scholarly journals Predictive Role of Integrin αvβ3 Imaging with 18 F-Alfatide Micro-PET in Lewis Lung Carcinoma Tumor-Bearing C57BL/6 Mice After Radiation Therapy

2017 ◽  
Vol 99 (2) ◽  
pp. E624-E625
Author(s):  
Y. Wei ◽  
S. Yuan ◽  
J. Yu ◽  
X. Hu ◽  
W. Zhao ◽  
...  
Keyword(s):  
Radiation Therapy ◽  
Lung Carcinoma ◽  
Lewis Lung Carcinoma ◽  
Integrin Αvβ3 ◽  
Lewis Lung ◽  
Tumor Bearing ◽  
Predictive Role ◽  
Carcinoma Tumor

Can 18F-alfatide micro-PET predict the radiotherapy response in Lewis lung carcinoma tumor-bearing C57BL/6 mice?

2017 ◽  
Vol 35 (15_suppl) ◽  
pp. e23011-e23011
Author(s):  
Yuchun Wei ◽  
Ning Liu ◽  
Yong Huang ◽  
Xudong Hu ◽  
Shuanghu Yuan
Keyword(s):  
Lung Carcinoma ◽  
Lewis Lung Carcinoma ◽  
Integrin Αvβ3 ◽  
Significant Negative Correlation ◽  
Lewis Lung ◽  
Positron Emission ◽  
Pet Scanning ◽  
Before And After ◽  
Predictive Role

e23011 Background: Integrin αvβ3 plays a major role in angiogenesis, tumor growth and progression. A new tracer, 18F-AL-NOTA-PRGD2, denoted as 18F-Alfatide, has been developed for positron emission tomography (PET) imaging of integrin αvβ3. This study aimed to explore the predictive role of integrin αvβ3 imaging with 18F-Alfatide PET in non-small cell lung cancer (NSCLC) for radiation and also to assess changes in tumor uptake during the course of radiotherapy. Methods: A total of 40 mice with lewis lung carcinoma (LLC) xenografts were divided into 4 groups (Ⅰ, Ⅱ, Ⅲ and Ⅳ) randomly. The Ⅰ underwent 18F-Alfatide Micro-PET scanning before and after radiotherapy, and so did the Ⅱ except for radiotherapy. The xenografts of Ⅲ were removed at the time before radiotherapy, while the Ⅳ’s were removed after radiotherapy. Tumor and muscle uptakes before and after radiotherapy of Ⅰ(SUVrad-1, SUVrad-2, SUVM-1, SUVM-2) and Ⅱ(SUVblank-1, SUVblank-2, SUVm-1, SUVm-2) depict 18F-Alfatide Micro-PET uptake. The expression of integrin αvβ3 in Ⅲ and Ⅳ xenografts was analyzed by western blotting. Results: The changes of ΔSUV1 ( SUVrad-2/SUVM-2―SUVrad-1/SUVM-1) was significantly lower than ΔSUV2 (SUVblank-2/ SUVm-2―SUVblank-1/ SUVm-1 ) (2.65±1.72 versus 5.16±2.50, P = 0.019. Overall survival (OS) of Ⅰ(56.70±25.34 day) is significantly longer than Ⅱ(35.00±5.27 day), P = 0.002. Significant negative correlation was found between ΔSUV and OS (R = 0.640, P = 0.002). Significant higher αvβ3 was observed in Ⅲ(0.52±0.09) than Ⅳ(0.41±0.07), P = 0.007. Conclusions: Decrease of ΔSUV and integrin αvβ3 during the course of radiotherapy may predict improved response in LLC xenografts and affirm the predictive role of 18F-Alfatide for radiation in NSCLC.

Neutralizing interleukin-6 in tumor-bearing mice does not abrogate behavioral fatigue induced by Lewis lung carcinoma

2021 ◽  
pp. 113607
Author(s):  
Kiersten Scott ◽  
Thien-Phan Trong ◽  
A Phillip West ◽  
Cullen M Taniguchi ◽  
Robert Dantzer
Keyword(s):  
Lung Carcinoma ◽  
Interleukin 6 ◽  
Lewis Lung Carcinoma ◽  
Lewis Lung ◽  
Tumor Bearing

Cell Proliferation Kinetics of the Intramuscularly Implanted Lewis Lung Carcinoma

1980 ◽  
Vol 66 (6) ◽  
pp. 669-675 ◽  
Author(s):  
Gianna Badaracco ◽  
Claudia Greco ◽  
Giuseppe Starace
Keyword(s):  
Lung Carcinoma ◽  
Lewis Lung Carcinoma ◽  
Early Stage ◽  
Cell Loss ◽  
Lewis Lung ◽  
Phase Duration ◽  
Cell Cycle Time ◽  
Tumor Implantation ◽  
Kinetics Of ◽  
Carcinoma Tumor

The growth kinetics of the Lewis lung carcinoma tumor was studied. The main proliferative parameters of an early stage of the growth (8th day after tumor implantation) were derived from the analysis of the growth curve and the fraction of labeled mitoses (FLM curve). The occurrence of proliferative changes due to the transplantation was confirmed. The main variations observed concern a shortening of the cell cycle time, a prolongation of the S phase duration and an increase in cell loss. A critical analysis of the results of this preliminary study is reported.

scholarly journals Nanoliposomal L-Asparaginase and Its Antitumor Activities in Lewis Lung Carcinoma Tumor-Induced BALB/c Mice

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Thi Thao Do ◽  
Thi Phuong Do ◽  
Thi Nga Nguyen ◽  
Thi Cuc Nguyen ◽  
Thi Thu Phuong Vu ◽  
...  
Keyword(s):  
Side Effects ◽  
Antitumor Activity ◽  
Cytotoxic Activity ◽  
Lung Carcinoma ◽  
Lewis Lung Carcinoma ◽  
Lewis Lung ◽  
Antitumor Activities ◽  
Antitumor Effects ◽  
Carcinoma Tumor

