Molecular characterisation of methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) Staphylococcus aureus isolated from bovine subclinical mastitis and Egyptian raw milk cheese

2020 ◽  
Vol 104 ◽  
pp. 104646
Author(s):  
Mahmoud Ge. Zayda ◽  
Yoshimitsu Masuda ◽  
Ahmed M. Hammad ◽  
Ken-ichi Honjoh ◽  
Abdelrahman M. Elbagory ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Raw Milk ◽  
Subclinical Mastitis ◽  
Molecular Characterisation ◽  
Methicillin Resistant ◽  
Raw Milk Cheese

scholarly journals Prevalence of methicillin-resistant Staphylococcus aureus and pattern of antimicrobial resistance in mastitis milk of cattle in Chitwan, Nepal

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Asmita Shrestha ◽  
Rebanta Kumar Bhattarai ◽  
Himal Luitel ◽  
Surendra Karki ◽  
Hom Bahadur Basnet
Keyword(s):  
Public Health ◽  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Subclinical Mastitis ◽  
World Health ◽  
Dairy Herds ◽  
Microbiological Analysis ◽  
Biochemical Tests ◽  
Methicillin Resistant ◽  
Milk Samples

Abstract Background The threat of methicillin-resistant Staphylococcus aureus (MRSA) exists globally and has been listed as a priority pathogen by the World Health Organization. One of the sources of MRSA emergence is livestock and its products, often raised in poor husbandry conditions. There are limited studies in Nepal to understand the prevalence of MRSA in dairy animals and its antimicrobial resistance (AMR) profile. A cross-sectional study was conducted in Chitwan, one of the major milk-producing districts of Nepal, from February 2018 to September 2019 to estimate the prevalence of MRSA in milk samples and its AMR profile. The collected milk samples (n = 460) were screened using the California Mastitis Test (CMT) and positive samples were subjected to microbiological analysis to isolate and identify S. aureus. Polymerase Chain Reaction (PCR) was used to identify the presence of the mecA gene and screen for MRSA. Results In total, 41.5% (191/460) of milk samples were positive in the CMT test. Out of 191 CMT positive milk samples, the biochemical tests showed that the prevalence of S. aureus was 15.2% (29/191). Among the 29 S. aureus isolates, 6.9% (2/29) were identified as MRSA based on the detection of a mecA gene. This indicates that that 1.05% (2/191) of mastitis milk samples had MRSA. The antibiotic sensitivity test showed that 75.9% (22/29) and 48.3% (14/29) S. aureus isolates were found to be sensitive to Cefazolin and Tetracycline respectively (48.3%), whereas 100% of the isolates were resistant to Ampicillin. In total 96.6% (28/29) of S. aureus isolates were multidrug-resistant (MDR). Conclusions This study revealed a high prevalence of S. aureus-mediated subclinical mastitis in dairy herds in Chitwan, Nepal, with a small proportion of it being MRSA carrying a mecA gene. This S. aureus, CoNS, and MRSA contaminated milk poses a public health risk due to the presence of a phenotype that is resistant to very commonly used antibiotics. It is suggested that dairy herds be screened for subclinical mastitis and treatments for the animals be based on antibiotic susceptibility tests to reduce the prevalence of AMR. Furthermore, future studies should focus on the Staphylococcus spp. to explore the antibiotic resistance genes in addition to the mecA gene to ensure public health.

scholarly journals Enterotoxin-producing Staphylococcus aureus genotype B as a major contaminant in Swiss raw milk cheese

2014 ◽  
Vol 97 (3) ◽  
pp. 1305-1312 ◽  
Author(s):  
J. Hummerjohann ◽  
J. Naskova ◽  
A. Baumgartner ◽  
H.U. Graber
Keyword(s):  
Staphylococcus Aureus ◽  
Raw Milk ◽  
Raw Milk Cheese

scholarly journals ЕТІОЛОГІЧНІ ЧИННИКИ МАСТИТІВ КОРІВ УКРАЇНСЬКОЇ ЧОРНО–РЯБОЇ МОЛОЧНОЇ ПОРОДИ

