Development of a RAPD-PCR method for identification of Bacillus species isolated from Cheonggukjang

2009 ◽  
Vol 129 (3) ◽  
pp. 282-287 ◽  
Author(s):  
Gun-Hee Kwon ◽  
Hwang-A Lee ◽  
Jae-Young Park ◽  
Jong Sang Kim ◽  
Jinkyu Lim ◽  
...  
Keyword(s):  
Bacillus Species ◽  
Rapd Pcr ◽  
Pcr Method

WEEDS IN POTATO CULTURE AND THEIR OUTCOME IN SPREADING OF Alternaria spp.

2018 ◽  
Vol 17 (6) ◽  
pp. 159-166
Author(s):  
Halina Kurzawińska ◽  
Stanisław Mazur ◽  
Małgorzata Nadziakiewicz ◽  
Jacek Nawrocki
Keyword(s):  
Chenopodium Album ◽  
Potato Cultivar ◽  
Cirsium Arvense ◽  
Potato Plants ◽  
Potato Culture ◽  
Rapd Pcr ◽  
Microscopic Features ◽  
Weed Infestation ◽  
Pcr Method ◽  
Potato Crops

The aim of this study was to determine whether the weeds accompanying potato crops can be a source of Alternaria spp. causing Alternaria leaf blight and to determine the genetic similarities of Alternaria alternata isolates infecting selected weeds: Chenopodium album, Cirsium arvense and tested potato cultivar. Three-year field experiment was conducted on the potato cultivar ‘Vineta N’. The isolates were classified into different species on the basis of macro- and microscopic features. In each year of the study, A. alternata dominated among the isolated fungi colonizing the leaves of potato plants and the selected weeds. The genetic similarities of A. alternata isolates was determined by the RAPD-PCR method. Tested genetic forms of A. alternata were closely related; only small differences in the pattern of the separated amplification products was evidenced. The dominance of A. alternata on the weeds accompanying potato crops suggests that if weed infestation is extensive, the pathogen is very likely to spread and its population to increase.

RAPD-PCR method for detecting DNA polymorphism in Boophilus microplus (Acari: Ixodidae)

1996 ◽  
Vol 1 (0) ◽  
pp. 11 ◽  
Author(s):  
MINGYANG LAN ◽  
WEIHUA SHENG ◽  
CAOQIUN HUANG ◽  
XIANDI GU ◽  
YING JIANG ◽  
...  
Keyword(s):  
Dna Polymorphism ◽  
Boophilus Microplus ◽  
Rapd Pcr ◽  
Pcr Method

scholarly journals Characterization of the recent clinical isolates of Indian Kala-azar patients by RAPD-PCR method

2011 ◽  
Vol 35 (2) ◽  
pp. 116-122 ◽  
Author(s):  
Supriya Khanra ◽  
Subir K. Bandopadhyay ◽  
Priyanka Chakraborty ◽  
Sanchita Datta ◽  
Dinesh Mondal ◽  
...  
Keyword(s):  
Clinical Isolates ◽  
Kala Azar ◽  
Rapd Pcr ◽  
Pcr Method

scholarly journals Specialisation of Rhynchosporium secalis (Oud.) J.J. Davis infecting barley and rye

2010 ◽  
Vol 42 (No. 3) ◽  
pp. 85-93 ◽  
Author(s):  
L. Lebedeva ◽  
L. Tvarůžek
Keyword(s):  
Hordeum Vulgare ◽  
Secale Cereale ◽  
Dna Markers ◽  
Barley Variety ◽  
Effect Of Temperature ◽  
Rhynchosporium Secalis ◽  
Media Effect ◽  
Nutrient Media ◽  
Rapd Pcr ◽  
Pcr Method

Fifty-five isolates of <i>Rhynchosporium secalis</i> from <i>Hordeum vulgare</i> and 34 isolates from Secale cereale were compared for growth on different nutrient media, effect of temperature on growth and morphology of colonies. The pathogenicity of the isolates was assessed on 10 rye varieties, 10 triticale varieties and the susceptible barley variety Gambrinus. The triticale varieties differed in the number of rye chromosomes in the genome. Isozymes of <i>R. secalis</i> isolated from infected leaves of barley and rye were compared. The RAPD-PCR method was used for comparison of isolates on DNA-markers. The analysis indicated two specialised forms of the fungus; each of them able to develop only on its original host.

scholarly journals Characterization of Klebsiella pneumoniae Strains Isolated from Urinary Tract Infection: Detection of ESBL Characteristics, Antibiotic Susceptibility and RAPD Genotyping

