Augmented anticancer activity of curcumin loaded fungal chitosan nanoparticles

2020 ◽  
Vol 155 ◽  
pp. 861-867 ◽  
Author(s):  
Fahad M. Almutairi ◽  
Haddad A. El Rabey ◽  
Ahmed A. Tayel ◽  
Adel I. Alalawy ◽  
Mohammed A. Al-Duais ◽  
...  
Keyword(s):  
Anticancer Activity ◽  
Chitosan Nanoparticles ◽  
Fungal Chitosan

Effect of co-treatment with doxorubicin and verapamil loaded into chitosan nanoparticles on diethylnitrosamine-induced hepatocellular carcinoma in mice

2020 ◽  
Vol 39 (11) ◽  
pp. 1528-1544 ◽  
Author(s):  
HE Abo Mansour ◽  
MM El-Batsh ◽  
NS Badawy ◽  
ET Mehanna ◽  
NM Mesbah ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Anticancer Activity ◽  
Hepg2 Cells ◽  
Chitosan Nanoparticles ◽  
B Cell Lymphoma ◽  
Favorable Effect ◽  
Vascular Endothelial ◽  
Factor Α ◽  
Endothelial Growth Factor

This study aimed to investigate the potential role of co-treatment with doxorubicin (DOX) and verapamil (VRP) nanoparticles in experimentally induced hepatocellular carcinoma in mice and to investigate the possible mechanisms behind the potential favorable effect of the co-treatment. DOX and VRP were loaded into chitosan nanoparticles (CHNPs), and cytotoxicity of loaded and unloaded drugs against HepG2 cells was evaluated. Male albino mice were divided into eight groups ( n = 15): (1) normal control, (2) diethylnitrosamine, (3) CHNPs, (4) free DOX, (5) CHNPs DOX, (6) free VRP, (7) CHNPs VRP, and (8) CHNPs DOX + CHNPs VRP. Either VRP or DOX loaded into CHNPs showed stronger growth inhibition of HepG2 cells than their free forms. DOX or VRP nanoparticles displayed pronounced anticancer activity in vivo through the decline of vascular endothelial growth factor and B cell lymphoma-2 contents in liver tissues, upregulation of antioxidant enzymes, and downregulation of multidrug resistance 1. Moreover, reduced cardiotoxicity was evident from decreased level of tumor necrosis factor-α and malondialdehyde in heart tissues coupled with decreased serum activity of creatine kinase-myocardial band and lactate dehydrogenase. Co-treatment with CHNPs DOX and CHNPs VRP showed superior results versus other treatments. Liver sections from the co-treatment group revealed the absence of necrosis, enhanced apoptosis, and nearly normal hepatic lobule architecture. Co-treatment with CHNPs DOX and CHNPs VRP revealed enhanced anticancer activity and decreased cardiotoxicity versus the corresponding free forms.

Effects of fungal chitosan nanoparticles as eco-friendly edible coatings on the quality of postharvest table grapes

2018 ◽  
Vol 139 ◽  
pp. 56-66 ◽  
Author(s):  
Natália Ferrão Castelo Branco Melo ◽  
Bruna Lúcia de MendonçaSoares ◽  
Katharina Marques Diniz ◽  
Camila Ferreira Leal ◽  
Darllety Canto ◽  
...  
Keyword(s):  
Chitosan Nanoparticles ◽  
Edible Coatings ◽  
Table Grapes ◽  
Fungal Chitosan

Augmented control of drug-resistant Candida spp. via fluconazole loading into fungal chitosan nanoparticles

2019 ◽  
Vol 141 ◽  
pp. 511-516 ◽  
Author(s):  
Haddad A. El Rabey ◽  
Fahad M. Almutairi ◽  
Adel I. Alalawy ◽  
Mohammed A. Al-Duais ◽  
Mohamed I. Sakran ◽  
...  
Keyword(s):  
Chitosan Nanoparticles ◽  
Drug Resistant ◽  
Candida Spp ◽  
Fungal Chitosan

Design Graph Theoretical Analysis and In Silico Modeling of Dunaliella Bardawil Biomass Encapsulated N-Succinyl Chitosan Nanoparticles for Enhanced Anticancer Activity

