Characterization of buttermilk serum fractions and their effect on rennet-induced coagulation of casein micelle dispersions

2018 ◽  
Vol 76 ◽  
pp. 10-17 ◽  
Author(s):  
Marie-Pierre Gauvin ◽  
Yves Pouliot ◽  
Michel Britten
Keyword(s):  
Casein Micelle ◽  
Serum Fractions

scholarly journals Characterization of Apolipoprotein E-containing Lipoproteins in Cerebrospinal Fluid: Effect of Phenotype on the Distribution of Apolipoprotein E

1999 ◽  
Vol 45 (9) ◽  
pp. 1431-1438 ◽  
Author(s):  
Kazuyoshi Yamauchi ◽  
Minoru Tozuka ◽  
Hiroya Hidaka ◽  
Eiko Hidaka ◽  
Yoshiyuki Kondo ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Apolipoprotein E ◽  
Gel Filtration ◽  
Distribution Profile ◽  
Serum Fractions ◽  
Apo E ◽  
The Central Nervous System ◽  
Unique Composition ◽  
Apoe Phenotype

Abstract Background: Apolipoprotein (apo) E, one of the main apolipoproteins in the central nervous system, may play an important role in lipid metabolism; however, the details of its function are poorly understood. In this study, we characterized apoE-containing lipoproteins in cerebrospinal fluid (CSF) and examined the effect of apoE phenotype on the distribution of apoE among the lipoprotein fractions. Methods: CSF lipoproteins were fractionated by gel filtration and ultracentrifugation, and then characterized by electrophoresis, immunoblot, electron microscopy, and analysis of apoE, total cholesterol, and phospholipid concentrations. Results: The ratio of sialylated to nonsialylated apoE was higher in CSF than in serum. However, the fundamental forms containing apoE homodimers or heterodimers [such as apo(E-AII) and apo(AII-E2-AII) complexes] were similar in CSF and serum. apoE-containing lipoproteins were fractionated at densities of <1.006, 1.063–1.125, and 1.125–1.21 kg/L. Neither apoE nor apoAI was detected in the fraction with a density range of 1.006–1.063 kg/L. The diameters of the lipoprotein particles with densities of <1.006, 1.063–1.125, and 1.125–1.21 kg/L were 16.7 ± 3.1, 14.0 ± 3.2, and 11.6 ± 2.8 nm (mean ± SD, n = 200), respectively. All of these lipoproteins exhibited a spherical structure. The distribution profile of apoE-containing lipoproteins was affected by the apoE phenotype. A relatively large amount of apoE-containing lipoproteins was isolated from the fraction with a density >1.125 kg/L obtained from CSF associated with apoE2 or apoE3. This tendency was more obvious in CSF associated with apoE2 than in CSF without apoE2. apoE-containing lipoproteins were predominantly observed in the fraction with a density of <1.006 kg/L obtained from CSF associated with apoE4. Conclusions: The lipoproteins in CSF have a unique composition that is different from that of the lipoproteins in plasma. However, the differences in diameter between the CSF fractions were not as large as for the serum fractions. Our data suggest that the apoE phenotype may affect the distribution profile of apoE-containing lipoproteins in the CSF. This would mean that the metabolism of apoE-containing lipoproteins depends on the apoE isoform present.

scholarly journals Characterization of Casein Micelle Precipitation by Chitosans

2001 ◽  
Vol 84 (2) ◽  
pp. 361-369 ◽  
Author(s):  
S.F. Ausar ◽  
I.D. Bianco ◽  
R.G. Badini ◽  
L.F. Castagna ◽  
N.M. Modesti ◽  
...  
Keyword(s):  
Casein Micelle

scholarly journals Characterization of Maternal Isoagglutinins in ABO Hemolytic Disease of the Newborn

Blood ◽  
1966 ◽  
Vol 27 (1) ◽  
pp. 17-37 ◽  
Author(s):  
SUSIE W. FONG ◽  
ARLENE NUCKTON ◽  
H. H. FUDENBERG
Keyword(s):  
Density Gradient ◽  
Blood Group ◽  
Density Gradient Ultracentrifugation ◽  
Hemolytic Disease ◽  
Test Systems ◽  
Serum Fractions ◽  
Diethylaminoethyl Cellulose ◽  
Abo Hemolytic Disease

Abstract Sera of group O mothers of infants with and without ABO hemolytic disease of the newborn were fractionated by diethylaminoethyl-cellulose chromatography and density gradient ultracentrifugation. Whole sera and serum fractions were evaluated for activity by various test systems currently used for the antenatal prediction of ABO hemolytic disease of the newborn. The results of these studies did not show any single serologic property that would unequivocally diflerentiate between the isoantibodies of mothers of affected infants and mothers of normal infants of incompatible blood group.