2013 ◽  
Vol 62 (4) ◽  
pp. 401-409 ◽  
Author(s):  
MUSTAFA ONUR ALADAG ◽  
AHMET UYSAL ◽  
NIYAZI DUNDAR ◽  
YUSUF DURAK ◽  
ERDOGAN GUNES
Keyword(s):  
Urinary Tract ◽  
Klebsiella Pneumoniae ◽  
Antibiotic Susceptibility ◽  
Test Method ◽  
Penicillin G ◽  
Diffusion Method ◽  
Esbl Production ◽  
Rapd Pcr ◽  
Pcr Method ◽  
Tract Infections

In this study, a hundred Klebsiella pneumoniae strains isolated from urinary tract infections were evaluated in terms of genotyping, susceptibility to certain antibiotics and detection of extended spectrum of beta lactamase (ESBL) production. The random amplified polymorphic DNA (RAPD-PCR) method was used to identify the genetic differentiation of K. pneumoniae isolates. A total of 26 different DNA bands ranging between 334 bp and 28033 bp were detected among the strains. It was found that 100 K. pneumoniae strains revealed 11 different RAPD profiles. Antibiotic susceptibility tests were conducted using a disc diffusion method against 16 antibiotics. Fifty-five different resistance profiles were determined among the strains. ESBL-productions of the strains were determined by the double disc synergy test (DDST) and ESBL E-test methods. ESBL production rates among the strains were found to be 55% by E-test method and 45% by DDST method. While ESBL-producing K. pneumoniae strains showed the greatest resistance to penicillin G (100%), followed by piperacillin (92.7%) and erythromycin (85.4%),the resistance rates of non ESBLproducing strains to those antibiotics were determined as 97.8%, 88.8% and 88.8%, respectively. Both groups of strains showed the highest sensitivity to meropenem. Based on the results obtained from the study, it was concluded that the detection of ESBL-producing strains by the E-test method was more sensitive than by the DDST method. Phenotypic and genotypic identification methods should be used together to detect ESBL presence. The RAPD-PCR method alone will not be adequate in the genotyping of the strains and alternative DNA-based methods should be used.

scholarly journals Genetic Relationship of Four Strains of Guppy (Poeciliareticulata Peters, 1859) Using RAPD-PCR Method

2021 ◽  
pp. 15-24
Author(s):  
Ine Triana Nuradha ◽  
Ayi Yustiati ◽  
Asep Agus Handaka ◽  
Ibnu Bangkit
Keyword(s):  
Experimental Design ◽  
Genetic Relationship ◽  
Polymorphic Band ◽  
Qualitative And Quantitative ◽  
Rapd Pcr ◽  
Fish Samples ◽  
Pcr Method ◽  
Polymorphic Bands ◽  
Relationship Of ◽  
Guppy Fish

This study aims to determine the genetic relationship between four strains of guppy, albino full platinum (AFP), albino german yellow (AGY), top sword (TS) and guppy yellow cobra (GYC) using the RAPD-PCR method. This study used explorative method without experimental design and analyzed by descriptive qualitative and quantitative. The obtained genetic relationship data could be used as data reference for hybridization between strains of guppy fish that have been researched. The research was conducted in October 2020-April 2021. The three fish samples (AFP, TS and GYC) obtained from fish breeder in Cilengkrang-Bandung and AGY sample obtained from fish breeder in Tanggerang-Banten. Based on the results of amplification using OPA-03 primer (AGTCAGCCAC), four strains of guppy fish showed 30 DNA bands that included polymorphic and monomorphic bands. The AFP strains had 19 monomorphic bands, AGY had 21 DNA bands (20 monomorphic bands and one polymorphic bands), TS had 19 DNA bands (17 monomorphic bands and two polymorphic bands) and GYC had 15 DNA bands (14 monomorphic bands and one polymorphic band). Phylogenetic tree analyzed by NTSys program. It is shown between AFP and AGY strains had 95% relationship index, then between TS and GYC strains had 82% relationship index and between AFP-AGY and TS-GYC had 50% relationship index.

scholarly journals Genomic Polymorphism of Trichophyton Rubrum Isolated from Keratinized Clinical Sources using the RAPD-PCR Method

2021 ◽  
Vol 29 (1) ◽  
pp. 39-49
Author(s):  
kumarss amini ◽  
shima chehreii ◽  
parisa malekabadi ◽  
◽  
◽  
...  
Keyword(s):  
Trichophyton Rubrum ◽  
Genomic Polymorphism ◽  
Rapd Pcr ◽  
Pcr Method
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