Ultrasound-assisted extraction of five anthraquinones from Rheum palmatum water extract residues and the antimicrobial activities

Enzyme-assisted extraction (EAE) and ultrasound-assisted extraction (UAE) were popular methods used to extract bioactive compounds from citrus peels, by-products of fruit processing industry. In this study, the total phenolic content (TPC), total flavonoid content (TFC), naringin and hesperidin contents, and antioxidant and antimicrobial activities of the extracts from pomelo peels using the combined enzyme and ultrasound-assisted extraction (E-UAE) or ultrasound and enzyme-assisted extraction (U-EAE) technique were investigated and compared with those extracted using the EAE and UAE. The optimal EAE conditions were as follows: enzyme concentration of 2%, water-solid ratio of 40 ml/g, incubation temperature of 50°C, and extraction time of 60 min, whereas the optimal UAE conditions were ultrasonic energy of 40 kHz, water-solid ratio of 40 ml/g at room temperature, and extraction time of 60 min. The results indicate that the total phenolics, total flavonoids, naringin, and hesperidin contents of the extracts significantly increased in the following order of the extraction techniques: UAE < EAE < U-EAE < E-UAE (p<0.05). The combined E-UAE technique was the most effective technique for bioactive compound extraction with the highest antioxidant and antimicrobial activities. The results also indicate that chemical composition and antioxidant and antimicrobial activities of the extracts were different depending on the pomelo species.

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Composition Analysis of Essential Oil from Melaleuca bracteata Leaves Using Ultrasound-assisted Extraction and its Antioxidative and Antimicrobial Activities

BioResources ◽

10.15376/biores.13.4.8488-8504 ◽

2018 ◽

Vol 13 (4) ◽

Cited By ~ 3

Author(s):

Yongyu Li ◽

Zhengmei Ye ◽

Wenting Wang ◽

Chao Yang ◽

Jingyuan Liu ◽

...

Keyword(s):

Essential Oil ◽

Antimicrobial Activities ◽

Composition Analysis ◽

Ultrasound Assisted Extraction ◽

Assisted Extraction ◽

Ultrasound Assisted

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Antioxidant and antimicrobial activities of yarrow (Achillea biebersteinii and A. wilhelmsii ) extract obtained by ultrasound-assisted extraction method

Clinical Biochemistry ◽

10.1016/j.clinbiochem.2011.08.891 ◽

2011 ◽

Vol 44 (13) ◽

pp. S354

Author(s):

Davoud Salar Bashi ◽

Bibi Sedigheh Fazly Bazzaz ◽

Amirhossein Sahebkar ◽

Mohamad Mahdi Karimkhani ◽

Abbas Ahmadi

Keyword(s):

Extraction Method ◽

Antimicrobial Activities ◽

Ultrasound Assisted Extraction ◽

Assisted Extraction ◽

Achillea Biebersteinii ◽

Ultrasound Assisted ◽

Antioxidant And Antimicrobial Activities

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Effects of Solvent and pH on Stingless Bee Propolis in Ultrasound-Assisted Extraction

AgriEngineering ◽

10.3390/agriengineering2020020 ◽

2020 ◽

Vol 2 (2) ◽

pp. 308-316

Author(s):

Fung Chun Chong ◽

Lee Suan Chua

Keyword(s):

Mass Spectrometry ◽

Antioxidant Capacity ◽

Radical Scavenging ◽

Water Extract ◽

Ethanolic Extract ◽

Gas Chromatography Mass Spectrometry ◽

Ultrasound Assisted Extraction ◽

Ph Values ◽

Assisted Extraction ◽

Ultrasound Assisted

Ultrasound-assisted extraction was used to extract propolis from a dark and resinous substance harvested from a beehive of Heterotrigona itama, which is commonly known as stingless bees. The propolis extracts were prepared using ethanol and water at different pH values of 3, 6, and 9. The yield of the ethanolic extract was significantly higher than the water extract, but there were no significant differences at different pH values. The ethanolic extract was found to have a lower 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity than the water extract at pH values of 6 and 9. However, the acidic propolis extracts, particularly the ethanolic extract, were found to have the highest antioxidant capacity. The addition of 20% polyethylene glycol 400 in the solvent systems was unlikely to improve propolis extraction. This can be seen from the antioxidant capacity and metabolite profile of the propolis extracts. Gas chromatography–mass spectrometry (GC–MS)-based high throughput screening of the propolis extracts showed them to have small metabolites of hydrocarbons, esters, terpenes, and alkaloids, as well as high antioxidative 2,4-di-tert-butylphenol. The detection of mangostin, mangiferin, and a few flavanones in the acidic ethanolic extract by liquid chromatography tandem mass spectrometry LC–MS/MS proved its high antioxidant capacity compared to the water extract.

