽  
Vol 455-456 ◽  
pp. 1232-1238 ◽  
Author(s):  
Jing Xiang Cong ◽  
Shao Yan Wang ◽  
Hong Gao
Keyword(s):  
Liquid Chromatography ◽  
Reversed Phase ◽  
Normal Phase ◽  
Size Exclusion ◽  
Two Dimensional ◽  
Target Compound ◽  
Reversed Phase Chromatography ◽  
Complex Samples ◽  
Exclusion Chromatography ◽  
Licorice Extract

Two-dimensional liquid chromatography (2DLC) is an important technology for the separation and analysis of complex samples. Liquiritin, an important active component in licorice, was chosen as the target compound and it was separated by three kinds of off-line 2DLC, i.e. size exclusion chromatography × reversed phase chromatography, normal phase × reversed phase chromatography and reversed phase chromatography × reversed phase chromatography (SEC×RP, NP×RP and RP×RP). The chromatographic conditions were selected and the 2D systems were combined. The results show that it is feasible to separate Liquiritin from licorice extract using 2DLC. Among the 2D modes mentioned above, the highest purity of Liquiritin was obtained in the RP×RP mode, and the concentration of Liquiritin was increased most significantly in the NP×RP mode.

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