Characterization of Rheum ribes with ZnO nanoparticle and its antidiabetic, antibacterial, DNA damage prevention and lipid peroxidation prevention activity of in vitro

2021 ◽  
pp. 112363
Author(s):  
Ismet Meydan ◽  
Hakan Burhan ◽  
Tuğba Gür ◽  
Hamdullah Seçkin ◽  
Bahareh Tanhaei ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Dna Damage ◽  
Zno Nanoparticle ◽  
Prevention Activity ◽  
Damage Prevention

scholarly journals In Vitro Biological Characterization of DCUN1D5 in DNA Damage Response

2012 ◽  
Vol 13 (8) ◽  
pp. 4157-4162 ◽  
Author(s):  
Wei Guo ◽  
Guo-Jun Li ◽  
Hong-Bo Xu ◽  
Jie-Shi Xie ◽  
Tai-Ping Shi ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Damage Response ◽  
Biological Characterization ◽  
Damage Response

403. Differential effect of hexoses on sperm metabolism and function in culture

2008 ◽  
Vol 20 (9) ◽  
pp. 83
Author(s):  
H. W. Bakos ◽  
M. Lane
Keyword(s):  
Lipid Peroxidation ◽  
Dna Damage ◽  
Carbohydrate Metabolism ◽  
Culture Media ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Sperm Function ◽  
Ros Production ◽  
Sperm Dna Damage ◽  
The Media

Currently there is lack information regarding how human spermatozoa regulate their energy metabolism. This is surprising considering that carbohydrate metabolism is a vital point for the understanding of sperm function. This coupled with the increased use of assisted reproductive technology and the importance of a well balanced culture media has led us to hypothesise that an imbalance of carbohydrate presence in the media may alter sperm function, particularly in relation to oxidative stress, DNA damage and lipid peroxidation. Sperm samples were obtained from three healthy normospermic donors for this study. Motile sperm were separated from semen samples using density gradient separation. Samples were incubated at different media conditions with varying glucose or fructose concentrations (0, 2.5, 25 mM) for 6–24hrs. Reactive oxygen species (ROS) were measured using 5-(and-6)-carboxy-2', 7'-dichlorofluorescein diacetate (DCFDA). Sperm DNA damage was determined using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-nick end labelling (TUNEL). Lipid peroxidation was assessed using the probe BODIPY (581/591) C11. Carbohydrate uptake from the media was measured using a fluorometric procedure. Statistical differences between treatments were assessed by ANOVA and Bonferroni post-hoc test. No significant motility differences were found following treatments. Results showed an increased level of ROS production as glucose concentration increased (P < 0.05). This was accompanied by an increased number of TUNEL positive cells (P < 0.05). Furthermore, lipid peroxidation of spermatozoa was significantly increased when incubated under high glucose concentrations (P < 0.01). In contrast, increases in fructose concentrations did not alter ROS levels or the number of TUNEL positive cells. Sperm metabolised both glucose and fructose in vitro and the removal of one carbohydrate resulted in a compensatory increase in the metabolism of the other. To our knowledge, this is the first report providing evidence that altered carbohydrate metabolism may induce ROS production, lipid peroxidation and increase the number of sperm exhibiting DNA damage.

scholarly journals In vivo and in vitro characterization of DdrC, a DNA damage response protein in Deinococcus radiodurans bacterium

PLoS ONE ◽  
2017 ◽  
Vol 12 (5) ◽  
pp. e0177751 ◽  
Author(s):  
Claire Bouthier de la Tour ◽  
Martine Mathieu ◽  
Laura Meyer ◽  
Pauline Dupaigne ◽  
Fanny Passot ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Damage Response ◽  
Deinococcus Radiodurans ◽  
In Vitro Characterization ◽  
Damage Response

scholarly journals Nucleophosmin Is Required for DNA Integrity and p19Arf Protein Stability

2005 ◽  
Vol 25 (20) ◽  
pp. 8874-8886 ◽  
Author(s):  
Emanuela Colombo ◽  
Paola Bonetti ◽  
Eros Lazzerini Denchi ◽  
Paola Martinelli ◽  
Raffaella Zamponi ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Dna Damage ◽  
Genetic Alterations ◽  
Dna Integrity ◽  
Senescent Phenotype ◽  
Tumor Suppressive Function ◽  
Proliferation In Vitro

ABSTRACT Nucleophosmin (NPM) is a nucleolar phosphoprotein that binds the tumor suppressors p53 and p19Arf and is thought to be indispensable for ribogenesis, cell proliferation, and survival after DNA damage. The NPM gene is the most frequent target of genetic alterations in leukemias and lymphomas, though its role in tumorigenesis is unknown. We report here the first characterization of a mouse NPM knockout strain. Lack of NPM expression results in accumulation of DNA damage, activation of p53, widespread apoptosis, and mid-stage embryonic lethality. Fibroblasts explanted from null embryos fail to grow and rapidly acquire a senescent phenotype. Transfer of the NPM mutation into a p53-null background rescued apoptosis in vivo and fibroblast proliferation in vitro. Cells null for both p53 and NPM grow faster than control cells and are more susceptible to transformation by activated oncogenes, such as mutated Ras or overexpressed Myc. In the absence of NPM, Arf protein is excluded from nucleoli and is markedly less stable. Our data demonstrate that NPM regulates DNA integrity and, through Arf, inhibits cell proliferation and are consistent with a putative tumor-suppressive function of NPM.

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