The emulsification properties of alkaline-extracted polysaccharide conjugates from Apocynum venetum L. tea residues

Apocynum venetum L. (A. venetum) has long been used in oriental folk medicine for the treatment of some liver diseases; however, the underlying mechanisms remain to be fully elucidated. Acetaminphen (APAP) is a widely used analgesic drug that can cause acute liver injury in overdose situations. In this study, we investigated the potential protective effect of A. venetum leaf extract (ALE) against APAP-induced hepatotoxicity. Mice were intragastrically administered with ALE once daily for 3 consecutive days prior to receiving a single intraperitoneal injection of APAP. The APAP group showed severe liver injury characterized by the noticeable fluctuations in the following parameters: serum aminotransferases; hepatic malondialdehyde (MDA), 3-nitrotyrosine (3-NT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione (GSH). These liver damages induced by APAP were significantly attenuated by ALE pretreatments. A collective analysis of histopathological examination, DNA laddering and western blot for caspase-3 and cytochrome c indicated that the ALE is also capable of preventing APAP-induced hepatocyte death. Hyperoside, isoquercitrin and their derivatives have been identified as the major components of ALE using HPLC-MS/MS. Taken together, the A. venetum possesses hepatoprotective effects partially due to its anti-oxidant action.

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Protective effect of the total flavonoids from Apocynum venetum L. on carbon tetrachloride-induced hepatotoxicity in vitro and in vivo

Journal of Physiology and Biochemistry ◽

10.1007/s13105-018-0618-0 ◽

2018 ◽

Vol 74 (2) ◽

pp. 301-312 ◽

Cited By ~ 13

Author(s):

Wei Zhang ◽

Zheng Dong ◽

Xiujuan Chang ◽

Cuihong Zhang ◽

Guanghua Rong ◽

...

Keyword(s):

Carbon Tetrachloride ◽

Protective Effect ◽

Total Flavonoids ◽

Apocynum Venetum

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Genomic Survey, Transcriptome, and Metabolome Analysis of Apocynum venetum and Apocynum hendersonii to Reveal Major Flavonoid Biosynthesis Pathways

Metabolites ◽

10.3390/metabo9120296 ◽

2019 ◽

Vol 9 (12) ◽

pp. 296 ◽

Cited By ~ 5

Author(s):

Gang Gao ◽

Ping Chen ◽

Jikang Chen ◽

Kunmei Chen ◽

Xiaofei Wang ◽

...

Keyword(s):

Morphological Difference ◽

Flavonoid Biosynthesis ◽

Flavonoid Content ◽

Metabolome Analysis ◽

Total Anthocyanin ◽

Small Genome ◽

Genome Survey ◽

Metabolite Profiles ◽

Apocynum Venetum ◽

Gene Expression Levels

Apocynum plants, especially A. venetum and A. hendersonii, are rich in flavonoids. In the present study, a whole genome survey of the two species was initially carried out to optimize the flavonoid biosynthesis-correlated gene mining. Then, the metabolome and transcriptome analyses were combined to elucidate the flavonoid biosynthesis pathways. Both species have small genome sizes of 232.80 Mb (A. venetum) and 233.74 Mb (A. hendersonii) and showed similar metabolite profiles with flavonols being the main differentiated flavonoids between the two specie. Positive correlation of gene expression levels (flavonone-3 hydroxylase, anthocyanidin reductase, and flavonoid 3-O-glucosyltransferase) and total flavonoid content were observed. The contents of quercitrin, hyperoside, and total anthocyanin in A. venetum were found to be much higher than in A. hendersonii, and such was thought to be the reason for the morphological difference in color of A. venetum and A. hendersonii. This study provides valuable genomic and metabolome information for understanding of A. venetum and A. hendersonii, and lays a foundation for elucidating Apocynum genus plant flavonoid biosynthesis.

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Physıologıcal response of Apocynum venetum L. seedlıngs under osmotıc stress

Bangladesh Journal of Botany ◽

10.3329/bjb.v44i4.38569 ◽

2018 ◽

Vol 44 (4) ◽

pp. 551-556

Author(s):

Hua Zhao ◽

Huiping Dai

Keyword(s):

Superoxide Dismutase ◽

Polyethylene Glycol ◽

Drought Stress ◽

Physiological Response ◽

Antioxidant Defense ◽

Soluble Sugar ◽

Defense Systems ◽

Apocynum Venetum ◽

Antioxidant Defense Systems ◽

Effects Of Drought

Effects of drought stress were induced by polyethylene glycol (PEG-6000) (10, 20, and 30% ) for 2, 4, 6 and 8 days in Apocynum venetum L. seedlings with the potting method. The results showed that PEG significantly increased the activities of glutathione reductase (GR), superoxide dismutase (SOD), ascorbate peroxidase (APX), and the contents of electrolyte leakage rates, MDA, proline and soluble sugar were increased from 30% PEG. Thus, it was indicated that the application of exogenous 30% PEG induced oxidative damage by enhancing antioxidant defense systems.

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Antidepressant Effects of Apocynum venetum Leaves in a Forced Swimming Test.

Biological and Pharmaceutical Bulletin ◽

10.1248/bpb.24.848 ◽

2001 ◽

Vol 24 (7) ◽

pp. 848-851 ◽

Cited By ~ 63

Author(s):

Veronika BUTTERWECK ◽

Sansei NISHIBE ◽

Tsutomu SASAKI ◽

Masaru UCHIDA

Keyword(s):

Forced Swimming Test ◽

Forced Swimming ◽

Antidepressant Effects ◽

Apocynum Venetum ◽

Swimming Test

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A Study on the Antibacterial Property of the Extract from Apocynum Venetum

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.332-334.164 ◽

2011 ◽

Vol 332-334 ◽

pp. 164-167

Author(s):

Zheng Li ◽

Zhen Feng Dong ◽

Jian Fei Zhang ◽

Ji Xian Gong ◽

Fan Jie Meng

Keyword(s):

Escherichia Coli ◽

Saccharomyces Cerevisiae ◽

Staphylococcus Aureus ◽

Aspergillus Niger ◽

Antibacterial Effect ◽

Antibacterial Property ◽

Absolute Ethanol ◽

Antibacterial Substance ◽

Apocynum Venetum ◽

Better Than

In order to determined the antibacterial property of Apocynum venetum. Three kinds of extractants (distilled water, 75% ethanol and absolute ethanol) were used to extract the leaf, stick and skin of Apocynum venetum, respectively. Flask and plate methods were used to evaluate the antibacterial property of these extracts on four kinds of classical microbiologies, Escherichia coli, Staphylococcus aureus, Saccharomyces cerevisiae and Aspergillus niger, respectively. The results showed that the antibacterial rate of extract by 75 % ethanol was better than extracts by water or absolute ethanol on Escherichia coli and skin contained more antibacterial substance (on Escherichia coli) than others. The antibacterial rate of extract by distill water was better than extracts by 75 % ethanol or absolute ethanol on Staphylococcus aureus and stick contained more antibacterial substance (on Staphylococcus aureus) than others. Some of extracts had very weak antibacterial rate on Saccharomyces cerevisiae and all extracts had not antibacterial effect on Aspergillus niger.

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