Synergistic action between fractions of essential oils from Cymbopogon citratus, Ocimum gratissimum and Thymus vulgaris against Penicillium expansum

Aims: The present investigations describes bactericidal potential of essential oil for management of Xanthomonas albilineans (Ashby. 1929) Dowson 1943, pathogen responsible for sugarcane Leaf scald disease in Côte d'Ivoire. Study Design: the study was conducted at the Ferké 1 sugar mill and the Laboratory of Plant Physiology of Felix Houphouët BOIGNY University. Methodology: Diseased leaves specimens showing signs of X. albilineans presence were collected from sugarcane industrial plantations in Côte d'Ivoire. Pathogenicity was confirmed by observing Koch’s postulates under semi-controlled conditions. Asymptomatic leaves, obtained after 60 days of culture, were inoculated at three points by infiltration with bacterial suspension calibrated at 108 bacteria/ml. Apparition of small tan-brown necrotic lesions on the leaf blade, parallel to the veins and prolonged to the ends by a discolored vascular bundle confirmed that disease is caused by Xanthomonas albilineans. Three essential oils of aromatic plants whose antibacterial properties are recognized were used at 100; 300; 500; 1000 and 2000 ppm in comparison with Callicuivre (56% copper oxychloride) used as reference product. Results: The aqueous solutions based on these essential oils induced an inhibition zone of bacterial growth proportional to the dose applied and the incubation period. Thus, on day 5 of incubation, the essential oil solution of Ocimum gratissimum L. at 100 ppm induced an average inhibition zone diameter not significantly different from the one induced by the Cymbopogon citratus (DC.) Stapf solution at 300 ppm and the one of copper oxychloride at 1000 ppm. At 1000 ppm, Zingiber officinale Roscoe, Cymbopogon citratus (DC.) Stapf and Ocimum gratissimum L essential oils induced equivalent inhibition rates (6 mm) that were higher than that of the control (3.5 mm). Conclusion: sugarcane varieties cultivated at the Ferké 1 sugar mill complex, despite the selection for resistance are threatened by Leaf Scald Disease, which is significantly expressed on variety R585. Cymbopogon citratus (DC.) Stapf and Ocimum gratissimum L oils have the strongest antibacterial activity and may be useful to control Leaf Scald Disease.

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GC/MS Profiling and Insecticidal Potential of Essential Oils from Thymus vulgaris (Lamiaceae) and Cymbopogon citratus (Poaceae) against tomato borer, Tuta absoluta

10.20944/preprints201909.0121.v1 ◽

2019 ◽

Cited By ~ 1

Author(s):

Marie Danièle T. Ngongang ◽

Pierre Eke ◽

Modeste Lambert Sameza ◽

Champlain Lordon Djiéto ◽

Fabrice Fekam Boyom

Keyword(s):

Essential Oils ◽

Lethal Dose ◽

Field Trials ◽

Tuta Absoluta ◽

Thymus Vulgaris ◽

Cymbopogon Citratus ◽

Alpha Pinene ◽

Lemongrass Oil ◽

Recorded Data ◽

Tomato Borer

This study aimed to determine the Gas Chrommatography (GC)-Mass Spectrometry (MS) profiles and insecticidal activity of essential oils (EOs) from Thymus vulgaris (Thyme) and Cymbopogon citratus (Lemongrass) against the invasive and devastating pest, Tuta absoluta (T. absoluta) through contact and fumigation routes. We found out that thyme oil was predominantly constituted of Thymol (22.16%), α-Pinene (15.35%) and p-Cymene (13.54%) whilst Neral (21.41%), Geranial (21.36%) and β-Myrcene (9.74%) were the major constituents of lemongrass oil. Lemongrass oil exhibited higher insecticidal efficiency irrespective of application mode with 50% lethal dose (LD50) values of 35.8 and 72.2 µL.L-1air on contact and fumigation routes, respectively. Lemongrass oil also lengthened pupal duration at all tested doses irrespective of application routes. The overall responses of Lemongrass oil surpassed that of the reference insecticide (Lynx®: Lambda-cyhalothrine; Acetamipride). Thus, the recorded data clearly showed the acute and long-term insecticidal effects of the studied EOs, though a greenhouse and open field trials are required prior to the validation of this approach as remediation measure for Integrated Pest Management (IPM) for tomato borer control in Cameroon and elsewhere.

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Chemical constituents of essential oils from Thymus vulgaris and Cymbopogon citratus and their insecticidal potential against the tomato borer, Tuta absoluta (Lepidoptera: Gelechiidae)

International Journal of Tropical Insect Science ◽

10.1007/s42690-021-00514-7 ◽

2021 ◽

Author(s):

Marie Danièle Tchonang Ngongang ◽

Pierre Eke ◽

Modeste Lambert Sameza ◽

Madeleine Nina Love Ngo Mback ◽

Champlain Djiéto- Lordon ◽

...

Keyword(s):

Essential Oils ◽

Chemical Constituents ◽

Tuta Absoluta ◽

Thymus Vulgaris ◽

Cymbopogon Citratus ◽

Tomato Borer

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Effect of essential oils on cattle gastrointestinal nematodes assessed by egg hatch, larval migration and mortality testing

Journal of Helminthology ◽

10.1017/s0022149x19001081 ◽

2019 ◽

Vol 94 ◽

Author(s):

S. Saha ◽

S. Lachance

Keyword(s):

Essential Oils ◽

Anthelmintic Activity ◽

Gastrointestinal Nematodes ◽

Mentha Piperita ◽

Tween 20 ◽

Thymus Vulgaris ◽

Cymbopogon Citratus ◽

Egg Hatching ◽

Egg Hatch ◽

Larval Migration

Abstract The efficacy of eight essential oils (EOs) (Solidago canadensis, Eucalyptus globulus, Pelargonium asperum, Ocimum basilicum, Thymus vulgaris, Mentha piperita, Cymbopogon citratus and Cymbopogon martinii) against gastrointestinal nematodes (GINs) was evaluated using eggs collected from naturally infected cattle and cultured infective larvae (L3). The larvae species cultured from the faecal samples and subjected to two in vitro tests were Haemonchus spp. (55.5%), Trichostrongylus spp. (28.0%), Cooperia spp. (15.0%) and Oesophagostomum spp. (1.5%). The genus of EO Cymbopogon (C. citratus and C. martinii) showed the highest anthelmintic activity at the dose of 8.75 mg/ml, for the egg hatch, the larval migration and mortality assays. All of the EOs tested reduced egg hatching to rates <19.0%, compared to the controls (water and water + Tween 20) that had rates >92.0%. Cymbopogon citratus and C. martinii treatments resulted in 11.6 and 8.1% egg hatch, had the lowest migration of larvae through sieves, 60.5 and 54.9%, and the highest mortality rates, 63.3 and 56.3%, respectively. Dose–response tests showed that EO from C. citratus had the lowest larval LC50 and migration inhibition concentration (IC50) values of 3.89 and 7.19 mg/ml, respectively, compared to two other EOs (C. martinii and O. basilicum). The results suggest that EOs from the genus Cymbopogon can be interesting candidates for nematode control in cattle, although it may prove challenging to deliver concentrations to the gastrointestinal tract sufficient to effectively manage GINs.

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