scholarly journals Micro-Computed Tomography Analysis of Subchondral Bone Regeneration Using Osteochondral Scaffolds in an Ovine Condyle Model

2021 ◽  
Vol 11 (3) ◽  
pp. 891
Author(s):  
Taylor Flaherty ◽  
Maryam Tamaddon ◽  
Chaozong Liu
Keyword(s):  
Computed Tomography ◽  
Bone Regeneration ◽  
Subchondral Bone ◽  
Volume Ratio ◽  
Bone Volume ◽  
Osteochondral Defects ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Trabecular Thickness ◽  
Osteochondral Scaffold

Osteochondral scaffold technology has emerged as a promising therapy for repairing osteochondral defects. Recent research suggests that seeding osteochondral scaffolds with bone marrow concentrate (BMC) may enhance tissue regeneration. To examine this hypothesis, this study examined subchondral bone regeneration in scaffolds with and without BMC. Ovine stifle condyle models were used for the in vivo study. Two scaffold systems (8 mm diameter and 10 mm thick) with and without BMC were implanted into the femoral condyle, and the tissues were retrieved after six months. The retrieved femoral condyles (with scaffold in) were examined using micro-computed tomography scans (micro-CT), and the micro-CT data were further analysed by ImageJ with respect to trabecular thickness, bone volume to total volume ratio (BV/TV) ratio, and degree of anisotropy of bone. Statistical analysis compared bone regeneration between scaffold groups and sub-set regions. These results were mostly insignificant (p < 0.05), with the exception of bone volume to total volume ratio when comparing scaffold composition and sub-set region. Additional trends in the data were observed. These results suggest that the scaffold composition and addition of BMC did not significantly affect bone regeneration in osteochondral defects after six months. However, this research provides data which may guide the development of future treatments.

3D-printed Poly-Lactic Co-Glycolic Acid (PLGA) scaffolds in non-critical bone defects impede bone regeneration in rabbit tibia bone

2021 ◽  
pp. 1-7
Author(s):  
Jin Xi Lim ◽  
Min He ◽  
Alphonsus Khin Sze Chong
Keyword(s):  
Computed Tomography ◽  
Bone Regeneration ◽  
Bone Defect ◽  
Volume Ratio ◽  
Bone Defects ◽  
Control Group ◽  
Micro Computed Tomography ◽  
Rabbit Tibia ◽  
3D Printed ◽  
Critical Bone Defects

BACKGROUND: An increasing number of bone graft materials are commercially available and vary in their composition, mechanism of action, costs, and indications. OBJECTIVE: A commercially available PLGA scaffold produced using 3D printing technology has been used to promote the preservation of the alveolar socket after tooth extraction. We examined its influence on bone regeneration in long bones of New Zealand White rabbits. METHODS: 5.0-mm-diameter circular defects were created on the tibia bones of eight rabbits. Two groups were studied: (1) control group, in which the bone defects were left empty; (2) scaffold group, in which the PLGA scaffolds were implanted into the bone defect. Radiography was performed every two weeks postoperatively. After sacrifice, bone specimens were isolated and examined by micro-computed tomography and histology. RESULTS: Scaffolds were not degraded by eight weeks after surgery. Micro-computed tomography and histology showed that in the region of bone defects that was occupied by scaffolds, bone regeneration was compromised and the total bone volume/total volume ratio (BV/TV) was significantly lower. CONCLUSION: The implantation of this scaffold impedes bone regeneration in a non-critical bone defect. Implantation of bone scaffolds, if unnecessary, lead to a slower rate of fracture healing.

scholarly journals Spatio-temporal characterization of fracture healing patterns and assessment of biomaterials by time-lapsed in vivo micro-computed tomography

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Esther Wehrle ◽  
Duncan C. Tourolle né Betts ◽  
Gisela A. Kuhn ◽  
Erica Floreani ◽  
Malavika H. Nambiar ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Bone Regeneration ◽  
Healing Process ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Collagen Scaffolds ◽  
Non Union ◽  
Spatio Temporal

AbstractThorough preclinical evaluation of functionalized biomaterials for treatment of large bone defects is essential prior to clinical application. Using in vivo micro-computed tomography (micro-CT) and mouse femoral defect models with different defect sizes, we were able to detect spatio-temporal healing patterns indicative of physiological and impaired healing in three defect sub-volumes and the adjacent cortex. The time-lapsed in vivo micro-CT-based approach was then applied to evaluate the bone regeneration potential of functionalized biomaterials using collagen and bone morphogenetic protein (BMP-2). Both collagen and BMP-2 treatment led to distinct changes in bone turnover in the different healing phases. Despite increased periosteal bone formation, 87.5% of the defects treated with collagen scaffolds resulted in non-unions. Additional BMP-2 application significantly accelerated the healing process and increased the union rate to 100%. This study further shows potential of time-lapsed in vivo micro-CT for capturing spatio-temporal deviations preceding non-union formation and how this can be prevented by application of functionalized biomaterials. This study therefore supports the application of longitudinal in vivo micro-CT for discrimination of normal and disturbed healing patterns and for the spatio-temporal characterization of the bone regeneration capacity of functionalized biomaterials.

