GC–MS analysis of bioactive components and synthesis of silver nanoparticle using Ammannia baccifera aerial extract and its larvicidal activity against malaria and filariasis vectors

Present study was an attempt to investigate the bioactive components present in the leaves of Guaiacum officinale using gas chromatography-mass spectroscopy (GC-MS) analysis and study the antiinflammatory potential of those constituents using molecular docking studies. GC-MS analysis was done by standard protocol using the equipment JEOL GC MATE II. The identification of components was based on NIST (National Institute of Standards and Technology) Version-11 library as well as comparison of their retention indices. The molecular docking studies were done using the commercial docking software MCULE, 1-click docking. GC-MS analysis of the alcoholic extract showed the presence of ten compounds at different retention times. The phytoconstituent 8,11,14-eicosatrienoic acid was present at high concentration with % peak area of 43.3 at a retention time of 19.43 min followed by 5,7-dihydroxy-8-methoxy flavone (Wogonin) at a retention time of 17.73. All 10 compounds obtained from GC-MS analysis and diclofenac were used as the ligands in this study, with cyclooxygenase-2 (COX-2), phospholipase A2 and interleukin receptor as the molecular targets. in silico Docking studies revealed that the flavanoid Wogonin is having highest binding potential indicated by least docking score of -8.2, -8 and -6.9 kcal/mol on COX-2, phospholipase A2 and interleukin receptor respectively.

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Py-GC/MS Analysis of Bioactive Components of 450°C Pyrolyzate from Ethanol Extractives of Oil-Tea Cake

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.480-481.513 ◽

2011 ◽

Vol 480-481 ◽

pp. 513-518

Author(s):

Qi Mei Liu ◽

Wan Xi Peng

Keyword(s):

Phosphonic Acid ◽

Value Added ◽

Bioactive Components ◽

Cyclobutanedicarboxylic Acid ◽

Ms Analysis ◽

Cosmetic Industry ◽

Analytical Result ◽

Main Components

450°C-based pyrolysis- GC/MS technology was used to analyze the bioactive components of ethanol extractives of oil-tea cake. The analytical result showed that 38 peaks were obtained from the 450°C-based pyrolyzate of ethanol extractives of oil-tea cake, and 37 compounds representing 97.81 % of the total areas were identified (Table 1). The analytical result showed that the main components of ethanol extractives of oil-tea cake by 450°C-based pyrolysis- GC/MS were as: Benzene, 1,2,3-Trimethoxy-5-Methyl- (21.56%), Phenol, 3,4-Dimethoxy- (16.31%), 3',5'-Dimethoxyacetophenone (14.37%), 1,2-Ethanediamine, N-Ethyl- (6.62%), Indole (5.39%), Mequinol (4.13%), Phosphonic Acid, (P-Hydroxyphenyl)- (2.97%), 1,2-Cyclobutanedicarboxylic Acid, Cis- (1.67%), Bicyclo 3.1.0 Hexan-3-One (1.38%), Benzene, 1,4-Dimethoxy-2,3,5,6- Tetramethyl- (1.35%), 2h-Azepin-2-One, Hexahydro-1-(2-Propenyl)- (1.15), etc. The results of function analyses showed that the 450°C-based pyrolyzate of ethanol extractives of oil-tea cake can be used as rare natural medicinal materials, and value-added materials of spice, food and cosmetic industry.

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PHYTOCHEMICAL SCREENING AND GC-MS ANALYSIS OF BIOACTIVE CONSTITUENTS OF THE HEXANE EXTRACT FROM ACANTHOSPERMUM HISPIDUM (whole plant)

Journal of Chemical Society of Nigeria ◽

10.46602/jcsn.v46i1.575 ◽

2021 ◽

Vol 46 (1) ◽

Author(s):

Akoh, O. U ◽

Mac-Kalunta O. M ◽

Emewusim, C. M

Keyword(s):

Hexane Extract ◽

Raw Material ◽

Phytochemical Screening ◽

Bioactive Components ◽

Bioactive Constituents ◽

Invasive Weed ◽

Ms Analysis ◽

Standard Procedures ◽

Whole Plant ◽

Entire Plant

Although Acanthospermum hispidum has long been considered as a competitive invasive weed of agricultural plantations, it has recently become a sought after raw material to manufacture syrups in the treatment of asthma, bronchitis and fever. In the present study, the bioactive components of Acanthospermum hispidium entire plant have been accessed via GC-MS analysis. Six compounds from the hexane extract of the whole plant were identified. The most prevalent are glycerine (100.00%), 1-benzoypiperidine (40.26%) and erythritol (15.79%). Phytochemical screening investigation carried out using standard procedures showed the presence of Alkaloids, Steriods, Saponnins, Tannin, Flavonoids, Phenol, Terpenoids.

