In search of new anticancer drugs: Data for cytotoxic activities of green synthesized silver nanoparticles from ethanolic extracts of fruits and leaves of Annona muricata and 5-Fluorouracil against HeLa, PC3 and PNT1A cell lines

BACKGROUND: Green synthesized nanoparticles have been earmarked for use in nanomedicine including for the development of better anticancer drugs. OBJECTIVE: The aim of this study was to undertake biochemical evaluation of anticancer activities of green synthesized silver nanoparticles (AgNPs) from ethanolic extracts of fruits (AgNPs-F) and leaves (AgNPs-L) of Annona muricata. METHODS: Previously synthesized silver nanoparticles were used for the study. The effects of the AgNPs and 5-Fluorouracil were studied on PC3, HeLa and PNT1A cells. The resazurin, migration and colonogenic assays as well as qRT-PCR were employed. RESULTS: The AgNPs-F displayed significant antiproliferative effects against HeLa cells with an IC50 of 38.58μg/ml and PC3 cells with an IC50 of 48.17μg/ml but selectively spared normal PNT1A cells (selectivity index of 7.8), in comparison with first line drug 5FU and AgNPs-L whose selectivity index were 3.56 and 2.26 respectively. The migration assay revealed potential inhibition of the metastatic activity of the cells by the AgNPs-F while the colonogenic assay indicated the permanent effect of the AgNPs-F on the cancer cells yet being reversible on the normal cells in contrast with 5FU and AgNPs-L. CASP9 was significantly over expressed in all HeLa cells treated with the AgNPs-F (1.53-fold), AgNPs-L (1.52-fold) and 5FU (4.30-fold). CXCL1 was under expressed in HeLa cells treated with AgNPs-F (0.69-fold) and AgNPs-L (0.58-fold) and over expressed in cells treated with 5FU (4.95-fold), but the difference was not statistically significant. CXCR2 was significantly over expressed in HeLa cells treated with 5FU (8.66-fold) and AgNPs-F (1.12-fold) but under expressed in cells treated with AgNPs-L (0.76-fold). CONCLUSIONS: Here we show that biosynthesized AgNPs especially AgNPs-F can be used in the development of novel and better anticancer drugs. The mechanism of action of the AgNPs involves activation of the intrinsic apoptosis pathway through upregulation of CASP9 and concerted down regulation of the CXCL1/ CXCR2 gene axis.

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Green synthesized silver nanoparticles from Garcinia imberti bourd and their impact on root canal pathogens and HepG2 cell lines

RSC Advances ◽

10.1039/c6ra28328d ◽

2017 ◽

Vol 7 (55) ◽

pp. 34548-34555 ◽

Cited By ~ 7

Author(s):

S. R. Sri Ramkumar ◽

N. Sivakumar ◽

G. Selvakumar ◽

T. Selvankumar ◽

C. Sudhakar ◽

...

Keyword(s):

Silver Nanoparticles ◽

Medicinal Plants ◽

Hepg2 Cell ◽

Cell Lines ◽

Root Canal ◽

Nanoparticle Biosynthesis ◽

Hepg2 Cell Lines ◽

Green Synthesized Silver Nanoparticles

Nanoparticle biosynthesis using the extract of medicinal plants in a non-hazardous mode has gained wide attention for various applications in nanomedicine.

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Photo-assisted bio-fabrication of silver nanoparticles using Annona muricata leaf extract: exploring the antioxidant, anti-diabetic, antimicrobial, and cytotoxic activities

Heliyon ◽

10.1016/j.heliyon.2020.e05413 ◽

2020 ◽

Vol 6 (11) ◽

pp. e05413

Author(s):

J.A. Badmus ◽

S.A. Oyemomi ◽

O.T. Adedosu ◽

T.A. Yekeen ◽

M.A. Azeez ◽

...

