Measuring the concentration of protein nanoparticles synthesized by desolvation method: Comparison of Bradford assay, BCA assay, hydrolysis/UV spectroscopy and gravimetric analysis

2021 ◽  
Vol 599 ◽  
pp. 120422
Author(s):  
Pavel Khramtsov ◽  
Tatyana Kalashnikova ◽  
Maria Bochkova ◽  
Maria Kropaneva ◽  
Valeria Timganova ◽  
...  
Keyword(s):  
Uv Spectroscopy ◽  
Method Comparison ◽  
Gravimetric Analysis ◽  
Protein Nanoparticles ◽  
Bradford Assay ◽  
Bca Assay

scholarly journals Measuring the Concentration of Protein Nanoparticles Synthesized by Desolvation Method: Comparison of Bradford Assay, BCA Assay, hydrolysis/UV Spectroscopy and Gravimetric Analysis

2020 ◽  
Author(s):  
Pavel Khramtsov ◽  
Tatyana Kalashnikova ◽  
Maria Bochkova ◽  
Maria Kropaneva ◽  
Valeria Timganova ◽  
...  
Keyword(s):  
Uv Spectroscopy ◽  
Method Comparison ◽  
Cross Linking ◽  
Gravimetric Analysis ◽  
Nanoparticle Concentration ◽  
Gelatin Nanoparticles ◽  
Protein Nanoparticles ◽  
Bradford Assay ◽  
Analytical Approaches ◽  
Bca Assay

Research paper on sunthesis of protein nanoparticles<div><br><div><b>Abstract</b></div><div>The desolvation technique is one of the most popular methods for preparing protein nanoparticles for medicine, biotechnology, and food applications. We fabricated 11 batches of BSA nanoparticles and 2 batches of gelatin nanoparticles by desolvation method. BSA nanoparticles from 2 batches were cross-linked by heating at +70 °C for 2 h; other nanoparticles were stabilized by glutaraldehyde. We compared several analytical approaches to measuring their concentration: gravimetric analysis, bicinchoninic acid assay, Bradford assay, and alkaline hydrolysis combined with UV spectroscopy. We revealed that the cross-linking degree and method of cross-linking affect both Bradford and BCA assay. Direct measurement of protein concentration in the suspension of purified nanoparticles by dye-binding assays can lead to significant (up to 50-60%) underestimation of nanoparticle concentration. Quantification of non-desolvated protein (indirect method) is affected by the presence of small nanoparticles in supernatants and can be inaccurate when the yield of desolvation is low. The reaction of cross-linker with protein changes UV absorbance of the latter. Therefore pure protein solution is an inappropriate calibrator when applying UV spectroscopy for the determination of nanoparticle concentration. Our recommendation is to determine the concentration of protein nanoparticles by at least two different methods, including gravimetric analysis.<div><br></div></div></div>

scholarly journals Measuring the Concentration of Protein Nanoparticles Synthesized by Desolvation Method: Comparison of Bradford Assay, BCA Assay, hydrolysis/UV Spectroscopy and Gravimetric Analysis

2020 ◽  
Author(s):  
Pavel Khramtsov ◽  
Tatyana Kalashnikova ◽  
Maria Bochkova ◽  
Maria Kropaneva ◽  
Valeria Timganova ◽  
...  
Keyword(s):  
Uv Spectroscopy ◽  
Method Comparison ◽  
Cross Linking ◽  
Gravimetric Analysis ◽  
Nanoparticle Concentration ◽  
Gelatin Nanoparticles ◽  
Protein Nanoparticles ◽  
Bradford Assay ◽  
Analytical Approaches ◽  
Bca Assay