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Larvicidal Efficacy and GC-MS Analysis of Hyptis suaveolens Leaf Extracts against Anopheles Species

International Journal of Biochemistry Research & Review ◽

10.9734/ijbcrr/2021/v30i130241 ◽

2021 ◽

pp. 8-19

Author(s):

Y. D. Dakum ◽

C. N. Amajoh ◽

A. Ombugadu ◽

G. Istifanus ◽

F. Agwom ◽

...

Keyword(s):

Larvicidal Activity ◽

Leaf Extract ◽

Mosquito Control ◽

Larval Mortality ◽

Public Health Importance ◽

Leaf Extracts ◽

Anopheles Species ◽

Ms Analysis ◽

Hyptis Suaveolens ◽

Crude Leaf Extract

Mosquitoes are the most important group of insects in terms of public health importance, which transmit serious human diseases. The continuous application of synthetic insecticides to control these mosquitoes causes development of resistance in vector species, and an adverse effect on environmental quality and non-target organisms including human health. Therefore, the use of active toxic agents from plant extracts as alternative mosquito control strategy cannot be over emphasized, as these are non-toxic, easily available at affordable prices, biodegradable and show target - specific activities against different species of vector mosquitoes. The aim of the present study was to evaluate the larvicidal efficacy of the aqueous and methanol leaf extracts of H. suaveolens against the fourth instars larvae of Anopheles species. Standard WHO protocols was adopted for the larvicidal bioassay. Larvicidal activity was evaluated at concentrations of 200, 400, 600, 800 and 1000 mg/L. The larval mortality was observed after 24 and 48hours bioassay. The results are mean of three replicates and the mortalities recorded were subjected to chi-square test and probit analysis to determine median lethal concentrations (LC50). TheGC-MS analysis ofthe methanolic leaf extract of the plantrevealed the presence of twelve compounds on the chromatogram. Among the major compounds areOleic Acid (33.33%), Octadecanoic acid (13.52%), 1,3-Cyclohexadiene-1-methanol,alpha.,2,6,6-(10.42%), 1,3-Cyclopentanediol, trans (9.60%), n-Hexadecanoic acid (9.01%) and 4-Hepten-3-one, 4-methyl (7.0%).The phytochemical screening of the aqueous and methanol leaf extracts of the plant revealed the presence of Tannins, Terpenoids, Flavonoids, Steroids, Carbohydrates, Cardiac glycoside and resins. The LC50 values estimated were 316.22mg/Lfor methanol extract and 323.59 mg/L for aqueous extract. The differences between the two extracts were however not significantly different at P>0.05. The findings also revealed that mortality was concentration dependent and both extracts showed promising larvicidal activity against Anopheles species larvae. Therefore, the crude leaf extract of the plant is recommended to be used as alternative to synthetic larvicides and further research is needed to test the activity of the plant on non-target organisms.

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SCREENING OF BIOACTIVE COMPONENTS BY GC-MS ANALYSIS AND THE STUDY ON IN VITRO ANTICANCER EFFICACY OF MICROPORUS AFFINIS (BLUME & T. NEES) KUNT.

