Amino acid composition, mineral contents and protein solubility of some lima bean (Phaseolus lunatus l. Walp) seeds coat

2015 ◽  
Vol 73 ◽  
pp. 130-134 ◽  
Author(s):  
Kudirat Titilope Seidu ◽  
Oluwatooyin Faramade Osundahunsi ◽  
Mary Tolulope Olaleye ◽  
Isaac Babatunde Oluwalana
2020 ◽  
Vol 16 (2) ◽  
pp. 60
Author(s):  
Nwozo Sarah Onyenibe ◽  
Julius Oluwaseun Oluwafunmilola ◽  
Stanley Udogadi Nwawuba

The extracted seeds of African breadfruit are identified to be extremely healthy whenever it is correctly processed. Therefore, the aim of the present study was to evaluate the effects of processing methods on the nutritional quality of African breadfruit seed. A qualitative phytochemical analysis including: Alkaloid, Flavonoid, Saponin, Tannin, Anthraquinone, Terpenoids, Steroid, and Cardiac Glycosides for the different fraction of African breadfruit seed was performed using a standard method. The result revealed the presence and greater amount of phytochemical for the raw fraction; seven in eight, six in eight for steamed fraction, and four in eight for boiled and roasted respectively. Anti-nutrient, Proximate, and Mineral Content were also conducted using standard methods. The amino acid composition was determined using High-Performance Liquid Chromatography (HPLC). The results of the present study revealed that anti-nutrients including Phytate, Tannins, and Oxalate were significantly p<0.05 reduced in the boiled fraction 5.47±0.15, 3.42±0.02 and 6.89±0.05, and highest in the raw fraction 7.77±0.01, 5.09±0.03 and 9.34±0.14. The proximate composition including; percentage crude fat, Ash, Carbohydrate, Fatty acid, and Energy value were significantly lower p<0.05 in the boiled fraction relative to the other fractions. Mineral contents; calcium, magnesium, sodium, potassium, and phosphorus were also significantly p<0.05 elevated in the boiled fraction relative to the raw, steamed, and roasted fraction. The amino acid composition was highest in the roasted and boiled fraction 57.350 and 56.978, and lowest in the steamed and raw fraction 35.754 and 28.748 respectively. Therefore, boiling (cooking) is encouraged for the preparation of African breadfruit seed.


RSC Advances ◽  
2015 ◽  
Vol 5 (81) ◽  
pp. 66451-66463 ◽  
Author(s):  
T. M. Hicks ◽  
C. J. R. Verbeek ◽  
M. C. Lay ◽  
M. Manley-Harris

The effect of oxidative decolouring with peracetic acid on the physical and chemical characteristics of bloodmeal proteins was investigated by assessing protein solubility, molecular weight distribution and final amino acid composition.


2021 ◽  
pp. 40-47
Author(s):  
Adunola Abosede Bello ◽  
Oluwafunmike Blessing Adepoju ◽  
Oluwasola Toluwalope Moradeyo ◽  
Olayinka Josephine Okuneye ◽  
Oluwafemi Akinsola Omole

The biochemical composition of three cultured cichlids (Tilapia zilli, Tilapia guineensis and Orechromis aureus) were evaluated and compared.  The proximate composition of the cichlids was determined using official methods of analysis, mineral composition was determined using Atomic Absorption Spectrophotometer and the amino acid composition was analyzed using Amino Acid Analyzer. The proximate composition of the three cultured species of tilapia fish (T. zilli, T. guineensis and O. aureus) indicated that moisture content, crude fat, crude fiber and ash content showed significant difference (p<0.05) among the three species while crude protein and carbohydrate content showed no significant difference (p<0.05) among the three species. The mineral contents such as zinc, magnesium and manganese showed significant difference (p<0.05) among the three species of tilapia (T. zilli, T. guineensis and O. aureus) while sodium, potassium, calcium, iron, phosphorus and copper contents showed no significant difference (p<0.05) among the three species. The amino acid composition showed lysine as the most abundant amino acids present in all the cultured cichlids studied. This shows that these cultured cichlids are highly nutritious and would be of great value to consumers.


