In vitro biological and adhesive properties of universal adhesive systems on sound and caries-affected dentine: 18 months

Dentin hypersensitivity is caused by increased dentinal permeability due to total or partial exposure of dentinal tubules, which in turn can be produced by alterations of dental structures or failure of restorative procedures. The purpose of this in vitro study was to evaluate the efficacy of the application of different kinds of adhesive systems to prevent dentin permeability before and after an erosive challenge. Fifty bovine dentin discs (6x1 mm) were prepared and the specimens were divided into 5 groups (n=10): (SB2) Single Bond 2, (SBU) Universal Single Bond, (CSB) Clearfil SE Bond, (SM) Scotchbond Multipurpose and (C) Control. Hydraulic conductance of dentin was recorded after adhesive application (HC-1) and after erosive challenge (HC-2). Dentin surface images of post-treatment and post-erosive challenge were obtained by scanning electron microscopy (SEM). Data were analyzed using Kruskal Wallis, Mann-Whitney with Bonferroni correction and Wilcoxon tests (p<0.05). Reduction in dentin permeability was observed with the application of adhesive systems (p<0.05). After the erosive challenge, dentin permeability increased for SBU and CSB (p<0.05), while SB2 and SM did not differ in HC-1 or HC-2 (p>0.05). The conventional, self-etching and universal adhesive systems reduce dentinal permeability by more than 80%, and dentin demineralization may contribute to the increased permeability of universal and self-etching systems.

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In Vitro Effect of Temperature on Dentin Bond Strength of Universal Adhesive Systems

Odovtos - International Journal of Dental Sciences ◽

10.15517/ijds.2020.39198 ◽

2019 ◽

pp. 253-261

Author(s):

Serdar Akarsu DDS, PhD ◽

Sultan Aktuğ Karademir DDS

Keyword(s):

Bond Strength ◽

Adhesive System ◽

Effect Of Temperature ◽

Single Bond ◽

Adhesive Systems ◽

Universal Adhesive ◽

Significant Difference ◽

Universal Adhesives ◽

Vitro Effect

The aim of this study was to evaluate the shear bond strength (SBS) of two universal adhesives (Universal Single Bond and All Bond Universal) and a two-step self-etch adhesive system (Clearfil SE Bond) to dentine at various temperatures. Materials and Methods: One hundred and twenty dentin specimens were divided randomly to 12 groups, according to adhesive systems (Universal Single Bond and All Bond Universal, Clearfil SE Bond) and temperature ( 4ºC, 20 ºC, 36ºC, 55ºC) used. Dentin specimens were prepared (n :10, adhesives were applied, and composite cylinders were polymerized. Statistical analysis of the SBS data was performed using Two-way analysis of variance (ANOVA) and Tukey’s Honestly Significant Differences post-hoc test. Results: The Clearfil SE Bond was shown to have higher SBS than the universal adhesives at all temperatures; however, there was no statistically significant difference (P>0.05). In both groups, the lowest SBS values were observed in the samples at 4°C while the highest SBS values were observed in the samples at 55°C. In this case, there was a statistically significant difference (P<0.05). Conclusions: The results suggest that the effectiveness of an adhesive may increase if it is preheated at 36°C or above before use instead of being used immediately after removal from the refrigerator or at room temperature.

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Does Adhesive Layer Thickness and Tag Length Influence Short/Long-Term Bond Strength of Universal Adhesive Systems? An In-Vitro Study

Applied Sciences ◽

10.3390/app11062635 ◽

2021 ◽

Vol 11 (6) ◽

pp. 2635

Author(s):

Naji Kharouf ◽

Tarek Ashi ◽

Ammar Eid ◽

Levi Maguina ◽

Jihed Zghal ◽

...