scholarly journals Using micro-computed tomography to reveal the anatomy of adult Diaphorina citri Kuwayama (Insecta: Hemiptera, Liviidae) and how it pierces and feeds within a citrus leaf

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Javier Alba-Tercedor ◽  
Wayne B. Hunter ◽  
Ignacio Alba-Alejandre
Keyword(s):  
Computed Tomography ◽  
Significant Advance ◽  
Micro Ct ◽  
Diaphorina Citri ◽  
Ct Reconstruction ◽  
Internal Anatomy ◽  
Micro Computed Tomography ◽  
Citrus Leaf ◽  
Teaching Aids ◽  
Adult Feeding

AbstractThe Asian citrus psyllid (ACP), Diaphorina citri, is a harmful pest of citrus trees that transmits Candidatus Liberibacter spp. which causes Huanglongbing (HLB) (citrus greening disease); this is considered to be the most serious bacterial disease of citrus plants. Here we detail an anatomical study of the external and internal anatomy (excluding the reproductive system) using micro-computed tomography (micro-CT). This is the first complete 3D micro-CT reconstruction of the anatomy of a psylloid insect and includes a 3D reconstruction of an adult feeding on a citrus leaf that can be used on mobile devices. Detailed rendered images and videos support first descriptions of coxal and scapus antennal glands and sexual differences in the internal anatomy (hindgut rectum, mesothoracic ganglion and brain). This represents a significant advance in our knowledge of ACP anatomy, and of psyllids in general. Together the images, videos and 3D model constitute a unique anatomical atlas and are useful tools for future research and as teaching aids.

scholarly journals Osseointegration of a novel dental implant in canine

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Lingxiao Wang ◽  
Zhenhua Gao ◽  
Yucheng Su ◽  
Qian Liu ◽  
Yi Ge ◽  
...  
Keyword(s):  
Volume Ratio ◽  
Volume Density ◽  
Scientific Basis ◽  
Bone Volume ◽  
Trabecular Thickness ◽  
Bone Implant ◽  
Further Development ◽  
Trabecular Number ◽  
Histological Staining

AbstractThis study aimed to compare and verify the osseointegration performance of a novel implant (NI) in vivo, which could provide a useful scientific basis for the further development of NIs. Thirty-two NIs treated with hydrofluoric acid and anodization and sixteen control implants (CIs) were placed in the mandibles of 8 beagles. Micro-CT showed that the trabecular number (Tb.N) significantly increased and trabecular separation (Tb.Sp) significantly decreased in the NIs at 2 weeks. Significant differences were found in the trabecular thickness, Tb.N, Tb.Sp, bone surface/bone volume ratio, and bone volume/total volume ratio between the two groups from the 2nd–4th weeks. However, there were no significant differences between the two groups in the bone volume density at 2, 4, 8, or 12 weeks or bone-implant contact at 2 or 4 weeks, but the BIC in the CIs was higher than that in the NIs at the 8th and 12th weeks. Meanwhile, the histological staining showed a similar osseointegration process between the two groups over time. Overall, the NIs could be used as new potential implants after further improvement.

scholarly journals Longitudinal in vivo evaluation of bone regeneration by combined measurement of multi-pinhole SPECT and micro-CT for tissue engineering

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Philipp S. Lienemann ◽  
Stéphanie Metzger ◽  
Anna-Sofia Kiveliö ◽  
Alain Blanc ◽  
Panagiota Papageorgiou ◽  
...  
Keyword(s):  
Computed Tomography ◽  
High Resolution ◽  
Bone Formation ◽  
Bone Regeneration ◽  
Bone Defects ◽  
Histological Evaluation ◽  
Biological Processes ◽  
Micro Ct ◽  
Pinhole Spect

Abstract Over the last decades, great strides were made in the development of novel implants for the treatment of bone defects. The increasing versatility and complexity of these implant designs request for concurrent advances in means to assess in vivo the course of induced bone formation in preclinical models. Since its discovery, micro-computed tomography (micro-CT) has excelled as powerful high-resolution technique for non-invasive assessment of newly formed bone tissue. However, micro-CT fails to provide spatiotemporal information on biological processes ongoing during bone regeneration. Conversely, due to the versatile applicability and cost-effectiveness, single photon emission computed tomography (SPECT) would be an ideal technique for assessing such biological processes with high sensitivity and for nuclear imaging comparably high resolution (<1 mm). Herein, we employ modular designed poly(ethylene glycol)-based hydrogels that release bone morphogenetic protein to guide the healing of critical sized calvarial bone defects. By combined in vivo longitudinal multi-pinhole SPECT and micro-CT evaluations we determine the spatiotemporal course of bone formation and remodeling within this synthetic hydrogel implant. End point evaluations by high resolution micro-CT and histological evaluation confirm the value of this approach to follow and optimize bone-inducing biomaterials.