2019 ◽  
Vol 18 (13) ◽  
pp. 1900-1918 ◽  
Author(s):  
Selvaraj Kunjiappan ◽  
Theivendren Panneerselvam ◽  
Balasubramanian Somasundaram ◽  
Murugesan Sankaranarayanan ◽  
Pavadai Parasuraman ◽  
...  
Keyword(s):  
Particle Size ◽  
Molecular Docking ◽  
Anticancer Activity ◽  
Signaling Pathway ◽  
Pathway Analysis ◽  
Chitosan Nanoparticles ◽  
Docking Study ◽  
Molecular Docking Study ◽  
Dunaliella Bardawil ◽  
Ras P21

Purpose: To investigate N-succinyl chitosan nanoparticles (NSC NPs) encapsulation with Dunaliella bardawil (D. bardawil) biomass for high utilization enhanced effectiveness and least side effects for anticancer activity. Methods: The potential bioactive compounds from D. bardawil biomass were encapsulated NSC NPs by ionotropic gelation method and to characterize its molecular shape, particle size, stability and polydispersity index using FTIR, XRD, SEM, TEM and Zetasize Nano analyzer. Signaling pathway analysis, molecular docking study and in vitro anticancer screening were performed on chosen H-Ras P21, 721P and liver cancer cell lines (HepG2), respectively. Results: The D. bardawil biomass majorly contains 6 bioactive compounds such as β-carotene, lutein, zeaxanthin, phytoene, canthaxanthin, and phytofluene were identified by LC-MS. The D. bardawil biomass encapsulated NSC NPs showed an average particle size of 80±5.6 nm in spherical shape, crystalline nature, zeta potential of -32±2.7 mV and polydispersity index of 0.51±0.02. Interestingly, the identified target using graph theoretical signaling pathway analysis and molecular docking study showed strong interaction of NSC NPs in binding pockets of H-Ras P21 protooncogene. At 50μg/mL, NPs displayed 95.60% cytotoxicity in HepG2 cell line. The apoptotic cell cycle analysis showed cell death for 24 h and 48 h representing 13.13% and 47.04%, respectively. Conclusion: The highly cross-linked, biocompatible, biodegradable, nontoxic NSC NPs promising carrier for delivery of bioactive molecules present in the D. bardawil biomass was found to be actively involved in deregulation of cellular growth in targeted cancer cells. Thus active NPs serve as a novel nanodrug to enhance the controlled; site specific drug delivery in the management of cancer.

Extraction and characterization of fungal chitosan and voriconazole loaded chitosan antifungal nanoformulations

2021 ◽  
Author(s):  
Faheem Khawaja ◽  
Zia Ullah Shah ◽  
Muhammad Nadeem
Keyword(s):  
Aspergillus Niger ◽  
Mycelial Growth ◽  
Functional Group ◽  
Group Composition ◽  
Chitosan Nanoparticles ◽  
Maximum Growth ◽  
Antifungal Drugs ◽  
Nano Drug Delivery ◽  
Fungal Chitosan

Abstract This study aimed at preparing chitosan nanoparticles (C.N.P.s) from fungal Chitosan for the purpose of producing nano-drug delivery systems for antifungal drugs. Chitosan was extracted from mycelia of Aspergillus niger using modified methods of George et al., (2011) and (Naghdi et al., 2014). For confirmation, the FTIR spectrum of the isolated Chitosan was compared with standard. A nanoformulation for Voriconazole antifungal drug was synthesized via ionic gelation method and characterized for their particle size, polydispersity index, surface charge, functional group composition using and Malvern Zeta Sizer ZS-90, P.C.S., FTIR, S.E.M. The prepared nanoformulation was also checked for its antifungal activity using Aspergillus niger and Aspergillus fumigatus as an indicator organism. Voriconazole showed maximum growth with no mycelial growth while CNPS showed minimum growth of about 70 percent, and Voriconazole loaded CNPS showed inhibitory growth effects on the mycelial growth of A. niger and A. fumigatus. Our results indicated that Chitosan Nanoparticles have huge potential against fungal diseases caused by Aspergillus species.

scholarly journals Crude and Chitosan Nano-particles Extracts of Some Maggots as Antioxidant and Anticancer Agents