Keyword(s):

Bond Strength ◽

Layer Thickness ◽

Storage Period ◽

Adhesive Layer ◽

Adhesive Systems ◽

Universal Adhesive ◽

Universal Adhesives ◽

Self Etch ◽

Over Time

(1) Background: This study investigated the effect of the adhesive layer thickness and the length of resin tags on dentin bond strength of five universal adhesives applied in self-etch mode. (2) Methods: One hundred and fifty extracted human third molars were used. Five different universal adhesives were applied in self-etch mode on the dentin surface. Half of the specimens were subjected to an aging procedure for six months. A shear bond strength (SBS) test was performed and the results were statistically analyzed with a t-test and one-way ANOVA test. Scanning electron microscopy (SEM) was executed to measure the adhesive layer thickness and tag depth. (3) Results: No statistical differences were found between the five adhesive systems after a 24 h storage period, regardless of layer thickness and tag depth (p < 0.05). After 6 months of aging in water at 37 °C, Iperbond Max and Scotchbond Universal preserved the bond strength over time (p < 0.05), whilst the SBS of Iperbond Ultra, FuturaBond M+, and Ibond Universal decreased significantly after the aging period. No relation was observed between the adhesive thickness or tags’ length on SBS. (4) Conclusions: Within the limitation of this study, the stability over time of the bond strength of universal adhesives depends on their compositions regardless of the adhesive layer thickness and/or tags’ length.

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Does the etching strategy influence the bonding of universal adhesive systems to primary teeth? A systematic review and meta-analysis of in vitro studies

European Archives of Paediatric Dentistry ◽

10.1007/s40368-021-00639-w ◽

2021 ◽

Author(s):

T. T. Fröhlich ◽

L. D. Gindri ◽

F. Z. M. Soares ◽

R. de Oliveira Rocha

Keyword(s):

Systematic Review ◽

Primary Teeth ◽

Meta Analysis ◽

In Vitro Studies ◽

Adhesive Systems ◽

Universal Adhesive

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In vitro comparison of the immediate adhesive strength in dentin of three universal adhesive systems.

Journal Of Oral Research ◽

10.17126/joralres.2018.044 ◽

2018 ◽

Vol 7 (5) ◽

pp. 173-177

Author(s):

Mariana Acurio-Benavente ◽

◽

Haru Kinoshita-Rivas ◽

Paloma Acurio-Benavente ◽

Leslie Casas-Apayco ◽

...

Keyword(s):

Adhesive Strength ◽

Adhesive Systems ◽

Universal Adhesive ◽

In Vitro Comparison

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Differences in a Single Extracellular Residue Underlie Adhesive Functions of Two Zebrafish Aqp0s

Cells ◽

10.3390/cells10082005 ◽

2021 ◽

Vol 10 (8) ◽

pp. 2005

Author(s):

Irene Vorontsova ◽

James E. Hall ◽

Thomas F. Schilling ◽

Noriaki Nagai ◽

Yosuke Nakazawa

Keyword(s):

Cell Adhesion ◽

Single Amino Acid ◽

Amino Acid Difference ◽

Adhesion Assay ◽

Loss Of Function ◽

Adhesive Properties ◽

The Difference ◽

In Vitro Adhesion ◽

Cell To Cell Adhesion

Aquaporin 0 (AQP0) is the most abundant lens membrane protein, and loss of function in human and animal models leads to cataract formation. AQP0 has several functions in the lens including water transport and adhesion. Since lens optics rely on strict tissue architecture achieved by compact cell-to-cell adhesion between lens fiber cells, understanding how AQP0 contributes to adhesion would shed light on normal lens physiology and pathophysiology. We show in an in vitro adhesion assay that one of two closely related zebrafish Aqp0s, Aqp0b, has strong auto-adhesive properties while Aqp0a does not. The difference appears to be largely due to a single amino acid difference at residue 110 in the extracellular C-loop, which is T in Aqp0a and N in Aqp0b. Similarly, P110 is the key residue required for adhesion in mammalian AQP0, highlighting the importance of residue 110 in AQP0 cell-to-cell adhesion in vertebrate lenses as well as the divergence of adhesive and water permeability functions in zebrafish duplicates.

