Antibacterial activity of Lantana camara L. against multidrug resistant pathogens from ICU patients of a teaching hospital

2013 ◽  
Vol 3 (2) ◽  
pp. 65-75 ◽  
Author(s):  
Debasmita Dubey ◽  
Rabindra N. Padhy
Keyword(s):  
Antibacterial Activity ◽  
Teaching Hospital ◽  
Multidrug Resistant ◽  
Lantana Camara ◽  
Icu Patients ◽  
Multidrug Resistant Pathogens

scholarly journals Synergistic antibacterial effects of colistin in combination with aminoglycoside, carbapenems, cephalosporins, fluoroquinolones, tetracyclines, fosfomycin, and piperacillin on multidrug resistant Klebsiella pneumoniae isolates

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0244673
Author(s):  
Julalak C. Ontong ◽  
Nwabor F. Ozioma ◽  
Supayang P. Voravuthikunchai ◽  
Sarunyou Chusri
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Antibacterial Effects ◽  
Drug Resistant Bacteria ◽  
Multidrug Resistant Pathogens ◽  
Conventional Antibiotics ◽  
Global Health Problem

Multidrug resistant Enterobacterales have become a serious global health problem, with extended hospital stay and increased mortality. Antibiotic monotherapy has been reported ineffective against most drug resistant bacteria including Klebsiella pneumoniae, thus encouraging the use of multidrug therapies as an alternative antibacterial strategy. The present works assessed the antibacterial activity of colistin against K. pneumoniae isolates. Resistant isolates were tested against 16 conventional antibiotics alone and in combination with colistin. The results revealed that all colistin resistant isolates demonstrated multidrug resistance against the tested antibiotics except amikacin. At sub-inhibitory concentrations, combinations of colistin with amikacin, or fosfomycin showed synergism against 72.72% (8 of 11 isolates). Colistin with either of gentamicin, meropenem, cefoperazone, cefotaxime, ceftazidime, moxifloxacin, minocycline, or piperacillin exhibited synergism against 81.82% (9 of 11 isolates). Combinations of colistin with either of tobramycin or ciprofloxacin showed synergism against 45.45% (5 in 11 isolates), while combinations of colistin with imipenem or ceftolozane and tazobactam displayed 36.36% (4 of 11 isolates) and 63.64% (7 of 11 isolates) synergism. In addition, combinations of colistin with levofloxacin was synergistic against 90.91% (10 of 11 isolates). The results revealed that combinations of colistin with other antibiotics could effectively inhibit colistin resistant isolates of K. pneumoniae, and thus could be further explore for the treatment of multidrug resistant pathogens.

scholarly journals Carbon Nanomaterials and LED Irradiation as Antibacterial Strategies against Gram-Positive Multidrug-Resistant Pathogens

2019 ◽  
Vol 20 (14) ◽  
pp. 3603 ◽  
Author(s):  
Lisa Elias ◽  
Rafael Taengua ◽  
Belén Frígols ◽  
Beatriz Salesa ◽  
Ángel Serrano-Aroca
Keyword(s):  
Antibacterial Activity ◽  
Carbon Nanomaterials ◽  
Multidrug Resistant ◽  
Gram Positive ◽  
Methicillin Resistant ◽  
Light Emitting ◽  
High Antibacterial Activity ◽  
Wide Range ◽  
Multidrug Resistant Pathogens ◽  
Led Irradiation

Background: Due to current antibiotic resistance worldwide, there is an urgent need to find new alternative antibacterial approaches capable of dealing with multidrug-resistant pathogens. Most recent studies have demonstrated the antibacterial activity and non-cytotoxicity of carbon nanomaterials such as graphene oxide (GO) and carbon nanofibers (CNFs). On the other hand, light-emitting diodes (LEDs) have shown great potential in a wide range of biomedical applications. Methods: We investigated a nanotechnological strategy consisting of GO or CNFs combined with light-emitting diod (LED) irradiation as novel nanoweapons against two clinically relevant Gram-positive multidrug-resistant pathogens: methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus epidermidis (MRSE). The cytotoxicity of GO and CNFs was studied in the presence of human keratinocyte HaCaT cells. Results: GO or CNFs exhibited no cytotoxicity and high antibacterial activity in direct contact with MRSE and MRSA cells. Furthermore, when GO or CNFs were illuminated with LED light, the MRSE and MRSA cells lost viability. The rate of decrease in colony forming units from 0 to 3 h, measured per mL, increased to 98.5 ± 1.6% and 95.8 ± 1.4% for GO and 99.5 ± 0.6% and 99.7 ± 0.2% for CNFs. Conclusions: This combined antimicrobial approach opens up many biomedical research opportunities and provides an enhanced strategy for the prevention and treatment of Gram-positive multidrug-resistant infections.

