Iron Oxide Nanoparticles (IONPs) with potential applications in plasmid DNA isolation

Author(s):  
J.R Sosa-Acosta ◽  
J.A. Silva ◽  
L. Fernández-Izquierdo ◽  
S. Díaz-Castañón ◽  
M. Ortiz ◽  
...  
RSC Advances ◽  
2016 ◽  
Vol 6 (112) ◽  
pp. 111099-111108 ◽  
Author(s):  
Nikesh Gupta ◽  
Chetna Gupta ◽  
Sandeep Sharma ◽  
Brijesh Rathi ◽  
Rakesh Kumar Sharma ◽  
...  

We report synthesis of iron oxide nanoparticles encapsulating HRP. The average diameter of the particles was around 20 nm. HRP has been used to convert IAA to a toxic oxidized product and its toxic effect has been seen on cancerous cell lines.


2016 ◽  
Vol 14 (1) ◽  
Author(s):  
Luisa H. A. Silva ◽  
Jaqueline R. da Silva ◽  
Guilherme A. Ferreira ◽  
Renata C. Silva ◽  
Emilia C. D. Lima ◽  
...  

2021 ◽  
Author(s):  
Nguyen Hoang Nam

Multifunctional silver nanoparticles have attracted widely due to their potential applications. Based on the properties of individual silver nanoparticles, such as plasmonic and antibacterial properties, silver nanoparticles can become multifunctional by surface modifications with various surfactants or they can be combined in core-shell and composite structures with the magnetic nanoparticles to form bifunctional nanoparticles. After reviewing the methods of synthesis and applications of silver nanoparticles, the chapter describes the synthesis and the properties of the new types of multifunctional silver nanomaterials based on the plasmonic behaviors of silver nanoparticles and the iron oxide Fe3O4 superparamagnetic nanoparticles. One type is a simple combination of silver nanoparticles and iron oxide nanoparticles in a silica matrix Fe3O4/Ag-4ATP@SiO2. Other types are the core-shell structured nanoparticles, where Fe3O4 nanoparticles play as the core and silver nanoparticles are the outer shell, so-called Fe3O4@SiO2-Ag and Fe3O4-Ag. In the Fe3O4@SiO2-Ag, silver nanoparticles are reduced on the surface of silica-coated magnetic core, while in Fe3O4-Ag, silver nanoparticles are directly reduced on the amino groups functionalized on the surface of magnetic nanoparticles without coating with silica. Both of types of the multifunctional silver nanoparticles show the plasmonic and magnetic properties similar as the individual silver and iron oxide nanoparticles. Finally, some applications of those multifunctional silver nanoparticles will be discussed.


Nanophotonics ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 2321-2329 ◽  
Author(s):  
Song Zhu ◽  
Lei Shi ◽  
Linhao Ren ◽  
Yanjing Zhao ◽  
Bo Jiang ◽  
...  

AbstractOptical frequency comb (OFC) based on the whispering-gallery-mode microresonator has various potential applications in fundamental and applied areas. Once the solid microresonator is fabricated, its structure parameters are generally unchanged. Therefore, realizing the tunability of the microresonator OFC is an important precondition for many applications. In this work, we proposed and demonstrated the tunable Kerr and Raman-Kerr frequency combs using the ultrahigh-quality-factor (Q) functionalized silica microsphere resonators, which are coated with iron oxide nanoparticles on their end surfaces. The functionalized microsphere resonator possesses Q factors over 108 and large all-optical tunability due to the excellent photothermal performance of the iron oxide nanoparticles. We realized a Kerr frequency comb with an ultralow threshold of 0.42 mW and a comb line tuning range of 0.8 nm by feeding the control light into the hybrid microsphere resonator through its fiber stem. Furthermore, in order to broaden the comb span, we realized a Raman-Kerr frequency comb with a span of about 164 nm. Meanwhile, we also obtained a comb line tuning range of 2.67 nm for the Raman-Kerr frequency comb. This work could find potential applications in wavelength-division multiplexed coherent communications and optical frequency synthesis.


2021 ◽  
Vol 12 ◽  
Author(s):  
Mark Geppert ◽  
Martin Himly

Iron oxide nanoparticles (IONPs) bear big hopes in nanomedicine due to their (potential) applications in tumor therapy, drug delivery or bioimaging. However, as foreign entities, such particles may be recognized by the immune system and, thus, lead to inflammation, hypersensitivity or anaphylactic shock. In addition, an overload with iron is known to cause oxidative stress. In this short review, we summarize the biological effects of such particles with a major focus on IONP-formulations used for bioimaging purposes and their effects on the human immune system. We conclude that especially the characteristics of the particles (size, shape, surface charge, coating, etc.) as well as the presence of bystander substances, such as bacterial endotoxin are important factors determining the resulting biological and immunological effects of IONPs. Further studies are needed in order to establish clear structure-activity relationships.


