Background: Nanoparticles (iron oxide and titanium dioxide nanoparticles) are another kind of critical materials that are produced for use in various research and different purposes. The bacteriology field being so critical seek to the intrinsic understanding on the effect of nanoparticles on bacterial growth and functions. Our investigation was planned to detect the impact of iron oxide (Fe3O4), titanium dioxide (TiO2) nanoparticles on growth of Escherichia coli (Iraqi isolate).
Methods: Fifty urine samples of patients, who are suffering Urinary Tract Infections (UTIs) in Iraqi hospitals, were collected. Our study was included three parts: the 1st part was isolated and diagnosed the bacteria that cause the urinary tract infection, the 2nd part was sensitivity to antibiotics, and the 3rd has used the nanomaterials and study their impacts on the growth of E. col isolates.
Result: The results showed that 30 E. coli isolates depending on the properties of biochemical and molecular detect. Five common types of antibiotics were examined for the treatment of infections of the urinary tract. Most E. coli were resistant to antibiotics, the ratios of ampicillin, amikacin and augmentin found to be 90%, 82% and 80% respectively. It concluded that bacteria were sensitive to imipenem and meropenem of about 50 %. So, the effect of iron oxide and titanium dioxide nanoparticles were studied for the growth of bacteria using the agar. The effectiveness against bacteria (diameters of the inhibition zone rate) found to be 18 mm for the 1st substance and 21 mm for 2nd substance.
Conclusion: Our current study indicates that there is an effect of nanoparticles at the cellular level that can be used for beneficial biological application such as antibacterial.
Keywords: Escherichia coli; Inhibition zone; Antibiotics; Nanoparticles
Metabolomic and proteomic investigations of impacts of titanium dioxide nanoparticles on Escherichia coli
