Characterisation of thermostable trypsin and determination of trypsin isozymes from intestine of Nile tilapia (Oreochromis niloticus L.)

2012 ◽  
Vol 134 (3) ◽  
pp. 1533-1541 ◽  
Author(s):  
Sasimanas Unajak ◽  
Piyachat Meesawat ◽  
Atchara Paemanee ◽  
Nontawith Areechon ◽  
Arunee Engkagul ◽  
...  
2011 ◽  
Vol 31 (2) ◽  
pp. 361-365 ◽  
Author(s):  
Alexandre Nobuhiro Tajiri ◽  
Daisy Pontes Netto ◽  
Márcia Sassahara ◽  
Mari Sylmara Martins Rodrigues ◽  
Cristina Arduini Cavalcanti de Arruda

2019 ◽  
Vol 8 (3) ◽  
pp. 242-249
Author(s):  
Dana Yuli Agustina ◽  
Djoko Suprapto ◽  
Sigit Febrianto

ABSTRAK Timbal merupakan logam berat yang bersifat toksik dan biasanya bersumber dari industri cat, baterai dan percetakan. Sungai Tenggang berdekatan dengan Lingkungan Industri Kecil (LIK) dan pemukiman sehingga meningkatkan limbah ke dalam perairan termasuk logam berat. Hal tersebut menjadi alasan mengapa penelitian mengenai kandungan logam berat timbal pada ikan nila (Oreochromis niloticus) di Sungai tenggang, Semarang, Jawa Tengah ini dilaksanakan. Penelitian ini bertujuan untuk mengetahui kandungan logam berat timbal pada air dan ikan nila, mengetahui nilai biokonsentrasi faktor ikan nila serta mengetahui batas konsumsi mingguan daging ikan nila. Penelitian ini dilaksanakan pada bulan April 2019. Metode yang digunakan adalah metode deskriptif dengan pendekatan secara kuantitatif serta sampling menggunakan metode purposive sampling. Hasil perhitungan konsentrasi logam berat Pb dalam air rata-rata sebesar 0,155 mg/l. Konsentrasi logam berat Pb dalam daging ikan nila rata-rata 2,35 mg/kg. Hasil perhitungan bioconcentration factor (BCF) berkisar antara 12,03 – 17,17.  Hasil perhitungan berat maksimal konsumsi ikan nila mingguan diperoleh rata-rata sebesar 0,63 kg/minggu. Berdasarkan hasil yang telah diperoleh dapat disimpulkan bahwa kadar konsentrasi logam berat timbal pada air dan ikan nila di Sungai Tenggang tergolong cukup tinggi serta sudah melebihi ambang batas baku mutu yang telah ditentukan. Angka BCF logam berat timbal pada ikan nila termasuk dalam kategori tingkat akumulatif rendah (BCF < 100). ABSTRACT Plumbum is heavy metal that is toxic and source from paint industry, battery and printing. Tenggang river near with small industrial environment (LIK) and settlement so that increasing the waste entering to river including heavy metal waste. This is the reason for this research do. This study aims to determine the heavy metal Pb of water and Nile tilapia meat, bioconcentration factor of nile and knowing the limit of weekly consumption of  nile tilapia meat (MTI). The study was conducted in April 2019. The method used was a descriptive method with the determination of sampling using purposive sampling. The result of heavy metal Pb concentration in water averaged by 0,155 mg/l. Concentration of heavy metal Pb in Nile tilapia (Oreochromis niloticus) meat averaged by 2,35 mg/kg. The calculation result of bioconcentration factor (BCF) is range between 12,03 – 17,17. The maximum limit result of weekly fish consumption is averaged by 0,63 kg/minggu. Based on the results obtained it can be concluded that concentration of heavy metal Pb in water and nile tilapia tenggang river quite high and has reached above the permisible limit. BCF number of heavy metal in nile tilapia fall into the category of low accumulative levels (BCF < 100).


2019 ◽  
Vol 18 (3) ◽  
Author(s):  
Andri Warsa ◽  
Lismining Pujiyani Astuti

A gillnet is a common fishing gear for exploitation and fish capture at Jatiluhur Reservoir. The fish size captured by the gillnet depends on the mesh size for its selective fishing gear. Determination of gillnet mesh size is one of effort for fisheries management.  The management of nile tilapia (Oreochromis niloticus) is based on gillnet selectivity and reproductive biology of nile tilapia. The aim of the research was to estimate length at the first mature (Lm) and gillnet selectivity for nile tilapia. The research was conducted at February-September 2017 through experimental fishing using gillnet with 1.0–4.0 mesh size (interval 0.5 inch). The gillnet installation was performed at six stations, namely Cihuni-Cibadak, Sungai Cikanyayan, Sungai Ciririp, Sungai Cihonje, Pasir Kole and Cilalawi. The Lm of nile tilapia was 18.3 and 23.3 cm for female and male, respectively. The total length retained in the gillnet with the highest probability for mesh size 1.0; 1.5; 2.0; 2.5; 3.0; 3.5 and 4.0 inches were 9.5; 12.5; 14.5; 18.5; 20.5; 26.5 and 30.5 cm, respectively. The minimum mesh size used for nile tilapia exploitation was ≥3 inches because it was able to catch nile tilapia with the total length larger than Lm (Lc>Lm) and with an SPR value of > 40% so that the tilapia resource in the Jatiluhur Reservoir remains sustainable.  


2016 ◽  
Vol 67 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Aysel Çağlan Karasu Benli ◽  
Belda Erkmen ◽  
Figen Erkoç

Abstract This study aimed to assess genotoxicity in Nile tilapia (Oreochromis niloticus) erythrocytes after exposure to a sub-lethal concentration of 10 mg L-1 di-n-butyl phthalate (DBP) for 24 and 96 h. The results showed that mean MN frequencies in both DBP and ethyl methane-sulfonate (EMS, positive control for MN bioassay) groups were significantly different (p<0.01) with respect to control and solvent control groups, in both exposure scenarios. When analysing nuclear abnormalities, the frequency of notched nuclei was significantly different (p<0.05) but the frequencies of other subtypes did not change. The 96-h exposure led to an increase in the mean frequencies of notched nuclei, and also caused significant differences between MN frequencies in all groups (p<0.01). Our findings indicate that sub-lethal DBP concentrations when tested in controlled laboratory conditions have genotoxic potential towards Nile tilapia. Further detailed studies should be done for the determination of the environmental risk assessment for aquatic life since DBP is a high risk contaminant of freshwater and marine ecosystems.


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