Studies on the antioxidant activities of cinnamon (Cinnamomum verum) bark extracts, through various in vitro models

In the present work methanolic and water extracts of 19 medicinal plants, commonly used in Sudanese folk medicines against gastrointestinal tract, infectious diseases were investigated for their antioxidant activity. Antioxidant assays were carried out by using different in vitro models such as DPPH (2, 2-diphenyl-1picrylhydrazyl) radical scavenging and Iron chelating activity. Methanolic and water extracts of Punica granatum showed the highest total antioxidant activity of 71±0.03 and 73±0.01 respectively. The antioxidant activities of water extracts were poor when compared to the methanol extract. The iron chelating power of methanol and aqueous extracts of Commiphora myrrha 58±0.01 and Azadirachta indica 57±0.20 respectively.

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Antinociceptive and Antioxidant Activities of Phytol In Vivo and In Vitro Models

Neuroscience Journal ◽

10.1155/2013/949452 ◽

2013 ◽

Vol 2013 ◽

pp. 1-9 ◽

Cited By ~ 79

Author(s):

Camila Carolina de Menezes Patrício Santos ◽

Mirian Stiebbe Salvadori ◽

Vanine Gomes Mota ◽

Luciana Muratori Costa ◽

Antonia Amanda Cardoso de Almeida ◽

...

Keyword(s):

Antioxidant Activities ◽

Antioxidant Properties ◽

Thiobarbituric Acid ◽

In Vitro Models ◽

Control Group ◽

Second Phase ◽

Hot Plate Test ◽

Antinociceptive Effects

The objective of the present study was to evaluate the antinociceptive effects of phytol using chemical and thermal models of nociception in mice and to assess its antioxidant effects in vitro. Phytol was administered intraperitoneally (i.p.) to mice at doses of 25, 50, 100, and 200 mg/kg. In the acetic acid-induced writhing test, phytol significantly reduced the number of contortions compared to the control group (P<0.001). In the formalin test, phytol reduced significantly the amount of time spent in paw licking in both phases (the neurogenic and inflammatory phases), this effect being more pronounced in the second phase (P<0.001). Phytol also provoked a significant increase in latency in the hot plate test. These antinociceptive effects did not impaire the motor performance, as shown in the rotarod test. Phytol demonstrated a strong antioxidant effect in vitro in its capacity to remove hydroxyl radicals and nitric oxide as well as to prevent the formation of thiobarbituric acid reactive substances (TBARS). Taken as a whole, these results show the pronounced antinociceptive effects of phytol in the nociception models used, both through its central and peripheral actions, but also its antioxidant properties demonstrated in the in vitro methods used.

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Novel Coupled Molecules from Active Structural Motifs of Synthetic and Natural Origin as Immunosuppressants

Medicinal Chemistry ◽

10.2174/1573406415666190409111459 ◽

2020 ◽

Vol 16 (4) ◽

pp. 544-554

Author(s):

Richa Minhas ◽

Gulshan Bansal ◽

Yogita Bansal

Keyword(s):

Antioxidant Activities ◽

Organ Transplant ◽

Docking Studies ◽

In Vitro Models ◽

Autoimmune Disorders ◽

Docking Score ◽

Nbt Reduction ◽

Inos Inhibition ◽

And Control

Introduction: Nitric oxide (NO) is an important mediator in the pathogenesis and control of immune system-related disorders and its levels are modulated by inducible NO synthase (iNOS). Oxidative stress is another pathological indication in majority of autoimmune disorders. The present study aims at the development of coupled molecules via selection of pharmacophores for both immunomodulatory and antioxidant activities through iNOS inhibition. Methods: Variedly substituted coumarin moieties are coupled with naturally occurring phenols through an amide linkage and were predicted for activities using computer-based program PASS. The compounds predicted to have dual activities were synthesized. Docking studies were carried out against iNOS (PDB 1R35) and compounds having good docking score were evaluated for immunomodulatory and antioxidant activities. Results: The synthesized compounds were found to be pure and were obtained in good yields. Compounds with maximum docking score (YR1a, YR2e, YR2c and YR4e) were selected for evaluation by in vitro models. Compounds YR2e and YR2c markedly inhibited the reduction of NBT dye and showed maximum % iNOS inhibition. In DPPH assay, compound YR4e was observed as the most potent antioxidant (EC50 0.33 µM/mL). Based on these studies, compounds YR2e and YR2c were selected for haemagglutination test. Compound YR2e was observed as the most active immunosuppressant with maximal inhibitory ability of iNOS and NBT reduction and lower HAT value of 3.5. Conclusion: Compound YR2e can be utilized as a pharmacological agent in the prevention or treatment of immunomodulatory diseases such as tumors, rheumatoid arthritis, ulcerative colitis, organ transplant and other autoimmune disorders.

