Chemical constituents of essential oils from Thymus vulgaris and Cymbopogon citratus and their insecticidal potential against the tomato borer, Tuta absoluta (Lepidoptera: Gelechiidae)

Author(s):  
Marie Danièle Tchonang Ngongang ◽  
Pierre Eke ◽  
Modeste Lambert Sameza ◽  
Madeleine Nina Love Ngo Mback ◽  
Champlain Djiéto- Lordon ◽  
...  
Author(s):  
Marie Danièle T. Ngongang ◽  
Pierre Eke ◽  
Modeste Lambert Sameza ◽  
Champlain Lordon Djiéto ◽  
Fabrice Fekam Boyom

This study aimed to determine the Gas Chrommatography (GC)-Mass Spectrometry (MS) profiles and insecticidal activity of essential oils (EOs) from Thymus vulgaris (Thyme) and Cymbopogon citratus (Lemongrass) against the invasive and devastating pest, Tuta absoluta (T. absoluta) through contact and fumigation routes. We found out that thyme oil was predominantly constituted of Thymol (22.16%), α-Pinene (15.35%) and p-Cymene (13.54%) whilst Neral (21.41%), Geranial (21.36%) and β-Myrcene (9.74%) were the major constituents of lemongrass oil. Lemongrass oil exhibited higher insecticidal efficiency irrespective of application mode with 50% lethal dose (LD50) values of 35.8 and 72.2 µL.L-1air on contact and fumigation routes, respectively. Lemongrass oil also lengthened pupal duration at all tested doses irrespective of application routes. The overall responses of Lemongrass oil surpassed that of the reference insecticide (Lynx®: Lambda-cyhalothrine; Acetamipride). Thus, the recorded data clearly showed the acute and long-term insecticidal effects of the studied EOs, though a greenhouse and open field trials are required prior to the validation of this approach as remediation measure for Integrated Pest Management (IPM) for tomato borer control in Cameroon and elsewhere.


Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
EG Nkouaya Mbanjo ◽  
F Tchoumbougnang ◽  
PM Jazet Dongmo ◽  
ML Sameza ◽  
PH Amvam Zollo ◽  
...  

2019 ◽  
Vol 94 ◽  
Author(s):  
S. Saha ◽  
S. Lachance

Abstract The efficacy of eight essential oils (EOs) (Solidago canadensis, Eucalyptus globulus, Pelargonium asperum, Ocimum basilicum, Thymus vulgaris, Mentha piperita, Cymbopogon citratus and Cymbopogon martinii) against gastrointestinal nematodes (GINs) was evaluated using eggs collected from naturally infected cattle and cultured infective larvae (L3). The larvae species cultured from the faecal samples and subjected to two in vitro tests were Haemonchus spp. (55.5%), Trichostrongylus spp. (28.0%), Cooperia spp. (15.0%) and Oesophagostomum spp. (1.5%). The genus of EO Cymbopogon (C. citratus and C. martinii) showed the highest anthelmintic activity at the dose of 8.75 mg/ml, for the egg hatch, the larval migration and mortality assays. All of the EOs tested reduced egg hatching to rates <19.0%, compared to the controls (water and water + Tween 20) that had rates >92.0%. Cymbopogon citratus and C. martinii treatments resulted in 11.6 and 8.1% egg hatch, had the lowest migration of larvae through sieves, 60.5 and 54.9%, and the highest mortality rates, 63.3 and 56.3%, respectively. Dose–response tests showed that EO from C. citratus had the lowest larval LC50 and migration inhibition concentration (IC50) values of 3.89 and 7.19 mg/ml, respectively, compared to two other EOs (C. martinii and O. basilicum). The results suggest that EOs from the genus Cymbopogon can be interesting candidates for nematode control in cattle, although it may prove challenging to deliver concentrations to the gastrointestinal tract sufficient to effectively manage GINs.


2018 ◽  
Vol 15 (2) ◽  
pp. e1700436 ◽  
Author(s):  
Giordana Feriotto ◽  
Nicola Marchetti ◽  
Valentina Costa ◽  
Simone Beninati ◽  
Federico Tagliati ◽  
...  

2021 ◽  
Vol 42 (6supl2) ◽  
pp. 3837-3854
Author(s):  
Geraldo Márcio Costa ◽  
◽  
Ingrid Alexandre Prado ◽  
Glei dos Anjos de Carvalho-Castro ◽  
Glaúcia Frasnelli Mian ◽  
...  