2016 ◽  
Vol 18 (3(70)) ◽  
pp. 191-196
Author(s):  
V. Panevnyk ◽  
T. Suprovych
Keyword(s):  
Staphylococcus Aureus ◽  
Somatic Cells ◽  
Raw Milk ◽  
Subclinical Mastitis ◽  
Index Number ◽  
Clinical Form ◽  
Leading Role ◽  
Skin Contamination ◽  
Major Pathogens ◽  
And Staphylococcus Aureus

The article shows data on the microbial landscape and quantity of somatic cells milk in different forms of mastitis in cows. Index number of somatic cells (SCC) in the raw milk of cows in the country is only used to establish the quality milk. They are key safety indicators that are directly related to udder cow disease, especially subclinical mastitis. Research has established that the number of SCC in healthy animals ranges from 84000 cells/ml to 436000 cells/ml. Over the course of subclinical turned from 508000 cells/ml to 756000 cells/ml. Animals with clinical form of mastitis were from 876000 cells/ml to 69260000 cells/ml. The 42 cows of the first lactation average SCC was 143000 cells/ml, and 47 of fifth lactation cows – 213000 cells/ml.The microflora in the breast can get in different ways: galactogenous – through teat channel hematogenous ahd lymphogenous ways. The leading role galactogenous way in which the pathogens penetrate from the environment through teat channel. This contributes to the udder skin contamination by microorganisms. Activators of subclinical mastitis were Staphylococcus aureus 31.8% and Streptococcus agalactiae 40.9%. In the clinical course of mastitis major pathogens were Escherichia coli – 34.8% and Staphylococcus aureus – 41.3%. Selected cultures of microorganisms were sensitive to cephalexin, gentamicin, oxacillin, rifampicin, enrofloksacin. 

Coagulase gene polymorphism and antimicrobial susceptibility of Staphylococcus aureus isolated from bovine subclinical mastitis milk in Sidi-Bel-Abbes, Algeria

2017 ◽  
Vol 7 (1) ◽  
pp. 21-27
Author(s):  
Ahmed Reda Belmamoun ◽  
Abdelkader Ammam ◽  
Imene Berrabah ◽  
Karima Bereksi Reguig
Keyword(s):  
Staphylococcus Aureus ◽  
Protein A ◽  
Pcr Amplification ◽  
Screen Test ◽  
Raw Milk ◽  
Subclinical Mastitis ◽  
Latex Agglutination ◽  
Pcr Analysis ◽  
California Mastitis Test ◽  
Coa Gene

The study was conducted to identify and characterize Staphylococcus aureus in raw milk derived from subclinical mastitis in Sidi-Bel-Abbes Algeria. In this paper, we explore the possibility of detection of the coagulase gene (coa), which encodes the coagulase enzyme, by PCR analysis in antibiotic-resistant isolates, with the latex agglutination phenotype and free coagulase.Out of 336 samples of raw milk examined with California mastitis test (CMT) posi-tive; a total of 142 samples were bacteriologically positive with 56.34% Staphylococcus isolates, 21 (26.25%) isolates were confirmed as S.aureus. Nineteen (90.48%) isolates of S.aureus showed free coagulase on the tube agglutination test. Two atypical S.aureus strains (9.52%) were defective for the clumping factor and / or protein A , determined with the Staphytect plus test and the tube coagulase test. The isolates of S.aureus were resistant to penicillin and tetracycline with 76.19%. Two isolates (9.52%) of S.aureus re-sistant to meticillin (MRSA) were detected in this study, with a MIC of ≥4 μg / liter and a cefoxitin screen test with a MIC of ≥8 μg / liter, and 13 (61.9%) isolates were with a multiresistance phenotype. The 21 isolates were sub-jected to PCR amplification of the 3' end of the coa gene, 18 (85.71%) were revealed on a 1% agarose gel with a single band between 547 bp and 875 bp. The use of the PCR genotypic test to identify the profile of the coa gene can be used as an appropriate identification criterion for differentiating coagulases from S.aureus and for understanding their epidemiology.

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