High antibacterial activity of chitosan films with covalent organic frameworks immobilized silver nanoparticles

Abstract Silver nanoparticles (AgNPs) were synthesized using Aspergillus fumigatus (A. fumigatus) mycelia extract via the hydrothermal method. The main reducing and stabilizing groups and components of A. fumigatus extract, such as amine, hydroxyl, amid, protein, enzymes, and cell saccharide compounds, were identified by Fourier transform infrared (FT-IR). Central composition design was used to plan the experiments, and response surface methodology was applied to evaluate of the effects of independent variables, including the amount of the prepared extract (5–7 ml) and heating time (10–20 min) at 121°C and 1.5 bar), on the particle size of the synthesized AgNPs, as manifested in broad emission peak (λmax). More stable and spherical monodispersed AgNPs, with mean particle size, polydispersity index (PDI) value, and maximum ζ potential value of 23 nm, 0.270, and +35.3 mV, respectively, were obtained at the optimal synthesis conditions using 7 ml of A. fumigatus extract and heating time of 20 min. The synthesized AgNPs indicated high antibacterial activity against both Gram-positive and Gram-negative bacteria.

Download Full-text

Surface Modification of Electrospun Polyethylenimine/Polyvinyl Alcohol Nanofibers Immobilized with Silver Nanoparticles for Potential Antibacterial Applications

Current Nanoscience ◽

10.2174/1573413716999200728154652 ◽

2020 ◽

Vol 16 ◽

Author(s):

Xiangyang Shi ◽

Pengchao Liu ◽

Yunpeng Huang ◽

Xu Fang ◽

Hui Ma ◽

...

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Polyvinyl Alcohol ◽

Electrospun Nanofibers ◽

Cell Viability Assay ◽

High Antibacterial Activity ◽

Viability Assay ◽

In Vitro Antibacterial Activity ◽

Biological Protection

Objective: In order to investigate the potential biomedical applications of silver nanoparticle (Ag NP)-immobilized electrospun nanofibers with different surface functionalities. <P> Methods: silver nanoparticles were immobilized within water-stable electrospun polyethylenimine (PEI)/polyvinyl alcohol (PVA) nanofibers by an in-situ reduction method after complexing Ag+ ions with the free PEI amine groups. The obtained Ag NP-incorporated PEI/PVA nanofibers were then hydroxylated, carboxylated, and acetylated to generate different surface functionalities. Different techniques were employed to characterize the Ag NP-containing nanofibers with different surface functionalities. <P> Results: In vitro antibacterial activity tests show that Ag NP-containing nanofibrous mats have a high antibacterial activity and are capable of inhibiting the growth of both S. aureus and E. coli bacteria. Cell viability assay data show that the Ag NP-containing nanofibers are cytocompatible, and those treated by hydroxylation and acetylation display better cytocompatibility than those treated by carboxylation and the pristine non-modified fibers to promote cell adhesion and proliferation. <P> Conclusion: Therefore, the hydroxylated or acetylated Ag NP-containing PEI/PVA nanofibers have a great potential for wound dressing, biological protection and tissue engineering applications

Download Full-text

Green synthesis, size control, and antibacterial activity of silver nanoparticles on chitosan films

Research on Chemical Intermediates ◽

10.1007/s11164-019-03842-z ◽

2019 ◽

Vol 45 (9) ◽

pp. 4463-4472 ◽

Cited By ~ 4

Author(s):

Cheng-Ho Chen ◽

Yin-Chen Lin ◽

Ching-Fong Mao ◽

Wei-Tung Liao

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Green Synthesis ◽

Size Control ◽

Chitosan Films

Download Full-text

Fortunella japonica extract as a reducing agent for green synthesis of silver nanoparticles

International Journal of Engineering & Technology ◽

10.14419/ijet.v7i3.10733 ◽

2018 ◽

Vol 7 (3) ◽

pp. 1570

Author(s):

Nguyen Phung Anh ◽

Truong Thi Ai Mi ◽

Duong Huynh Thanh Linh ◽

Nguyen Thi Thuy Van ◽

Hoang Tien Cuong ◽

...