Canine otitis externa is a disease that affects the external acoustic meatus of about 20% of dogs at some point in life, without predilection for race, age, or sex. It is a multifactorial disease whose etiology involves several microorganisms, detaching Staphylococcus aureus. Antimicrobials are the basis for treating this illness; however, due to the increase in antimicrobial resistance, conventional drugs have become ineffective, requiring the search for alternative therapies. In this context, essential oils (EOs) have great therapeutic potential due to their broad antimicrobial action. This study aimed to evaluate the minimum inhibitory concentration (MIC) in order to measure the MIC50 and MIC90 of gentamicin and EOs of Eugenia caryophyllata, Thymus vulgaris, Cymbopogon winterianus, Cymbopogon citratus, and Cinnamomum cassia against 62 Staphylococcus aureus strains isolated from the external acoustic meatus of dogs. All EOs showed antibacterial action against the studied microorganisms, and their MIC50 and MIC90 were as follows: Eugenia caryophyllata, 2.42 mg mL-1 and 7.25 mg mL-1; Thymus vulgaris, 9.51 mg mL-1 and 22.94 mg mL-1; Cymbopogon winterianus, 26.78 mg mL-1 and 157.79 mg mL-1; Cinnamomum cassia and Cymbopogon citratus, lower than 16.48 and 27.81 mg mL-1, with the same MIC for all isolates. The MIC50 and MIC90 found for gentamicin were 1μg mL-1 and 8 μg mL-1. The MIC range found to antibiotic in this assay was 0.5 μg mL-1 to 128 μg mL-1, and the isolates were classified as susceptible [48 strains (77.41%) - MIC range of 0.5-4.0 μg mL-1], intermediate [eight strains (12.90%) - (MIC = 8.0 μg mL-1], or resistant [six strains (9.68%) - MIC ≥ 16 μg mL-1]. The results, according to the in vitro assays, showed that resistance to gentamicin, one of the antimicrobials most commonly used to treat canine otitis, is present in the Staphylococcus aureus population evaluated. Additionally, the tested EOs have great potential for therapeutic use, however future studies should be carried out to evaluate their in vivo efficacy.


2016 ◽  
Vol 2016 ◽  
pp. 1-4 ◽  
Author(s):  
Valéria Maria Lara ◽  
Adriano Bonfim Carregaro ◽  
Deise Flores Santurio ◽  
Mariangela Facco de Sá ◽  
Janio Moraes Santurio ◽  
...  

This study evaluated thein vitroantibacterial activity of essential oils fromLippia graveolens(Mexican oregano),Origanum vulgaris(oregano),Thymus vulgaris(thyme),Rosmarinus officinalis(rosemary),Cymbopogon nardus(citronella),Cymbopogon citratus(lemongrass), andEucalyptus citriodora(eucalyptus) againstEscherichia coli(n=22) strains isolated fromAlouattaspp. feces. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined for each isolate using the broth microdilution technique. Essential oils of Mexican oregano (MIC mean = 1818 μg mL−1; MBC mean = 2618 μg mL−1), thyme (MIC mean = 2618 μg mL−1; MBC mean = 2909 μg mL−1), and oregano (MIC mean = 3418 μg mL−1; MBC mean = 4800 μg mL−1) showed the best antibacterial activity, while essential oils of eucalyptus, rosemary, citronella, and lemongrass displayed no antibacterial activity at concentrations greater than or equal to 6400 μg mL−1. Our results confirm the antimicrobial potential of some essential oils, which deserve further research.


2012 ◽  
Vol 32 (1) ◽  
pp. 167-172 ◽  
Author(s):  
Alessandra Farias Millezi ◽  
Danila Soares Caixeta ◽  
Diogo Francisco Rossoni ◽  
Maria das Graças Cardoso ◽  
Roberta Hilsdorf Piccoli

Several essential oils of condiment and medicinal plants possess proven antimicrobial activity and are of important interest for the food industry. Therefore, the Minimum Inhibitory Concentrations (MIC) of those oils should be determined for various bacteria. MIC varies according to the oil used, the major compounds, and the physiology of the bacterium under study. In the present study, the essential oils of the plants Thymus vulgaris (time), Cymbopogon citratus (lemongrass) and Laurus nobilis (bay) were chemically quantified, and the MIC was determined on the bacteria Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Listeria monocytogenes ATCC 19117, Salmonella enterica Enteritidis S64, and Pseudomonas aeruginosa ATCC 27853. The essential oil of C. citratus demonstrated bacterial activity at all concentrations tested and against all of the bacteria tested. The majority of essential oil compounds were geranial and neral. The major constituent of T. vulgaris was 1.8-cineol and of L. nobilis was linalool, which presented lower antibacterial activity, followed by 1.8-cineol. The Gram-negative bacteria demonstrated higher resistance to the use of the essential oils tested in this study. E. coli was the least sensitive and was inhibited only by the oils of C. citratus and L. nobilis.


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