International Research Journal of Pharmacy ◽

10.7897/2230-8407.1112104 ◽

2020 ◽

Vol 11 (12) ◽

pp. 31-34

Author(s):

S Aneesh ◽

J E Thoppil

Keyword(s):

Biological Sciences ◽

Bioactive Compounds ◽

Chemical Characterization ◽

Bioactive Components ◽

Anticancer Activities ◽

Anticancer Efficacy ◽

Ms Analysis ◽

Ethidium Bromide Staining ◽

Leading Compounds

Natural compounds with biological activity are normally present in plants, mushrooms and their natural sources. Applied mycology is one of the most stimulating and rapidly evolving areas of the biological sciences. Hence the present study focussed on exploring Microporus affinis (Blume & T. Nees) Kunt., the least explored and edible bracket fungus. Chemical characterization by GC-MS analysis resulted in the presence of 47 bioactive compounds. 9, 12- Octadecadienoic acid (Z,Z)- methyl ester, Ergosterol, Monolinolein, Thiacremonone, Stellasterol, n- Hexadecanoic acid, Ribitol, Maltol etc., were the leading compounds. Because of the presence of various bioactive compounds which have been already reported to possess antitumor, antioxidant and anticancer activities, M. affinis extract has been tested for in vitro anticancer efficacy on DLD1 cell lines (cultured in DMEM medium) using MTT assay. It resulted in the decrease of percentage of viability as the increase in concentration of the extract. Apoptosis was determined by using Acridine orange and Ethidium bromide staining. Thus, the taxa, M. affinis can be recommended for further anticancer assays for validation.

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GC-MS ANALYSIS PROFILE AND BIOACTIVE COMPONENTS OF FLOWERS OF Bergenia ciliata, A WEED OF ROCK CREVICES

JOURNAL OF WEED SCIENCE RESEARCH ◽

10.28941/pjwsr.v27i4.1012 ◽

2021 ◽

Vol 27 (4) ◽

pp. 527-535

Author(s):

Malik F. H. Ferdosi ◽

Iqra Haider Khan ◽

Arshad Javaid ◽

Hafiz M. Saeed ◽

Ifrayeem Butt ◽

...

Keyword(s):

Methyl Ester ◽

Octadecadienoic Acid ◽

Acid Methyl Ester ◽

Literature Survey ◽

Pentadecanoic Acid ◽

Bioactive Components ◽

Hexadecanoic Acid ◽

Acid Methyl ◽

Ms Analysis ◽

Hexanedioic Acid

Bergenia ciliata is a weed of hilly areas of Pakistan that generally grows in rock crevices. In the present study, flowers of this weeds were collected from Murree. The dried flowers were extracted in methanol and subjected to GC-MS analysis that showed 7 compounds in it. The predominant compound was hexanedioic acid, bis(2-ethylhexyl) ester (48.88%) followed by γ-sitosterol (22.56%). Moderately occurring compound was cyclohexane, 1,3,5-triphenyl- (12.87%). The remaining compounds namely n-hexadecanoic acid (4.97%), pentadecanoic acid, 14-methyl-, methyl ester (3.77%), 9,12-octadecadienoic acid, methyl ester, (Z,Z)- (2.94%) and 9,12,15-octadecatrienoic acid, methyl ester, (Z,Z,Z)- (2.92%) were categorized as less abundant compounds. Literature survey indicated that these compounds possess antimicrobial, larvicidal, anti-inflammatory, anticancer and/or antidiabetic properties.

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A Mosquito Larvicidal Diterpenoid Isolated from Podocarpus totara D. Don ex Lambert

Journal of Entomological Science ◽

10.18474/0749-8004-35.4.474 ◽

2000 ◽

Vol 35 (4) ◽

pp. 474-477 ◽

Cited By ~ 3

Author(s):

Sung-Eun Lee ◽

Eun-Kee Park ◽

Jeong-Gyu Kim

Keyword(s):

1H Nmr ◽

Larvicidal Activity ◽

13C Nmr ◽

Culex Pipiens ◽

Structural Elucidation ◽

Fourth Instar ◽

Root Bark ◽

Mosquito Larvicidal Activity ◽

Ms Analysis ◽

Podocarpus Totara

Totarol, a diterpenoid phenol isolated from Podocarpus totara (D. Don ex Lambert) root bark, was found to be active against second- and fourth-instar Culex pipiens Coquillett with the 24 h LC50 values of 0.25 and 0.37 μg/mL, respectively. The mosquito larvicidal activity against C. pipiens increased when bioassays were extended to 48 h. Structural elucidation of totarol was by means of 1H-NMR, 13C-NMR, and GC-MS analysis.

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