Keyword(s):

Electron Microscopy ◽

Silver Nanoparticles ◽

Antibacterial Activity ◽

Volume Ratio ◽

Diffusion Method ◽

High Antibacterial Activity ◽

Agno3 Solution ◽

Transmission Electron ◽

Vis Spectroscopy ◽

Average Size

A rapid way of synthesizing silver nanoparticles (AgNPs) by treating Ag+ ions with a green Fortunella Japonica (F.J.) extract as a combined reducing and stabilizing agent was investigated. The reaction solutions were monitored using UV-Vis spectroscopy, the size and shape of crystals were determined by scanning electron microscopy and transmission electron microscopy, the crystalline phases of AgNPs were presented by X–ray diffraction, and the relation of nanoparticles with Fortunella Japonica extract was confirmed using fourier transform infrared spectroscopy. The results indicated that no formation of AgNPs had taken place in the dark during 24 hours at room temperature and 40 oC. Meanwhile, it was found that the rate of AgNPs formation increased rapidly under the sunlight. The effects of the synthesis factors on the AgNPs formation were investigated. The suitable conditions for the synthesis of AgNPs using F.J. extract were determined as follows: F.J. extract was mixed with AgNO3 1.75 mM solution with the volume ratio of 3.5 AgNO3 solution/1.5 F.J. Extract, stirred 300 rpm for 150 minutes at 40 oC under sunlight illumination. At these conditions, AgNPs showed high crystalline structure with the average size of 15.9 nm. The antibacterial activity of silver nanoparticles was determined by agar well diffusion method against E. coli and B. subtilis bacteria. The green synthesized AgNPs performed high antibacterial activity against both bacteria.

Download Full-text

Biological Synthesis of Silver Nanoparticles by Cell-Free Extract ofSpirulina platensis

Journal of Nanotechnology ◽

10.1155/2015/132675 ◽

2015 ◽

Vol 2015 ◽

pp. 1-6 ◽

Cited By ~ 16

Author(s):

Gaurav Sharma ◽

Nakuleshwar Dut Jasuja ◽

Manoj Kumar ◽

Mohammad Irfan Ali

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Large Scale ◽

Cost Effective ◽

Biological Synthesis ◽

Cell Free Extract ◽

Tem Analysis ◽

High Antibacterial Activity ◽

Vis Spectroscopy ◽

Average Size

The present study explores biological synthesis of silver nanoparticles (AgNPs) using the cell-free extract ofSpirulina platensis. Biosynthesised AgNPs were characterised by UV-Vis spectroscopy, SEM, TEM, and FTIR analysis and finally evaluated for antibacterial activity. Extracellular synthesis using aqueous extract ofS. platensisshowed the formation of well scattered, highly stable, spherical AgNPs with an average size of 30–50 nm. The size and morphology of the nanoparticles were confirmed by SEM and TEM analysis. FTIR and UV-Vis spectra showed that biomolecules, proteins and peptides, are mainly responsible for the formation and stabilisation of AgNPs. Furthermore, the synthesised nanoparticles exhibited high antibacterial activity against pathogenic Gram-negative, that is,Escherichia coli, MTCC-9721;Proteus vulgaris, MTCC-7299;Klebsiella pneumoniae, MTCC-9751, and Gram-positive, that is,Staphylococcus aureus, MTCC-9542;S. epidermidis, MTCC-2639;Bacillus cereus, MTCC-9017, bacteria. The AgNPs had shown maximum zone of inhibition (ZOI) that is31.3±1.11inP. vulgaris. Use of such a microalgal system provides a simple, cost-effective alternative template for the biosynthesis of nanomaterials of silver in a large scale that could be of great use in biomedical applications.

Download Full-text

Investigation of Nanoparticle Metallic Core Antibacterial Activity: Gold and Silver Nanoparticles against Escherichia coli and Staphylococcus aureus

International Journal of Molecular Sciences ◽

10.3390/ijms22041905 ◽

2021 ◽

Vol 22 (4) ◽

pp. 1905

Author(s):

Jimmy Gouyau ◽

Raphaël E. Duval ◽

Ariane Boudier ◽

Emmanuel Lamouroux

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Silver Nanoparticles ◽

Antibacterial Activity ◽

Metallic Nanoparticles ◽

Catalytic Reduction ◽

Gold And Silver Nanoparticles ◽

High Antibacterial Activity ◽

Vis Spectroscopy ◽

Broth Microdilution Method

Multidrug-resistant (MDR) bacteria constitute a global health issue. Over the past ten years, interest in nanoparticles, particularly metallic ones, has grown as potential antibacterial candidates. However, as there is no consensus about the procedure to characterize the metallic nanoparticles (MNPs; i.e., metallic aggregates) and evaluate their antibacterial activity, it is impossible to conclude about their real effectiveness as a new antibacterial agent. To give part of the answer to this question, 12 nm gold and silver nanoparticles have been prepared by a chemical approach. After their characterization by transmission electronic microscopy (TEM), Dynamic Light Scattering (DLS), and UltraViolet-visible (UV-vis) spectroscopy, their surface accessibility was tested through the catalytic reduction of the 4-nitrophenol, and their stability in bacterial culture medium was studied. Finally, the antibacterial activities of 12 nm gold and silver nanoparticles facing Staphylococcus aureus and Escherichia coli have been evaluated using the broth microdilution method. The results show that gold nanoparticles have a weak antibacterial activity (i.e., slight inhibition of bacterial growth) against the two bacteria tested. In contrast, silver nanoparticles have no activity on S. aureus but demonstrate a high antibacterial activity against Escherichia coli, with a minimum inhibitory concentration of 128 µmol/L. This high antibacterial activity is also maintained against two MDR-E. coli strains.

