Preparation and antibacterial activity of Litsea cubeba essential oil/dandelion polysaccharide nanofiber

Litsea cubebaessential oil (LCEO) extracted from the fruit of theLitseatree is a broad-spectrum bacteriostatic agent that has been used to treat ailments for thousands of years in China.

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Antibacterial Activity and Interaction Effects of Litsea Cubeba Fruit Essential Oil and Chitosan Against Food-Borne Bacteria

JST: Engineering and Technology for Sustainable Development ◽

10.51316/jst.151.etsd.2021.31.3.1 ◽

2021 ◽

Vol 31 (3) ◽

pp. 1-6

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Inhibitory Concentration ◽

Synergistic Effects ◽

Inhibition Zone ◽

Food Preservation ◽

Individual Treatment ◽

Fractional Inhibitory Concentration ◽

Litsea Cubeba ◽

Food Borne

The aims of this study were to investigate the antibacterial activity and interaction effect of Litsea cubeba fruit essential oil (EO) and chitosan (CTS) against food-borne bacteria. The inhibition zone of EO, CTS and EO-CTS mixture against Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 14028, Staphylococcus aureus ATCC 25923 and Bacillus cereus ATCC 13061 ranged from 39.0 - 57.3 mm; 11.7 - 15.5 mm and 29.7 - 39.3 mm, respectively. The minimum inhibitory concentration (MIC) of EO and CTS were 5.53 mg/mL and from 0.60 - 0.80 mg/mL, respectively. The synergistic effects were found when EO and CTS were used in combination with fractional inhibitory concentration values ranging from 0.62 - 0.67. The MIC of the EO and CTS combination was decreased from 6 to 8-fold and 2-fold compared to individual treatment by EO and CTS, respectively. The obtained results demonstrated the potential application of EO-CTS mixture in food preservation.

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Antibacterial Activity and Mechanism of Litsea cubeba L. Essential Oil Against Acinetobacter baumannii

Natural Product Communications ◽

10.1177/1934578x21999146 ◽

2021 ◽

Vol 16 (3) ◽

pp. 1934578X2199914

Author(s):

Kaiyuan Hao ◽

Baichang Xu ◽

Geyin Zhang ◽

Feifei Lv ◽

Yuhan Wang ◽

...

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Cell Membrane ◽

Acinetobacter Baumannii ◽

Growth Curves ◽

Antibacterial Activities ◽

Dilution Method ◽

Obvious Effect ◽

Strong Activity ◽

Litsea Cubeba

The main objective of this study was to investigate the antibacterial activity and mechanism of Litsea cubeba essential oil (LCEO) against Acinetobacter baumannii. The antibacterial activity was examined by a serial dilution method and growth curves. The essential oil showed strong activity against A. baumannii. The mechanism of the antibacterial action was evaluated by the integrity and permeability of the membrane, scanning electron microscopy ( SEM) and SDS-PAGE. The change in permeability of the cell membrane and leakage of cell intracellular biomacromolecules verified that LCEO has an obvious effect on the cell membrane. SEM showed the damaging effect of the essential oil on cells since the morphology of the treated bacteria was significantly changed. Different expression of proteins indicated the effect of the essential oil on protein synthesis. It is suggested that LCEO with both emulsifying and antibacterial activities, has a potential to serve as an inhibitor against A. baumannii.

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INTERACTION EFFECTS OF LITSEA CUBEBA ESSENTIAL OIL AND ANTIBIOTICS ON ANTIBACTERIAL ACTIVITY AGAINST PATHOGENIC BACTERIA IN AQUACULTURE

Vietnam Journal of Science and Technology ◽

10.15625/2525-2518/55/5a/12180 ◽

2018 ◽

Vol 55 (5A) ◽

pp. 66

Author(s):

Hai Van Nguyen

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Nalidixic Acid ◽

Pathogenic Bacteria ◽

Interaction Effects ◽

Inhibitory Effect ◽

Antagonistic Effect ◽

E Coli ◽

Litsea Cubeba ◽

Reference Strains

In this study, the interaction effects of Litsea cubeba essential oil (EO) and two antibiotics (ABs) on the antibacterial activity against pathogenic bacteria in aquaculture were investigated. Two reference strains (Escherichia coli ATCC 25922 (E. coli ATCC 25922) and Vibrio parahaemolyticus ATCC 17802 (V. parahaemolyticus ATCC 17802)) and six locally isolated aquatic pathogenic strains (E. coli 9C48, E. coli 11C123, Vibrio 2S4, Vibrio 2N38, V. parahaemolyticus ND201 and V. parahaemolyticus TB81) were used. The inhibitory effects of individual antimicrobial agent (L. cubeba EO, nalidixic acid, and oxytetracycline) were tested against eight strains by using broth microdilution assay in 96-well microplates. A higher inhibitory effect of L. cubeba EO was observed against isolated aquatic pathogenic (MIC = 1.15 - 2.30 mg/mL) than that in the reference strains (MIC = 5.53 mg/mL). The combination effects of L. cubeba EO and ABs often used in the treatment of bacteria effects in aquaculture (nalidixic acid and oxytetracycline) were evaluated by the checkerboard method. Fractional Inhibitory Concentration (FIC) values were determined to characterize the interaction among combinations. Out of 16 AB-EO combinations tested, 11 of them showed a synergistic effect (FIC ≤ 0.5), 3 of them showed an additional effect (0.5 < FIC ≤ 1.0) and 2 of them showed indifferent effect (1 < FIC ≤ 4), no antagonistic effect was observed. The antimicrobial synergy of EO and AB could enhance efficacy, reduce toxicity, decrease adverse side effects, and lower the dose of ABs used in aquaculture.

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