Quantitative benefit and risk assessment of arsenic and nutrient levels in cooked and raw chinese mitten crab (Eriocheir sinensis) using an in vitro digestion model

2021 ◽  
pp. 130826
Author(s):  
Fan zihao ◽  
Wang qian ◽  
Chen xi ◽  
Qiu liping ◽  
Yin Yuting ◽  
...  
Keyword(s):  
Risk Assessment ◽  
In Vitro Digestion ◽  
Eriocheir Sinensis ◽  
Chinese Mitten Crab ◽  
Nutrient Levels ◽  
Mitten Crab ◽  
Benefit And Risk

scholarly journals An inhibitor of apoptosis protein (EsIAP1) from Chinese mitten crab Eriocheir sinensis regulates apoptosis through inhibiting the activity of EsCaspase-3/7-1

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Chen Qu ◽  
Jiejie Sun ◽  
Qingsong Xu ◽  
Xiaojing Lv ◽  
Wen Yang ◽  
...  
Keyword(s):  
Caspase 3 ◽  
Hydrolytic Activity ◽  
Eriocheir Sinensis ◽  
Inhibitor Of Apoptosis ◽  
Chinese Mitten Crab ◽  
Apoptosis Pathway ◽  
Mitten Crab ◽  
Apoptosis Protein ◽  
Apoptosis Proteins

AbstractInhibitor of apoptosis proteins (IAPs) maintain the balance between cell proliferation and cell death by inhibiting caspase activities and mediating immune responses. In the present study, a homolog of IAP (designated as EsIAP1) was identified from Chinese mitten crab Eriocheir sinensis. EsIAP1 consisted of 451 amino acids containing two baculoviral IAP repeat (BIR) domains with the conserved Cx2 Cx6 Wx3 Dx5 Hx6 C motifs. EsIAP1 mRNA was expressed in various tissues and its expression level in hemocytes increased significantly (p < 0.01) at 12–48 h after lipopolysaccharide stimulation. In the hemocytes, EsIAP1 protein was mainly distributed in the cytoplasm. The hydrolytic activity of recombinant EsCaspase-3/7-1 against the substrate Ac-DEVD-pNA decreased after incubation with rEsIAP1. Moreover, rEsIAP1 could directly combine with rEsCaspase-3/7-1 in vitro. After EsIAP1 was interfered by dsRNA, the mRNA expression and the hydrolytic activity of EsCaspase-3/7-1 increased significantly, which was 2.26-fold (p < 0.05) and 1.71-fold (p < 0.05) compared to that in the dsGFP group, respectively. These results collectively demonstrated that EsIAP1 might play an important role in apoptosis pathway by regulating the activity of EsCaspase-3/7-1 in E. sinensis.

Establishment of spiroplasma-infected hemocytes as an in vitro laboratory culture model of Chinese mitten crab Eriocheir sinensis

2014 ◽  
Vol 171 (1-2) ◽  
pp. 215-220 ◽  
Author(s):  
Wei Gu ◽  
Wei Yao ◽  
Yang zhao ◽  
Siyu Pei ◽  
Chenkang Jiang ◽  
...  
Keyword(s):  
Eriocheir Sinensis ◽  
Laboratory Culture ◽  
Chinese Mitten Crab ◽  
Mitten Crab ◽  
Culture Model

ERK Is Involved in the Process of Acrosome Reaction In Vitro of the Chinese Mitten Crab, Eriocheir sinensis