Download Full-text

Silver Nanoparticles Stabilized Using Chitosan Films: Preparation, Properties and Antibacterial Activity

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2015.11697 ◽

2015 ◽

Vol 15 (12) ◽

pp. 10120-10126 ◽

Cited By ~ 11

Author(s):

K. Kolarova ◽

V. Vosmanska ◽

S. Rimpelova ◽

P. Ulbrich ◽

V. Svorcik

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Chitosan Films

Download Full-text

Nitroxide-coated silver nanoparticles: synthesis, surface physicochemistry and antibacterial activity

RSC Advances ◽

10.1039/c5ra09366j ◽

2015 ◽

Vol 5 (72) ◽

pp. 58403-58415 ◽

Cited By ~ 36

Author(s):

Magdalena Gozdziewska ◽

Grzegorz Cichowicz ◽

Katarzyna Markowska ◽

Katarzyna Zawada ◽

Elżbieta Megiel

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Bacterial Membrane ◽

High Antibacterial Activity ◽

Nanoparticles Synthesis

We report the synthesis of well-defined silver nanoparticles with high antibacterial activity. Nitroxides on their surface may be oxidized under the influence of ROS to oxoammonium ions which are capable of interacting with bacterial membrane.

Download Full-text

Silver-Polystyrene (Ag/PS) Nanocomposites Doped with Polyvinyl Alcohol (PVA)—Fabrication and Bactericidal Activity

Nanomaterials ◽

10.3390/nano10112245 ◽

2020 ◽

Vol 10 (11) ◽

pp. 2245

Author(s):

Anna Krzywicka ◽

Elżbieta Megiel

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Polyvinyl Alcohol ◽

Polymer Matrix ◽

Bactericidal Activity ◽

Shake Flask ◽

Silver Content ◽

Silver Ions ◽

Water Migration ◽

High Antibacterial Activity

In the present work, we report the studies on perfectly homogeneous nanocomposites composed of polystyrene-grafted silver nanoparticles (Ag@PS) as a bioactive fulfilment and a mixture of polystyrene (PS) and polyvinyl alcohol (PVA) as a matrix. The procedure developed by our group of the nanocomposites’ preparation consists of three steps: synthesis of narrow-dispersive AgNPs (5.96 ± 1.02 nm); grafting of narrowly dispersed polystyrene onto the surface of AgNPs; thermoforming with a mixture of PS/PVA. Kirby-Bauer (K-B) and Dynamic Shake Flask (DSF) assays revealed high antibacterial activity against a series of Gram(−) and Gram(+) bacteria strains of the fabricated nanocomposites at low silver content (0.5%). We showed that the doping of Ag/PS composites with PVA increases the antibacterial activity of composites. The hydrophilic component in the nanocomposites enables easier water migration inside the polymer matrix, which makes releasing silver nanoparticles and silver ions to the environment facile.

Download Full-text

Facile Synthesis of Silver Nanoparticles with High Antibacterial Activity

Materials ◽

10.3390/ma11122498 ◽

2018 ◽

Vol 11 (12) ◽

pp. 2498 ◽

Cited By ~ 3

Author(s):

Anni Feng ◽

Jiankang Cao ◽

Junying Wei ◽

Feng Chang ◽

Yang Yang ◽

...

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Antibacterial Activities ◽

Molar Ratio ◽

Facile Synthesis ◽

Gram Negative ◽

Morphology Transition ◽

Gram Positive Bacteria ◽

High Antibacterial Activity ◽

Reverse Microemulsion Method

We report on a reverse microemulsion method for the synthesis of silver nanocrystals and examine their antibacterial activities. As the molar ratio of water to sodium bis(2-ethylhexyl) sulfosuccinate (AOT) increases to 25, a morphology transition from a sphere-like nanocrystal to a wire-like one was observed. For both the gram-negative and gram-positive bacteria, the wire-like silver nanocrystal showed higher antibacterial activities. We conclude that the morphology of silver nanocrystals dominates their antibacterial activity.

Download Full-text