scholarly journals Using micro-computed tomography to reveal the anatomy of adult Diaphorina citri Kuwayama (Insecta: Hemiptera, Liviidae) and how it pierces and feeds within a citrus leaf

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Javier Alba-Tercedor ◽  
Wayne B. Hunter ◽  
Ignacio Alba-Alejandre
Keyword(s):  
Computed Tomography ◽  
Significant Advance ◽  
Micro Ct ◽  
Diaphorina Citri ◽  
Ct Reconstruction ◽  
Internal Anatomy ◽  
Micro Computed Tomography ◽  
Citrus Leaf ◽  
Teaching Aids ◽  
Adult Feeding

AbstractThe Asian citrus psyllid (ACP), Diaphorina citri, is a harmful pest of citrus trees that transmits Candidatus Liberibacter spp. which causes Huanglongbing (HLB) (citrus greening disease); this is considered to be the most serious bacterial disease of citrus plants. Here we detail an anatomical study of the external and internal anatomy (excluding the reproductive system) using micro-computed tomography (micro-CT). This is the first complete 3D micro-CT reconstruction of the anatomy of a psylloid insect and includes a 3D reconstruction of an adult feeding on a citrus leaf that can be used on mobile devices. Detailed rendered images and videos support first descriptions of coxal and scapus antennal glands and sexual differences in the internal anatomy (hindgut rectum, mesothoracic ganglion and brain). This represents a significant advance in our knowledge of ACP anatomy, and of psyllids in general. Together the images, videos and 3D model constitute a unique anatomical atlas and are useful tools for future research and as teaching aids.

scholarly journals Anatomical study of the coffee berry borer (Hypothenemus hampei) using micro-computed tomography

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Ignacio Alba-Alejandre ◽  
Javier Alba-Tercedor ◽  
Fernando E. Vega
Keyword(s):  
Computed Tomography ◽  
Future Research ◽  
Micro Ct ◽  
Ct Reconstruction ◽  
Hypothenemus Hampei ◽  
Internal Anatomy ◽  
Micro Computed Tomography ◽  
Coffee Berry ◽  
Coffee Berry Borer ◽  
Teaching Aids

AbstractTraditionally, the study of anatomy in insects has been based on dissection techniques. Micro-computed tomography (micro-CT) is an X-ray based technique that allows visualization of the internal anatomy of insects in situ and does not require dissections. We report on the use of micro-CT scans to study, in detail, the internal structures and organs of the coffee berry borer (Hypothenemus hampei), the most damaging insect pest of coffee worldwide. Detailed images and videos allowed us to make the first description of the aedeagus and the first report of differences between the sexes based on internal anatomy (flight musculature, midgut shape, hindgut convolutions, brain shape and size) and external morphology (lateral outline of the pronotum and number of abdominal tergites). This study is the first complete micro-CT reconstruction of the anatomy of an insect and is also the smallest insect to have been evaluated in this way. High quality rendered images, and additional supplementary videos and 3D models are suitable for use with mobile devices and are useful tools for future research and as teaching aids.

scholarly journals Publisher Correction: Using micro-computed tomography to reveal the anatomy of adult Diaphorina citri Kuwayama (Insecta: Hemiptera, Liviidae) and how it pierces and feeds within a citrus leaf

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Javier Alba‑Tercedor ◽  
Wayne B. Hunter ◽  
Ignacio Alba‑Alejandre
Keyword(s):  
Computed Tomography ◽  
Diaphorina Citri ◽  
Micro Computed Tomography ◽  
Citrus Leaf

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

scholarly journals Micro-Computed Tomography Analysis of Subchondral Bone Regeneration Using Osteochondral Scaffolds in an Ovine Condyle Model

2021 ◽  
Vol 11 (3) ◽  
pp. 891
Author(s):  
Taylor Flaherty ◽  
Maryam Tamaddon ◽  
Chaozong Liu
Keyword(s):  
Computed Tomography ◽  
Bone Regeneration ◽  
Subchondral Bone ◽  
Volume Ratio ◽  
Bone Volume ◽  
Osteochondral Defects ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Trabecular Thickness ◽  
Osteochondral Scaffold

Osteochondral scaffold technology has emerged as a promising therapy for repairing osteochondral defects. Recent research suggests that seeding osteochondral scaffolds with bone marrow concentrate (BMC) may enhance tissue regeneration. To examine this hypothesis, this study examined subchondral bone regeneration in scaffolds with and without BMC. Ovine stifle condyle models were used for the in vivo study. Two scaffold systems (8 mm diameter and 10 mm thick) with and without BMC were implanted into the femoral condyle, and the tissues were retrieved after six months. The retrieved femoral condyles (with scaffold in) were examined using micro-computed tomography scans (micro-CT), and the micro-CT data were further analysed by ImageJ with respect to trabecular thickness, bone volume to total volume ratio (BV/TV) ratio, and degree of anisotropy of bone. Statistical analysis compared bone regeneration between scaffold groups and sub-set regions. These results were mostly insignificant (p < 0.05), with the exception of bone volume to total volume ratio when comparing scaffold composition and sub-set region. Additional trends in the data were observed. These results suggest that the scaffold composition and addition of BMC did not significantly affect bone regeneration in osteochondral defects after six months. However, this research provides data which may guide the development of future treatments.

