scholarly journals The sweet potato RbcS gene (IbRbcS1) promoter confers high-level and green tissue-specific expression of the GUS reporter gene in transgenic Arabidopsis

Gene ◽  
2015 ◽  
Vol 567 (2) ◽  
pp. 244-250 ◽  
Author(s):  
Noriaki Tanabe ◽  
Masahiro Tamoi ◽  
Shigeru Shigeoka
Keyword(s):  
Sweet Potato ◽  
Reporter Gene ◽  
Transgenic Arabidopsis ◽  
Specific Expression ◽  
Gus Reporter Gene ◽  
Tissue Specific Expression ◽  
Gus Reporter ◽  
Green Tissue ◽  
Rbcs Gene ◽  
High Level

scholarly journals Ectopic Expression of a Fagopyrum esculentum APETALA1 Ortholog only Rescues Sepal Development in Arabidopsis ap1 Mutant

2019 ◽  
Vol 20 (8) ◽  
pp. 2021 ◽  
Author(s):  
Zhixiong Liu ◽  
Yue Fei ◽  
Kebing Zhang ◽  
Zhengwu Fang
Keyword(s):  
Reporter Gene ◽  
Ectopic Expression ◽  
Fagopyrum Esculentum ◽  
Early Flowering ◽  
Gus Reporter Gene ◽  
Floral Organs ◽  
Gus Reporter ◽  
Core Eudicots ◽  
Petal Development ◽  
High Level

Fagopyrum esculentum (Polygonaceae: Caryophyllales) exhibits an undifferentiated perianth comprising five showy tepals, which does not completely correspond to the perianth differentiated into typical sepals and petals in most core eudicots. In Arabidopsis, the APETALA1 (AP1) gene is involved in specifying sepals and petals development. Here we isolated AP1 ortholog, FaesAP1, and a 2.2kb FaesAP1 promoter (pFaesAP1) from F. esculentum. FaesAP1 expression is mainly detectable in all floral organs and maintains at a high level when tepals elongate rapidly both in pin and thrum flowers. Moreover, the GUS reporter gene driven by pFaesAP1 was activated in flowers where the sepals were intense, but the petals very weak or absent. Additionally, FaesAP1 ectopic expression in Arabidopsis ap1-10 mutant rescues sepal development fully, obviously prompting early flowering, but failing to complement petal development. In this study, evidence was provided that the showy tepals in the F. esculentum are homologs to core eudicots sepals. Furthermore, these findings show a different perianth identity program in Caryophyllales, suggesting that AP1 orthologs involved in petal development may evolve independently across different clades of core eudicots. Our results also suggest that FaesAP1 holds potential for biotechnical engineering to develop early flowering varieties of F. esculentum.

The sweetpotato ADP-glucose pyrophosphorylase gene (ibAGP1) promoter confers high-level expression of the GUS reporter gene in the potato tuber

2009 ◽  
Vol 332 (10) ◽  
pp. 876-885 ◽  
Author(s):  
Tae-Won Kim ◽  
Young-Min Goo ◽  
Cheol-Ho Lee ◽  
Byung-Hyun Lee ◽  
Jung-Myung Bae ◽  
...  
Keyword(s):  
Potato Tuber ◽  
Reporter Gene ◽  
High Level Expression ◽  
Gus Reporter Gene ◽  
Gus Reporter ◽  
Adp Glucose Pyrophosphorylase ◽  
High Level

scholarly journals Temporal and Spatial Expression of Arabidopsis Gene Homologs Control Daylength Adaptation and Bulb Formation in Onion (Allium cepa L.)

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Md. Harun Ar Rashid ◽  
Wei Cheng ◽  
Brian Thomas
Keyword(s):  
Genetic Regulation ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Bulb Formation ◽  
Photoperiodic Regulation ◽  
Allium Cepa L ◽  
New Varieties ◽  
Large Genome Size ◽  
High Level

Abstract Genetic studies aimed at onion improvement have been limited because of high heterozygosity, a very large genome size with a high level of repetitive DNA and a biennial life cycle. Onion bulb initiation is daylength-dependent, which places a significant barrier to adapting new varieties for growth at different latitudes. Compared to the photoperiodic regulation of flowering, relatively little is known about genetic regulation of the bulbing process. This study aims to identify the role of gene sequences involved in daylength-regulated bulb formation and tissue specific expression of onion. A comprehensive set of developmental and spatial quantitative mRNA expression experiments were carried out to investigate expression of onion FLOWERING LOCUS T (AcFT), LEAFY (AcLFY) and GIBBERELLIN-3 OXIDASE (GA3ox1) during the bulbing response. Bulbing ratios were used to measure the response of onion plants under long day (LD) and short day (SD) conditions. AcFT1 was expressed in LD, which induces bulb formation, while AcFT4 was expressed in SD, which inhibits bulb formation. AcFT5 and AcFT6 were expressed in LD and might also be involved in bulb formation itself. All AcFT, AcLFY and GA3ox1 genes showed distinctive patterns of tissue specific expression in onion, with AcFT genes found primarily in the sites of perception in the leaf and LFY in the basal tissues, the site of response. The results are consistent with AcFT1 expression being the signal for LD-induced bulb initiation and AcFT4, being involved in suppressing bulbing in SD.

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