Feasibility of Routinely Using Hydrogen Peroxide Vapor to Decontaminate Rooms in a Busy United States Hospital

2009 ◽  
Vol 30 (6) ◽  
pp. 574-577 ◽  
Author(s):  
Jonathan A. Otter ◽  
Matthew Puchowicz ◽  
David Ryan ◽  
James A. G. Salkeld ◽  
Timothea A. Cooper ◽  
...  
Keyword(s):  
United States ◽  
Hydrogen Peroxide ◽  
Teaching Hospital ◽  
Multidrug Resistant ◽  
Occupancy Rate ◽  
Multidrug Resistant Pathogens ◽  
Time Required ◽  
Mean Time ◽  
Hydrogen Peroxide Vapor

During a 22-month period at a 500-bed teaching hospital, 1,565 rooms that had housed patients infected with multidrug-resistant pathogens were decontaminated using hydrogen peroxide vapor. Hydrogen peroxide vapor decontamination required a mean time of 2 hours and 20 minutes, compared with 32 minutes for conventional cleaning. Despite the greater time required for decontamination, hydrogen peroxide vapor decontamination of selected patient rooms is feasible in a busy hospital with a mean occupancy rate of 94%.

scholarly journals In-Vitro Antibacterial Activity of Probiotic Against Human Multidrug Resistant Pathogens

2020 ◽  
Vol 03 (01) ◽  
Author(s):  
Bhuvan Saud ◽  
Pratima Pandey ◽  
Govinda Paudel ◽  
Gunaraj Dhungana ◽  
Vikram Shrestha
Keyword(s):  
Antibacterial Activity ◽  
Multidrug Resistant ◽  
In Vitro Antibacterial Activity ◽  
Multidrug Resistant Pathogens

scholarly journals Synergistic Effect between Cryptotanshinone and Antibiotics against Clinic Methicillin and Vancomycin-ResistantStaphylococcus aureus

2014 ◽  
Vol 2014 ◽  
pp. 1-16 ◽  
Author(s):  
Jeong-Dan Cha ◽  
Jeong-Ho Lee ◽  
Kyung Min Choi ◽  
Sung-Mi Choi ◽  
Jeong Hye Park
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Medicinal Plant ◽  
Synergistic Effect ◽  
Antibacterial Agent ◽  
Salvia Miltiorrhiza ◽  
Multidrug Resistant ◽  
Vancomycin Resistant ◽  
Multidrug Resistant Pathogens ◽  
Time Kill

Cryptotanshinone (CT), a major tanshinone of medicinal plantSalvia miltiorrhizaBunge, demonstrated strong antibacterial activity against clinic isolated methicillin and vancomycin-resistantStaphylococcus aureus(MRSA and VRSA) in this experiment. The CT was determined against clinic isolated MRSA 1–16 with MIC and MBC values ranging from 4 to 32 and 8 to 128 μg/mL; for MSSA 1-2 from 16 to 32 μg/mL and 64 to 128 μg/mL; for VRSA 1-2 from 2 to 4 μg/mL and 4 to 16 μg/mL, respectively. The range of MIC50and MIC90of CT was 0.5–8 μg/mL and 4–64 μg/mL, respectively. The combination effects of CT with antibiotics were synergistic (FIC index<0.5) against most of tested clinic isolated MRSA, MSSA, and VRSA except additive, MRSA 4 and 16 in oxacillin, MRSA 6, 12, and 15 in ampicillin, and MRSA 6, 11, and 15 in vancomycin (FIC index < 0.75–1.0). Furthermore, a time-kill study showed that the growth of the tested bacteria was completely attenuated after 2–6 h of treatment with the 1/2 MIC of CT, regardless of whether it was administered alone or with ampicillin, oxacillin, or vancomycin. The results suggest that CT could be employed as a natural antibacterial agent against multidrug-resistant pathogens infection.

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