MicroCT protocols for scanning of embryos and juvenile Hexaplex trunculus v1

2021 ◽  
Author(s):  
Eva Chatzinikolaou ◽  
Kleoniki Keklikoglou
Keyword(s):  
Computed Tomography ◽  
High Resolution ◽  
3D Imaging ◽  
Imaging Technique ◽  
Ct Scanning ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Gastropod Species ◽  
Biological Studies ◽  
Hexaplex Trunculus

Micro-computed tomography (micro-CT) is a high-resolution 3D-imaging technique which is now increasingly applied in biological studies focusing on taxonomy and functional morphology. The creation of virtual representations of specimens can increase availability of otherwise underexploited and inaccessible samples. This protocol aims to standardise micro-CT scanning procedures for embryos and juveniles of the marine gastropod species Hexaplex trunculus.

scholarly journals Micro-Computed Tomography (Micro-CT) Evaluation of Effects of Different Rotary Glide Path Techniques on Canal Transportation and Centering in Curved Root Canals

2019 ◽  
Vol 25 ◽  
pp. 6351-6358
Author(s):  
Gabrielė Česaitienė ◽  
Tadas Venskutonis ◽  
Vita Mačiulskienė ◽  
Vaidotas Cicėnas ◽  
Vykintas Samaitis ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Root Canals ◽  
Glide Path ◽  
Ct Evaluation ◽  
Canal Transportation ◽  
Curved Root Canals

Quantitative micro-computed tomography analysis and rheological investigation of Nitrile rubber/Rockwool composites

2021 ◽  
pp. 002199832110338
Author(s):  
Elisson BD da Rocha ◽  
Ana Maria F de Sousa ◽  
Ana Lúcia N da Silva ◽  
Cristina RG Furtado ◽  
Marcos V Colaço ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Elastic Modulus ◽  
Aspect Ratio ◽  
Volume Fraction ◽  
Nitrile Rubber ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Rubber Composites ◽  
Ratio Data ◽  
Ct Analysis

This study reports the reinforcement degree investigation of two types of rockwool fibers (F1 and F2), in nitrile rubber composites. The micro-computed tomography (micro-CT) 3D images showed that both fibers were well-dispersed in the NBR matrix, without a preferential orientation. The micro-CT analysis also allowed quantifying volume fraction, inter-fiber distance, and aspect ratio. Those morphometric parameters were used for supporting the composites rheological behavior assessment. Changes in the elastic modulus and phase angle followed the same trend of the inter-fiber distance values, regardless the type of fiber. Both volume fraction and aspect ratio data from the micro-CT analysis were used to predict theoretical values of elastic modulus using the Guth-Gold and modified Guth-Gold equations, and the results obtained were compared to the rheological experimental data. This analysis was helpful to better understand the rockwool fibers reinforcement degree differences in the production of the nitrile rubber composites.

scholarly journals Effect of micro-computed tomography reconstruction protocols on bone fractal dimension analysis

2019 ◽  
Vol 48 (8) ◽  
pp. 20190235
Author(s):  
Hugo Gaêta-Araujo ◽  
Nicolly Oliveira-Santos ◽  
Danieli Moura Brasil ◽  
Eduarda Helena Leandro do Nascimento ◽  
Daniela Verardi Madlum ◽  
...  
Keyword(s):  
Fractal Dimension ◽  
Trabecular Bone ◽  
Micro Ct ◽  
Ct Reconstruction ◽  
Micro Computed Tomography ◽  
Beam Hardening ◽  
Scan Time ◽  
Beam Hardening Correction ◽  
Box Counting Method ◽  
Artefact Correction

Objectives: To evaluate the influence of the level of three micro-CT reconstruction tools: beam-hardening correction (BHC), smoothing filter (SF), and ring artefact correction (RAC) on the fractal dimension (FD) analysis of trabecular bone. Methods: Five Wistar rats’ maxillae were individually scanned in a SkyScan 1174 micro-CT device, under the following settings: 50 kV, 800 µA, 10.2 µm voxel size, 0.5 mm Al filter, rotation step 0.5°, two frames average, 180° rotation and scan time of 35 min. The raw images were reconstructed under the standard protocol (SP) recommended by the manufacturer, a protocol without any artefact correction tools (P0) and 35 additional protocols with different combinations of SF, RAC and BHC levels. The same volume of interest was established in all reconstructions for each maxilla and the FD was calculated using the Kolmogorov (box counting) method. One-way ANOVA with Dunnet’s post-hoc test was used to compare the FD of each reconstruction protocol (P0–P35) with the SP (α = 5%). Multiple linear regression verified the dependency of reconstruction tools in FD. Results: Overall, FD values are not dependent on RAC (p = 0.965), but increased significantly when the level of BHC and SF increased (p < 0.001). FD values from protocols with BHC at 45% combined with SF of 2, and BHC at 30% combined with SF of 4 or 6 had no statistical difference compared to SP. Conclusions: BHC and SF tools affect the FD values of micro-CT images of the trabecular bone. Therefore, these reconstruction parameters should be standardized when the FD is analyzed.

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