Micro-computed tomography (micro-CT) for intraoperative surgical margin assessment of breast cancer: A feasibility study in breast conserving surgery

Abstract Background: Surgical margin negativity is highly related to local recurrence of breast cancer. The authors performed this study to evaluate if specimen mammography or ultrasonography can replace the frozen section procedure for surgical margins. Methods: One-hundred fifty five patients with breast cancer were included in this study. After the surgery, the frozen biopsies were assessed in more than three different directions, and all specimens were analyzed with mammography and ultrasonography. The clinicopathologic characteristics of the patients were assessed, and closest tumor margin–resection margin distance (TM–RM distance) to the tumor was compared among specimen mammography, ultrasonography, and pathology. Results: On comparing initial cases of positive and negative margins, the mean closest TM–RM distance in specimen ultrasonography and final pathologic reports was statistically different between both groups (DCIS: p < 0.001, p = 0.006; IDC: p = 0.042, p = 0.022). Conclusion: When the closest TM–RM distance is less than 1.8 mm in specimen ultrasonography, the frozen section cannot be waived because of high risk of margin positivity. However, if the closest TM–RM distance is >4 mm in specimen ultrasonography, the frozen section can be omitted carefully because of the very low risk of margin positivity.

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Intraoperative micro-computed tomography (micro-CT): a novel method for determination of primary tumour dimensions in breast cancer specimens

British Journal of Radiology ◽

10.1259/bjr.20150581 ◽

2016 ◽

Vol 89 (1058) ◽

pp. 20150581 ◽

Cited By ~ 20

Author(s):

Rong Tang ◽

Mansi Saksena ◽

Suzanne B Coopey ◽

Leopoldo Fernandez ◽

Julliette M Buckley ◽

...

Keyword(s):

Breast Cancer ◽

Computed Tomography ◽

Primary Tumour ◽

Micro Ct ◽

Micro Computed Tomography ◽

Novel Method

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Micro-computed tomography (Micro-CT): a novel approach for intraoperative breast cancer specimen imaging

Breast Cancer Research and Treatment ◽

10.1007/s10549-013-2554-6 ◽

2013 ◽

Vol 139 (2) ◽

pp. 311-316 ◽

Cited By ~ 27

Author(s):

Rong Tang ◽

Julliette M. Buckley ◽

Leopoldo Fernandez ◽

Suzanne Coopey ◽

Owen Aftreth ◽

...

Keyword(s):

Breast Cancer ◽

Computed Tomography ◽

Micro Ct ◽

Micro Computed Tomography ◽

Breast Cancer Specimen ◽

Novel Approach ◽

Cancer Specimen

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Feasibility of Perioperative Micro–Computed Tomography of Human Lung Cancer Specimens: A Pilot Study

Archives of Pathology & Laboratory Medicine ◽

10.5858/arpa.2018-0249-oa ◽

2018 ◽

Vol 143 (3) ◽

pp. 319-325 ◽

Cited By ~ 2

Author(s):

Fabian M. Troschel ◽

Ravi V. Gottumukkala ◽

Daniel DiCorpo ◽

Julia Mario ◽

Harald C. Ott ◽

...

Keyword(s):

Computed Tomography ◽

Lung Cancer ◽

Lung Resection ◽

Surgical Margin ◽

Lesion Size ◽

Ct Images ◽

Micro Ct ◽

Micro Computed Tomography ◽

Limits Of Agreement ◽

Lesion Localization

Context.— Lesion localization during intraoperative frozen section of lung resection specimens can be challenging. Imaging could aid lesion localization while enabling 3-dimensional specimen analysis. Objective.— To assess the feasibility of integrating micro–computed tomography (micro-CT) into the perioperative evaluation of fresh surgical lung resection specimens. Design.— Fresh lung specimens from patients with a presumptive diagnosis of lung cancer were imaged with micro-CT prior to routine histopathologic and molecular analysis. Micro-CT images were assessed to determine image quality, lesion size, and distance from lesion to the nearest surgical margin. Micro-CT measurements were compared to pathologic measurements using Bland-Altman analysis. Results.— A total of 22 specimens from 21 patients were analyzed (mean image acquisition time, 13 ± 6 minutes). Histologic quality of imaged specimens was indistinguishable from a control group of nonimaged lung specimens. Artifacts, most commonly from specimen deflation (n = 8), obscured fine detail on micro-CT images of 10 specimens. Micro-CT could successfully localize the target lesion in the other 12 specimens. Distance to the nearest surgical margin was determined in 10 specimens. Agreement of micro-CT with final pathology was good, with a mean difference of −2.8% (limits of agreement −14.5% to 20.0%) for lesion size and −0.5 mm (limits of agreement −4.4 to 3.4 mm) for distance to nearest surgical margin. Conclusions.— Micro-CT of fresh surgical lung specimens is feasible and has the potential to evaluate the size and location of lesions within resection specimens, as well as distance to the nearest surgical margin, all without compromising specimen integrity.

