Pulsed field gel electrophoresis (PFGE) analysis for multidrug resistant Salmonella enterica serovar Schwarzengrund isolates collected in six years (2000–2005) from retail chicken meat in Taiwan

2011 ◽  
Vol 28 (3) ◽  
pp. 399-405 ◽  
Author(s):  
Ming-Hui Chen ◽  
Wen-Zhe Hwang ◽  
Shu-Wan Wang ◽  
Yang-Chih Shih ◽  
Hau-Yang Tsen
Keyword(s):  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Multidrug Resistant ◽  
Pulsed Field Gel Electrophoresis ◽  
Chicken Meat ◽  
Pfge Analysis ◽  
Pulsed Field ◽  
Retail Chicken Meat

scholarly journals Multiple Clones within Multidrug-ResistantSalmonella enterica Serotype Typhimurium Phage Type DT104

2000 ◽  
Vol 38 (3) ◽  
pp. 1269-1271 ◽  
Author(s):  
Antonis Markogiannakis ◽  
Panayotis T. Tassios ◽  
Maria Lambiri ◽  
Linda R. Ward ◽  
Jenny Kourea-Kremastinou ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Phage Type ◽  
Antibiotic Resistance Genes ◽  
Multidrug Resistant ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

Six distinct clones were present among Greek multidrug-resistantSalmonella enterica serotype Typhimurium phage type DT104, since isolates belonging to resistance phenotypes including the ACSSuT (ampicillin, chloramphenicol, streptomycin, sulfonamides, and tetracycline) core could be distinguished with respect to their pulsed-field gel electrophoresis patterns, int1 integron structures, and presence or absence of antibiotic resistance genesant(3")-Ia, pse-1, and tem-1.

Molecular Characterization of Salmonella Paratyphi B dT+ and Salmonella Heidelberg from Poultry and Retail Chicken Meat in Colombia by Pulsed-Field Gel Electrophoresis

2015 ◽  
Vol 78 (4) ◽  
pp. 802-807 ◽  
Author(s):  
PILAR DONADO-GODOY ◽  
BARBARA A. BYRNE ◽  
MICHAEL HUME ◽  
MARIBEL LEÓN ◽  
ENRIQUE PÉREZ-GUTIÉRREZ ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Chicken Meat ◽  
Poultry Production ◽  
Chromosomal Dna ◽  
Production Chain ◽  
Pulsed Field ◽  
Paratyphi B ◽  
Retail Chicken Meat ◽  
Salmonella Heidelberg

Salmonella Paratyphi B dT+ variant (also termed Salmonella Java) and Salmonella Heidelberg are pathogens of public health importance that are frequently isolated from poultry. As a step toward implementing the Colombian Integrated Program for Antimicrobial Resistant Surveillance, this study characterized molecular patterns of Salmonella Paratyphi B dT+and Salmonella Heidelberg isolated from poultry farms, fecal samples, and retail chicken meat using pulsed-field gel electrophoresis (PFGE). The objective of this study was to determine the genetic relationship among isolates and to determine potential geographically predominant genotypes. Based on PFGE analysis, both serovars exhibited high heterogeneity: the chromosomal DNA fingerprints of 82 Salmonella Paratyphi B dT+ isolates revealed 42 PFGE patterns, whereas the 21 isolates of Salmonella Heidelberg revealed 10 patterns. Similar genotypes of both serovars were demonstrated to be present on farms and in retail outlets. For Salmonella Paratyphi B dT+, closely genetically related strains were found among isolates coming from different farms and different integrated poultry companies within two departments (Santander and Cundinamarca) and also from farms located in the two geographically distant departments. For Salmonella Heidelberg, there were fewer farms with genetically related isolates than for Salmonella Paratyphi B dT+. A possible dissemination of similar genotypes of both serovars along the poultry production chain is hypothesized, and some facilitating factors existing in Colombia are reviewed.

scholarly journals Application of phage typing and pulsed-field gel electrophoresis to analyse Salmonella enterica isolates from a suspected outbreak in Lagos, Nigeria

2010 ◽  
Vol 4 (12) ◽  
pp. 828-833 ◽  
Author(s):  
Kabiru Olusegun Akinyemi ◽  
Werner Philipp ◽  
Wolfgang Beyer ◽  
Reinhard Böhm
Keyword(s):  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Salmonella Enteritidis ◽  
Phage Type ◽  
Multidrug Resistant ◽  
Human Infection ◽  
Pulsed Field Gel Electrophoresis ◽  
Phage Typing ◽  
Pfge Type ◽  
Pulsed Field

