Death by electrocution: Histological technique for copper detection on the electric mark

2016 ◽  
Vol 264 ◽  
pp. 24-27 ◽  
Author(s):  
Enrico Bellini ◽  
Gary Gambassi ◽  
Giulia Nucci ◽  
Matteo Benvenuti ◽  
Gianluca Landi ◽  
...  
2005 ◽  
Vol 108-109 ◽  
pp. 643-648 ◽  
Author(s):  
Marko Yli-Koski ◽  
Hele Savin ◽  
E. Saarnilehto ◽  
Antti Haarahiltunen ◽  
Juha Sinkkonen ◽  
...  

We compare SPV technique with µ−PCD for the determination of recombination activity of copper precipitates in p-Si. The copper precipitates were formed in bulk silicon through illumination at room temperature. We observed that the recombination activities of copper precipitates measured with SPV are higher than the ones measured with µ−PCD technique. However, it seems that the copper detection sensitivity is about the same with SPV and µ−PCD techniques.


Although, as is well known, the ultra-microscopic viruses are invisible in histological preparations, yet characteristic bodies occur within certain of the cells of animals infected with such organisms. The origin and nature of these so-called “virus bodies” has been the subject of much controversy. By some they have been regarded as the actual parasite, or at least as phases in its life cycle. To von Prowazek they were dual in character consisting of microorganisms embedded in material produced by the reaction of the cytoplasm of the infected cell. Still other observers regarded such bodies as products of cellular disintegration. In a former paper (Findlay and Ludford (1926)) we have referred to the various views held by writers in this field and have made a survey of the literature of the subject in the form of a pictographic review. We shall, therefore, only mention previous work in this field, in so far as it directly concerns our personal observations. A conspicuous fault of much of the earlier work has been the unsatisfactory histological technique employed, especially the nature of the fixative. The earlier cytological work was carried out with fixatives, which although satisfactory for the subsequent demonstration of nuclear structures were very destructive to the cytoplasm. This is particularly unfortunate since most of the virus bodies occur in the ground cytoplasm of cells. We have endeavoured to rectify this source of error in our work by employing fixatives, which have been proved to fix the cells in such a manner as to give an appearance as nearly as possible identical with their structure, as seen in the living cells.


2013 ◽  
Vol 49 (11) ◽  
pp. 1103 ◽  
Author(s):  
Alfonso Salinas-Castillo ◽  
Maria Ariza-Avidad ◽  
Christian Pritz ◽  
Maria Camprubí-Robles ◽  
Belen Fernández ◽  
...  

2018 ◽  
Vol 52 (6) ◽  
pp. 3567-3573 ◽  
Author(s):  
Casey W. Quinn ◽  
David M. Cate ◽  
Daniel D. Miller-Lionberg ◽  
Thomas Reilly ◽  
John Volckens ◽  
...  

Sensors ◽  
2019 ◽  
Vol 19 (20) ◽  
pp. 4514 ◽  
Author(s):  
Nour Doumani ◽  
Elias Bou-Maroun ◽  
Jacqueline Maalouly ◽  
Maya Tueni ◽  
Adrien Dubois ◽  
...  

For efficiently measuring copper (II) ions in the acidic media of white wine, a new chemosensor based on rhodamine B coupled to a tetraazamacrocyclic ring (13aneN4CH2NH2) was designed and synthesized by a one-pot reaction using ethanol as a green solvent. The obtained chemosensor was characterized via NMR, UV and fluorescent spectra. It was marked with no color emission under neutral pH conditions, with a pink color emission under acidic conditions, and a magenta color emission under acidic conditions where copper (II) ions were present. The sensitivity towards copper (II) ions was tested and verified over Ca2+, Ag+, Zn2+, Mg2+, Co2+, Ni2+, Fe2+, Pb2+, Cd2+, Fe3+, and Mn2+, with a detection limit of 4.38 × 10−8 M in the fluorescence spectrum.


1985 ◽  
Vol 22 (4) ◽  
pp. 327-332 ◽  
Author(s):  
L. P. Thornburg ◽  
M. Beissenherz ◽  
M. Dolan ◽  
M. F. Raisbeck

Three different histochemical methods for copper detection were compared. Atomic absorption analysis was used to substantiate the tissue stains. There was good correlation between rhodanine staining and rubeanic acid-stained tissue sections. The orcein reaction for copper-associated protein did not consistently correlate with the methods demonstrating copper. Prolonged staining (72 hours) with rubeanic acid more consistently and clearly detected increased copper in canine livers than did staining with rhodanine. Seventy-two hour staining with rubeanic acid is the method of choice for histochemical detection of copper in canine liver.


Author(s):  
S.R. Flynn ◽  
B.S. Nakashima ◽  
M.P.M. Burton

Assessment of spawning survival for female capelin Mallotus villosus (Pisces: Osmeriformes) greater than 12 cm in length was made in the autumn of 1995–1997 comparing a histological technique with existing methods. An intensive macroscopic visual assessment of selected samples from 1995, 1996 and 1997 revealed that 10, 20 and 40% of the females were recovering from spawning respectively each year. Histological assessments of the same fish revealed that 20% in 1995, and nearly 60% of the fish in 1996 and 1997 showed physiological evidence of previous spawning. The proportion of recovering spawners increased with both length and age of the fish. One sample from the Northwest Atlantic Fisheries Organization area of 2J during October 1997 contained nearly 90% recovering fish. The potential impact of the fishery in light of the variability in post-spawning survival of the iteroparous female capelin is discussed.


The Analyst ◽  
2020 ◽  
Vol 145 (14) ◽  
pp. 4950-4956
Author(s):  
ReJeana Cary ◽  
Sarah Unser ◽  
Ilaina Monroe ◽  
Joseph Holbrook ◽  
Laura Sagle

A simple, point-of-care plasmonic assay for copper detection in human plasma is developed.


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