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Nrf2/HO-1 Mediated Antioxidant Activities, Cytotoxicity Analysis and LCESI/ MS Profiling of Eulophia nuda L.

The Natural Products Journal ◽

10.2174/2210315509666190215101646 ◽

2020 ◽

Vol 10 (1) ◽

pp. 69-79 ◽

Cited By ~ 1

Author(s):

Vikas Nanekar ◽

Varsha Shriram ◽

Tushar Khare ◽

Vinay Kumar

Keyword(s):

Free Radical ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Fold Increase ◽

In Vitro Models ◽

Free Radical Scavenging ◽

Related Factor ◽

Mcf7 Cells ◽

Esi Ms

Background: Eulophia nuda, is a highly medicinal orchid with strong antioxidant and anticancer potentials in traditional systems of medicine. But few reports are available on the scientific validation. The aim of the study was to investigate phytochemical constituents, antioxidant and cytotoxic efficacies of extracts of Eulophia nuda, and the underlying mechanisms-of-action via upregulation of nuclear transcription factor-erythroid-2 related factor (Nrf2) and hemeoxygenase-1 (HO-1) pathways. Methods: Petroleum Ether (PEE), Ethyl Acetate (EAE), Methanol (ME) and Aqueous Methanol (AqME) extracts of shade dried tubers were obtained and concentrated in vacuo. Total phenols, flavonoids, condensed tannins, ascorbic acid and carotenoids were estimated from the extracts using standard methods. Antioxidant activities of extracts were determined by total antioxidant, FRAP, ABTS, DPPH, OH, H2O2, NO, O2 ·- radical scavenging assays. Cytotoxicity of EAE and ME were assessed against MCF7 cells in vitro. LC-ESI/MS profiling of EAE was carried out. Quantitative Real-Time (qRT) PCR was used for the expression analysis of Nrf2 and HO1 genes in EAE-treated MCF7 cells. Results: In vitro models confirmed strong dose-dependent antioxidant and free-radical scavenging potencies of E. nuda tuber extracts. Overall antioxidant efficacies were in the order EAE > ME > AqME > PEE. EAE showed striking cytotoxicity followed by ME (0.86% and 5.17% cell survival at 1000 µg ml-1, respectively). LC-ESI/MS profiling of most potent extract EAE revealed 37 identified compounds including catechin, taxifolin, tocopherol, trigallic acid and chlorogenic acid, all known for their strong antioxidant/anticancer properties. Expression levels of Nrf2 and HO1 genes were up-regulated in MCF7 cells beyond 50 μg ml-1 extract concentration with > 2-fold increase at 200 µg ml-1 EAE. Conclusion: The data demonstrated that E. nuda extracts possess strong free radical scavenging and antioxidant efficacies and the mechanism of action may be via inducing Nrf2 and HO-1.

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Antisteatotic and antioxidant activities of Thymbra spicata L. extracts in hepatic and endothelial cells as in vitro models of non-alcoholic fatty liver disease

Journal of Ethnopharmacology ◽

10.1016/j.jep.2019.111919 ◽

2019 ◽

Vol 239 ◽

pp. 111919 ◽

Cited By ~ 8

Author(s):

Mohamad Khalil ◽

Hala Khalifeh ◽

Francesca Baldini ◽

Annalisa Salis ◽

Gianluca Damonte ◽

...

Keyword(s):

Endothelial Cells ◽

Liver Disease ◽

Fatty Liver ◽

Fatty Liver Disease ◽

Antioxidant Activities ◽

In Vitro Models ◽

Alcoholic Fatty Liver ◽

Thymbra Spicata ◽

Alcoholic Fatty Liver Disease

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In vitro antioxidant and anti-inflammatory activities of the chloroform extract of Combretum punctatum var squamosum

Science Vision ◽

10.33493/scivis.19.03.04 ◽

2019 ◽

Vol 19 (3) ◽

pp. 100-108

Author(s):