Introduction: Inadequate potable water supply and poor sanitation predispose to food- and water-borne diseases associated with Salmonella enterica serovars in developing countries. In this study the possible source of an unprecedented upsurge of Salmonella-associated community gastroenteritis was traced using both phage-typing and pulsed-field gel electrophoresis (PFGE). Methodology: Nineteen Salmonella Typhimurium (three sporadic isolates included) and 13 Salmonella Enteritidis isolates from clinical, animal, and environmental samples were subjected to antimicrobial susceptibility testing, phage-typing, and PFGE analysis using standard procedures. Results: Eleven (68.8%) of the 16 outbreak-related multidrug resistant S. Typhimurium belonged to DT 71 phage type with cluster PFGE type X3, representing the most prevalent strain identified among human, animal, and environmental isolates. The remaining five (31.2%) outbreak-related strains  reacted but did not conform with clear phage types (RDNC) with cluster PFGE types X1 and X2 (96.8% similarity). Sporadic strains were untypable and belonged to X4 PFGE type. However, the evaluated S. Enteritidis strains that were multidrug resistant without a definite phage type belonged to PFGE cluster type X1e and were identified among the water and human strains. None of the Typhimurium and Enteritdis isolates was resistant to the fluoroquinolone antibiotics that were evaluated. Conclusion: This study emphasizes the epidemiological usefulness of PFGE typing in the detection of emerging strains of multipledrug resistant Salmonella, particularly S. Typhimurium DT71, that pose serious health implications in our environment. The study provides epidemiological links between environmental reservoirs and human infection in this community.

scholarly journals Typing of Salmonella enterica Serotype Paratyphi C Isolates from Various Countries by Plasmid Profiles and Pulsed-Field Gel Electrophoresis

1999 ◽  
Vol 37 (6) ◽  
pp. 2058-2060 ◽  
Author(s):  
S. Kariuki ◽  
J. Cheesbrough ◽  
A. K. Mavridis ◽  
C. A. Hart
Keyword(s):  
Restriction Endonuclease ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Multidrug Resistant ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Plasmid Profiles ◽  
Similar Restriction ◽  
Restriction Endonuclease Digest ◽  
Pfge Profiles

Pulsed-field gel electrophoresis (PFGE) of 61 Salmonella enterica serotype Paratyphi C isolates from six countries gave five distinct clusters. Twenty-four isolates from five countries were susceptible to 10 antimicrobials tested and gave similar restriction endonuclease digest patterns of the 38-MDa plasmid. In contrast, plasmid and PFGE profiles of 37 multidrug-resistant isolates from Zaire were different from those from other countries.

scholarly journals Molecular Analyses of Salmonellaenterica Isolates from Fish Feed Factories and Fish Feed Ingredients

2003 ◽  
Vol 69 (2) ◽  
pp. 1075-1081 ◽  
Author(s):  
Live L. Nesse ◽  
Kerstin Nordby ◽  
Even Heir ◽  
Bjarne Bergsjoe ◽  
Traute Vardund ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Profile Analysis ◽  
Plasmid Profile ◽  
Pulsed Field Gel Electrophoresis ◽  
Fish Feed ◽  
Molecular Analyses ◽  
Feed Ingredients ◽  
Pulsed Field ◽  
Salmonella Serovars

ABSTRACT Isolates of the most commonly observed salmonella serovars in Norwegian fish feed factories from 1998 to 2000 (Salmonella enterica serovar Agona, S. enterica serovar Montevideo, S. enterica serovar Senftenberg, and S. enterica serovar Kentucky) were studied by pulsed-field gel electrophoresis (PFGE) and plasmid profile analysis and compared to isolates of the same serovars from fish feed ingredients, humans, and other sources (a total of 112 isolates). Within each serovar, a variety of distinct PFGE types (with similarity levels less than 90%) were observed in the feed ingredients and other sources, while only two distinct types of each serovar were identified in the factories. The combined results of PFGE and plasmid analyses showed that each factory harbored only a few S. enterica clones. Some of these clones persisted for at least 3 years in the factories, indicating that there was long-lasting contamination probably due to inadequate decontamination procedures.

scholarly journals Outbreak of Salmonella enterica serotype Muenster infections associated with goat's cheese, France, March 2008

2009 ◽  
Vol 14 (31) ◽  
Author(s):  
D van Cauteren ◽  
N Jourdan-da Silva ◽  
F X Weill ◽  
L King ◽  
A Brisabois ◽  
...  
Keyword(s):  
Food Consumption ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Pulsed Field Gel Electrophoresis ◽  
Gastrointestinal Illness ◽  
Pulsed Field