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Retention of leukocytes in capillaries: role of cell size and deformability

Journal of Applied Physiology ◽

10.1152/jappl.1990.69.5.1767 ◽

1990 ◽

Vol 69 (5) ◽

pp. 1767-1778 ◽

Cited By ~ 109

Author(s):

G. P. Downey ◽

D. E. Doherty ◽

B. Schwab ◽

E. L. Elson ◽

P. M. Henson ◽

...

Keyword(s):

Cell Size ◽

Dynamic Equilibrium ◽

Human Neutrophils ◽

Biophysical Properties ◽

Adhesive Properties ◽

Pulmonary Capillaries ◽

Size Discrepancy ◽

Capillary Bed ◽

Filtration Properties

Leukocytes within the circulation are in dynamic equilibrium with a marginated pool, thought to reside mainly within the pulmonary capillaries. The size discrepancy between the mean diameter of circulating leukocytes (6-8 microns) and that of the pulmonary capillaries (approximately 5.5 microns) forces the cells to deform in order to transit the capillary bed. Consequently, we investigated the hypothesis that the biophysical properties of cell size and deformability determined differential leukocyte retention in the lung. Comparison of the filtration properties of human neutrophils, lymphocytes, monocytes, platelets, and erythrocytes through polycarbonate filters (5-micron pore diameter) revealed that the largest leukocytes (neutrophils and monocytes) were retained to the greatest extent and the smaller cells (lymphocytes and platelets) the least. Undifferentiated HL-60 cells, of greater diameter than their differentiated counterparts, were also retained to a greater extent, confirming that cell size was one important determinant of retention in these model capillaries. However, compared with neutrophils, which are of similar diameter, monocytes were retained to a greater extent, suggesting that monocytes might be less deformable than neutrophils. To test this hypothesis, deformability was measured directly using the cell poker. Monocytes were found to be the stiffest, neutrophils the softest, and lymphocytes intermediate. Glutaraldehyde treatment of neutrophils markedly increased their stiffness and decreased their ability to transit the pores of the filters in vitro and the pulmonary microvasculature of rabbits without changing their adhesive properties or size. These observations support the hypothesis that biophysical properties of leukocytes (size and deformability) determine in part their ability to transit the pulmonary capillaries and may determine the magnitude of their marginated pools.

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Quantitative evaluation of microleakage in class V cavities using one-bottle and self-etching adhesive systems

Brazilian Oral Research ◽

10.1590/s1806-83242004000300014 ◽

2004 ◽

Vol 18 (3) ◽

pp. 253-259 ◽

Cited By ~ 2

Author(s):

Fabiana Mantovani Gomes França ◽

Flávio Henrique Baggio Aguiar ◽

Alex José Souza dos Santos ◽

José Roberto Lovadino

Keyword(s):

Methylene Blue ◽

In Vitro Study ◽

Adhesive System ◽

Acid Etching ◽

Single Bond ◽

Adhesive Systems ◽

Class V ◽

Bonding System ◽

Root Dentin

The aim of this in vitro study was to evaluate quantitatively the microleakage in class V cavities restored with one-bottle and self-etching adhesive systems with and without previous acid etching. Two one-bottle adhesive systems (Single Bond and Prime & Bond 2.1) and one self-etching adhesive system (Clearfil Mega Bond) were used in this study. One hundred and twenty sound human premolar teeth were randomly divided into 6 groups, and 20 class V restorations were prepared in the root dentin to test each bonding system. Each bonding system was used with and without acid etching. Specimens were prepared, dyed with 2% methylene blue, sectioned, triturated, and evaluated with an absorbance spectrophotometer test in order to quantify the infiltrated dye. Results were statistically evaluated by ANOVA and Tukey-Kramer test. No statistically significant differences were found among the adhesive systems when no etching agent was used. However, the Single Bond adhesive system showed statistically significant lower microleakage means than Clearfil Mega Bond and Prime & Bond 2.1 when 37% phosphoric acid was used. Single Bond and Clearfil Mega Bond adhesive systems presented similar behavior when the manufacturers' instructions were followed.

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