Direct mecA polymerase chain reaction testing of blood culture bottles growing Gram-positive cocci and the clinical potential in optimizing antibiotic therapy for staphylococcal bacteremia

2013 ◽  
Vol 75 (1) ◽  
pp. 37-41 ◽  
Author(s):  
Bing Wang ◽  
Peter Jessamine ◽  
Marc Desjardins ◽  
Baldwin Toye ◽  
Karam Ramotar
Keyword(s):  
Polymerase Chain Reaction ◽  
Blood Culture ◽  
Antibiotic Therapy ◽  
Chain Reaction ◽  
Gram Positive ◽  
Polymerase Chain Reaction Testing ◽  
Polymerase Chain ◽  
Clinical Potential ◽  
Gram Positive Cocci

scholarly journals Blood Culture–Negative Cardiovascular Infection in a Patient With Multiple Sclerosis

2019 ◽  
Vol 6 (10) ◽  
Author(s):  
Cléa Melenotte ◽  
Ahmed Loukil ◽  
Audrey Rico ◽  
Hubert Lepidi ◽  
Didier Raoult
Keyword(s):  
Multiple Sclerosis ◽  
Polymerase Chain Reaction ◽  
Blood Culture ◽  
Chain Reaction ◽  
Serological Tests ◽  
Specific Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Testing ◽  
Polymerase Chain ◽  
Culture Negative

Abstract A patient with multiple sclerosis presented with seronegative C. burnetii endocarditis diagnosed using C. burnetii–specific polymerase chain reaction and fluorescence in situ hybridization on cardiovascular biopsy. This case supports the necessity of a systematic polymerase chain reaction testing of removed cardiac valves because blood culture–negative endocarditis can be pauci-symptomatic, and serological tests can be negative in cases of immunosuppression.

scholarly journals Validation of polymerase chain reaction in experimental sepsis diagnosis beyond blood culture

Critical Care ◽  
2008 ◽  
Vol 12 (Suppl 5) ◽  
pp. P47
Author(s):  
Marcello Ruiz-Silva ◽  
Derci Sa-Filho ◽  
Marcos Caseiro ◽  
Ivan Koh
Keyword(s):  
Polymerase Chain Reaction ◽  
Blood Culture ◽  
Experimental Sepsis ◽  
Chain Reaction ◽  
Sepsis Diagnosis ◽  
Polymerase Chain

Persistent Leptospiruria in Five Dogs Despite Antimicrobial Treatment (2000–2017)

2019 ◽  
Vol 55 (1) ◽  
pp. 42-47 ◽  
Author(s):  
Tara Mauro ◽  
Kenneth Harkin
Keyword(s):  
At Risk ◽  
Polymerase Chain Reaction ◽  
Antimicrobial Treatment ◽  
Renal Tubules ◽  
Chain Reaction ◽  
Polymerase Chain Reaction Testing ◽  
Pet Owners ◽  
Zoonotic Risk ◽  
The Face ◽  
Polymerase Chain

ABSTRACT In dogs with leptospirosis, doxycycline therapy is recommended as the preferred therapy for its ability to eliminate the organism from all tissues, including the renal tubules. Elimination of organisms from the renal tubules terminates leptospiruria and prevents transmission of the organism. This report describes the discovery of persistent leptospiruria in the face of therapy with doxycycline in four dogs and enrofloxacin in one dog. Leptospiruria was confirmed by polymerase chain reaction testing for pathogenic leptospires in all five dogs. In two dogs, leptospiruria resolved after a change in therapy to enrofloxacin. In three dogs, doxycycline and/or enrofloxacin were ineffective at eliminating leptospiruria, which then resolved after therapy with clarithromycin. Pet owners could be at risk as persistent leptospiruria poses a potential zoonotic risk. The potential reasons for persistent leptospiruria as demonstrated by polymerase chain reaction testing are discussed.

scholarly journals Impact of Multiplex Polymerase Chain Reaction Testing for Respiratory Pathogen Detection in Pediatric Patients

2017 ◽  
Vol 4 (suppl_1) ◽  
pp. S503-S503
Author(s):  
Courtney C Sutton ◽  
Patti J Walton ◽  
Montgomery F Williams ◽  
Tracey L Bastian ◽  
Michael Wright ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Polymerase Chain Reaction ◽  
Pathogen Detection ◽  
Pediatric Patients ◽  
Respiratory Pathogen ◽  
Chain Reaction ◽  
Polymerase Chain Reaction Testing ◽  
Polymerase Chain

scholarly journals Polymerase Chain Reaction and blood culture for diagnosis of canine sepsis

2018 ◽  
Vol 48 (6) ◽  
Author(s):  
Marcelo Marques da Silveira ◽  
Stéfhano Luis Cândido ◽  
Karin Rinaldi dos Santos ◽  
Maerle Oliveira Maia ◽  
Roberto Lopes de Souza ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Blood Culture ◽  
Diagnostic Value ◽  
Turnaround Time ◽  
Diagnostic Techniques ◽  
Pcr Analysis ◽  
Chain Reaction ◽  
Suspected Sepsis ◽  
Fungal Dna ◽  
Polymerase Chain

ABSTRACT: Sepsis is characterized by the presence of organ dysfunction secondary to the dysregulated systemic inflammatory response associated with an infection, and has high mortality rates. Traditional diagnostic techniques based on non-microbiological isolation are time-consuming and may delay treatment. Thus, this study aimed to compare bacterial and fungal broad-range polymerase chain reaction (PCR) and blood culture for diagnosis of sepsis in dogs. Blood samples from 88 dogs with suspected sepsis were analyzed by blood culture, and PCR to detect bacterial and fungal DNA. On blood culture, 20 (22.7%) samples tested positive for bacterial isolates; however, none tested positive for fungi. Through PCR analysis, bacterial DNA was detected in 46 (52.3%) animals, whereas fungal DNA was present in one (1.1%) sample. Our results showed that PCR-based testing has important diagnostic value for canine blood infections because it has a shorter turnaround time and higher sensitivity than traditional blood culture.

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