scholarly journals Development of an autotrophic culture system for the in vitro mycorrhization of potato plantlets

2005 ◽  
Vol 248 (1) ◽  
pp. 111-118 ◽  
Author(s):  
Liesbeth Voets ◽  
Hervé Dupré de Boulois ◽  
Laurent Renard ◽  
Désiré-Georges Strullu ◽  
Stéphane Declerck
Keyword(s):  
Culture System ◽  
Autotrophic Culture ◽  
Potato Plantlets

scholarly journals Evaluación de un sistema para la micorrización in vitro en plantas de mora de castilla (Rubus glaucus, Benth)

2012 ◽  
Vol 17 (2) ◽  
pp. 140 ◽  
Author(s):  
Urley Adrian Pérez-Moncada ◽  
María Margarita Ramírez-Gómez ◽  
Víctor Manuel Núñez-Zarante ◽  
Marcela Franco-Correa ◽  
Gabriel Roveda-Hoyos
Keyword(s):  
Mycorrhizal Fungi ◽  
Culture System ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Dry Weight ◽  
Extraradical Mycelium ◽  
Direct Inoculation ◽  
Autotrophic Culture ◽  
Glomus Sp

<strong>Objective</strong>. Obtain an in vitro mycorrhization system in autotrophic culture systems of blackberry plants (Rubus glaucus, Benth). <strong>Materials and methods</strong>. We used spores and root fragments with vesicles of Arbuscular Mycorrhizal Fungus (AMF) Glomus sp (GEV02). We established an autotrophic culture system of blackberry plantlets comparing two methods of direct inoculation of the AMF. We measured the number of spores produced, the length of the extraradical mycelium as well as the percentage of colonization of the AMF. Additionally, we measured the shoot and root length, and the fresh and dry weight of the leaf and root parts to determine the plant development. <strong>Results</strong>. The autotrophic culture system was successful for blackberry plants (Rubus glaucus, Benth; an optimal shoot and root growth was observed. Additionally, we obtained a system that allowed the development of Glomus sp. in in vitro conditions, with the formation of structures typical of the symbiosis as well as a good intraradical colonization, with the production of arbuscules and vesicles, development of extraradical mycelium with branched hyphae, and formation of new spores. <strong>Conclusion</strong>. For the first time, micropropagated blackberry plants associated successfully with an AMF under in vitro conditions, enabling the development of the symbiotic system AMF Glomus sp. associated to roots of micropropagated blackberry plantlets.<br /><strong>Key words</strong>: arbuscular mycorrhizal fungi (AMF), autotrophic culture, Rubus glaucus Benth, Glomus sp. (GEV02), in vitro mycorrhization.

Supplementation of c-type natriuretic peptide during in vitro growth period benefits the development of murine preantral follicles

Zygote ◽  
2020 ◽  
pp. 1-5
Author(s):  
Li Ang ◽  
Cao Haixia ◽  
Li Hongxia ◽  
Li Ruijiao ◽  
Guo Xingping ◽  
...  
Keyword(s):  
Natriuretic Peptide ◽  
Granulosa Cells ◽  
Culture System ◽  
Early Stage ◽  
Developmental Competence ◽  
Control Group ◽  
Follicle Development ◽  
Control Groups ◽  
Preantral Follicles

Summary The present study investigated the effects of c-type natriuretic peptide (CNP) on the development of murine preantral follicles during in vitro growth (IVG). Preantral follicles isolated from ovaries of Kunming mice were cultured in vitro. In the culture system, CNP was supplemented in the experimental groups and omitted in the control groups. In Experiment 1, CNP was only supplemented at the early stage and follicle development was evaluated. In Experiments 2 and 3, CNP was supplemented during the whole period of in vitro culture. In Experiment 2, follicle development and oocyte maturity were evaluated. In Experiment 3, follicle development and embryo cleavage after in vitro fertilization (IVF) were assessed. The results showed that in the control groups in all three experiments, granulosa cells migrated from within the follicle and the follicles could not reach the antral stage. In the experimental groups in all three experiments, no migration of granulosa cells was observed and follicle development was assessed as attaining the antral stage, which was significantly superior to that of the control group (P < 0.0001). Oocyte meiotic arrest was effectively maintained, hence giving good developmental competence. In conclusion, CNP supplementation in the culture system during IVG benefited the development of murine preantral follicles.

Morphometric Registration of Cytotoxic Changes of Epithelial Cells In Vitro: A Methodological Study

1990 ◽  
Vol 17 (3) ◽  
pp. 174-176
Author(s):  
Lis Andersen ◽  
Dorthe Arenholt-Bindslev
Keyword(s):  
Culture System ◽  
Optimal Allocation ◽  
Volume Density ◽  
Cell Culture System ◽  
Allocation Of Resources ◽  
Methodological Study ◽  
Epithelial Cell Culture ◽  
Coefficients Of Variation ◽  
And Control

Quantification of toxicity-induced cytomorphological effects in an epithelial cell culture system is described. Estimates of volume density and star volume of mitochondria and lysosomes are given. Mean volumes (n = 5) and coefficients of variation of these parameters were equal in experimental (TPA-treatment) and control cultures. An optimal allocation of resources for estimating cytomorphometric parameters would be to increase the number of culture flasks.