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Comparison of the antimicrobial activity of propolis extracts obtained by means of various extraction methods

Journal of Food Science and Technology ◽

10.1007/s13197-019-04009-9 ◽

2019 ◽

Vol 56 (12) ◽

pp. 5386-5395 ◽

Cited By ~ 8

Author(s):

Katarzyna Pobiega ◽

Karolina Kraśniewska ◽

Dorota Derewiaka ◽

Małgorzata Gniewosz

Keyword(s):

Antimicrobial Activity ◽

Extraction Methods ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Total Phenol Content ◽

Ultrasound Assisted Extraction ◽

Disk Diffusion Method ◽

Propolis Extract ◽

Assisted Extraction ◽

Ultrasound Assisted

Abstract The objective of the study was to compare the antimicrobial activities of ethanolic propolis extracts obtained using different extraction methods. Extraction of propolis was carried out using 70% ethanol, propolis to ethanol ratios of 1:10 and 1:5, extraction times of 1 or 7 days, and shaking extraction (SE), ultrasound-assisted extraction (UAE), and ultrasound-assisted shaking extraction (SUAE) methods. A total of 12 propolis extract lyophilizates were obtained. Samples were tested for extraction yield and for total phenol content by the Folin–Ciocalteau colourimetric method, and total flavonoid content using a spectrophotometric method. GLC/MS was used for the identification of chemical compounds in selected extract lyophilizates. Antimicrobial activity against selected bacterial and fungal species was assessed using the disk diffusion method. Propolis extracts obtained as the result of 1-day and 7-day shaking extraction followed by 20 min of ultrasound-assisted extraction (SUAE) had better antimicrobial properties as compared to those obtained by SE or UAE alone. SE and UAE gave lower extraction yields as well as lower phenol and flavonoid contents compared to SUAE. No differences were observed with regard to the qualitative composition of extracts obtained by any of the methods. It is best to obtain the extract using the combined method of 1-day extraction and 20-min sonication.

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The Use of Response Surface Methodology to Optimize the Ultrasound-Assisted Extraction of Five Anthraquinones from Rheum palmatum L.

Molecules ◽

10.3390/molecules16075928 ◽

2011 ◽

Vol 16 (7) ◽

pp. 5928-5937 ◽

Cited By ~ 33

Author(s):

Li-Chun Zhao ◽

Jian Liang ◽

Wei Li ◽

Kun-Mu Cheng ◽

Xianghua Xia ◽

...

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Ultrasound Assisted Extraction ◽

Rheum Palmatum ◽

Assisted Extraction ◽

Ultrasound Assisted

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Innovative Extraction Techniques for the Optimum Extraction of Phenolic Compounds from Peanut Meal and Evaluation of Their Biological Activities

Advances in Nutrition & Food Science ◽

10.33140/anfs.04.02.2 ◽

2019 ◽

Vol 4 (2) ◽

Keyword(s):

Phenolic Compounds ◽

Carcinoma Cell ◽

Biological Activities ◽

Peanut Meal ◽

Ultrasound Assisted Extraction ◽

Carcinoma Cell Lines ◽

Assisted Extraction ◽

Phenolic Extract ◽

Ultrasound Assisted ◽

Water Ratio

There is a worldwide demand for phenolic compounds (PC) because they exhibit several biological activities. This work aimed at extracting phenolic compounds from peanut meal. The methods of extraction were mainly: conventional solvent extraction (traditional methods) and ultrasound assisted extraction (recent methods) and comparing their results. Peanut meal (PM) was prepared by defatting with n-hexane, and then extracted by the two previous methods. First, the conventional solvents used were 80% methanol, ethanol, acetone, isopropanol, and distilled water. Then studied Different parameters such as meal: water ratio, also the effect of temperature and the pH on the extraction process. Second, ultrasonic assisted extractions (USAE), the parameters investigated were temperature, time and speed of sonication. Finally, all the extracts were analyzed by HPLC for their phenolic contents. Results indicated that the highest extracted PC achieved by solvents was in distilled water where 1:100, Meal: Water ratio which extracted 40 mg PC / g PM at 30& 35°C. Highest extracted PC was achieved by alkaline medium at pH 12 more than acidic and neutral medium. While (USAE) at speed 8 ultrasonication and temperature 30ᵒC, extracted 49.2mg PC /g PM. Sothe ultrasound assisted extraction exhibited great influence on the extraction of phenolic compounds from peanut meal. The ultrasonic peanut extract was examined for its antioxidant, antimicrobial and anticarcinogenic activities. The antioxidant activity of PM phenolic extract prepared by ultrasonic technique, was measured by, β-carotene, and DPPH methods, and reducing antioxidant power. Results revealed values: 84.57, 57.72 and 5960 respectively. The PM extract showed different levels of antimicrobial activity against the pathogenic bacteria used. As for the anticarcinogenic effect PM phenolic extract most effective on inhibiting colon carcinoma and lung carcinoma cell lines with IC50 = 20.7 and 20.8 µ/ml., respectively. This was followed by intestinal carcinoma and liver carcinoma cell lines with IC50= 39.6 and 40.2µ/ml.

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