Epitope characterization of a supramolecular protein assembly with a collection of monoclonal antibodies: The case of casein micelle

2009 ◽  
Vol 46 (6) ◽  
pp. 1058-1066 ◽  
Author(s):  
Annette Johansson ◽  
Damien Lugand ◽  
Odile Rolet-Répécaud ◽  
Daniel Mollé ◽  
Marie-Madeleine Delage ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Protein Assembly ◽  
Casein Micelle ◽  
Epitope Characterization

scholarly journals Characterization of the dromedary milk casein micelle and study of its changes during acidification

2000 ◽  
Vol 80 (5) ◽  
pp. 503-515 ◽  
Author(s):  
Hamadi Attia ◽  
Naouel Kherouatou ◽  
Moncef Nasri ◽  
Touhami Khorchani
Keyword(s):  
Casein Micelle ◽  
Milk Casein ◽  
Dromedary Milk

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

AEM analysis of crystallization in Ti-based amorphous alloys

1983 ◽  
Vol 41 ◽  
pp. 270-271
Author(s):  
A.R. Pelton ◽  
A.F. Marshall ◽  
Y.S. Lee
Keyword(s):  
Magnetic Properties ◽  
Amorphous Alloys ◽  
Amorphous Materials ◽  
Isothermal Annealing ◽  
Amorphous Matrix ◽  
Nucleation And Growth ◽  
Current Interest ◽  
Amorphous Films ◽  
Electrical And Magnetic Properties

Amorphous materials are of current interest due to their desirable mechanical, electrical and magnetic properties. Furthermore, crystallizing amorphous alloys provides an avenue for discerning sequential and competitive phases thus allowing access to otherwise inaccessible crystalline structures. Previous studies have shown the benefits of using AEM to determine crystal structures and compositions of partially crystallized alloys. The present paper will discuss the AEM characterization of crystallized Cu-Ti and Ni-Ti amorphous films.Cu60Ti40: The amorphous alloy Cu60Ti40, when continuously heated, forms a simple intermediate, macrocrystalline phase which then transforms to the ordered, equilibrium Cu3Ti2 phase. However, contrary to what one would expect from kinetic considerations, isothermal annealing below the isochronal crystallization temperature results in direct nucleation and growth of Cu3Ti2 from the amorphous matrix.

Characterization of Coherent, Ordered Precipitates in Nickel-Base Alloys

1973 ◽  
Vol 31 ◽  
pp. 144-145
Author(s):  
B. H. Kear ◽  
J. M. Oblak
Keyword(s):  
Stable Phase ◽  
Nickel Base Superalloy ◽  
Alloy 718 ◽  
Commercial Alloy ◽  
Precipitate Morphology ◽  
Continuous Precipitation ◽  
Nickel Base ◽  
Base Superalloy ◽  
Strengthening Precipitate

A nickel-base superalloy is essentially a Ni/Cr solid solution hardened by additions of Al (Ti, Nb, etc.) to precipitate a coherent, ordered phase. In most commercial alloy systems, e.g. B-1900, IN-100 and Mar-M200, the stable precipitate is Ni3 (Al,Ti) γ′, with an LI2structure. In A lloy 901 the normal precipitate is metastable Nis Ti3 γ′ ; the stable phase is a hexagonal Do2 4 structure. In Alloy 718 the strengthening precipitate is metastable γ″, which has a body-centered tetragonal D022 structure.Precipitate MorphologyIn most systems the ordered γ′ phase forms by a continuous precipitation re-action, which gives rise to a uniform intragranular dispersion of precipitate particles. For zero γ/γ′ misfit, the γ′ precipitates assume a spheroidal.

The Use of Advanced Analytical Techniques for Characterization of the Chemical, Physical, and Crystallographic Nature of a Surface

1973 ◽  
Vol 31 ◽  
pp. 132-133 ◽  
Author(s):  
R. E. Herfert
Keyword(s):  
Surface Characterization ◽  
Analytical Techniques ◽  
X Ray Diffraction ◽  
Depth Of Penetration ◽  
X Ray ◽  
The Past ◽  
Transmission Electron ◽  
Scanning Electron

Studies of the nature of a surface, either metallic or nonmetallic, in the past, have been limited to the instrumentation available for these measurements. In the past, optical microscopy, replica transmission electron microscopy, electron or X-ray diffraction and optical or X-ray spectroscopy have provided the means of surface characterization. Actually, some of these techniques are not purely surface; the depth of penetration may be a few thousands of an inch. Within the last five years, instrumentation has been made available which now makes it practical for use to study the outer few 100A of layers and characterize it completely from a chemical, physical, and crystallographic standpoint. The scanning electron microscope (SEM) provides a means of viewing the surface of a material in situ to magnifications as high as 250,000X.

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