Although L-Asparaginase (L-ASP) is an effective chemotherapeutic agent, it has side effects such as fever, skin rashes, chills, anaphylaxis, and severe allergic reactions. Moreover, the short half-life of L-ASP reduces its antitumor activity. To reduce its side effects and broaden its pharmaceutical applications, L-ASP obtained from Pectobacterium carotovorum was subjected to liposomal conjugation. The enzyme was then loaded into liposomes using the hydrated thin-film method. The in vitro cytotoxic activity of liposomal L-ASP was evaluated with the MTT assay using cancerous cell lines, and its antitumor effects were examined in Lewis lung carcinoma (LLC) tumorized mice. The average size of the liposomes containing purified L-asparagine was 93.03 ± 0.49 nm. They had a zeta potential of –15.45 ± 6.72 mV, polydispersity index of 0.22 ± 0.02, and encapsulation efficiency of 53.99 ± 5.44%. The in vitro cytotoxic activity of liposomal L-ASP was less effective against LLC, MCF-7 (human breast carcinoma), HepG2 (human hepatocellular carcinoma), SK-LU-1 (human lung carcinoma), and NTERA-2 (pluripotent human embryonic carcinoma) cells than that of free L-ASP. However, the antitumor activity of liposomal L-ASP was significantly greater than that of untrapped L-ASP at the same doses (6 UI/mouse) in terms of tumor size (6309.11 ± 414.06 mm3) and life span (35.00 ± 1.12 days). This is the first time the antitumor activities of PEGylated nanoliposomal L-ASP have been assessed in LLC carcinoma tumor-induced BALB/c mice and showed significantly improved pharmacological properties compared to those of free L-ASP (P<0.05). Thus, nanoliposomal L-ASP should be considered for its widening applications against carcinoma tumors.

Experimental CO2 Laser Surgery on the Lewis Lung Carcinoma Tumor Model in C56BL/6 Mice

1992 ◽  
pp. 417-423 ◽  
Author(s):  
S. Rimbach ◽  
D. Wallwiener ◽  
D. Pollmann ◽  
U. Klinger ◽  
W. Stolz ◽  
...  
Keyword(s):  
Co2 Laser ◽  
Lung Carcinoma ◽  
Laser Surgery ◽  
Lewis Lung Carcinoma ◽  
Tumor Model ◽  
Lewis Lung ◽  
Co2 Laser Surgery ◽  
Carcinoma Tumor

Abstract C78: The mu opioid receptor regulates Lewis lung carcinoma tumor growth and metastasis

2009 ◽  
Author(s):  
Biji Mathew ◽  
Frances E. Lennon ◽  
Jessica Siegler ◽  
Lynnette Gerhold ◽  
Nurbek Mambetsariev ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Opioid Receptor ◽  
Lung Carcinoma ◽  
Lewis Lung Carcinoma ◽  
Mu Opioid Receptor ◽  
Lewis Lung ◽  
Mu Opioid ◽  
Tumor Growth And Metastasis ◽  
Carcinoma Tumor

Radiation and Endostatin Gene Therapy in a Lung Carcinoma Model: Pilot Data on Cells and Cytokines that Affect Angiogenesis and Immune Status

2006 ◽  
Vol 5 (2) ◽  
pp. 135-146 ◽  
Author(s):  
Xian Luo ◽  
James M. Slater ◽  
Daila S. Gridley
Keyword(s):  
Gene Therapy ◽  
Lung Carcinoma ◽  
Lewis Lung Carcinoma ◽  
Immune Status ◽  
Lewis Lung ◽  
Tumor Bearing ◽  
Cell Counts ◽  
Blood Cell Counts ◽  
Tnf Α ◽  
Ifn Γ

The dose of radiation that can be safely delivered to cancers residing in sensitive areas such as the lungs is limited by concern for normal tissue damage. Therapies that target tumor vasculature have potential to enhance the efficacy of radiotherapy, with minimal risk for toxicity. We constructed a unique plasmid, pXLG-mEndo, containing the mouse endostatin gene. A significantly greater anti-tumor effect was obtained against Lewis lung carcinoma (LLC) in mice when pXLG-mEndo was combined with radiation compared to radiation alone. Here we report results of cellular and cytokine assessments performed one day after treatment. These analyses were done to obtain baseline data on leukocytes that affect angiogenesis, as well as anti-tumor immunity, and to detect possible treatment-related toxicities. White blood cell counts were dramatically elevated in blood and spleens of untreated tumor-bearing mice, primarily due to granulocytosis. Overall, the effect of radiation was more evident than that of the plasmids (pXLG-mEndo and parental pWS4); radiosensitivity of specific lymphocyte subsets was variable (B > T > NK; CD8+ Tc > CD4+ Th). Tumor presence resulted in dramatically elevated interleukin-2 (IL-2) and decreased tumor necrosis factor-α (TNF-α) in supernatants of activated splenocytes, but had no significant effect on interferon-γ (IFN-γ). Administration of pXLG-mEndo, radiation, or both modified the tumor-induced aberrations in IL-2 and TNF-α; IFN-γ production was decreased by radiation. Red blood cell counts, hemoglobin, and hematocrit were low in tumor-bearing mice, but there were no treatment-related differences among groups. Platelet counts were reduced, whereas their volumes were increased in tumor-bearing mice; both parameters were only slightly affected by either pXLG-mEndo or control plasmid injection, however. The data demonstrate in the Lewis lung carcinoma model that tumor-localized endostatin gene therapy and radiation had significant effects on cells and cytokines that can influence angiogenesis, tumor growth, and immune status.

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