A Phytoalexin and Aflatoxin Producing Peanut Seed Culture System

1994 ◽  
Vol 21 (2) ◽  
pp. 130-134 ◽  
Author(s):  
S. M. Basha ◽  
R. J. Cole ◽  
S. K. Pancholy
Keyword(s):  
Water Stress ◽  
Culture System ◽  
Fungal Growth ◽  
Aflatoxin Contamination ◽  
Peanut Seed ◽  
Seed Culture ◽  
Murashige And Skoog Medium ◽  
Yellow Orange ◽  
Increased Susceptibility

Abstract An in vitro seed culture system was established to grow peanut seed of different maturities viz. white, yellow, orange, brown and black, using a modified Murashige and Skoog medium. Under this system peanut seed of yellow, orange, brown and black maturity categories grew to maturity as measured by increase in their size and germinability. In vitro cultured seeds produced significant amounts of phytoalexins and were contaminated with aflatoxins following their inoculation with Aspergillus spp. while the noninoculated sterile controls did not produce any phytoalexins. Exposure of seed cultures to water stress using various concentrations of mannitol (0 to 1 M) and polyethylene glycol 8000 (0-30% w/v) caused a significant decrease in their phytoalexin producing ability, and enhanced fungal growth compared to the nonstressed controls. The seeds that were stressed with mannitol and subsequently inoculated with A. flavus and A. parasiticus showed a significant increase in the aflatoxin contamination of stressed seed compared to the unstressed control. This would indicate that in vitro grown seeds responded to water stress similar to the field grown peanuts by loosing their ability to produce phytoalexins and increased susceptibility to aflatoxin contamination. Hence, this system has a potential application in evaluating peanut genotypes for aflatoxin resistance under water stress.

Optimized culture system to maximize ovarian cell growth and functionality in vitro

2021 ◽  
Author(s):  
Myung Jae Jeon ◽  
Young Sik Choi ◽  
James J. Yoo ◽  
Anthony Atala ◽  
John D. Jackson
Keyword(s):  
Cell Growth ◽  
Culture System ◽  
Ovarian Cell

scholarly journals Adipose and Muscle Cell Co-Culture System: A Novel In Vitro Tool to Mimic the In Vivo Cellular Environment

Biology ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 6
Author(s):  
Palaniselvam Kuppusamy ◽  
Dahye Kim ◽  
Ilavenil Soundharrajan ◽  
Inho Hwang ◽  
Ki Choon Choi
Keyword(s):  
Drug Development ◽  
Culture System ◽  
Cell Types ◽  
Culture Cell ◽  
In Vitro Techniques ◽  
Culture Systems ◽  
Complex Interactions ◽  
Cellular Environment

A co-culture system allows researchers to investigate the complex interactions between two cell types under various environments, such as those that promote differentiation and growth as well as those that mimic healthy and diseased states, in vitro. In this paper, we review the most common co-culture systems for myocytes and adipocytes. The in vitro techniques mimic the in vivo environment and are used to investigate the causal relationships between different cell lines. Here, we briefly discuss mono-culture and co-culture cell systems and their applicability to the study of communication between two or more cell types, including adipocytes and myocytes. Also, we provide details about the different types of co-culture systems and their applicability to the study of metabolic disease, drug development, and the role of secretory factors in cell signaling cascades. Therefore, this review provides details about the co-culture systems used to study the complex interactions between adipose and muscle cells in various environments, such as those that promote cell differentiation and growth and those used for drug development.

In vitro assessment of food-derived-glucose bioaccessibility and bioavailability in bicameral cell culture system

2020 ◽  
Vol 45 (5) ◽  
pp. 631-637
Author(s):  
Cansu Ozel-Tasci ◽  
Gozde Pilatin ◽  
Ozgur Edeer ◽  
Sukru Gulec
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Metabolic Diseases ◽  
Enzymatic Digestion ◽  
Cell Culture System ◽  
Culture Studies ◽  
Experimental Approaches ◽  
In Vitro Assessment

AbstractBackgroundFunctional foods can help prevent metabolic diseases, and it is essential to evaluate functional characteristics of foods through in vitro and in vivo experimental approaches.ObjectiveWe aimed to use the bicameral cell culture system combined with the in vitro digestion to evaluate glucose bioavailability.Materials and methodsCake, almond paste, and pudding were modified by adding fiber and replacing sugar with sweeteners and polyols. Digestion process was modeled in test tubes. Rat enterocyte cells (IEC-6) were grown in a bicameral cell culture system to mimic the physiological characteristics of the human intestine. The glucose bioaccessibility and cellular glucose efflux were measured by glucose oxidase assay.Results and discussionThe glucose bioaccessibilities of modified foods were significantly lower (cake: 2.6 fold, almond paste: 9.2 fold, pudding 2.8 fold) than the controls. Cellular glucose effluxes also decreased in the modified cake, almond paste, and pudding by 2.2, 4, and 2 fold respectively compared to their controls.ConclusionOur results suggest that combining in vitro enzymatic digestion with cell culture studies can be a practical way to test in vitro glucose bioaccessibility and bioavailability in functional food development.

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