2021 ◽  
Author(s):  
bahar Nikforouz ◽  
alireza allafchian ◽  
Seyed Amir Hossein Jalali ◽  
Hamideh Shakeripour ◽  
Rezvan Mohammadinezhad

Abstract This study investigates the potential of iron oxide nanoparticles (Fe3O4) and quince seed mucilage (QSM) as combined genetic carriers to deliver plasmid DNA (pDNA) through the gastrointestinal system. The samples are characterized by XRD, zeta potential, DLS, FT-IR spectroscopy, FE-SEM and VSM. The stability of pDNA loading on the nanocarriers and their release pattern are evaluated in simulated gastrointestinal environments by electrophoresis. The XRD patterns reveal that the nanocarriers could preserve their structure during various synthesis levels. The saturation magnetization (Ms) of the Fe3O4 cores are 56.48 emu/g without any magnetic hysteresis. Not only does the loaded pDNA contents experience a remarkable stability in the simulated gastric environment, but also, they could be released up to 99% when exposed to an alkaline environment similar to the intestinal fluid of fish. The results indicate that the synthesized nanoparticles could be employed as efficient low-cost pDNA carriers.


2020 ◽  
Vol 1 (1) ◽  
pp. 22-30
Author(s):  
Tanzila Sahar ◽  
Huma Munir ◽  
Zahida Zia ◽  
Nageen ◽  
Naila Rafiq ◽  
...  

Green protocols being eco-friendly and cost effective approach are most widely used for the production of iron oxide nanoparticles using plant-mediated extract of Citrus Sinensis, moreoverbiosynthesized iron oxide (FeO) nanoparticles shows better antibacterial activity.Green synthesis of nanoparticles has been broadly studied from the past few years because of their different features and potential applications in various fields. The successful biosynthesis of iron oxide nanoparticles was confirmed and characterized using UV-Visible spectroscopy, Scanning Electron Microscope (SEM), Fourier Transform Infrared (FTIR) analysis and Zeta sizer. Antibacterial effect of biologically produced iron oxide nanoparticles was tested against Gram-negative bacteria (Escherichia coli) and Gram-positive bacteria (Macrococus). These results exhibited that iron oxide nanoparticles have high antibacterial potential as these nanoparticles showed significant zone of inhibition against bacteria strains. The proposed green synthesis of iron oxide nanoparticles (NPs) from Citrus Sinensis can be strongly recommended as a potential method for industrial application.


Materials ◽  
2021 ◽  
Vol 14 (24) ◽  
pp. 7652
Author(s):  
Sylwia Fiejdasz ◽  
Adriana Gilarska ◽  
Tomasz Strączek ◽  
Maria Nowakowska ◽  
Czesław Kapusta

The paper presents results of our studies on hybrid materials based on polymers of natural origin containing superparamagnetic iron oxide nanoparticles (SPIONs). Such nanoparticles, coated with the chitosan derivative, were immobilized in a chitosan-collagen hydrogel matrix by crosslinking with genipin. Three types of biopolymer matrices of different collagen-to-chitosan ratios were studied. A thorough magnetic characterization was performed, including magnetic susceptibility, magnetization, and hysteresis loop measurements in a temperature range of 4 K to 300 K and a magnetic field induction up to 8 Tesla. The effect of SPION immobilization and material composition on the magnetic properties of the hybrids was investigated. The results showed that hybrid materials with covalently bounded SPIONs preserved the superparamagnetic character of SPIONs and exhibited promising magnetic properties, which are important for their potential applications.


2018 ◽  
Vol 5 (12) ◽  
pp. 181369
Author(s):  
Adheesha N. Danthanarayana ◽  
Danushika C. Manatunga ◽  
Rohini M. De Silva ◽  
N. Vishvanath Chandrasekharan ◽  
K. M. Nalin De Silva

This study was carried out to develop a simple and efficient method to isolate DNA directly from biological samples using iron oxide nanoparticles (IONPs) functionalized with polyethyleneimine (PEI). IONPs were synthesized via co-precipitation method followed with direct attachment of branched PEI. Nanoparticles were characterized using STEM, FT-IR spectroscopy and XRD analysis. The binding capacity of synthesized PEI-IONPs for plasmid and genomic DNA was assessed using purified DNA samples. In order to elute bound DNA, elution conditions were optimized, changing pH, salt concentration and temperature. Synthesized PEI-IONPs were subjected to isolation of DNA from bacterial cell culture and from human blood. PCR and magnetofection of the enhanced green fluorescence protein (EGFP) were carried out to verify the downstream applications of isolated DNA. The results indicated that the synthesized nanoparticles were of 5–10 nm. The binding capacity of PEI-IONPs for plasmid DNA and genomic DNA were 5.4 and 8.4 µg mg −1 , respectively, which were even higher than the commercially available kits such as Mag-bind, MagJET and Magmax. The optimized condition for plasmid DNA elution was 0.1 M Tris HCl (pH 10.0), 1.5 M NaCl and 5% formamide, maintained at the temperature of 60°C. The optimized condition for genomic DNA elution was 0.1 M Tris HCl (pH 10.0), 1.5 M NaCl and 10% formamide, maintained at 60°C. PCR and magnetofection processes were successful. This study revealed that the magnetic separation of DNA using PEI-IONPs is a simple and efficient method for direct isolation of DNA from biological samples which can be then used in various downstream applications.


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