Non-Invasive Analysis of the Micro-Structural and Biomechanical Properties of Trabecular Bone in Human Femoral Head

2006 ◽  
Vol 321-323 ◽  
pp. 1070-1073
Author(s):  
Ye Yeon Won ◽  
Myong Hyun Baek ◽  
Wen Quan Cui ◽  
Kwang Kyun Kim
Keyword(s):  
Mechanical Properties ◽  
Mechanical Property ◽  
Femoral Head ◽  
Trabecular Bone ◽  
Volume Fraction ◽  
Reaction Force ◽  
Bone Volume ◽  
Fe Model ◽  
Micro Ct ◽  
Trabecular Thickness

This study investigates micro-structural and mechanical properties of trabecular bone in human femoral head with and without osteoporosis using a micro-CT and a finite element model. 15 cored trabecular bone specimens with 20 of diameter were obtained from femoral heads with osteoporosis resected for total hip arthroplasty, and 5 specimens were removed from femoral head of cadavers, which has no history of musculoskeletal diseases. A high-resolution micro-CT system was used to scan each specimen to obtain histomorphometry indexes. Based on the micro-images, a FE-model was created to determine mechanical property indexes. While the non-osteoporosis group had increases the trabecular thickness, the bone volume, the bone volume fraction, the degree of anisotropy and the trabecular number compared with those of osteoporotic group, the non-osteoporotic group showed decreases in trabecular separation and structure model index. Regarding the mechanical property indexes, the reaction force and the Young's modulus were lower in the osteoporotic group than in non-osteoporotic group. Our data shows salient deteriorations in trabecular micro-structural and mechanical properties in human femoral head with osteoporosis.

MicroCT protocols for scanning of embryos and juvenile Hexaplex trunculus v1

2021 ◽  
Author(s):  
Eva Chatzinikolaou ◽  
Kleoniki Keklikoglou
Keyword(s):  
Computed Tomography ◽  
High Resolution ◽  
3D Imaging ◽  
Imaging Technique ◽  
Ct Scanning ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Gastropod Species ◽  
Biological Studies ◽  
Hexaplex Trunculus

Micro-computed tomography (micro-CT) is a high-resolution 3D-imaging technique which is now increasingly applied in biological studies focusing on taxonomy and functional morphology. The creation of virtual representations of specimens can increase availability of otherwise underexploited and inaccessible samples. This protocol aims to standardise micro-CT scanning procedures for embryos and juveniles of the marine gastropod species Hexaplex trunculus.

scholarly journals Micro-Computed Tomography (Micro-CT) Evaluation of Effects of Different Rotary Glide Path Techniques on Canal Transportation and Centering in Curved Root Canals

2019 ◽  
Vol 25 ◽  
pp. 6351-6358
Author(s):  
Gabrielė Česaitienė ◽  
Tadas Venskutonis ◽  
Vita Mačiulskienė ◽  
Vaidotas Cicėnas ◽  
Vykintas Samaitis ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Root Canals ◽  
Glide Path ◽  
Ct Evaluation ◽  
Canal Transportation ◽  
Curved Root Canals

Quantitative micro-computed tomography analysis and rheological investigation of Nitrile rubber/Rockwool composites

2021 ◽  
pp. 002199832110338
Author(s):  
Elisson BD da Rocha ◽  
Ana Maria F de Sousa ◽  
Ana Lúcia N da Silva ◽  
Cristina RG Furtado ◽  
Marcos V Colaço ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Elastic Modulus ◽  
Aspect Ratio ◽  
Volume Fraction ◽  
Nitrile Rubber ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Rubber Composites ◽  
Ratio Data ◽  
Ct Analysis

This study reports the reinforcement degree investigation of two types of rockwool fibers (F1 and F2), in nitrile rubber composites. The micro-computed tomography (micro-CT) 3D images showed that both fibers were well-dispersed in the NBR matrix, without a preferential orientation. The micro-CT analysis also allowed quantifying volume fraction, inter-fiber distance, and aspect ratio. Those morphometric parameters were used for supporting the composites rheological behavior assessment. Changes in the elastic modulus and phase angle followed the same trend of the inter-fiber distance values, regardless the type of fiber. Both volume fraction and aspect ratio data from the micro-CT analysis were used to predict theoretical values of elastic modulus using the Guth-Gold and modified Guth-Gold equations, and the results obtained were compared to the rheological experimental data. This analysis was helpful to better understand the rockwool fibers reinforcement degree differences in the production of the nitrile rubber composites.

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