2015 ◽  
Vol 17 (3) ◽  
pp. 305-316 ◽  
Author(s):  
Wen-Juan Sun ◽  
Ming Zhu ◽  
Yuan-Li Wang ◽  
Qing Li ◽  
Hong-Dan Yang ◽  
...  
Keyword(s):  
Acrosome Reaction ◽  
Eriocheir Sinensis ◽  
Chinese Mitten Crab ◽  
Mitten Crab

scholarly journals Effects of miR-143 and its target receptor 5-HT2B on agonistic behavior in the Chinese mitten crab (Eriocheir sinensis)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yang-Yang Pang ◽  
Gen-Yong Huang ◽  
Ya-Meng Song ◽  
Xiao- Zhe Song ◽  
Jia-Huan Lv ◽  
...  
Keyword(s):  
Agonistic Behavior ◽  
Cost Effective ◽  
Eriocheir Sinensis ◽  
Chinese Mitten Crab ◽  
Physical Damage ◽  
Phylogenetic Tree Analysis ◽  
Important Species ◽  
Downstream Target ◽  
Mitten Crab

AbstractChinese mitten crab (Eriocheir sinensis) as a commercially important species is widely cultured in China. However, E. sinensis is prone to agonistic behavior, which causes physical damage and wastes energy resources, negatively impacting their growth and survival. Therefore, understanding the regulatory mechanisms that underlie the switching of such behavior is essential for ensuring the efficient and cost-effective aquaculture of E. sinensis. The 5-HT2B receptor is a key downstream target of serotonin (5-HT), which is involved in regulating animal behavior. In this study, the full-length sequence of 5-HT2B gene was cloned. The total length of the 5-HT2B gene was found to be 3127 bp with a 236 bp 5′-UTR (untranslated region), a 779 bp 3′-UTR, and a 2112 bp open reading frame encoding 703 amino acids. Phylogenetic tree analysis revealed that the 5-HT2B amino acid sequence of E. sinensis is highly conserved with that of Cancer borealis. Using in vitro co-culture and luciferase assays, the miR-143 targets the 5-HT2B 3′-UTR and inhibits 5-HT2B expression was confirmed. Furthermore, RT-qPCR and Western blotting analyses revealed that the miR-143 mimic significantly inhibits 5-HT2B mRNA and protein expression. However, injection of miR-143 did not decrease agonistic behavior, indicating that 5-HT2B is not involved in the regulation of such behavior in E. sinensis.

A spiroplasma associated with tremor disease in the Chinese mitten crab (Eriocheir sinensis)

Microbiology ◽  
2004 ◽  
Vol 150 (9) ◽  
pp. 3035-3040 ◽  
Author(s):  
Wen Wang ◽  
Bohai Wen ◽  
Gail E. Gasparich ◽  
Ningning Zhu ◽  
Liwen Rong ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Light And Electron Microscopy ◽  
Eriocheir Sinensis ◽  
Rrna Gene ◽  
Chinese Mitten Crab ◽  
Mitten Crab ◽  
The 16S Rrna Gene

An epidemic of tremor disease has been a serious problem in Chinese mitten crabs, Eriocheir sinensis, in China in recent years. The disease-causing agent was previously considered to be a rickettsia-like organism. Here, analysis of the 16S rRNA gene sequence, light and electron microscopy and cultivation in vitro were used to identify the agent. Sequence analysis of the 16S rRNA gene found it to have 98 % identity with that of Spiroplasma mirum. The agent was able to be passed through membrane filters with pores 220 nm in diameter and could be cultivated by inoculating the yolk sac of embryonated chicken eggs and M1D medium. Rotary motion and flexional movement were seen by light microscopy, and electron microscopy showed that the organism had a helical morphology and lacked a cell wall. The organism produced small colonies with a diameter of 40–50 μm after 17–25 days of incubation on solid M1D medium. The agent was found in blood cells, muscles, nerves and connective tissues of crabs inoculated with a filtrate of yolk sacs or with cultures grown in M1D medium, and it was similar in structure to those grown in eggs and cultivation broth. Disease was reproduced by experimental infection with the cultivated organisms. This study has demonstrated that the causative agent of tremor disease in the Chinese mitten crab is a member of the genus Spiroplasma. This is believed to be the first time a spiroplasma has been found in a crustacean. These findings are not only significant for studies on pathogenic spiroplasmas, but also have implications for studies of freshwater ecology.

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