K. Thanzami ◽

B.B. Kakoti ◽

C. Lalremruati

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Chloroform Extract ◽

In Vitro Models ◽

Percent Inhibition ◽

Anti Inflammatory

The objective of this study was to investigate the antioxidant and anti-inflammatory activities of the chloroform extract of Combretum punctatum var squamosum by in vitro models. Antioxidant activity was determined by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, reducing power and lipid peroxidation assays. The phenolic and flavonoid contents of the extract were also assessed. The extract at different concentrations showed significant percent inhibition of DPPH and lipid peroxidation and high reducing power when compared with the standard. The extract also showed a significantly high content of phenolics and flavonoids. Anti-inflammatory activity was studied by inhibition of albumin denaturation and human red blood cell (HRBC) membrane stabilization methods. The extract at different concentrations showed significant percent inhibition of albumin denaturation and percent inhibition of haemolysis when compared with the standard. Thus, the result indicates that the chloroform extract exhibited significant potential on anti-inflammatory and antioxidant activities.

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Comparison of phenolic content and antioxidant activity of two common fruits of Bangladesh in solvents of varying polarities

Food Research ◽

10.26656/fr.2017.5(6).253 ◽

2021 ◽

Vol 5 (6) ◽

pp. 187-196

Author(s):

R. Ghosh ◽

P. Barua ◽

O. Sikder ◽

S. Saha ◽

S. Mojumder ◽

...

Keyword(s):

Antioxidant Activity ◽

Phenolic Content ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Reducing Power ◽

In Vitro Models ◽

Frap Assay ◽

Reducing Power Assay ◽

Dpph Scavenging

Phenolic content and antioxidant activity of two common fruits of Bangladesh, namely Phyllunthus emblica and Elaeocarpus floribundus, were measured in water, methanol, ethanol, acetone and hexane extracts. Several in vitro models including phosphomolybdenum assay, DPPH free radical scavenging assay, FRAP assay and reducing power assay were used to assess the antioxidant activity of these extracts in comparison with reference antioxidants. Between the two fruits, P. emblica showed higher phenolic content and antioxidant activity in all the solvents used. In the DPPH scavenging assay, the activity of P. emblica extracts was close to reference antioxidants, ascorbic acid and BHT. Besides, considering the solvents used, extracts of both fruits had the highest phenolic and antioxidant activity in polar solvents. The correlation coefficient between total phenolics and antioxidant activities was found statistically significant. These findings suggest that P. emblica could be an excellent antioxidant resource for industries like food, pharmaceutical, and cosmetics.

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Antimalarial, Antioxidant Activities and Chemoprofile of Sansevieria liberica Gerome and Labroy (Agavaceae) Leaf Extract

Advances in Pharmacological and Pharmaceutical Sciences ◽

10.1155/2021/9053262 ◽

2021 ◽

Vol 2021 ◽

pp. 1-11

Author(s):

Ifeoma Celestina Orabueze ◽

Sarah Chiamaka Uzor ◽

Bou Ndiaye ◽

Desire Uba ◽

Duncan Azubuike. Ota ◽

...

Keyword(s):

Vitamin C ◽

High Performance ◽

Leaf Extract ◽

Antioxidant Activities ◽

Folk Medicine ◽

Reducing Power ◽

In Vitro Models ◽

Phytochemical Analysis ◽

Perennial Plant

Background. Sansevieria liberica Gerome and Labroy (Agavaceae) is a religious and ornamental perennial plant with highly valued medicinal usage in Nigeria. Sansevieria liberica is used in the management of malarial fever. The ease of development of resistance to available antimalarial drugs has resulted in increased clinical failure and mortality. The study investigated the antimalarial effects of Sansevieria liberica (SL) leaf extract in mice infected with Plasmodium berghei. Materials and Methods. The ability of SL leaf extract to suppress the growth of malaria parasites in early (suppressive) and established (curative) infections was established using animal models. The mean survival time (MST) was determined. The antioxidant potential was established using two standard in vitro models. High-performance liquid chromatography (HPLC) and phytochemical analysis methods were used to create a chemoprofile fingerprint of SL hydroethanolic leaf extract. Results. At 200, 400, and 400·mg kg−1, SL produced 68.71, 70.74, and 75.09% parasite suppression in the suppressive model while the curative model gave a percentage of cure of 71.09, 72.60, and 62.09, respectively. The animals lived longer compared to both negative and positive controls but were not fully protected. The IC50 values of SL and vitamin C were calculated to be 3.599 µg mL−1 and 3.08 µg mL−1, respectively. The reducing power of vitamin C was significantly ( P < 0.05 ) higher than that of SL extract. Some flavonoids were established as possible marker compounds for SL leaf extract. Discussion and Conclusions. The antimalarial assay results demonstrated that the use of SL in folk medicine may have scientific support.

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