Salmonella enterica serotype Muenster (hereafter referred to as S. Muenster) is rare in France and in Europe. In France, a nationwide outbreak of gastrointestinal illness due to S. Muenster occurred during March and April 2008. Twenty-five laboratory-confirmed cases of S. Muenster were documented by telephone using a trawling questionnaire. Four patients were admitted to hospital and no death was recorded. Among the 21 interviewed cases, 16 reported consumption of goat's cheese in the days prior to symptoms. The investigation incriminated goat's cheese from producer X as being the most likely source of the outbreak. S. Muenster was isolated from both cases and the incriminated goat's cheese. The pulsed-field gel electrophoresis profiles of the food isolates of producer X and the isolates from cases were indistinguishable. Following the withdrawal of the contaminated batch of cheese, the number of cases decreased to its usual level. To our knowledge, this is the first published outbreak of S. Muenster associated with food consumption in Europe.

scholarly journals Nontyphoidal Salmonellae in United Kingdom Badgers: Prevalence and Spatial Distribution

2003 ◽  
Vol 69 (7) ◽  
pp. 4312-4315 ◽  
Author(s):  
J. Sian Wilson ◽  
Sarah M. Hazel ◽  
Nicola J. Williams ◽  
Amos Phiri ◽  
Nigel P. French ◽  
...  
Keyword(s):  
Spatial Distribution ◽  
United Kingdom ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Social Groups ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

ABSTRACT Eighteen (72%) of 25 badger social groups were found to excrete Salmonella enterica serovar Ried, S. enterica serovar Binza, S. enterica serovar Agama, or S. enterica serovar Lomita. Each serovar was susceptible to a panel of antimicrobials. Based on results of pulsed-field gel electrophoresis, the S. enterica serovar Agama and S. enterica serovar Binza isolates were very similar, but two clones each of S. enterica serovar Lomita and S. enterica serovar Ried were found. Badgers excreting S. enterica serovar Agama were spatially clustered.

Antimicrobial Resistance of Salmonella enterica Isolates from Bulk Tank Milk and Milk Filters in the United States†

2013 ◽  
Vol 76 (1) ◽  
pp. 18-25 ◽  
Author(s):  
J. S. VAN KESSEL ◽  
J. SONNIER ◽  
S. ZHAO ◽  
J. S. KARNS
Keyword(s):  
Antimicrobial Resistance ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Pulsed Field Gel Electrophoresis ◽  
Class I ◽  
Bulk Tank Milk ◽  
Pulsed Field ◽  
Extended Spectrum ◽  
Class I Integrons ◽  
Bulk Tank

Salmonella isolates were recovered from bulk tank milk as part of the National Animal Health Monitoring System (NAHMS) Dairy 2002 and 2007 surveys. In-line milk filters were also tested in the 2007 survey. The objective of this study was to determine the prevalence of antimicrobial resistance among Salmonella enterica isolates from bulk milk and milk filters in the NAHMS Dairy 2002 and 2007 surveys and to further characterize resistant isolates. Susceptibilities to 15 antibiotics were determined for 176 Salmonella isolates of 26 serotypes using an automated antimicrobial susceptibility system. Resistant isolates were screened by PCR for the presence of the extended-spectrum β-lactamase (blaCMY) gene and class I integrons and further characterized by pulsed-field gel electrophoresis. Thirty isolates (17.0%) representing six S. enterica serotypes exhibited resistance to at least one antimicrobial agent (serotypes Newport [14 of 14 isolates exhibited resistance], Dublin [7 of 7], Typhimurium [3 of 5], Kentucky [4 of 22], Anatum [1 of 13], and Infantis [1 of 2]). Twenty isolates (11.4%), including all 14 Newport, 3 Dublin, 2 Typhimurium, and 1 Infantis isolate, displayed the typical multidrug-resistant, blaCMY-positive (MDR-AmpC) phenotype which included resistance to ampicillin, chloramphenicol, streptomycin, sulfonamide, and tetracycline, plus resistance to amoxicillin–clavulanic acid and extended-spectrum cephalosporins. Five of the MDR-AmpC isolates carried class I integrons (2.8%). Two-enzyme (XbaI and BlnI) pulsed-field gel electrophoresis discerned clades within serotypes and, together with the resistance profiles, identified strains that appeared to have persisted temporally and geographically. These results suggest that there is a low but appreciable risk of infection with MDR Salmonella from consumption of nonpasteurized milk and dairy products.

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