Keyword(s):

Silver Nanoparticles ◽

Leaf Extract ◽

Cytotoxic Activities ◽

Annona Muricata

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Evaluation of anti-cholinesterase, antibacterial and cytotoxic activities of green synthesized silver nanoparticles using from Millettia pinnata flower extract

Microbial Pathogenesis ◽

10.1016/j.micpath.2016.12.019 ◽

2017 ◽

Vol 103 ◽

pp. 123-128 ◽

Cited By ~ 35

Author(s):

Govindasamy Rajakumar ◽

Thandapani Gomathi ◽

Muthu Thiruvengadam ◽

V. Devi Rajeswari ◽

V.N. Kalpana ◽

...

Keyword(s):

Silver Nanoparticles ◽

Cytotoxic Activities ◽

Flower Extract ◽

Millettia Pinnata ◽

Green Synthesized Silver Nanoparticles

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Annona muricata assisted biogenic synthesis of silver nanoparticles regulates cell cycle arrest in NSCLC cell lines

Bioorganic Chemistry ◽

10.1016/j.bioorg.2019.103451 ◽

2020 ◽

Vol 95 ◽

pp. 103451 ◽

Cited By ~ 7

Author(s):

Shanmugapriya Meenakshisundaram ◽

Varunkumar Krishnamoorthy ◽

Yogeswaran Jagadeesan ◽

Ravikumar Vilwanathan ◽

Anandaraj Balaiah

Keyword(s):

Cell Cycle ◽

Silver Nanoparticles ◽

Cell Cycle Arrest ◽

Cell Lines ◽

Nsclc Cell ◽

Annona Muricata ◽

Biogenic Synthesis ◽

Cycle Arrest

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Synthesis and Characterization of Silver Nanoparticles from Ethanolic Extracts of Leaves of Annona muricata: A Green Nanobiotechnology Approach

Biotechnology Journal International ◽

10.9734/bji/2019/v23i430083 ◽

2019 ◽

pp. 1-18 ◽

Cited By ~ 1

Author(s):

Yahaya Gavamukulya ◽

Esther N. Maina ◽

Fred Wamunyokoli ◽

Amos M. Meroka ◽

Edwin S. Madivoli ◽

...

Keyword(s):

Electron Microscopy ◽

Silver Nanoparticles ◽

Green Synthesis ◽

Infrared Analysis ◽

Annona Muricata ◽

Medicinal Uses ◽

X Ray ◽

Ethanolic Extracts ◽

Electron Microscopy Analysis ◽

Microscopy Analysis

Introduction: The biological green synthesis of nanoparticles via nanobiotechnology processes have a significant potential to boost nanoparticles production without the use of harsh, toxic, and expensive chemicals commonly used in conventional physical and chemical processes. Annona muricata, a tropical plant belonging to family Annonaceae is one of the most used plants in folk medicine because of its many medicinal uses and therefore presents a strong candidate for use in green synthesis. Aims: The aim of this study was to optimize a method for the synthesis of Silver Nanoparticles (AgNPs) from ethanolic extracts of leaves of Annona muricata as well as to characterize the green synthesized AgNPs. Methodology: AgNPs were synthesized from Annona muricata leaves using AgNO3 solution. The AgNPs were characterized using spectroscopy and microscopy techniques. Results: The formed AgNPs had an absorption maximum at 429 nm using UV–Visible spectroscopy and were stable under different pH, temperature, and storage conditions. Fourier transform infrared analysis revealed the different functional groups responsible for the synthesis and stabilization of the AgNPs. Scanning electron microscopy analysis revealed a spherical nature of the synthesized AgNPs. Energy dispersive x-ray spectroscopy analysis showed presence of Ag, O, and Cl with Ag having the highest composition at 60%. X-Ray Diffraction and Dynamic Light Scattering revealed a crystalline nature of AgNPs with an average size of 87.36 nm and a polydispersity index of 0.16 respectively. Transmission Electron Microscopy analysis further confirmed the crystalline and spherical nature of the AgNPs. Conclusion: In this article, an efficient, eco-friendly and low-cost method for the synthesis and recovery of stable AgNPs using Annona muricata leaves ethanolic extracts as both a reducing and capping agent has been reported for the first time. The synthesized AgNPs could be promising candidates for many biomedical, clinical, engineering, and polymer applications.

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