Gene expression profiles in response to Fas stimulation in Trypanosoma cruzi-infected host cells

Abstract Background: Wolbachia is a type of intracellular symbiotic bacteria widely distributed in arthropods including most insects and nematodes. These maternally inherited bacteria can regulate the host's reproductive system in various ways for their own vertical transmission. Since the identification of Wolbachia in many insects, the relationship between Wolbachia and host has attracted great interest. Wolbachia must rely on the host cells to survive, and they can also improve the fitness of the host through a variety of ways. However, the molecular basis of interaction between Wolbachia and their host has not been well resolved so far. Results: We performed transcriptome sequencing on testis tissues of adults of Wolbachia-infected and Wolbachia-free Drosophila melanogaster. Comparison of gene expression profiles revealed 471 significantly differentially expressed genes that involved in cell metabolism, cell membrane component correlation and hydrolysis process.Conclusions: Our results show that lipid and carbohydrate metabolism are more active in Wolbachia-infected testis than in Wolbachia free testis. This work strengthens our general understanding of the Wolbachia-host intracellular relationship and may provide a new perspective for Wolbachia-mediated virus-blocking.

Download Full-text

Comparative Transcriptome Analysis of Listeria monocytogenes Strains of the Two Major Lineages Reveals Differences in Virulence, Cell Wall, and Stress Response

Applied and Environmental Microbiology ◽

10.1128/aem.02730-06 ◽

2007 ◽

Vol 73 (19) ◽

pp. 6078-6088 ◽

Cited By ~ 37

Author(s):

Patricia Severino ◽

Olivier Dussurget ◽

Ricardo Z. N. Vêncio ◽

Emilie Dumas ◽

Patricia Garrido ◽

...

Keyword(s):

Gene Expression ◽

Cell Wall ◽

Listeria Monocytogenes ◽

Wide Spectrum ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Enrichment Analysis ◽

Host Cells ◽

Ontology Term ◽

Phylogenetic Lineage

ABSTRACT Listeria monocytogenes is a food-borne, opportunistic, bacterial pathogen causing a wide spectrum of diseases, including meningitis, septicemia, abortion, and gastroenteritis, in humans and animals. Among the 13 L. monocytogenes serovars described, human listeriosis is mostly associated with strains of serovars 4b, 1/2b, and 1/2a. Within the species L. monocytogenes, three phylogenetic lineages are described. Serovar 1/2a belongs to phylogenetic lineage I, while serovars 4b and 1/2b group in phylogenetic lineage II. To explore the role of gene expression in the adaptation of L. monocytogenes strains of these two major lineages to different environments, as well as in virulence, we performed whole-genome expression profiling of six L. monocytogenes isolates of serovars 4b, 1/2b, and 1/2a of distinct origins, using a newly constructed Listeria multigenome DNA array. Comparison of the global gene expression profiles revealed differences among strains. The expression profiles of two strains having distinct 50% lethal doses, as assessed in the mouse model, were further analyzed. Gene ontology term enrichment analysis of the differentially expressed genes identified differences in protein-, nucleic acid-, carbon metabolism-, and virulence-related gene expression. Comparison of the expression profiles of the core genomes of all strains revealed differences between the two lineages with respect to cell wall synthesis, the stress-related sigma B regulon and virulence-related genes. These findings suggest different patterns of interaction with host cells and the environment, key factors for host colonization and survival in the environment.

Download Full-text

Integration of miRNA and gene expression profiles suggest a role for miRNAs in the pathobiological processes of acute Trypanosoma cruzi infection

Scientific Reports ◽

10.1038/s41598-017-18080-9 ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 8

Author(s):

Ludmila Rodrigues Pinto Ferreira ◽

Frederico Moraes Ferreira ◽

Laurie Laugier ◽

Sandrine Cabantous ◽

Isabela Cunha Navarro ◽

...

Keyword(s):

Gene Expression ◽

Trypanosoma Cruzi ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Cruzi Infection

Download Full-text

Insights into early stages of the establishment of host:algal endosymbioses: Genetic responses to live versus heat-killed algae and bacterial prey in a sponge host

10.1101/2021.06.16.448416 ◽

2021 ◽

Author(s):

Sara Camilli ◽

Vasiliki Koutsouveli ◽

Chelsea Hall ◽

Lillian Chang ◽

Oriol Sacristan-Soriano ◽

...

Keyword(s):

Gene Expression ◽

Expression Profiles ◽

Expression Patterns ◽

Gene Expression Profiles ◽

Host Cells ◽

Differentially Expressed ◽

Cellular Mechanisms ◽

Algal Symbionts ◽

Green Algal ◽

Genetic Responses

The freshwater sponge Ephydatia muelleri and its Chlorella-like green algal partner is an emerging model for studying animal:algal endosymbiosis. The sponge host is a highly tractable laboratory organism, and the symbiotic algae are easily cultured. We took advantage of these traits to experimentally interrogate fundamental questions about cellular mechanisms that govern the establishment of durable intracellular partnerships between hosts and symbionts in facultative symbioses. We modified a classical experimental approach to discern the phagocytotic mechanisms that might be co-opted to permit persistent infections, and identified genes differentially expressed in E. muelleri sponges early in the establishment of endosymbiosis. We exposed algal-free E. muelleri to live native algal symbionts, native heat-killed algae, and bacteria, and then performed RNASeq so we could compare patterns of gene expression in each treatment. We contrasted differential gene expression patterns between potential food items (bacteria and heat-killed algae) and the live native Chlorella-like symbiont. We found a relatively small but interesting suite of genes that are uniquely and differentially expressed in the host exposed to live algal symbionts, and a larger number of genes uniquely triggered by host exposure to heat-killed algae. One of the host genes, an ABC transporter that is downregulated in response to live algal symbionts, was further evaluated for its possible role in establishment of the algal symbiosis. We discuss the gene expression profiles associated with host responses to living algal cells in the context of conditions necessary for long-term residency within host cells by phototrophic symbionts as well as the genetic responses to sponge phagocytosis and immune driven pathways.

Download Full-text