Involvement of C3435T and G2677T multidrug resistance gene polymorphisms in release of cytokines from peripheral blood mononuclear cells treated with methotrexate and dexamethasone

2005 ◽  
Vol 528 (1-3) ◽  
pp. 27-36 ◽  
Author(s):  
Andrzej Pawlik ◽  
Magdalena Baskiewicz-Masiuk ◽  
Bogusław Machalinski ◽  
Mateusz Kurzawski ◽  
Barbara Gawronska-Szklarz
Keyword(s):  
Multidrug Resistance ◽  
Resistance Gene ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Gene Polymorphisms ◽  
Mononuclear Cells ◽  
Multidrug Resistance Gene ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

scholarly journals Expression of multidrug resistance P-glycoprotein on peripheral blood mononuclear cells of patients with granular lymphocyte-proliferative disorders

Blood ◽  
1993 ◽  
Vol 81 (5) ◽  
pp. 1342-1346
Author(s):  
T Yamamoto ◽  
T Iwasaki ◽  
N Watanabe ◽  
K Oshimi ◽  
M Naito ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Killer Cell ◽  
Chemotherapeutic Agents ◽  
Cell Type ◽  
Peripheral Blood Mononuclear ◽  
P Glycoprotein ◽  
Blood Mononuclear Cells

Some patients with granular lymphocyte-proliferative disorders (GLPD) have been reported to have an aggressive clinical course with a poor prognosis and to be refractory to chemotherapy. In this study, expression of multidrug resistance P-glycoprotein on peripheral blood mononuclear cells (PBMC) of 11 patients with GLPD was examined by staining with MRK 16, a monoclonal antibody that binds to an external epitope of P-glycoprotein, and with the dye rhodamine, a known substance to be excreted from the cells through P-glycoprotein. Among those tested, the PBMC of six of eight patients with T-cell-type GLPD as well as those of three of three patients with natural killer cell- type GLPD expressed P-glycoprotein significantly. Furthermore, PBMC of two of two patients were also poorly stained with the dye rhodamine, and the treatment of PBMC with either verapamil or quinidine, multidrug resistance-reversing agents, led to their increased staining, suggesting that these PBMC actively release chemotherapeutic agents.

scholarly journals Expression of multidrug resistance P-glycoprotein on peripheral blood mononuclear cells of patients with granular lymphocyte-proliferative disorders

Blood ◽  
1993 ◽  
Vol 81 (5) ◽  
pp. 1342-1346 ◽  
Author(s):  
T Yamamoto ◽  
T Iwasaki ◽  
N Watanabe ◽  
K Oshimi ◽  
M Naito ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Killer Cell ◽  
Chemotherapeutic Agents ◽  
Cell Type ◽  
Peripheral Blood Mononuclear ◽  
P Glycoprotein ◽  
Blood Mononuclear Cells

Abstract Some patients with granular lymphocyte-proliferative disorders (GLPD) have been reported to have an aggressive clinical course with a poor prognosis and to be refractory to chemotherapy. In this study, expression of multidrug resistance P-glycoprotein on peripheral blood mononuclear cells (PBMC) of 11 patients with GLPD was examined by staining with MRK 16, a monoclonal antibody that binds to an external epitope of P-glycoprotein, and with the dye rhodamine, a known substance to be excreted from the cells through P-glycoprotein. Among those tested, the PBMC of six of eight patients with T-cell-type GLPD as well as those of three of three patients with natural killer cell- type GLPD expressed P-glycoprotein significantly. Furthermore, PBMC of two of two patients were also poorly stained with the dye rhodamine, and the treatment of PBMC with either verapamil or quinidine, multidrug resistance-reversing agents, led to their increased staining, suggesting that these PBMC actively release chemotherapeutic agents.

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