2019 ◽  
pp. 1-8 ◽  
Author(s):  
Ahmed I. Hasaballah
Keyword(s):  
Antioxidant Activity ◽  
Anticancer Activity ◽  
Cell Lines ◽  
Crude Extract ◽  
Chitosan Nanoparticles ◽  
Radical Scavenging ◽  
Nano Particles ◽  
Liver Carcinoma ◽  
Crude Extracts ◽  
Hct 116

The antioxidant effects besides anticancer activities of Musca domestica, Lucilia sericata and Chrysomya albiceps maggots extracts against human liver carcinoma (HepG-2) and human colon carcinoma (HCT-116) were investigated. Two kinds of extracts, crude and chitosan nanoparticles (CNPs) were prepared. The antioxidant activity of different tested extracts was performed by DPPH radical scavenging method, the results obtained revealed that, the highest levels of DPPH scavenging activity were exhibited by the crude extracts of tested maggots with preference to C. albiceps extract, which exhibited a much more potent activity followed by L. sericata and M. domestica in crude and CNPs extracts. Crude extracts have lower anticancer activity than the CNPs extracts; however, the lowest percentage of cell viability (6.7±0.7%) was recorded by L. sericata crude extract against HCT-116, followed by C. albiceps crude extract (7.57±1.25%) against HepG-2 at the highest used concentration 100 µg/ml. The strongest anticancer activity was observed with CNPs extracts and it was recorded at concentrations of 80, 90 and 100 µg/ml against cell lines tested. Depending on Median inhibitory concentrations (IC50) of maggots crude and CNPs extracts, the IC50 values were in the range of 37.3 to 74.3 µg/ml and the highest anticancer activity was obtained by C. albiceps CNPs extracts against cell lines tested. In conclusion, both tested extracts have optimistic antioxidant activity. CNPs extracts have great therapeutic potential due to its anticancer inducing activities.

scholarly journals Effectual Anticancer Potentiality of Loaded Bee Venom onto Fungal Chitosan Nanoparticles

2020 ◽  
Vol 2020 ◽  
pp. 1-9 ◽  
Author(s):  
Adel I. Alalawy ◽  
Haddad A. El Rabey ◽  
Fahad M. Almutairi ◽  
Ahmed A. Tayel ◽  
Mohammed A. Al-Duais ◽  
...  
Keyword(s):  
Chitosan Nanoparticles ◽  
Entrapment Efficiency ◽  
Antitumor Agent ◽  
Fungal Species ◽  
Bee Venom ◽  
Cervix Carcinoma ◽  
Fluorescent Staining ◽  
Fungal Chitosan ◽  
Dose Dependent

Chitosan and its nanoparticles (NPs) could be extracted from numerous fungal species and used as effectual carriers for bioactive compounds. The fungal chitosan (FC) was innovatively acquired from Fusarium oxysporum grown mycelia, characterized and used for NP synthesis and loading with bee venom (BV). The nano-FC (NFC) had 192.4 nm mean NP diameter, 38.22% loading capacity, and 92.42% entrapment efficiency. BV release from NFC was pH and time dependent; burst BV release was detected at the first 6 h, followed by gradual releases up to 30 h. The in vitro anticancer potentiality valuation, of NFC, BV, and NFC/BV nanoconjugates against HeLa cervix carcinoma, revealed that they all had potent dose-dependent anticancer activity; BV/NFC nanoconjugates were the most effective with IC50=200 μg/mL. The fluorescent staining of treated HeLa cells with BV/NFC nanoconjugates, with DAPI and acridine orange/propidium iodide combination, indicated the appearance of early apoptosis, secondary apoptosis, and secondary necrosis markers and their increment with exposure prolongation. The production of NFC from F. oxysporum and their loading with BV are strongly counseled for production of potent natural antitumor agent with augmented activity against cervix carcinoma.

scholarly journals Synthesis, characterization and anticancer activity of rutin loaded chitosan nanoparticles

2021 ◽  
Vol 1 (1) ◽  
pp. 24-31
Author(s):  
M.D. Imad Uddin ◽  
◽  
K. Saivani ◽  
K.V. Akhil ◽  
K. Nandini ◽  
...  
Keyword(s):  
Anticancer Activity ◽  
Chitosan Nanoparticles
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