Research paper on sunthesis of protein nanoparticles<div><br><div><b>Abstract</b></div><div>The desolvation technique is one of the most popular methods for preparing protein nanoparticles for medicine, biotechnology, and food applications. We fabricated 11 batches of BSA nanoparticles and 2 batches of gelatin nanoparticles by desolvation method. BSA nanoparticles from 2 batches were cross-linked by heating at +70 °C for 2 h; other nanoparticles were stabilized by glutaraldehyde. We compared several analytical approaches to measuring their concentration: gravimetric analysis, bicinchoninic acid assay, Bradford assay, and alkaline hydrolysis combined with UV spectroscopy. We revealed that the cross-linking degree and method of cross-linking affect both Bradford and BCA assay. Direct measurement of protein concentration in the suspension of purified nanoparticles by dye-binding assays can lead to significant (up to 50-60%) underestimation of nanoparticle concentration. Quantification of non-desolvated protein (indirect method) is affected by the presence of small nanoparticles in supernatants and can be inaccurate when the yield of desolvation is low. The reaction of cross-linker with protein changes UV absorbance of the latter. Therefore pure protein solution is an inappropriate calibrator when applying UV spectroscopy for the determination of nanoparticle concentration. Our recommendation is to determine the concentration of protein nanoparticles by at least two different methods, including gravimetric analysis.<div><br></div></div></div>

scholarly journals Synthesis of a Carrageenan–Iron Complex and Its Effect on Flame Retardancy and Smoke Suppression for Waterborne Epoxy

Polymers ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 1677 ◽  
Author(s):  
Na Wang ◽  
Haiwei Teng ◽  
Xinyu Zhang ◽  
Jing Zhang ◽  
Long Li ◽  
...  
Keyword(s):  
Flame Retardancy ◽  
Uv Spectroscopy ◽  
Thermo Gravimetric Analysis ◽  
Iron Complex ◽  
Smoke Suppression ◽  
Gravimetric Analysis ◽  
Cone Calorimetry ◽  
Thermo Gravimetric ◽  
Limiting Oxygen Index ◽  
Waterborne Epoxy

A k-carrageenan–iron complex (KC–Fe) was synthesized by complexation between degraded KC and FeCl3. Furthermore, KC–Fe and ammonium polyphosphate (APP) were simultaneously added into waterborne epoxy (EP) to improve its flame retardancy and smoke suppression performance. The structure and properties of KC–Fe were assessed using Fourier transform infrared spectroscopy (FTIR), ultraviolet (UV) spectroscopy, thermo gravimetric analysis (TGA), and X-ray powder diffraction analysis (XRD). The analysis showed that KC–Fe was successfully synthesized and exhibited good thermal properties with a 49% char residue at 800 °C. The enhanced flame retardancy and smoke suppression performance of waterborne epoxy were evaluated using a limiting oxygen index (LOI) and UL-94. Moreover, the flame retardancy of waterborne epoxy coated on a steel plate was also investigated using cone calorimetry. The results showed that the flame-retardant waterborne epoxy blend exhibited the best flame retardancy when the mass ratio of APP and KC–Fe was 2:1. The total heat release (THR) and total smoke production (TSP) was decreased by 44% and 45%, respectively, which indicated good fire safety performance and smoke suppression properties. Analysis of the residual char using FTIR, SEM, and elemental analysis (EDS) indicated that the action of KC–Fe was promoted by the presence of APP. The formation of a dense thermal stable char layer from an intumescent coating was essential to protect the underlying materials.

scholarly journals Preparation, Characterization and Antibacterial Activity of Pomegranate Peel Extract and Chitosan-Silver Nanoparticles Using Electrochemical Method.

2020 ◽  
Vol 17 ◽  
pp. 222-244
Author(s):  
Afaf Sarhan ◽  
M. I. Abdel Hamid ◽  
Sabrien A. Omar ◽  
R. Hanie
Keyword(s):  
Silver Nanoparticles ◽  
Uv Spectroscopy ◽  
Electrochemical Process ◽  
Gravimetric Analysis ◽  
X Ray Diffraction ◽  
Pomegranate Peel ◽  
Vis Spectroscopy ◽  
Pomegranate Peel Extract ◽  
Average Size ◽  
Peel Extract

The present work involves the development of chitosan-pomegranate peel extract and silver nanoparticles (Cs-PPE-AgNPs) using electrochemical process followed by UV irradiation reduction. Formation of silver nanoparticles characterize by UV–vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), differential thermal analysis (DTA), SEM, Swelling and thermal gravimetric analysis (TGA). The obtain nanoparticles average size was 34.6 nm. UV Spectroscopy show new peak at 405nm indicate the formation of AgNPs particles. Cs-PPE and Cs-PPE-AgNPs also demon started a relativity high antibacterial against Escherichia coli and Staphylococcus aureus bacteria.