1979 ◽  
Vol 6 (1) ◽  
pp. 25 ◽  
Author(s):  
PA Gleeson ◽  
MA Jermyn

Variation in the conditions of extraction of β-lectins from a number of seeds and plant tissues resulted in differences in the amino acid composition and the protein content of the isolated product. These differences may be due to differential extraction of the β-lectin, degradative losses of protein or carbohydrate components of the β-lectin, or a combination of these possibilities. Subtilisin treatment of the Alocasia macrorrhizos β-lectin removes 80% of the protein and the material remaining after enzymic treatment retains the capacity to bind to the Yariv artificial antigen. A number of β-lectins were treated with subtilisin and the amino acid compositions of the residual material were similar, with high contents of hydroxyproline, alanine and serine. The amino acid composition of the Phaseolus lunatus β-lectin was not altered by β-elimination. The capacity of the β-lectin to bind to the Yariv artificial antigens was eliminated by progressive acid hydrolysis under conditions where both glycosidic and peptide linkages may be labile.


2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Sule O. Salawu ◽  
Oluwaseun M. Folorunso ◽  
Akintunde A. Akindahunsi ◽  
Aline A. Boligon

The present investigation was designed to characterize the phenolic profile of Lima beans (Phaseolus Lunatus) and also to evaluate the antioxidant indices: total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP), 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and amino acid composition at different stages of simulated gastrointestinal digestion (oral, gastric, intestinal). High Performance Liquid Chromatography (HPLC-DAD) analysis revealed the presence of some phenolic compounds (gallic acid, catechin, caffeic acid, rutin, quercitrin, quercetin, kaempferol and apigenin), with a reduced amount (mg/g) after cooking; gallic acid (raw: 1.96 ± 0.02; cooked: 1.82 ± 0.01); catechin (raw: 0.83 ± 0.01; cooked: 0.73 ± 0.01); rutin (raw: 2.61 ± 0.03; cooked:1.74 ±0.03); quercitrin (raw: 5.73 ± 0.01; cooked: 5.68 ± 0.01); apigenin (raw: 2.09 ±0.01; cooked:1.79 ± 0.02),  with exception of quercetin (raw: 2.11 ±0.02; cooked: 5.73 ±  0.02) and caffeic acid (raw: 2.08 ±0.04; cooked: 2.95 ± 0.04). The results of antioxidant indices of in vitro enzyme digested lima beans revealed higher values for cooked Lima beans compared to the raw counterpart, with a stepwise increase at the different stages of in vitro digestion, with the exception of ferric reducing antioxidant power; TPC (oral digestion: 65.44 ± 0.96; gastric digestion:134.87± 0.46; intestinal digestion:517.72 ± 4.70; mg/g tannic acid equivalent), TFC (oral digestion: 199.30 ± 6.43; gastric digestion: 1065.97 ± 1.22; intestinal digestion: 3691.87 ± 4.2; mg/g quercetin equivalent), DPPH (oral digestion: 85800.00 ± 305.50; gastric digestion: 99066.66 ± 115.47; intestinal digestion: 211354.20 ± 360.84 µmol TE/g sample). The results also revealed a progressive increase in the antioxidant indices and amino acid composition (mg/kg) for both raw and processed lima beans at various stages of the in vitro digestion, with the intestinal phase of simulated digestion ranking higher. This implied that the Lima beans contained some essential amino acids and antioxidant molecules that would be readily available after passing through the gastrointestinal tract and could therefore be explored as functional food in the management of free radical mediated diseases.


2010 ◽  
Vol 17 (12) ◽  
pp. 1466-1472 ◽  
Author(s):  
Niu Xiaohui ◽  
Li Nana ◽  
Shi Feng ◽  
Hu Xuehai ◽  
Xia Jingbo ◽  
...  

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