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Micro-Computed Tomography Analysis of Subchondral Bone Regeneration Using Osteochondral Scaffolds in an Ovine Condyle Model

Applied Sciences ◽

10.3390/app11030891 ◽

2021 ◽

Vol 11 (3) ◽

pp. 891

Author(s):

Taylor Flaherty ◽

Maryam Tamaddon ◽

Chaozong Liu

Keyword(s):

Computed Tomography ◽

Bone Regeneration ◽

Subchondral Bone ◽

Volume Ratio ◽

Bone Volume ◽

Osteochondral Defects ◽

Micro Ct ◽

Micro Computed Tomography ◽

Trabecular Thickness ◽

Osteochondral Scaffold

Osteochondral scaffold technology has emerged as a promising therapy for repairing osteochondral defects. Recent research suggests that seeding osteochondral scaffolds with bone marrow concentrate (BMC) may enhance tissue regeneration. To examine this hypothesis, this study examined subchondral bone regeneration in scaffolds with and without BMC. Ovine stifle condyle models were used for the in vivo study. Two scaffold systems (8 mm diameter and 10 mm thick) with and without BMC were implanted into the femoral condyle, and the tissues were retrieved after six months. The retrieved femoral condyles (with scaffold in) were examined using micro-computed tomography scans (micro-CT), and the micro-CT data were further analysed by ImageJ with respect to trabecular thickness, bone volume to total volume ratio (BV/TV) ratio, and degree of anisotropy of bone. Statistical analysis compared bone regeneration between scaffold groups and sub-set regions. These results were mostly insignificant (p < 0.05), with the exception of bone volume to total volume ratio when comparing scaffold composition and sub-set region. Additional trends in the data were observed. These results suggest that the scaffold composition and addition of BMC did not significantly affect bone regeneration in osteochondral defects after six months. However, this research provides data which may guide the development of future treatments.

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Using micro-computed tomography to reveal the anatomy of adult Diaphorina citri Kuwayama (Insecta: Hemiptera, Liviidae) and how it pierces and feeds within a citrus leaf

Scientific Reports ◽

10.1038/s41598-020-80404-z ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Javier Alba-Tercedor ◽

Wayne B. Hunter ◽

Ignacio Alba-Alejandre

Keyword(s):

Computed Tomography ◽

Significant Advance ◽

Micro Ct ◽

Diaphorina Citri ◽

Ct Reconstruction ◽

Internal Anatomy ◽

Micro Computed Tomography ◽

Citrus Leaf ◽

Teaching Aids ◽

Adult Feeding

AbstractThe Asian citrus psyllid (ACP), Diaphorina citri, is a harmful pest of citrus trees that transmits Candidatus Liberibacter spp. which causes Huanglongbing (HLB) (citrus greening disease); this is considered to be the most serious bacterial disease of citrus plants. Here we detail an anatomical study of the external and internal anatomy (excluding the reproductive system) using micro-computed tomography (micro-CT). This is the first complete 3D micro-CT reconstruction of the anatomy of a psylloid insect and includes a 3D reconstruction of an adult feeding on a citrus leaf that can be used on mobile devices. Detailed rendered images and videos support first descriptions of coxal and scapus antennal glands and sexual differences in the internal anatomy (hindgut rectum, mesothoracic ganglion and brain). This represents a significant advance in our knowledge of ACP anatomy, and of psyllids in general. Together the images, videos and 3D model constitute a unique anatomical atlas and are useful tools for future research and as teaching aids.

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MicroCT protocols for scanning of embryos and juvenile Hexaplex trunculus v1

10.17504/protocols.io.bxw4ppgw ◽

2021 ◽

Author(s):

Eva Chatzinikolaou ◽

Kleoniki Keklikoglou

Keyword(s):

Computed Tomography ◽

High Resolution ◽

3D Imaging ◽

Imaging Technique ◽

Ct Scanning ◽

Micro Ct ◽

Micro Computed Tomography ◽

Gastropod Species ◽

Biological Studies ◽

Hexaplex Trunculus

Micro-computed tomography (micro-CT) is a high-resolution 3D-imaging technique which is now increasingly applied in biological studies focusing on taxonomy and functional morphology. The creation of virtual representations of specimens can increase availability of otherwise underexploited and inaccessible samples. This protocol aims to standardise micro-CT scanning procedures for embryos and juveniles of the marine gastropod species Hexaplex trunculus.

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