scholarly journals Preparation, Characterization and Antibacterial Efficiency of Olive Leaves Extract and Chitosan-Silver Nanoparticles using Electrochemical Method

2019 ◽  
Vol 15 ◽  
pp. 6152-6164
Author(s):  
Moustafa Tawfik Ahmed
Keyword(s):  
Silver Nanoparticles ◽  
Uv Spectroscopy ◽  
Good Alternative ◽  
Gravimetric Analysis ◽  
X Ray Diffraction ◽  
Olive Leaves ◽  
Scanning Calorimetry ◽  
Electrochemical Approach ◽  
Vis Spectroscopy ◽  
Average Size

The present work involves the improvement of chitosan-olive leaves extract and silver nanoparticles (Cs-OLE-AgNPs) using electrochemical approach followed by UV-irradiation reduction. The formation of silver nanoparticles (AgNPs) was characterized via UV–vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), differential scanning calorimetry (DSC), EDX, thermal gravimetric analysis (TGA) and Swelling. The obtained nanoparticles average size was 36.19 nm. UV Spectroscopy show increase of peaks at 320 and 345 nm indicate the formation of a large cluster of AgNPs. Cs-OLE and Cs-OLE-AgNPs also demon started a relativity high antibacterial against both Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) bacteria. AgNPs show broadspectrum antibacterial activity at lower concentration (0.56%) and may be regarded as a good alternative therapeutic approach in the future.

Size distribution as a measure of dispersant performanceA paper submitted to the Journal of Environmental and Engineering Science.

2009 ◽  
Vol 36 (3) ◽  
pp. 540-549 ◽  
Author(s):  
Biplab Mukherjee ◽  
Brian A. Wrenn
Keyword(s):  
Size Distribution ◽  
Microscopic Examination ◽  
Uv Spectroscopy ◽  
Response Strategy ◽  
Distribution Data ◽  
Accurate Information ◽  
Volatile Components ◽  
Gravimetric Analysis ◽  
Dispersed Oil ◽  
Oil Spill Response

Study of the chemical dispersion of crude oil using size distribution methods can provide important information needed for oil-spill response strategy, which is lacking in the more commonly used mass–concentration methods. Therefore, identification of a reliable method or methods that can provide accurate information regarding droplet size and the mass of oil dispersed is necessary. This research compared four such methods. Gravimetric analysis of the oil mass dispersed was biased low due to loss of volatile components, whereas UV spectroscopy provided accurate results. Size–distribution data obtained using an optical particle counter (OPC) produced accurate estimates of oil mass dispersed, but microscopic examination produced inaccurate and poorly reproducible estimates. Although size–distribution metrics (e.g., estimates of the mean diameter of the size distribution) produced by the two methods were similar, microscopic examination gave unreliable estimates of the number concentration of dispersed oil droplets due to the relatively small number of observations relative to the OPC.

Synthesis and Structural Characterization of a Tetra-Cobalt(II)-substituted Sandwich-type Tungstophosphate, (1,3-H2dap)2H4[Co4(1,3-Hdap)2(α-BPW9O34)2]·3H2O

2012 ◽  
Vol 67 (5) ◽  
pp. 495-498 ◽  
Author(s):  
Zi-Liang Wang ◽  
Hui-Ping Xi
Keyword(s):  
Uv Spectroscopy ◽  
Thermo Gravimetric Analysis ◽  
Three Dimensional ◽  
Gravimetric Analysis ◽  
X Ray Diffraction ◽  
Thermo Gravimetric ◽  
Counter Ions ◽  
Sandwich Type ◽  
Ir And Uv Spectroscopy

A sandwich-type tungstophosphate, (1,3-H2dap)2H4[Co4 (1,3-Hdap)2(α-B-PW9O34)2] ·3H2O (1,3-dap=1,3-diaminopropane), was hydrothermally synthesized and structurally characterized by single-crystal X-ray diffraction, elemental and thermo-gravimetric analysis, and IR and UV spectroscopy. The compound presents a classical tetra-transition metal-substituted sandwich framework, [Co4(1,3-Hdap)2(α-B-PW9O34)2]8-, which contains two lacunary α-B-[PW9O34]9- Keggin units linked by a rhomblike Co4O14(1,3-dap)2 cluster lying across an inversion center. The Co2+ ions are coordinated by a mono-protonated dap ligand. Isolatedly protonated 1,3-dap units act as counter-ions interacting with the [PW9O34]9- polyanions via hydrogen bonds forming a three-dimensional framework.

