Rosa canina L. is a rich fruit on bioactive substances which provides them with high antioxidant potential, like phenolic acids, flavonoids, condensed tannins. The aim of this study was to estimate and characterize the phenolic content, flavonoids content, condensed tannins content obtained from rose hips fruits in five extracts (water, 50% v/v ethanol, 70% v/v ethanol, 80% v/v methanol, apple vinegar) to examine their antioxidant activity by two reliable methods: Total antioxidant activity and DPPH. Surprisingly, in our results, the highest polyphenol, flavonoids content and condensed tannins was observed in vinegar extract 55,51±1,16 mg GAE/g dw, 22,15±0,45 mg QE/g dw, 12,37±0,16 mg AAE/g dw respectively. Furthermore, the vinegar extract was the most effective to chelating the radical DPPH (IC50% = 0,336±0,005µg), and have the highest total antioxidant activity (7,88±0,19mg AAE/g dw). The current research showed that the vinegar was more effective than the methanol, ethanol, water for polyphenols, flavonoids, tannins extraction.