Advantages of the KDS/BCA Assay over the Bradford Assay for Protein Quantification in White Wine and Grape Juice

2014 ◽  
Vol 66 (2) ◽  
pp. 227-233 ◽  
Author(s):  
Diana Gazzola ◽  
Simone Vincenzi ◽  
Gabriella Pasini ◽  
Giovanna Lomolino ◽  
Andrea Curioni
Keyword(s):  
Grape Juice ◽  
White Wine ◽  
Protein Quantification ◽  
Bradford Assay ◽  
Bca Assay

scholarly journals A Rare Two-dimensional Network Based on 2,2ʹ-Biimidazole Bridged Double [PW11O39Ni]5– Polyanions

2011 ◽  
Vol 66 (12) ◽  
pp. 1237-1240
Author(s):  
Lin-Heng Wei ◽  
Zi-Liang Wang ◽  
Ming-Xue Li
Keyword(s):  
Uv Spectroscopy ◽  
Thermo Gravimetric Analysis ◽  
Gravimetric Analysis ◽  
Brick Wall ◽  
Two Dimensional ◽  
Keggin Anion ◽  
Thermo Gravimetric ◽  
Dimensional Network ◽  
Ir And Uv Spectroscopy ◽  
Oxygen Atoms

A novel two-dimensional complex based on the mono-vacant Keggin anion [PW11O39]7−, [Ni(BIIM)3]3[{Ni(BIIM)2}2(PNiW11O39)2(BIIM)]・H2O (BIIM = 2,2ʹ-biimidazole), was hydrothermally synthesized and characterized by elemental and thermo-gravimetric analysis, IR and UV spectroscopy. Each [PW11O39]7− anion captures an Ni2+ ion forming a [PNiW11O39]5− polyanion, two of which are linked together by a BIIM ligand into a [(PNiW11O39)2(BIIM)]10− polyanion supporting two Ni(BIIM)22+ units through two terminal oxygen atoms and affording a complicated [{Ni(BIIM)2}2(PNiW11O39)2(BIIM)]6− heteropolyanion. Each of these units is further attached to four surrounding equivalent units by their own Ni(BIIM)22+ linkers into a two-dimensional brick wall-like network with (6,3) topology.

scholarly journals Anti-Corrosive Behaviour of Saccharum officinarum Extract in 15% HCl for Stainless Steel-410 Surface

2021 ◽  
Vol 33 (6) ◽  
pp. 1389-1395
Author(s):  
Nishant Bhardwaj ◽  
Pooja Sharma ◽  
Vineet Kumar
Keyword(s):  
Stainless Steel ◽  
Film Formation ◽  
Uv Spectroscopy ◽  
Surface Film ◽  
Protective Layer ◽  
Saccharum Officinarum ◽  
Industrial Applications ◽  
Computational Studies ◽  
Gravimetric Analysis ◽  
Morphological Studies

The objective of this study is to experimentally evaluate the inhibition properties of Saccharum officinarum extract in a 15% hydrochloric acidic media for stainless steel (SS-410). The gravimetric, UV spectroscopy, FTIR and surface morphological studies carried out using SEM, AFM and XRD revealed that the S. officinarum extract protected the SS 410 steel in 15% hydrochloric acidic medium. Gravimetric analysis revealed maximum 95.92 % inhibition efficiency at 4 g/L inhibitor concentration. The computational studies (quantum chemical calculations) were further utilized to understand the concept of chelation mechanism. The surface film formation and other 3D features of S. officinarum extract film over SS-410 surface was confirmed using AFM studies. Adsorption of S. officinarum extract over SS-410 surface followed Langmuir adsorption isotherm. It is clear from the theoretical and computational studies that the S. officinarum extract acted as mixed type inhibitor by adsorption of hybrid organic-inorganic inhibitor ingredients on SS-410. Thus, S. officinarum extract can develop an efficient protective layer on SS-410 that has various industrial applications.

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